Subject(s)
Acid Phosphatase/analysis , Arylsulfatases/analysis , Cytoplasmic Granules/enzymology , Esterases/analysis , Keratins , Skin/ultrastructure , Sulfatases/analysis , Animals , Endoplasmic Reticulum/enzymology , Golgi Apparatus/enzymology , Histocytochemistry , Lysosomes/enzymology , Mice , Skin/enzymologySubject(s)
Skin/injuries , Wound Healing , Animals , Blister/pathology , Cell Movement , Disease Models, Animal , Keratins/physiology , Mice , Microtomy , Skin/pathology , Skin/ultrastructureSubject(s)
Antibodies/analysis , Peptidoglycan , Rheumatic Fever/immunology , Rheumatic Heart Disease/immunology , Adult , Animals , Antibodies, Bacterial/analysis , Carbon Radioisotopes , Child , Cross Reactions , Epitopes , Female , Humans , Impetigo/immunology , Male , Micrococcus/immunology , Rabbits/immunology , Streptococcal Infections/immunology , Streptococcus/immunologySubject(s)
Blister/pathology , Desmosomes , Skin/cytology , Wound Healing , Animals , Biopsy , Cell Membrane , Disease Models, Animal , Mice , Microscopy, Electron , Models, Biological , MorphogenesisSubject(s)
Catechol Oxidase/metabolism , Melanoma/enzymology , Tyrosine/metabolism , Animals , Chromatography , Dihydroxyphenylalanine/metabolism , Fumarates/metabolism , Hydrogen-Ion Concentration , Methods , Mice , Monophenol Monooxygenase/metabolism , Neoplasm Transplantation , Neoplasms, Experimental/enzymology , Oxidation-Reduction , Radioisotopes , Spectrophotometry , Time Factors , Tyrosine/analysisSubject(s)
Organoids/analysis , Osmium , Skin/analysis , Animals , Golgi Apparatus , Histological Techniques , Iodine , Lipids/analysis , Lysosomes , Mice , Mitochondria , Skin/cytology , Staining and Labeling , ZincSubject(s)
Pigmentation Disorders/pathology , Skin/pathology , Adolescent , Biopsy , Cytoplasmic Granules , Female , Humans , Microscopy, ElectronSubject(s)
Connective Tissue Cells , Dendrites/cytology , Skin/cytology , Adolescent , Basement Membrane/cytology , Biopsy , Catecholamines/analysis , Cytoplasmic Granules , Endoplasmic Reticulum , Female , Humans , Langerhans Cells/cytology , Melanocytes/cytology , Microscopy, Electron , Nerve Tissue/cytology , Pigmentation Disorders/pathology , Skin/innervation , Skin/pathologySubject(s)
Keratosis/etiology , Sunburn , Ultraviolet Rays/adverse effects , Humans , Keratins , Lysosomes , Microscopy, Electron , Skin/cytology , Skin/pathology , Time FactorsABSTRACT
Fixation of epidermis with a mixture of osmium tetroxide and zinc iodide (OsO(4)-ZnI(2)) for 24 hr renders the central periodic lamella of the Langerhans cell granule (LCG), the Golgi region, and the nuclear envelope of epidermal Langerhans cells preferentially visible. The use of this technique on Langerhans cells in normal epidermis and in epidermis of patients with histiocytosis (Letterer-Siwe disease) allows a broader visualization of the LCG's than was heretofore possible with routine glutaraldehyde-osmium tetroxide fixation and uranyl acetate-lead staining. The identical staining of Golgi apparatus and LCG favors the view that there is close relation between the Golgi area and the LCG's. Different staining characteristics of the LCG's near the Golgi region and at the cell periphery, respectively, may suggest that the LCG undergoes changes on its way from the Golgi area towards the extracellular space. The hypothesis is advanced that the material which is heavily impregnated with metal after fixation with OsO(4)-ZnI(2) might be a lipid.
Subject(s)
Islets of Langerhans/cytology , Skin/pathology , Staining and Labeling , Adult , Biopsy , Cell Nucleus , Cytoplasmic Granules , Golgi Apparatus , Histiocytosis, Langerhans-Cell/pathology , Humans , Infant , Iodides , Lipids , Methods , Microscopy , Microscopy, Electron , Osmium , ZincSubject(s)
Cantharidin/toxicity , Kidney/drug effects , Liver/drug effects , Animals , Cell Membrane , Cholesterol/analysis , Endoplasmic Reticulum , Kidney Glomerulus/pathology , Kidney Tubules/drug effects , Liver/analysis , Male , Microscopy, Electron , Microsomes , Mitochondria, Liver , Phospholipids/analysis , RatsSubject(s)
Cell Biology , Psoriasis/pathology , Cell Membrane , Cytoplasm , Golgi Apparatus , Histocytochemistry , Humans , Male , Microscopy , Microscopy, Electron , Middle Aged , Polysaccharides/analysisABSTRACT
Tyrosinase inhibitor (molecular weight less than 5000; extracted from various melanomas) fully inhibits soluble tyrosinase but only partially inhibits tyrosinase "aggregated" into melanosomes; the inhibitor can be inactivated by ultraviolet light. S91 Albinotyrosinase Type B apparently cannot "aggregate" into melanosomes because its protein carrier is genetically altered. Therefore, albinotyrosinase remains vulnerable to its inhibitor and cannot produce melanin, even though the enzyme has a functioning active center.
