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1.
Chem Mater ; 35(16): 6364-6373, 2023 Aug 22.
Article in English | MEDLINE | ID: mdl-37637013

ABSTRACT

The development of new high-performing battery materials is critical for meeting the energy storage requirements of portable electronics and electrified transportation applications. Owing to their exceptionally high rate capabilities, high volumetric capacities, and long cycle lives, Wadsley-Roth compounds are promising anode materials for fast-charging and high-power lithium-ion batteries. Here, we present a study of the Wadsley-Roth-derived NaNb13O33 phase and examine its structure and lithium insertion behavior. Structural insights from combined neutron and synchrotron diffraction as well as solid-state nuclear magnetic resonance (NMR) are presented. Solid-state NMR, in conjunction with neutron diffraction, reveals the presence of sodium ions in perovskite A-site-like block interior sites as well as square-planar block corner sites. Through combined experimental and computational studies, the high rate performance of this anode material is demonstrated and rationalized. A gravimetric capacity of 225 mA h g-1, indicating multielectron redox of Nb, is accessible at slow cycling rates. At a high rate, 100 mA h g-1 of capacity is accessible in 3 min for micrometer-scale particles. Bond-valence mapping suggests that this high-rate performance stems from fast multichannel lithium diffusion involving octahedral block interior sites. Differential capacity analysis is used to identify optimal cycling rates for long-term performance, and an 80% capacity retention is achieved over 600 cycles with 30 min charging and discharging intervals. These initial results place NaNb13O33 within the ranks of promising new high-rate lithium-ion battery anode materials that warrant further research.

3.
J Anim Ecol ; 90(3): 574-584, 2021 03.
Article in English | MEDLINE | ID: mdl-33179773

ABSTRACT

Conservation and management increasingly focus on connectivity, because connectivity driven by variation in immigration rates across landscapes is thought to be crucial for maintaining local population and metapopulation persistence. Yet, efforts to quantify the relative role of immigration on population growth across the entire range of species and over time have been lacking. We assessed whether immigration limited local and range-wide population growth of the endangered snail kite Rostrhamus sociabilis in Florida, USA, over 18 years using multi-state, reverse-time modelling that accounts for imperfect detection of individuals and unobservable states. Demographic contributions of immigration varied depending on the dynamics and geographic position of the local populations, were scale-dependent and changed over time. By comparing the relative contributions of immigration versus local demography for periods of significant change in local abundance, we found empirical evidence for a disproportionately large role of immigration in facilitating population growth of a centrally located population-a connectivity 'hub'. The importance of connectivity changed depending of the spatial scale considered, such that immigration was a more important driver of population growth at small versus large spatial scales. Furthermore, the contribution of immigration was much greater during time periods when local population size was small, emphasizing abundance-dependent rescue effects. Our findings suggest that efforts aimed at improving local breeding habitat will likely be most effective at increasing snail kite population growth. More broadly, our results provide much needed information on the role of connectivity for population growth, suggesting that connectivity conservation may have the greatest benefits when efforts focus on centrally located habitat patches and small populations. Furthermore, our results highlight that connectivity is highly dynamic over time and that interpreting the effects of connectivity at local scales may not transfer to region-wide dynamics.


Subject(s)
Birds , Population Growth , Animals , Ecosystem , Florida , Population Density , Population Dynamics
4.
Nat Ecol Evol ; 2(1): 108-115, 2018 Jan.
Article in English | MEDLINE | ID: mdl-29180705

ABSTRACT

Invasive exotic species are spreading rapidly throughout the planet. These species can have widespread impacts on biodiversity, yet the ability for native species, particularly long-lived vertebrates, to respond rapidly to invasions remains mostly unknown. Here we provide evidence of rapid morphological change in the endangered snail kite (Rostrhamus sociabilis) across its North American range with the invasion of a novel prey, the island apple snail (Pomacea maculata), a much larger congener of the kite's native prey. In less than one decade since invasion, snail kite bill size and body mass increased substantially. Larger bills should be better suited to extracting meat from the larger snail shells, and we detected strong selection on increased size through juvenile survival. Using pedigree data, we found evidence of both genetic and environmental influences on trait expression and discovered that additive genetic variation in bill size increased with invasion. However, trends in predicted breeding values emphasize that recent morphological changes have been driven primarily by phenotypic plasticity rather than micro-evolutionary change. Our findings suggest that evolutionary change may be imminent and underscore that even long-lived vertebrates can respond quickly to invasive species. Furthermore, these results highlight that phenotypic plasticity may provide a crucial role for predators experiencing rapid environmental change.


Subject(s)
Animal Distribution , Falconiformes/anatomy & histology , Falconiformes/physiology , Introduced Species , Phenotype , Snails/physiology , Animals , Biological Evolution , Falconiformes/genetics , Female , Florida , Food Chain , Male , Predatory Behavior
5.
Genome Announc ; 5(45)2017 Nov 09.
Article in English | MEDLINE | ID: mdl-29122874

ABSTRACT

Here, we report the draft genome sequences of three laboratory variants of Bacillus anthracis Sterne and their double (Δlef Δcya) and triple (Δpag Δlef Δcya) toxin gene deletion derivatives.

7.
Gait Posture ; 54: 255-258, 2017 05.
Article in English | MEDLINE | ID: mdl-28371738

ABSTRACT

OBJECTIVES: The degree of abnormality of the gait pattern of children with bilateral spastic cerebral palsy (BSCP) can be reduced by lower limb orthopaedic surgery. However, little attention is paid to the effects of surgery on standing posture. Here, we investigated the abnormality of standing posture in young people with BSCP as well as the effects of surgery on standing posture. METHODS: We have developed an index of standing posture, the Standing Posture Score (SPS), which is similar in composition to the gait profile score (GPS). We applied SPS retrospectively to 32 typically developing children and 85 children with BSCP before and after surgery to the lower limbs aimed at improving gait. We investigated the relationship between SPS and GPS before surgery and also the relationship between changes in these variables before and after surgery. RESULTS: SPS is significantly higher in young people with BSCP. SPS reduces after surgery and this reduction is correlated with the reduction in GPS. INTERPRETATION: Successful surgery improves the alignment of the lower limbs in BSCP in standing and may have a positive impact on the activities of daily living which depend on a stable and efficient standing posture.


Subject(s)
Cerebral Palsy/physiopathology , Lower Extremity/surgery , Posture/physiology , Activities of Daily Living , Adolescent , Cerebral Palsy/complications , Cerebral Palsy/surgery , Child , Female , Gait/physiology , Gait Disorders, Neurologic/etiology , Gait Disorders, Neurologic/physiopathology , Gait Disorders, Neurologic/surgery , Humans , Male , Retrospective Studies
8.
Ecol Appl ; 26(7): 1952-1968, 2016 Oct.
Article in English | MEDLINE | ID: mdl-27755742

ABSTRACT

Identifying impacts of non-native species on native populations is central to conservation and ecology. While effects of non-native predators on native prey populations have recently received much attention, impacts of introduced prey on native predator populations are less understood. Non-native prey can influence predator behavior and demography through direct and indirect pathways, yet quantitative assessments of the relative impacts of multiple, potentially counteracting, effects on native predator population growth remain scarce. Using ≈20 years of range-wide monitoring data, we tested for effects of a recently introduced, rapidly spreading non-native prey species (Pomacea maculata) on the behavior and demography of the endangered Snail Kite (Rostrhamus sociabilis). Previous studies found that food-handling difficulties caused by the large size of P. maculata (relative to the native P. paludosa) can lead to energetic deficiencies in juvenile kites, suggesting the potential for evolutionary traps to occur. However, high densities of P. maculata populations could facilitate kites by providing supplemental food resources. Contrary to prior hypotheses, we found that juvenile apparent survival increased ≈50% in wetlands invaded by non-native snails. Breeding rates and number of young fledged/successful nests were also positively associated with non-native snail presence, suggesting direct trophic benefits to kites. We found no direct effects of the invasive snail on adult survival or daily nest survival rates. Kite movements and breeding distribution closely tracked the spread of non-native snail populations. Since 2005, kites have been heavily concentrated in northern regions where non-native snails have established. This geographic shift has had hidden costs, as use of northern regions is associated with lower adult survival. Despite negative impacts to this key vital rate, matrix population modeling indicated that the multifarious effects of the non-native snail invasion on kites culminated in increased population growth rates, likely lowering short-term extinction risks. Results suggest that considering only particular components of behavior or demography may be inadequate to infer the population-dynamic importance of non-native prey on native predators, including their role in creating potential evolutionary traps. Our findings provide information pertinent to Everglades restoration, highlighting potential management trade-offs for non-native species that may aid imperiled species recovery yet disrupt other native communities.


Subject(s)
Animal Distribution , Conservation of Natural Resources/methods , Falconiformes/physiology , Predatory Behavior/physiology , Snails/physiology , Animals , Biological Evolution , Environmental Monitoring , Florida , Introduced Species , Population Density , Population Growth , Reproduction/physiology , Seasons , Survival Analysis , Time Factors , United States , Wetlands
9.
PLoS One ; 11(9): e0162690, 2016.
Article in English | MEDLINE | ID: mdl-27681854

ABSTRACT

While variation in age structure over time and space has long been considered important for population dynamics and conservation, reliable estimates of such spatio-temporal variation in age structure have been elusive for wild vertebrate populations. This limitation has arisen because of problems of imperfect detection, the potential for temporary emigration impacting assessments of age structure, and limited information on age. However, identifying patterns in age structure is important for making reliable predictions of both short- and long-term dynamics of populations of conservation concern. Using a multistate superpopulation estimator, we estimated region-specific abundance and age structure (the proportion of individuals within each age class) of a highly endangered population of snail kites for two separate regions in Florida over 17 years (1997-2013). We find that in the southern region of the snail kite-a region known to be critical for the long-term persistence of the species-the population has declined significantly since 1997, and during this time, it has increasingly become dominated by older snail kites (> 12 years old). In contrast, in the northern region-a region historically thought to serve primarily as drought refugia-the population has increased significantly since 2007 and age structure is more evenly distributed among age classes. Given that snail kites show senescence at approximately 13 years of age, where individuals suffer higher mortality rates and lower breeding rates, these results reveal an alarming trend for the southern region. Our work illustrates the importance of accounting for spatial structure when assessing changes in abundance and age distribution and the need for monitoring of age structure in imperiled species.

10.
J Anim Ecol ; 85(6): 1563-1573, 2016 Nov.
Article in English | MEDLINE | ID: mdl-27392248

ABSTRACT

Understanding the spatial scale of population structure is fundamental to long-standing tenets of population biology, landscape ecology and conservation. Nonetheless, identifying such scales has been challenging because a key factor that influences scaling - movement among patches or local populations - is a multicausal process with substantial phenotypic and temporal variation. We resolve this problem via a novel application of network modularity. When applied to movements, modularity provides a formal description of the functional aggregation of populations and identifies potentially critical scales for ecological and evolutionary dynamics. We first test for modularity using several different types of biologically relevant movements across the entire geographic range of an endangered bird, the snail kite (Rostrhamus sociabilis plumbeus). We then ask whether variation in movement based on (i) age, (ii) sex and (iii) time (annual, seasonal and within-season movements) influences spatial population structure (i.e. modularity) in snail kites. We identified significant modularity in annual dispersal of snail kites (all adults, males only, females only, and juveniles only) and in within-breeding season movements of adults, yet no evidence of modularity in seasonal (non-breeding) movements. For those movements with observed modular structure, we found striking similarities in the spatial configuration of population structure, even though movement properties varied considerably among these different types of movements. Our results suggest that the emergence of modularity in population networks can be robust despite movement heterogeneity and differences in patch-based measures of connectivity. Furthermore, our comparison of the population structure and connectivity across multiple movement phases helps to identify wetland patches most critical to population connectivity at multiple spatiotemporal scales. We argue that understanding modularity in populations may provide a robust complement to existing measures of population structure and connectivity and will help to clarify the limiting roles of movement for populations. Such information is increasingly needed for interpreting population persistence and guiding effective conservation strategies with ongoing environmental change.


Subject(s)
Animal Distribution , Conservation of Natural Resources , Movement , Raptors/physiology , Age Factors , Animals , Endangered Species , Florida , Population Dynamics , Sex Factors , Time Factors
11.
Genome Announc ; 4(3)2016 May 12.
Article in English | MEDLINE | ID: mdl-27174274

ABSTRACT

Here, we present the complete genome sequences of two Zika virus (ZIKV) strains, Zika virus/Homo sapiens-tc/THA/2014/SV0127-14 and Zika virus/H. sapiens-tc/PHL/2012/CPC-0740, isolated from the blood of patients collected in Thailand, 2014, and the Philippines, 2012, respectively. Sequencing and phylogenetic analysis showed that both strains belong to the Asian lineage.

12.
Proc Biol Sci ; 282(1814)2015 Sep 07.
Article in English | MEDLINE | ID: mdl-26336178

ABSTRACT

Understanding dispersal and habitat selection behaviours is central to many problems in ecology, evolution and conservation. One factor often hypothesized to influence habitat selection by dispersers is the natal environment experienced by juveniles. Nonetheless, evidence for the effect of natal environment on dispersing, wild vertebrates remains limited. Using 18 years of nesting and mark-resight data across an entire North American geographical range of an endangered bird, the snail kite (Rostrhamus sociabilis), we tested for natal effects on breeding-site selection by dispersers and its consequences for reproductive success and population structure. Dispersing snail kites were more likely to nest in wetlands of the same habitat type (lacustrine or palustrine) as their natal wetland, independent of dispersal distance, but this preference declined with age and if individuals were born during droughts. Importantly, dispersing kites that bred in natal-like habitats had lower nest success and productivity than kites that did not. These behaviours help explain recently described population connectivity and spatial structure across their geographical range and reveal that assortative breeding is occurring, where birds are more likely to breed with individuals born in the same wetland type as their natal habitat. Natal environments can thus have long-term and large-scale effects on populations in nature, even in highly mobile animals.


Subject(s)
Acclimatization/physiology , Animal Distribution , Ecosystem , Falconiformes/physiology , Nesting Behavior , Age Factors , Animals , Florida , Geography , Reproduction/physiology , Wetlands
13.
Nat Commun ; 4: 2572, 2013.
Article in English | MEDLINE | ID: mdl-24096937

ABSTRACT

For nearly a century, biologists have emphasized the profound importance of spatial scale for ecology, evolution and conservation. Nonetheless, objectively identifying critical scales has proven incredibly challenging. Here we extend new techniques from physics and social sciences that estimate modularity on networks to identify critical scales for movement and gene flow in animals. Using four species that vary widely in dispersal ability and include both mark-recapture and population genetic data, we identify significant modularity in three species, two of which cannot be explained by geographic distance alone. Importantly, the inclusion of modularity in connectivity and population viability assessments alters conclusions regarding patch importance to connectivity and suggests higher metapopulation viability than when ignoring this hidden spatial scale. We argue that network modularity reveals critical meso-scales that are probably common in populations, providing a powerful means of identifying fundamental scales for biology and for conservation strategies aimed at recovering imperilled species.


Subject(s)
Coleoptera/physiology , Ecosystem , Falconiformes/physiology , Models, Genetic , Rana catesbeiana/physiology , Ursidae/physiology , Animal Distribution , Animals , Biological Evolution , Conservation of Natural Resources , Gene Flow , Population Dynamics
15.
Acad Med ; 87(5): 635-42, 2012 May.
Article in English | MEDLINE | ID: mdl-22450181

ABSTRACT

A growing number of older adults coupled with a limited number of physicians trained in geriatrics presents a major challenge to ensuring quality medical care for this population. Innovations to incorporate geriatrics education into internal medicine residency programs are needed. To meet this need, in 2009, faculty at the Medical University of South Carolina developed Aging Q(3)-Quality Education, Quality Care, and Quality of Life. This multicomponent initiative recognizes the need for improved geriatrics educational tools and faculty development as well as systems changes to improve the knowledge and clinical performance of residents. To achieve these goals, faculty employ multiple intervention strategies, including lectures, rounds, academic detailing, visual cues, and electronic medical record prompts and decision support. The authors present examples from specific projects, based on care areas including vision screening, fall prevention, and caring for patients with dementia, all of which are based on the Assessing Care of Vulnerable Elders quality indicators. The authors describe the principles driving the design, implementation, and evaluation of the Aging Q(3) program. They present data from multiple sources that illustrate the effectiveness of the interventions to meet the knowledge, skill level, and behavior goals. The authors also address major challenges, including the maintenance of the teaching and modeling interventions over time within the context of demanding primary care and inpatient settings. This organized, evidence-based approach to quality improvement in resident education, as well as faculty leadership development, holds promise for successfully incorporating geriatrics education into internal medicine residencies.


Subject(s)
Aging , Clinical Competence/standards , Faculty, Medical/standards , Geriatrics/education , Internal Medicine/education , Internship and Residency/trends , Program Evaluation , Aged , Humans , Internship and Residency/standards , United States
16.
Ecology ; 93(12): 2580-9, 2012 Dec.
Article in English | MEDLINE | ID: mdl-23431589

ABSTRACT

Extreme weather events, such as droughts and heat waves, are expected to become more severe and more frequent in the coming years, and understanding their impacts on demographic rates is of increasing interest to both evolutionary ecologists and conservation practitioners. An individual's breeding probability can be a sensitive indicator of the decision to initiate reproductive behavior under varying environmental conditions, has strong fitness consequences, and can be considered the first step in a life history trade-off between allocating resources for breeding activities or self-survival. Using a 14-year time series spanning large variation in climatic conditions and the entirety of a population's breeding range, we estimated the effects of extreme weather conditions (drought) on the state-specific probabilities of breeding and survival of an endangered bird, the Florida Snail Kite (Rostrhamus sociabilis plumbeus). Our analysis accounted for uncertainty in breeding status assignment, a common source of uncertainty that is often ignored when states are based on field observations. Breeding probabilities in adult kites (> 1 year of age) decreased during droughts, whereas the probability of breeding in young kites (1 year of age) tended to increase. Individuals attempting to breed showed no evidence of reduced future survival. Although population viability analyses of this species and other species often implicitly assume that all adults will attempt to breed, we find that breeding probabilities were significantly < 1 for all 13 estimable years considered. Our results suggest that experience is an important factor determining whether or not individuals attempt to breed during harsh environmental conditions and that reproductive effort may be constrained by an individual's quality and/or despotic behavior among individuals attempting to breed.


Subject(s)
Endangered Species , Falconiformes/physiology , Reproduction/physiology , Sexual Behavior, Animal/physiology , Weather , Animals , Conservation of Natural Resources , Droughts , Models, Biological
17.
Proc Natl Acad Sci U S A ; 108(48): 19282-7, 2011 Nov 29.
Article in English | MEDLINE | ID: mdl-22084081

ABSTRACT

Network analysis is on the rise across scientific disciplines because of its ability to reveal complex, and often emergent, patterns and dynamics. Nonetheless, a growing concern in network analysis is the use of limited data for constructing networks. This concern is strikingly relevant to ecology and conservation biology, where network analysis is used to infer connectivity across landscapes. In this context, movement among patches is the crucial parameter for interpreting connectivity but because of the difficulty of collecting reliable movement data, most network analysis proceeds with only indirect information on movement across landscapes rather than using observed movement to construct networks. Statistical models developed for social networks provide promising alternatives for landscape network construction because they can leverage limited movement information to predict linkages. Using two mark-recapture datasets on individual movement and connectivity across landscapes, we test whether commonly used network constructions for interpreting connectivity can predict actual linkages and network structure, and we contrast these approaches to social network models. We find that currently applied network constructions for assessing connectivity consistently, and substantially, overpredict actual connectivity, resulting in considerable overestimation of metapopulation lifetime. Furthermore, social network models provide accurate predictions of network structure, and can do so with remarkably limited data on movement. Social network models offer a flexible and powerful way for not only understanding the factors influencing connectivity but also for providing more reliable estimates of connectivity and metapopulation persistence in the face of limited data.


Subject(s)
Conservation of Natural Resources/methods , Ecology/methods , Ecosystem , Hemiptera/physiology , Models, Biological , Movement/physiology , Social Support , Animals , Florida , Opuntia
19.
J Am Geriatr Soc ; 59(10): 1941-6, 2011 Oct.
Article in English | MEDLINE | ID: mdl-21883104

ABSTRACT

Falls are a major problem in older adults, and physicians receive inadequate training in falls evaluation. A multicomponent program (lecture, academic detailing, and case studies) was implemented to enhance medical residents' knowledge, skills, decisions, and interventions made about falls as part of a larger project to improve assessment and care of older adults. Electronic medical record (EMR) template modifications provided cues and reminders, decision support, and documentation into the visit note. Nursing staff and the EMR prompted residents to evaluate patients with a history of falls. Knowledge and confidence were assessed using a pre- and postintervention questionnaire, and an attending physician assessed skills by direct observation of the Timed Up and Go Test (TUG). Effect on clinical actions was assessed using the EMR database. Participation in training of faculty and staff was high. Over the 3-month intervention period, an attending physician reviewed the detailing sheet outlining important points of the training with 86% of residents, and 64% demonstrated a TUG to an attending physician. Of 895 older adults seen, 15% (134) had a positive screen for falls, of whom 92% (123) had an EMR falls template completed, and 42% (56) had a TUG performed. Of the patients evaluated with the TUG, 53% (29) failed. A review of charts for patients who failed the screen or TUG revealed that the majority had special circumstances limiting their participation, even after a physical therapy evaluation. Education and system changes facilitated improvements in resident knowledge, skill, self-efficacy, and clinical action in screening, evaluating, and managing falls in older adults.


Subject(s)
Accidental Falls/prevention & control , Cooperative Behavior , Interdisciplinary Communication , Internal Medicine/education , Internship and Residency , Mass Screening , Patient Care Team , Activities of Daily Living/classification , Aged , Algorithms , Clinical Competence , Curriculum , Disability Evaluation , Electronic Health Records , Female , Humans , Male , Postural Balance , Risk Assessment , Southeastern United States , Wounds and Injuries/prevention & control
20.
Osteoarthritis Cartilage ; 18(9): 1159-66, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20633676

ABSTRACT

OBJECTIVE: To develop a short-term in vivo model in rats, with an enzyme-linked immunosorbent assay (ELISA) readout for specific aggrecanase-cleaved aggrecan fragments, to facilitate testing of aggrecanase inhibitors. METHODS: Monosodium iodoacetate (MIA), a metabolic inhibitor, was injected into the right knee joint of male Lewis rats and the release of aggrecanase-cleaved fragments of aggrecan containing the NITEGE or ARGN neoepitope was measured in the synovial fluid at 7 days post MIA injection using novel ELISAs. The ELISAs utilize a commercial antibody directed against the hyaluronic-acid binding region (HABR) of aggrecan, in combination with either an alpha-NITEGE antibody (NITEGE ELISA) or an alpha-ARGS/BC3 antibody (ARGS ELISA), to detect aggrecanase-cleavage of aggrecan within the interglobular domain (IGD). Aggrecan fragments present in in vitro digests, in cytokine-treated cartilage explant culture supernatants and in rat synovial fluid lavage samples were detected and quantified using the two ELISAs. Small molecule inhibitors of aggrecanase activity were dosed orally on days 3-7 to determine their ability to inhibit MIA-induced generation of the NITEGE and ARGN neoepitopes measured in the rat synovial fluid. RESULTS: The NITEGE assay was shown to specifically detect the N-terminal fragment of aggrecan comprising the G1 domain and the NITEGE neoepitope sequence. This assay can readily measure aggrecanase-cleaved bovine, human and rat aggrecan without the need for deglycosylation. The ARGS assay specifically detects C-terminal fragments of aggrecan comprising the ARGS/ARGN neoepitope and the G2 domain. Keratan sulfate (KS) residues of aggrecan interfere with this ELISA, and hence this assay works well with native rat articular cartilage aggrecan (that lacks KS residues) and with deglycosylated bovine and human aggrecan. Injection of MIA into the rat knee joints resulted in a time-dependent increase in the release of aggrecanase-cleaved aggrecan fragments into the synovial fluid and treatment with an aggrecanase inhibitor resulted in a dose-dependent inhibition of the generation of these neoepitopes. CONCLUSIONS: We have established a short-term in vivo model in rats that involves measurement of synovial fluid biomarkers that are dependent on aggrecanase activity in the joint. The short duration of the model combined with the mechanistic biomarker readout makes it very useful for the initial in vivo screening of aggrecanase inhibitors prior to testing them in time and resource-intensive disease models of osteoarthritis (OA).


Subject(s)
Aggrecans/metabolism , Endopeptidases/pharmacokinetics , Iodoacetates/pharmacology , Synovial Fluid/metabolism , Animals , Biomarkers/metabolism , Cattle , Endopeptidases/metabolism , Enzyme-Linked Immunosorbent Assay/methods , Epitopes/analysis , Humans , Knee Joint/metabolism , Male , Rats , Rats, Inbred Lew
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