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1.
EMBO J ; 20(19): 5470-9, 2001 Oct 01.
Article in English | MEDLINE | ID: mdl-11574479

ABSTRACT

U7 snRNPs were isolated from HeLa cells by biochemical fractionation, followed by affinity purification with a biotinylated oligonucleotide complementary to U7 snRNA. Purified U7 snRNPs lack the Sm proteins D1 and D2, but contain additional polypeptides of 14, 50 and 70 kDa. Microsequencing identified the 14 kDa polypeptide as a new Sm-like protein related to Sm D1 and D3. Like U7 snRNA, this protein, named Lsm10, is enriched in Cajal bodies of the cell nucleus. Its incorporation into U7 snRNPs is largely dictated by the special Sm binding site of U7 snRNA. This novel type of Sm complex, composed of both conventional Sm proteins and the Sm-like Lsm10, is most likely to be important for U7 snRNP function and subcellular localization.


Subject(s)
Ribonucleoprotein, U7 Small Nuclear/chemistry , Ribonucleoproteins, Small Nuclear/analysis , Amino Acid Sequence , Animals , Autoantigens , HeLa Cells , Humans , Mice , Models, Molecular , Molecular Sequence Data , Nuclear Proteins/isolation & purification , Peptides , Protein Binding , RNA Processing, Post-Transcriptional , RNA, Small Nuclear/metabolism , Sequence Homology, Amino Acid , snRNP Core Proteins
2.
EMBO J ; 20(16): 4536-46, 2001 Aug 15.
Article in English | MEDLINE | ID: mdl-11500380

ABSTRACT

Previous UV cross-linking studies demonstrated that, upon integration of the U2 snRNP into the spliceosome, a 14 kDa protein (p14) interacts directly with the branch adenosine, the nucleophile for the first transesterification step of splicing. We have identified the cDNA encoding this protein by microsequencing a 14 kDa protein isolated from U2-type spliceosomes. This protein contains an RNA recognition motif and is highly conserved across species. Antibodies raised against this cDNA-encoded protein precipitated the 14 kDa protein cross-linked to the branch adenosine, confirming the identity of the p14 cDNA. A combination of immunoblotting, protein microsequencing and immunoprecipitation revealed that p14 is a component of both 17S U2 and 18S U11/U12 snRNPs, suggesting that it contributes to the interaction of these snRNPs with the branch sites of U2- and U12-type pre-mRNAs, respectively. p14 was also shown to be a subunit of the heteromeric splicing factor SF3b and to interact directly with SF3b155. Immuno precipitations indicated that p14 is present in U12-type spliceosomes, consistent with the idea that branch point selection is similar in the major and minor spliceosomes.


Subject(s)
Phosphoproteins/metabolism , RNA Precursors/metabolism , RNA Splicing , RNA-Binding Proteins/metabolism , Ribonucleoprotein, U2 Small Nuclear/metabolism , Ribonucleoproteins, Small Nuclear/metabolism , Amino Acid Sequence , Animals , Binding Sites , Cloning, Molecular , Conserved Sequence , Cross-Linking Reagents , Evolution, Molecular , HeLa Cells , Humans , Molecular Sequence Data , Peptides/classification , Peptides/genetics , Peptides/metabolism , RNA Splicing Factors , RNA-Binding Proteins/classification , RNA-Binding Proteins/genetics , Ribonucleoprotein, U2 Small Nuclear/classification , Ribonucleoprotein, U2 Small Nuclear/genetics , Ribonucleoproteins, Small Nuclear/classification , Ribonucleoproteins, Small Nuclear/genetics , Sequence Homology, Amino Acid , Spliceosomes/metabolism
3.
Curr Opin Cell Biol ; 13(3): 290-301, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11343899

ABSTRACT

Significant advances have been made in elucidating the biogenesis pathway and three-dimensional structure of the UsnRNPs, the building blocks of the spliceosome. U2 and U4/U6*U5 tri-snRNPs functionally associate with the pre-mRNA at an earlier stage of spliceosome assembly than previously thought, and additional evidence supporting UsnRNA-mediated catalysis of pre-mRNA splicing has been presented.


Subject(s)
RNA, Small Nuclear/metabolism , Ribonucleoprotein, U1 Small Nuclear/biosynthesis , Ribonucleoproteins, Small Nuclear/biosynthesis , Spliceosomes/genetics , Spliceosomes/metabolism , Animals , Crystallography , Humans , Macromolecular Substances , Protein Structure, Tertiary/physiology , Protein Transport/physiology , RNA Splicing/genetics , RNA Splicing/physiology , RNA, Small Nuclear/chemistry , RNA, Small Nuclear/genetics , Ribonucleoprotein, U1 Small Nuclear/chemistry , Ribonucleoprotein, U1 Small Nuclear/genetics , Ribonucleoproteins, Small Nuclear/chemistry , Ribonucleoproteins, Small Nuclear/genetics , Spliceosomes/chemistry
5.
Science ; 284(5422): 2003-5, 1999 Jun 18.
Article in English | MEDLINE | ID: mdl-10373121

ABSTRACT

In metazoans, two distinct spliceosomes catalyzing pre-messenger RNA splicing have been identified. Here, the human U11/U12 small nuclear ribonucleoprotein (snRNP), a subunit of the minor (U12-dependent) spliceosome, was isolated. Twenty U11/U12 proteins were identified, including subsets unique to the minor spliceosome or common to both spliceosomes. Common proteins include four U2 snRNP polypeptides that constitute the essential splicing factor SF3b. A 35-kilodalton U11-associated protein homologous to the U1 snRNP 70K protein was also identified. These data provide fundamental information about proteins of the minor spliceosome and shed light on its evolutionary relationship to the major spliceosome.


Subject(s)
Ribonucleoprotein, U1 Small Nuclear/analysis , Ribonucleoprotein, U2 Small Nuclear/analysis , Ribonucleoproteins, Small Nuclear/analysis , Spliceosomes/chemistry , Amino Acid Sequence , Chromatography, Affinity , Evolution, Molecular , HeLa Cells , Humans , Introns , Molecular Sequence Data , Molecular Weight , RNA Splicing , Ribonucleoprotein, U1 Small Nuclear/isolation & purification , Ribonucleoprotein, U2 Small Nuclear/isolation & purification , Ribonucleoproteins, Small Nuclear/isolation & purification
6.
Mol Cell Biol ; 19(4): 2782-90, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10082544

ABSTRACT

The function of conserved regions of the metazoan U5 snRNA was investigated by reconstituting U5 small nuclear ribonucleoprotein particles (snRNPs) from purified snRNP proteins and HeLa or Xenopus U5 snRNA mutants and testing their ability to restore splicing to U5-depleted nuclear extracts. Substitution of conserved nucleotides comprising internal loop 2 or deletion of internal loop 1 had no significant effect on the ability of reconstituted U5 snRNPs to complement splicing. However, deletion of internal loop 2 abolished U5 activity in splicing and spliceosome formation. Surprisingly, substitution of the invariant loop 1 nucleotides with a GAGA tetraloop had no effect on U5 activity. Furthermore, U5 snRNPs reconstituted from an RNA formed by annealing the 5' and 3' halves of the U5 snRNA, which lacked all loop 1 nucleotides, complemented both steps of splicing. Thus, in contrast to yeast, loop 1 of the human U5 snRNA is dispensable for both steps of splicing in HeLa nuclear extracts. This suggests that its function can be compensated for in vitro by other spliceosomal components: for example, by proteins associated with the U5 snRNP. Consistent with this idea, immunoprecipitation studies indicated that several functionally important U5 proteins associate stably with U5 snRNPs containing a GAGA loop 1 substitution.


Subject(s)
Conserved Sequence , RNA Splicing , RNA, Small Nuclear/genetics , Ribonucleoprotein, U5 Small Nuclear/metabolism , Animals , Base Sequence , Cell Nucleus , Cell-Free System , HeLa Cells , Humans , Mutation , Nucleic Acid Conformation , RNA Precursors/metabolism , RNA, Messenger/metabolism , RNA, Small Nuclear/chemistry , Xenopus
7.
RNA ; 3(11): 1313-26, 1997 Nov.
Article in English | MEDLINE | ID: mdl-9409622

ABSTRACT

Through UV-crosslinking experiments, we previously provided evidence suggesting that a U5 snRNP protein with a molecular weight in the 100-kDa range is an ATP-binding protein (Laggerbauer B, Lauber J, Lührmann R, 1996, Nucleic Acid Res 24:868-875). Separation of HeLa U5 snRNP proteins on 2D gels revealed multiple variants with apparent molecular masses of 100 kDa. Subsequent microsequencing of these variants led to the isolation of a cDNA encoding a protein with an N-terminal RS domain and a C-terminal domain that contains all of the conserved motifs characteristic of members of the DEAD-box family of RNA-stimulated ATPases and RNA helicases. Antibodies raised against cDNA-encoded 100-kDa protein specifically recognized native U5-100kD both on immunoblots and in purified HeLa U5 snRNPs or [U4/U6.U5] tri-snRNP complexes, confirming that the bona fide 100-kDa cDNA had been isolated. In vitro phosphorylation studies demonstrated that U5-100kD can serve as a substrate for both Clk/Sty and the U1 snRNP-associated kinase, and further suggested that the multiple U5-100kD variants observed on 2D gels represent differentially phosphorylated forms of the protein. A database homology search revealed a significant degree of homology (60% similarity, 37% identity) between the Saccharomyces cerevisiae splicing factor, Prp28p, which lacks an N-terminal RS domain, and the C-terminal domain of U5-100kD. Consistent with their designation as structural homologues, anti-Prp28 antibodies recognized specifically the human U5-100kD protein on immunoblots. Together with the DEXH-box U5-200kD protein (Lauber J et al., 1996, EMBO J 15:4001-4015), U5-100kD is the second example of a putative RNA helicase that is tightly associated with the U5 snRNP. Given the recent identification of the U5-116kD protein as a homologue of the ribosomal translocase EF-2 (Fabrizio P, Laggerbauer B, Lauber J, Lane WS, Lührmann R, 1997, EMBO J 16:4092-4106), at least three integral U5 snRNP proteins thus potentially facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing.


Subject(s)
RNA Nucleotidyltransferases/genetics , Ribonucleoprotein, U5 Small Nuclear/metabolism , Saccharomyces cerevisiae/genetics , Amino Acid Sequence , DEAD-box RNA Helicases , Electrophoresis, Gel, Two-Dimensional , Humans , Molecular Sequence Data , Molecular Weight , Phosphorylation , Protein Binding , RNA Helicases , RNA Nucleotidyltransferases/chemistry , RNA Nucleotidyltransferases/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Saccharomyces cerevisiae Proteins , Sequence Homology, Amino Acid
8.
RNA ; 3(8): 926-41, 1997 Aug.
Article in English | MEDLINE | ID: mdl-9257651

ABSTRACT

Immunoaffinity-purified human 25S [U4/U6.U5] tri-snRNPs harbor a set of polypeptides, termed the tri-snRNP proteins, that are not present in Mono Q-purified 20S U5 snRNPs or 10S U4/U6 snRNPs and that are important for tri-snRNP complex formation (Behrens SE, Lührmann R, 1991, Genes & Dev 5:1439-1452). Biochemical and immunological characterization of HeLa [U4/U6.U5] tri-snRNPs led to the identification of two novel proteins with molecular weights of 61 and 63kD that are distinct from the previously described 15.5, 20, 27, 60, and 90kD tri-snRNP proteins. For the initial characterization of tri-snRNP proteins that interact directly with U4/U6 snRNPs, immunoaffinity chromatography with an antibody directed against the 60kD protein was performed. We demonstrate that the 60 and 90kD tri-snRNP proteins specifically associate with the U4/U6 snRNP at salt concentrations where the tri-snRNP complex has dissociated. The primary structures of the 60kD and 90kD proteins were determined by cloning and sequencing their respective cDNAs. The U4/U6-60kD protein possesses a C-terminal WD domain that contains seven WD repeats and thus belongs to the WD-protein family, whose best-characterized members include the Gbeta subunits of heterotrimeric G proteins. A database homology search revealed a significant degree of overall homology (57.8% similarity, 33.9% identity) between the human 60kD protein and the Saccharomyces cerevisiae U4/U6 snRNP protein Prp4p. Two additional, previously undetected WD repeats (with seven in total) were also identified in Prp4p, consistent with the possibility that 60kD/Prp4p, like beta-transducin, may adopt a propeller-like structure. The U4/U6-90kD protein was shown to exhibit significant homology, particularly in its C-terminal half, with the S. cerevisiae splicing factor Prp3p, which also associates with the yeast U4/U6 snRNP. Interestingly, U4/U6-90kD shares short regions of homology with E. coli RNase III, including a region encompassing its double-stranded RNA binding domain. Based on their structural similarity with essential splicing factors in yeast, the human U4/U6-60kD and 90kD proteins are likely also to play important roles in the mammalian splicing process.


Subject(s)
Protein Serine-Threonine Kinases/chemistry , Ribonucleoprotein, U4-U6 Small Nuclear/immunology , Saccharomyces cerevisiae Proteins , Amino Acid Sequence , Antibodies , Base Sequence , Chromatography, Affinity/methods , Cloning, Molecular , DNA, Complementary/isolation & purification , Electrophoresis/methods , HeLa Cells , Humans , Molecular Sequence Data , Molecular Weight , Nuclear Proteins/chemistry , Peptides , RNA Splicing Factors , Ribonucleoprotein, U4-U6 Small Nuclear/chemistry , Ribonucleoprotein, U4-U6 Small Nuclear/genetics , Saccharomyces cerevisiae/chemistry , Sequence Analysis , Sequence Homology, Amino Acid
9.
Curr Opin Cell Biol ; 9(3): 320-8, 1997 Jun.
Article in English | MEDLINE | ID: mdl-9159080

ABSTRACT

Proteins have been implicated in an expanding variety of functions during pre-mRNA splicing. Molecular cloning has identified genes encoding spliceosomal proteins that potentially act as novel RNA helicases, GTPases, or protein isomerases. Novel protein-protein and protein-RNA interactions that are required for functional spliceosome formation have also been described. Finally, growing evidence suggests that proteins may contribute directly to the spliceosome's active sites.


Subject(s)
RNA Precursors/metabolism , RNA Splicing , Ribonucleoproteins, Small Nuclear/physiology , Spliceosomes/physiology , Fungal Proteins/metabolism , Macromolecular Substances , Protein Binding , RNA, Fungal/metabolism , RNA, Messenger/metabolism , Ribonucleoproteins/physiology , Saccharomyces cerevisiae
10.
RNA ; 3(4): 344-55, 1997 Apr.
Article in English | MEDLINE | ID: mdl-9085842

ABSTRACT

SR proteins play important roles in the recognition and selection of the 3' and 5' splice site of a given intron and contribute to the phosphorylation/dephosphorylation-mediated regulation of pre-mRNA splicing. Recent studies have demonstrated that the U1 snRNP is recruited to the 5' splice site by protein/protein interactions involving the SR domains of the U1-70K protein and SF2/ASF. Recently, it was suggested that SR proteins might also contribute to the binding of the [U4/U6.U5] tri-snRNP to the pre-spliceosome (Roscigno RF, Garcia-Blanco MA, 1995, RNA 1:692-706), although it remains unclear whether these SR proteins interact with proteins of the tri-snRNP complex. As a first step toward the identification of proteins that could potentially mediate the integration of the [U4/U6.U5] tri-snRNP complex into the spliceosome, we investigated whether purified [U4/U6.U5] tri-snRNP complexes contain SR proteins. Three proteins in the tri-snRNP complex with approximate molecular weights of 27, 60, and 100 kDa were phosphorylated by purified snRNP-associated protein kinase, which has been shown previously to phosphorylate the serine/ arginine-rich domains of U1-70K and SF2/ASF (Woppmann A et al., 1993, Nucleic Acids Res 21:2815-2822). These proteins are thus prime candidates for novel tri-snRNP SR proteins. Here, we describe the biochemical and molecular characterization of the 27K protein. Analysis of a cDNA encoding the 27K protein revealed an N-terminal SR domain strongly homologous (54% identity) to the SR domain of the U1 snRNP-specific 70K protein. In contrast to many other SR proteins, the 27K protein does not contain an RNA-binding domain. The 27K protein can be phosphorylated in vitro by the snRNP-associated protein kinase and exhibits several isoelectric variants upon 2D gel electrophoresis. Thus, the tri-snRNP-specific 27K protein could potentially be involved in SR protein-mediated protein/protein interactions and, additionally, its phosphorylation state could modulate pre-mRNA splicing.


Subject(s)
Fungal Proteins/genetics , Protein Kinases/metabolism , Ribonucleases , Ribonucleoprotein, U4-U6 Small Nuclear/metabolism , Ribonucleoproteins, Small Nuclear/genetics , Ribonucleoproteins, Small Nuclear/metabolism , Ribonucleoproteins , Spliceosomes/metabolism , Amino Acid Sequence , Base Sequence , DNA, Complementary/genetics , Electrophoresis, Gel, Two-Dimensional , HeLa Cells , Humans , Molecular Sequence Data , Phosphorylation , Protein Binding , RNA Splicing , Ribonucleoproteins, Small Nuclear/immunology , Sequence Analysis, DNA , Sequence Homology, Amino Acid
11.
Nucleic Acids Res ; 24(23): 4614-23, 1996 Dec 01.
Article in English | MEDLINE | ID: mdl-8972845

ABSTRACT

We have established an in vitro reconstitution/splicing complementation system which has allowed the investigation of the role of mammalian U1 snRNP components both in splicing and at the early stages of spliceosome formation. U1 snRNPs reconstituted from purified, native snRNP proteins and either authentic or in vitro transcribed U1 snRNA restored both early (E) splicing complex formation and splicing-activity to U1-depleted extracts. In vitro reconstituted U1 snRNPs possessing an m3G or ApppG cap were equally active in splicing, demonstrating that a physiological cap structure is not absolutely required for U1 function. However, the presence of an m7GpppG or GpppG cap was deleterious to splicing, most likely due to competition for the m7G cap binding proteins. No significant reduction in splicing or E complex formation was detected with U1 snRNPs reconstituted from U1 snRNA lacking the RNA binding sites of the U1-70K or U1-A protein (i.e., stem-loop I and II, respectively). Complementation studies with purified HeLa U1 snRNPs lacking subsets of the U1-specific proteins demonstrated a role for the U1-C, but not U1-A, protein in the formation and/or stabilization of early splicing complexes. Studies with recombinant U1-C protein mutants indicated that the N-terminal domain of U1-C is necessary and sufficient for the stimulation of E complex formation.


Subject(s)
RNA Splicing , Ribonucleoprotein, U1 Small Nuclear/metabolism , Spliceosomes/metabolism , Animals , Binding Sites , Binding, Competitive , HeLa Cells , Humans , Mutation , RNA/chemistry , RNA/metabolism , RNA Caps , Recombinant Proteins , Ribonucleoprotein, U1 Small Nuclear/chemistry , Structure-Activity Relationship , Xenopus
12.
EMBO J ; 14(16): 4010-21, 1995 Aug 15.
Article in English | MEDLINE | ID: mdl-7664740

ABSTRACT

An in vitro reconstitution/splicing complementation system has been developed which has allowed the investigation of the role of mammalian U2 and U5 snRNP components in splicing. U2 or U5 snRNP cores are first reconstituted from purified native snRNP core proteins and snRNA in the absence of cellular extract and are subsequently added to splicing extracts depleted of either U2 or U5 snRNP. When snRNPs reconstituted with HeLa U2 or U5 snRNA were added to U2- or U5-depleted nuclear extract, splicing was complemented. Addition of naked snRNA, on the other hand, did not restore splicing, demonstrating that the core proteins are essential for both U2 and U5 snRNP functions in splicing. Hybrid U2 or U5 snRNPs, reconstituted with core proteins isolated from U1 or U2 snRNPs, were equally active in splicing complementation, indicating that the snRNP core proteins are functionally interchangeable. U5 snRNPs reconstituted from in vitro transcribed U5 snRNA restored splicing to a level identical to that observed with particles reconstituted from authentic HeLa U5 snRNA. In contrast, splicing could not be restored to U2-depleted extract by the addition of snRNPs reconstituted from synthetic U2 snRNA, suggesting that U2 snRNA base modifications are essential for U2 snRNP function.


Subject(s)
RNA Splicing/physiology , Ribonucleoprotein, U2 Small Nuclear/metabolism , Ribonucleoprotein, U5 Small Nuclear/metabolism , Ribonucleoproteins, Small Nuclear/metabolism , Base Sequence , Cell Extracts , HeLa Cells , Humans , Molecular Sequence Data , Oligonucleotides, Antisense , Pseudouridine/analysis , RNA Caps/physiology , RNA, Messenger/genetics , RNA, Small Nuclear/isolation & purification , RNA, Small Nuclear/metabolism , Ribonucleoprotein, U2 Small Nuclear/isolation & purification , Ribonucleoprotein, U5 Small Nuclear/isolation & purification , Ribonucleoproteins, Small Nuclear/isolation & purification , Spliceosomes/metabolism
13.
Science ; 267(5204): 1616-7, 1995 Mar 17.
Article in English | MEDLINE | ID: mdl-17808161
14.
EMBO J ; 13(17): 4113-25, 1994 Sep 01.
Article in English | MEDLINE | ID: mdl-8076607

ABSTRACT

The U1 small nuclear ribonucleoprotein particle (snRNP)-specific 70K and A proteins are known to bind directly to stem-loops of the U1 snRNA, whereas the U1-C protein does not bind to naked U1 snRNA, but depends on other U1 snRNP protein components for its association. Focusing on the U1-70K and U1-C proteins, protein-protein interactions contributing to the association of these particle-specific proteins with the U1 snRNP were studied. Immunoprecipitation of complexes formed after incubation of naked U1 snRNA or purified U1 snRNPs lacking their specific proteins (core U1 snRNP) with in vitro translated U1-C protein, revealed that both common snRNP proteins and the U1-70K protein are required for the association of U1-C with the U1 snRNP. Binding studies with various in vitro translated U1-70K mutants demonstrated that the U1-70K N-terminal domain is necessary and sufficient for the interaction of U1-C with core U1 snRNPs. Surprisingly, several N-terminal fragments of the U1-70K protein, which lacked the U1-70K RNP-80 motif and did not bind naked U1 RNA, associated stably with core U1 snRNPs. This suggests that a new U1-70K binding site is generated upon association of common U1 snRNP proteins with U1 RNA. The interaction between the N-terminal domain of U1-70K and the core RNP domain was specific for the U1 snRNP; stable binding was not observed with core U2 or U5 snRNPs, suggesting essential structural differences among snRNP core domains. Evidence for direct protein-protein interactions between U1-specific proteins and common snRNP proteins was supported by chemical crosslinking experiments using purified U1 snRNPs. Individual crosslinks between the U1-70K and the common D2 or B'/B protein, as well as between U1-C and B'/B, were detected. A model for the assembly of U1 snRNP is presented in which the complex of common proteins on the RNA backbone functions as a platform for the association of the U1-specific proteins.


Subject(s)
Autoantigens/metabolism , RNA, Small Nuclear/metabolism , Ribonucleoprotein, U1 Small Nuclear/metabolism , Ribonucleoproteins, Small Nuclear , Binding Sites , Cross-Linking Reagents , DNA Mutational Analysis , Humans , Models, Biological , Precipitin Tests , Protein Binding , RNA-Binding Proteins/metabolism , Ribonucleoprotein, U1 Small Nuclear/ultrastructure , Sequence Deletion , Structure-Activity Relationship , snRNP Core Proteins
15.
Nature ; 368(6467): 119-24, 1994 Mar 10.
Article in English | MEDLINE | ID: mdl-8139654

ABSTRACT

Exactly how specific splice sites are recognized during the processing of complex precursor messenger RNAs is not clear. Small nuclear ribonucleoprotein particles (snRNPs) are involved, but are not sufficient by themselves to define splice sites. Now a human protein essential for splicing in vitro, called alternative splicing factor/splicing factor 2, is shown to cooperate with the U1 snRNP particle in binding pre-mRNA. This cooperation is probably achieved by specific interactions between the arginine/serine-rich domain of the splicing factor and a similar region in a U1 snRNP-specific protein.


Subject(s)
Nuclear Proteins/metabolism , RNA Precursors/metabolism , RNA Splicing , RNA-Binding Proteins/metabolism , Adenoviridae/genetics , Arginine/metabolism , HeLa Cells , Humans , Protein Binding , RNA, Viral/metabolism , Recombinant Proteins/metabolism , Ribonucleoprotein, U1 Small Nuclear/metabolism , Serine/metabolism , Serine-Arginine Splicing Factors
17.
Nucleic Acids Res ; 21(12): 2815-22, 1993 Jun 25.
Article in English | MEDLINE | ID: mdl-8332490

ABSTRACT

The U1 snRNP-specific 70K protein is one of the few snRNP proteins from higher eukaryotic cells that is phosphorylated in vivo (1,2). Immunoaffinity purified spliceosomal snRNPs (U1, U2, U5, and U4/U6) were tested for their ability to phosphorylate in vitro the U1-specific 70K protein. An snRNP-associated kinase activity which phosphorylates all U1-70K isoelectric variants was identified. Like its in vivo counterpart, this snRNP-associated enzyme phosphorylates solely serine residues of the 70K protein, preferentially utilizing ATP as a phosphodonor. Tryptic phosphopeptide analysis revealed an overlapping set of at least four radiolabeled peptides in the in vivo and in vitro phosphorylated protein, suggesting that the snRNP-associated serine kinase is responsible, at least in part, for the 70K protein phosphorylation observed in vivo. Chymotryptic digestion of in vitro, 32P-labeled 70K protein and in vitro phosphorylation studies with a synthetic peptide, indicated that the multiple 70K phosphorylation sites are limited to a highly charged, C-terminal domain of the protein. In vitro phosphorylation studies with the splicing factor ASF/SF2 and several deletion mutants demonstrated that, similar to the U1-70K protein, the snRNP-associated serine kinase phosphorylates the carboxy terminal RS-rich domain of ASF/SF2. A potential general role for this enzyme in the phosphorylation of splicing factors and its consequences for pre-mRNA splicing regulation are discussed.


Subject(s)
Arginine/metabolism , Protein Kinases/metabolism , RNA Splicing , Ribonucleoprotein, U1 Small Nuclear/metabolism , Serine/metabolism , Adenosine Triphosphate/metabolism , Amino Acid Sequence , Binding Sites , Chymotrypsin/metabolism , HeLa Cells , Humans , Molecular Sequence Data , Phosphoproteins/metabolism , Phosphorylation
18.
Cancer Res ; 52(3): 558-65, 1992 Feb 01.
Article in English | MEDLINE | ID: mdl-1732043

ABSTRACT

Biological characterization of a human colon adenocarcinoma cell line deficient in thymidylate synthase (TS-) is described. The clone, designated TS-C1/C1, was derived from the parental line GC3/C1 by selection in medium containing aminopterin, thymidine (dThd), and low concentrations of 5-formyltetrahydrofolate (5-CHO-H4PteGlu), and was subsequently reselected by single-step cloning in 500 microM methotrexate in the presence of dThd. This clone retained its TS- phenotype, was highly resistant to methotrexate (greater than 100,000-fold), and remained tumorigenic in mice (P.J. Houghton, et al., Proc. Natl. Acad. Sci. USA, 86: 1377-1381, 1989). In studies reported, it is shown that high levels of exogenous folate can support the growth of the TS- C1/C1 clone in the absence of dThd. Activation of dTMP biosynthesis de novo was demonstrated within 6 h of exposing cells to 20 microM [6R,S]5-CHO-H4PteGlu, and greater than or equal to 80% of activity was lost within 24 h of removing this folate from the medium. The labeling index was determined by autoradiographic techniques using [6-3H]2'-deoxyuridine. None of the greater than 6,000 cells radiolabeled in the absence of [6R,S]5-CHO-H4PteGlu, whereas 33.5% labeled in the presence of 20 microM exogenous folate. Relative to the parental (TS+) clone, there was a greater than 87,500-, an 8,182-, and a 425-fold higher requirement for 5-methyltetrahydrofolate ([6R,S]5-CH3-H4PteGlu), PteGlu, and [6R,S]5-CH3-H4PteGlu to support 50% maximal colony formation in the absence of dThd. Quantitative analysis of the combined pools of 5,10-methylenetetrahydrofolate (CH2-H4PteGlun) and H4PteGlun showed that parental GC3/C1 cells had higher endogenous folate pools compared to TS-C1/C1 cells [168 +/- 40 (SD) and 10.9 +/- 0.3 fmol/10(6) cells, respectively]. Qualitatively the distribution of polyglutamate species and their redistribution in cells exposed to 20 microM [6R,S]5-CHO-H4PteGlu were similar in the two lines. Analysis of pools in a second, independently derived, TS- clone (TS-C3/C3, a transcription-negative mutant) demonstrated undetectable levels of CH2-H4PteGlun and H4PteGlun. This line cannot be rescued by exogenous folate. The data thus suggest that deletion of dTMP synthase activity may cause redistribution of reduced folate pools. In cytosolic extracts from parental GC3/C1 (TS+) cells, [6R]CH2-H4PteGlu1 acted as a cofactor in the release of 3H2O from [5-3H]dUMP, whereas no activity was detected in cytosols from TS-C1/C1. In contrast dTMP synthase activity was detected in cytosols from TS- C1/C1 cells in the presence of [6R]CH2-H4PteGlu5.(ABSTRACT TRUNCATED AT 400 WORDS)


Subject(s)
Adenocarcinoma/enzymology , Colonic Neoplasms/genetics , Folic Acid/pharmacology , Leucovorin/pharmacology , Mutation , Thymidylate Synthase/genetics , Adenocarcinoma/genetics , Animals , Base Sequence , Cell Division/drug effects , Cell Line , Colonic Neoplasms/metabolism , Humans , Kinetics , Mice , Mice, Nude , Mitotic Index/drug effects , Molecular Sequence Data , Neoplasm Transplantation , Oligodeoxyribonucleotides , Phenotype , Polymerase Chain Reaction/methods , Thymidylate Synthase/metabolism , Transplantation, Heterologous , Tumor Stem Cell Assay
19.
J Biol Chem ; 264(35): 21413-21, 1989 Dec 15.
Article in English | MEDLINE | ID: mdl-2592384

ABSTRACT

This laboratory previously reported that 5-fluorouracil (FUra) increases dihydrofolate reductase (DHFR) precursor mRNA (pre-mRNA) levels relative to DHFR mRNA levels in a methotrexate-resistant KB cell line; these data suggested that incorporation of FUra into RNA may, in part, lead to cell death through the inhibition of mRNA processing (Will, C. L., and Dolnick, B.J. (1987) J. Biol. Chem. 262, 5433-5436). Utilizing a methotrexate-resistant KB cell line designated 1BT, we now report the kinetic basis for altered levels of DHFR RNA observed in FUra-treated cells. Long-term exposure to FUra had no effect on the steady-state level of DHFR pre-mRNA containing intron V or I. However, steady-state levels of total DHFR mRNA decreased 2.0-fold on a per cell basis in cells exposed to 1.0 microM FUra. No significant change in the half-life of total DHFR mRNA or pre-mRNA was observed in cells exposed to FUra (t1/2 = approximately 11.5 h and 50 min, respectively). Nuclear/cytoplasmic RNA labeling experiments demonstrated that the rate of nuclear DHFR RNA conversion to cytoplasmic DHFR mRNA decreased approximately 1.8-fold in FUra-treated cells. These results provide further evidence the FUra may inhibit processing of mRNA precursors and/or affect the stability of nuclear DHFR mRNA.


Subject(s)
Cell Nucleus/metabolism , Fluorouracil/pharmacology , Methotrexate/pharmacology , RNA Precursors/genetics , RNA Processing, Post-Transcriptional/drug effects , RNA, Messenger/genetics , Tetrahydrofolate Dehydrogenase/genetics , Blotting, Northern , Cell Nucleus/drug effects , Drug Resistance , Exons , Gene Amplification , Genes , Humans , Introns , KB Cells , Kinetics , Plasmids , RNA Precursors/drug effects , RNA, Messenger/drug effects , Restriction Mapping
20.
J Biol Chem ; 262(12): 5433-6, 1987 Apr 25.
Article in English | MEDLINE | ID: mdl-3032931

ABSTRACT

Quantitative S1 nuclease mapping studies were performed with uniformly labeled RNA probes, containing contiguous dihydrofolate reductase exon and intron sequences, and total RNA isolated from KB7B cells exposed to 5-fluorouracil for 5 days. Dihydrofolate reductase RNA containing both exon 1 and intron I, or exon 5 and a portion of intron V, increased up to 5-fold in cells grown in the presence of 2.0 to 3.0 microM 5-fluorouracil. Dihydrofolate reductase RNA containing exon 1 or exon 5, but lacking intron I or intron V, respectively, increased 2-fold in cells grown in the presence of 0.65 to 3.0 microM 5-fluorouracil. Primer extension analysis and S1 mapping studies revealed two major transcriptional start sites at positions -72 and -69 and minor start sites upstream from position -183, for dihydrofolate reductase RNA isolated from methotrexate-resistant KB7B cells. The results of these studies demonstrate that 5-fluorouracil alters the metabolism of dihydrofolate reductase precursor mRNA and/or processing intermediates.


Subject(s)
Exons/drug effects , Fluorouracil/pharmacology , Introns/drug effects , RNA, Messenger/genetics , Tetrahydrofolate Dehydrogenase/genetics , Endonucleases , Humans , KB Cells , Nucleic Acid Precursors/genetics , Nucleotide Mapping , RNA Precursors , RNA, Messenger/drug effects , Single-Strand Specific DNA and RNA Endonucleases
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