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PLoS One ; 6(2): e14732, 2011 Feb 25.
Article in English | MEDLINE | ID: mdl-21364913

ABSTRACT

BACKGROUND: Vasculogenesis, the de novo formation of blood vessels from precursor cells is critical for a developing embryo. However, the signals and events that dictate the formation of primary axial vessels remain poorly understood. METHODOLOGY/PRINCIPAL FINDINGS: In this study, we use ets-related protein-1 (etsrp), which is essential for vascular development, to analyze the early stages of vasculogenesis in zebrafish. We found etsrp(+) cells of the head, trunk and tail follow distinct developmental sequences. Using a combination of genetic, molecular and chemical approaches, we demonstrate that fli(+)etsrp(+) hemato-vascular progenitors (FEVPs) are proliferating at the lateral plate mesoderm (LPM). The Shh-VEGF-Notch-Hey2 signaling pathway controls the proliferation process, and experimental modulation of single components of this pathway alters etsrp(+) cell numbers at the LPM. CONCLUSIONS/SIGNIFICANCE: This study for the first time defines factors controlling proliferation, and cell numbers of pre-migratory FEVPs in zebrafish.


Subject(s)
Blood Vessels/embryology , Cell Proliferation , Hematopoietic Stem Cells/physiology , Mesoderm/cytology , Proto-Oncogene Protein c-fli-1/metabolism , Zebrafish Proteins/metabolism , Zebrafish/embryology , Animals , Blood Vessels/metabolism , Cell Movement/physiology , Embryo, Nonmammalian , Endothelium, Vascular/embryology , Endothelium, Vascular/metabolism , Endothelium, Vascular/physiology , Gene Expression Regulation, Developmental , Head/embryology , Hematopoietic Stem Cells/metabolism , Mesoderm/metabolism , Mesoderm/physiology , Models, Biological , Neovascularization, Physiologic/genetics , Neovascularization, Physiologic/physiology , Proto-Oncogene Protein c-fli-1/genetics , Proto-Oncogene Protein c-fli-1/physiology , Tail/embryology , Zebrafish/genetics , Zebrafish/metabolism , Zebrafish/physiology , Zebrafish Proteins/genetics , Zebrafish Proteins/physiology
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