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1.
PLoS One ; 18(7): e0288162, 2023.
Article in English | MEDLINE | ID: mdl-37418424

ABSTRACT

A history of infection has been linked with increased risk of acute myeloid leukaemia (AML) and related myelodysplastic syndromes (MDS). Furthermore, AML and MDS patients suffer frequent infections because of disease-related impaired immunity. However, the role of infections in the development and progression of AML and MDS remains poorly understood. We and others previously demonstrated that the human nucleoside diphosphate kinase (NDPK) NM23-H1 protein promotes AML blast cell survival by inducing secretion of IL-1ß from accessory cells. NDPKs are an evolutionary highly conserved protein family and pathogenic bacteria secrete NDPKs that regulate virulence and host-pathogen interactions. Here, we demonstrate the presence of IgM antibodies against a broad range of pathogen NDPKs and more selective IgG antibody activity against pathogen NDPKs in the blood of AML patients and normal donors, demonstrating that in vivo exposure to NDPKs likely occurs. We also show that pathogen derived NDPK-proteins faithfully mimic the catalytically independent pro-survival activity of NM23-H1 against primary AML cells. Flow cytometry identified that pathogen and human NDPKs selectively bind to monocytes in peripheral blood. We therefore used vitamin D3 differentiated monocytes from wild type and genetically modified THP1 cells as a model to demonstrate that NDPK-mediated IL-1ß secretion by monocytes is NLRP3-inflammasome and caspase 1 dependent, but independent of TLR4 signaling. Monocyte stimulation by NDPKs also resulted in activation of NF-κB and IRF pathways but did not include the formation of pyroptosomes or result in pyroptotic cell death which are pivotal features of canonical NLRP3 inflammasome activation. In the context of the growing importance of the NLRP3 inflammasome and IL-1ß in AML and MDS, our findings now implicate pathogen NDPKs in the pathogenesis of these diseases.


Subject(s)
Monocytes , Nucleoside-Diphosphate Kinase , Humans , Monocytes/metabolism , Inflammasomes/metabolism , Nucleoside-Diphosphate Kinase/metabolism , NLR Family, Pyrin Domain-Containing 3 Protein/metabolism , Cell Survival , Interleukin-1beta/metabolism
4.
Nature ; 574(7777): 273-277, 2019 10.
Article in English | MEDLINE | ID: mdl-31578525

ABSTRACT

Transcription and pre-mRNA splicing are key steps in the control of gene expression and mutations in genes regulating each of these processes are common in leukaemia1,2. Despite the frequent overlap of mutations affecting epigenetic regulation and splicing in leukaemia, how these processes influence one another to promote leukaemogenesis is not understood and, to our knowledge, there is no functional evidence that mutations in RNA splicing factors initiate leukaemia. Here, through analyses of transcriptomes from 982 patients with acute myeloid leukaemia, we identified frequent overlap of mutations in IDH2 and SRSF2 that together promote leukaemogenesis through coordinated effects on the epigenome and RNA splicing. Whereas mutations in either IDH2 or SRSF2 imparted distinct splicing changes, co-expression of mutant IDH2 altered the splicing effects of mutant SRSF2 and resulted in more profound splicing changes than either mutation alone. Consistent with this, co-expression of mutant IDH2 and SRSF2 resulted in lethal myelodysplasia with proliferative features in vivo and enhanced self-renewal in a manner not observed with either mutation alone. IDH2 and SRSF2 double-mutant cells exhibited aberrant splicing and reduced expression of INTS3, a member of the integrator complex3, concordant with increased stalling of RNA polymerase II (RNAPII). Aberrant INTS3 splicing contributed to leukaemogenesis in concert with mutant IDH2 and was dependent on mutant SRSF2 binding to cis elements in INTS3 mRNA and increased DNA methylation of INTS3. These data identify a pathogenic crosstalk between altered epigenetic state and splicing in a subset of leukaemias, provide functional evidence that mutations in splicing factors drive myeloid malignancy development, and identify spliceosomal changes as a mediator of IDH2-mutant leukaemogenesis.


Subject(s)
Alternative Splicing/genetics , Carcinogenesis/genetics , Epigenesis, Genetic , Leukemia, Myeloid, Acute/genetics , Animals , Cell Line, Tumor , Cell Proliferation , DNA Methylation , DNA-Binding Proteins/genetics , Female , Gene Expression Regulation, Neoplastic , Humans , Isocitrate Dehydrogenase/genetics , Male , Mutation/genetics , RNA Polymerase II/metabolism , Serine-Arginine Splicing Factors/genetics , Transcriptome
7.
Genes Dev ; 20(17): 2421-36, 2006 Sep 01.
Article in English | MEDLINE | ID: mdl-16951255

ABSTRACT

The Schizosaccharomyces pombe CLIP170-associated protein (CLASP) Peg1 was identified in a screen for mutants with spindle formation defects and a screen for molecules that antagonized EB1 function. The conditional peg1.1 mutant enabled us to identify key features of Peg1 function. First, Peg1 was required to form a spindle and astral microtubules, yet destabilized interphase microtubules. Second, Peg1 was required to slow the polymerization rate of interphase microtubules that establish end-on contact with the cortex at cell tips. Third, Peg1 antagonized the action of S. pombe CLIP170 (Tip1) and EB1 (Mal3). Fourth, although Peg1 resembled higher eukaryotic CLASPs by physically associating with both Mal3 and Tip1, neither Tip1 nor Mal3 was required for Peg1 to destabilize interphase microtubules or for it to associate with microtubules. Conversely, neither Mal3 nor Tip1 required Peg1 to associate with microtubules or cell tips. Consistently, while mal3.Delta and tip1.Delta disrupted linear growth, corrupting peg1 (+) did not. Fifth, peg1.1 phenotypes resembled those arising from deletion of the single heavy or both light chains of fission yeast dynein. Furthermore, all interphase phenotypes arising from peg1 (+) manipulation relied on dynein function. Thus, the impact of S. pombe CLASP on interphase microtubule behavior is more closely aligned to dynein than EB1 or CLIP170.


Subject(s)
Dyneins/physiology , Microtubule-Associated Proteins , Microtubules/metabolism , Neoplasm Proteins , Nuclear Proteins/metabolism , Schizosaccharomyces pombe Proteins/metabolism , Schizosaccharomyces/metabolism , Interphase/physiology , Microtubule-Associated Proteins/physiology , Mitosis/physiology , Neoplasm Proteins/physiology , Nuclear Proteins/physiology , Schizosaccharomyces/cytology , Schizosaccharomyces/physiology , Schizosaccharomyces pombe Proteins/physiology , Spindle Apparatus/metabolism
8.
Oncogene ; 21(53): 8067-74, 2002 Nov 21.
Article in English | MEDLINE | ID: mdl-12444543

ABSTRACT

Deregulation of D-type cyclin-dependent kinases (CDK4 and 6) is widely observed in various human cancers, illustrating their importance in cell cycle control. Like other cyclin-dependent kinases (CDKs), assembly with cyclins is the most critical step for activation of CDK4/6. As previously reported elsewhere, we observed that the level of cyclinD1-CDK4 complex and its associated kinase activity were significantly low in asynchronously proliferating mouse embryo fibroblasts lacking both p21(Cip1) and p27(Kip1) (p21/p27-null MEFs). These evidences imply that p21(Cip1) and p27(Kip1) CDK inhibitors are 'essential activators' of cyclin D-kinases. We, however, discovered here that both the assembly and activation of cyclin D1-CDK4 complex occur when quiescent p21/p27-null MEFs were stimulated to re-enter the cell cycle. This mitogen-induced cyclin D1-kinase activity was blocked by overexpression of p16(INK4a) and resulted in the inhibition of S phase entry in p21/p27-null MEFs. Furthermore, ectopic expression of p34(SEI-1), a mitogen-induced CDK4 binding protein, increased the levels of active cyclinD1-CDK4 complex in asynchronously proliferating p21/p27-null MEFs. Together, our results suggest that there are several independent ways to stimulate the assembly of cyclin D1-CDK4 kinases. Although p21(Cip1) and p27(Kip1) play a role in this process, our results demonstrate that additional mechanisms must occur in G0 to S phase transition.


Subject(s)
Cell Cycle/physiology , Cyclin-Dependent Kinases/metabolism , Cyclins/deficiency , Fibroblasts/metabolism , Nuclear Proteins , Proto-Oncogene Proteins , Tumor Suppressor Proteins/deficiency , Animals , Cattle , Cell Cycle/drug effects , Cell Cycle Proteins/physiology , Contact Inhibition , Culture Media/pharmacology , Culture Media, Serum-Free , Cyclin-Dependent Kinase 4 , Cyclin-Dependent Kinase Inhibitor p21 , Cyclin-Dependent Kinase Inhibitor p27 , Cyclins/physiology , Embryo, Mammalian/cytology , Enzyme Activation/drug effects , Fetal Blood/physiology , Fibroblasts/cytology , Fibroblasts/drug effects , Gene Targeting , Growth Substances/pharmacology , Humans , Macromolecular Substances , Mice , Mitogens/pharmacology , Resting Phase, Cell Cycle , S Phase , Trans-Activators/physiology , Transcription Factors , Tumor Suppressor Proteins/physiology
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