Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning.

A total of 64 piglets were used in a 35-day study to evaluate whether creep feeding piglets on the sow or super-dosing phytase to piglets post-weaning can be used as a tool to reduce stress and support adaption to weaning. Treatments consisted of creep or no creep feed being offered pre-weaning and with or without phytase supplementation at 2000 FTU/kg post-weaning. Blood samples were collected from eight piglets per treatment on days 0 (weaning), 7 and 21 post-weaning to determine plasma cortisol and myo-inositol concentrations. Four piglets per treatment (n = 16) were administered Heidelberg pH capsules 1 week prior to weaning, on the day of weaning, as well as 7 days and 21 days post-weaning, with readings monitored over a 3 h period. In the first week post-weaning, creep-fed piglets had higher daily gains (0.23 vs. 0.14 kg/d, p < 0.05) and a lower feed conversion ratio (FCR, 0.99 vs. 1.35, p < 0.01), compared to non-creep-fed pigs. At 21 days post-weaning, irrespective of creep feed, phytase supplementation reduced FCR (1.10 vs. 1.18, p = 0.05) of piglets. Average real-time stomach pH was lower in creep-fed piglets at 1 week prior to weaning (pH 3.2 vs. 4.6, p < 0.001) and on day of weaning (pH 3.1 vs. 3.7, p < 0.01). Following weaning, phytase reduced average stomach pH of piglets at days 7 (pH 2.6 vs. 3.3, p < 0.001) and 21 (pH 2.2 vs. 2.6, p < 0.01). Both cortisol and myo-inositol concentrations in plasma decreased with age; however, cortisol levels were unaffected by either treatment. Plasma myo-inositol concentrations were higher in creep-fed piglets at day of weaning (p < 0.05) and with phytase super-dosing on day 21 (p < 0.001). These findings demonstrate that both creep feeding and phytase super-dosing are useful practices to encourage better adaption to weaning and support piglet performance. This response was not related to reduced stress in piglets, as determined by cortisol levels, but instead appears to relate to improved gastric conditions for digestion, phytate degradation and myo-inositol provision in piglets.

Dietary xylanase and live yeast supplementation influence intestinal bacterial populations and growth performance of piglets fed a sorghum-based diet.

This study was to evaluate the effect of xylanase supplementation and the addition of live yeast, Saccharomyces cerevisiae, on growth performance and intestinal microbiota in piglets. One hundred and eighty commercial crossbred 23-d-old piglets (PIC 417) were sorted by initial BW and allocated to 3 treatments: control (CTR) diet, CTR diet supplemented with xylanase at 16,000 birch xylan units/kg (XYL) and XYL diet supplemented with live yeast (2 × 1010 CFU/g) at 1 kg/t (XYL + LY). Each treatment had 10 replicates, with 6 animals each. A sorghum-based diet and water were available ad libitum for 42 d of the study. Average daily gain (ADG) and average daily feed intake (ADFI) were measured from 0 to 42 d (23- to 65-d-old) and feed conversion ratio (FCR) calculated. At the end of the study, bacterial identification through 16S rRNA (V3 to V4) sequencing of the ileal and caecal digesta from one piglet per replicate was performed. No treatment effects were observed on ADFI. Pigs offered the live yeast in addition to the xylanase had increased ADG compared with those supplemented with xylanase alone (XYL + LY vs. XYL; P = 0.655). FCR was improved with XYL and XYL + LY compared with CTR (P = 0.018). Clostridiaceae counts in the ileum tended to reduce by 10% with XYL and 14% with XYL + LY compared to CTR (P = 0.07). XYL and XYL + LY increased the counts of Lactobacillaceae in the caecum compared with CTR (P < 0.0001). Dietary supplementation of live yeast combined with xylanase improved growth performance and microbial balance of piglets during the nursery phase.

Effect of live yeast supplementation to gestating sows and nursery piglets on postweaning growth performance and nutrient digestibility.

The objectives of the present study were to determine the effects of live yeast (LY) supplementation of sows during gestation and lactation and to determine the effects of supplementation of their offspring after weaning on growth performance and nutrient digestibility. A total of 40 sows were assigned to 2 dietary treatments (control vs. LY) based on parity and expected farrowing date. Birth weight, weaning weight, litter size, and mortality were measured. After weaning, 128 mixed-sex piglets (64 from each sow treatment) were selected based on their source litter and initial BW, and randomly assigned to 2 treatments (control or LY) at 4 pigs per pen (total of 32 pigs per treatment) for a 6-wk growth performance study. At the end of the growth performance trial, 2 barrows from each pen were moved to metabolism crates for total fecal collection for a digestibility trial. Addition of LY to the sow diets had no effects on birth weight, weaning weight, litter size at birth, and mortality. Piglets had greater BW on days 21 and 42 post-weaning when sows were fed diets supplemented with LY, and overall ADG was greater in piglets from sows that received LY (P < 0.05). There was no effect of sow and nursery diets on overall ADFI and G:F intake. Supplementing diets with LY during the nursery phase increased apparent total tract digestibility (ATTD) of DM, GE, and phosphorus (P) during this phase. The ATTD of GE was also greater in piglets from sows that received LY. In conclusion, LY supplementation of diets during gestation and lactation and during the nursery phase could increase ADG and ATTD of DM, GE, and P in the offspring, and this may lead to a greater lifetime growth performance in the offspring.

Effects of super-dosing phytase and inositol on growth performance and blood metabolites of weaned pigs housed under commercial conditions1.

A total of 2,156 weaned pigs (6.75 ± 0.11 kg BW) were used in a 42-d study to evaluate whether improvements in growth performance associated with super-dosing phytase can be explained by the complete dephosphorylation of phytate and liberation of inositol. Two phytase doses (0 and 2,500 FTU/kg) and 3 inositol concentrations (0%, 0.15%, and 0.30%) were combined to create 6 dietary treatments in a 2 × 3 factorial arrangement. Pigs were fed a 3-phase feeding program, with periods being 10, 10, and 22 d, respectively. Blood samples were collected on days 0, 21, and 42 from a subset of 48 pigs to analyze mineral and myo-inositol concentrations. During Phase 1, super-dosing phytase tended to improve ADG compared with pigs fed diets without phytase (P = 0.09). Increasing concentrations of inositol improved the efficiency of gain in pigs fed diets without phytase (1,022.1, 1,040.9, and 1,089.2 g/kg), but not diets with phytase (1,102.2, 1,087.2, and 1,076.2 g/kg), and this improvement was equivalent to that observed with super-dosing phytase in the absence of inositol (interaction, P = 0.015). During Phase 2, super-dosing phytase improved ADG (P = 0.001), resulting in heavier BW (P = 0.007). During Phase 3 and overall, inositol supplementation increased ADG and ADFI in a quadratic manner (P < 0.10), with the highest ADG and ADFI observed for pigs fed 0.15% of inositol. Super-dosing phytase increased serum Zn on day 21, but not on day 42 (interaction, P = 0.008), increased serum Cu (P = 0.01), but decreased serum Fe (P = 0.02). Plasma myo-inositol increased linearly from 66.9 to 97.1 and 113.2 nmol/mL with increasing inositol (P < 0.001). When plasma myo-inositol was analyzed within the subgroup of pigs fed diets without added inositol, super-dosing phytase increased plasma myo-inositol from 57.81 to 76.05 nmol/mL (0 and 2,500 FTU/kg, respectively; P = 0.05). Results demonstrate that exogenous inositol improved efficiency of gain in weaned pigs to the same level as that observed with super-dosing phytase, but this occurred only during the first 10 d of the nursery period. This suggests that the improvement in efficiency of growth when applying super-dosing phytase could be linked, in part, to complete dephosphorylation of phytate and liberation of myo-inositol, and that myo-inositol had a greater metabolic impact in piglets immediately after weaning. Consequently, myo-inositol may be a conditionally essential nutrient for young pigs during weaning stress, but further research is needed to prove this hypothesis.

The impact of epidermal growth factor supernatant on pig performance and ileal microbiota.

Weaning of pigs can lead to low-feed intake resulting in a lag in growth performance, reduced gut health, and diarrheal diseases. Epidermal growth factor (EGF), the most abundant growth factor in milk, increased weaned pig BW gain and feed efficiency in our previous work. It is believed that intestinal microbiota plays an important role in gut health and pig growth, but limited data are available on the impact of feed additives, such as EGF, on the microbial communities of the intestines. The objective of the study was to investigate if the positive influence of EGF supplementation on weight gain and gut health was related to differences in intestinal microbiota. To examine the efficacy of EGF, a 21-d animal trial was performed using 72 pigs (two equal blocks of 36 pigs with three barrows and three gilts/pen). Pigs were assigned to one of two dietary treatments at weaning (20 ± 2 d of age; n = 6 pens/treatment) balancing across treatment for litter, gender, and initial BW. Recombinant yeast supernatant containing EGF at 120 µg/kg BW/d and without EGF (control) was added to the feed for 21 d, followed by a common diet for 7 d. Pig performance was measured weekly and ileal digesta was collected at day 21 from six pigs/treatment for microbiome analysis. Pigs fed diets containing EGF fermentation supernatant had greater (P = 0.01) daily gain in week 3 and overall resulting in heavier (P = 0.029) BW at day 28, which was consistent to our previous finding. No difference in alpha-diversity (Chao1, Shanon, and Simpson indices) and beta-diversity (weighted and unweighted UniFrac distances) of ileal digesta microbiota between EGF supplemented and control pigs were observed. The relative abundances of bacterial taxa did not differ among treatment groups at the phylum level. The relative abundances of Corynebacterium (0.0 vs. 0.9%), Blautia (0.003 vs. 0.26%), and Coprococcus (0.0 vs. 0.05%) genera, and Rumminococcaceae family (0.001 vs. 0.08%) were decreased (P < 0.05) in EGF group compared to control and were negatively correlated (P < 0.05, r > 0.60) with growth performance. Pathways related to detoxification and carbohydrate metabolism were differentially represented in the luminal bacterial populations. The improved growth of pigs supplemented with EGF supernatant produced by Pichia pastoris may be related to changes in functional capacity of the gut microbial populations. However, the impact on mucosa-associated or large intestinal communities is still unknown.