ABSTRACT
5-Hydroxy-2-methoxyxanthone (1), 2-hydroxy-3-methoxyxanthone (2), trans-kielcorin (3), 4-hydroxy-3-methoxyphenyl ferulate (4) and 3beta-O-caffeoylbetulinic acid (5) were isolated from Hypericum hookerianum. Compounds 1-5 were tested against the growth of three human tumor cell lines, MCF-7, NCI-H460 and SF-268. Compounds 4 and 5 exhibited significant inhibitory activity effects against all three; GI50 values for 4 were 15.1 +/- 1.6, 18.7 +/- 2.3 and 15.9 +/- 2.7 and for 5 12.2 +/- 2.4, 19.6 +/- 2.3 and 24.3 +/- 2.5. Compound 3 was less active with GI50 values of 55.1 +/- 2.3, 49.7 +/- 3.0 and 40.5 +/- 1.5, while 1 and 2 exhibited only weak effects. Compounds 4 and 5 were moderately effective in influencing the mitogenic response to human lymphocytes to hemoagglutinin, with IC values of 26.1 +/- 3.6 and 40.8 +/- 4.9, respectively.
Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Hypericum , Phytotherapy , Plant Extracts/pharmacology , Antineoplastic Agents, Phytogenic/administration & dosage , Antineoplastic Agents, Phytogenic/therapeutic use , Cell Line, Tumor/drug effects , Cell Proliferation/drug effects , Cinnamates/administration & dosage , Cinnamates/pharmacology , Cinnamates/therapeutic use , Esters/administration & dosage , Esters/pharmacology , Esters/therapeutic use , Humans , Inhibitory Concentration 50 , Lymphocytes/drug effects , Plant Extracts/administration & dosage , Plant Extracts/therapeutic use , Xanthones/administration & dosage , Xanthones/pharmacology , Xanthones/therapeutic useABSTRACT
A new friedolanostane, 7, and three triterpenes, 8, 9a, and 10, possessing the new 11(10-->8)-abeolanostane carbon skeleton were isolated from the bark of Garcinia speciosa. Structures were elucidated by spectroscopic and spectrometric studies and the structure of 8 by X-ray crystallographic analysis, thus forcing structure revision of a triterpene from the same source previously assumed to be a friedolanostane. These and several friedo- and lanostanes earlier isolated from the same source were evaluated for cytotoxicity against three human cell lines. Most were moderately active, with three friedolanostanes effective in inducing apoptosis in the MCF-7 cell line.
