Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 188
Filter
1.
Anticancer Res ; 27(4C): 2829-32, 2007.
Article in English | MEDLINE | ID: mdl-17695455

ABSTRACT

A multifactorial grading score (MGS) for invasive squamous cell carcinoma of the uterine cervix has demonstrated its capacity to predict survival in a 5-10 year perspective and metastasis frequencies, and is a valuable tool for treatment schedules. In this study it was shown that the power of prognosis is valid even up to 20 years. In this material from 619 cervical carcinoma patients the MGS scores turned out to remain as strong as earlier proven. Earlier studies have shown that MGS is superior to other mono- and multifactorial grading systems, histological differentiation into cell types, age, clinical stage, irradiation and DNA-analysis. Treatment of cervical squamous cell carcinoma is more specific today to meet the patients' need for instance to preserve fertility or to minimize operation and eventually radiotherapy. The MGS score is a strong prognostic tool in patients with cervical carcinoma.


Subject(s)
Carcinoma, Squamous Cell/pathology , Uterine Cervical Neoplasms/pathology , Cell Differentiation/physiology , Female , Follow-Up Studies , Humans , Neoplasm Invasiveness , Neoplasm Staging , Prospective Studies , Survival Rate
2.
Colorectal Dis ; 9(6): 503-8, 2007 Jul.
Article in English | MEDLINE | ID: mdl-17573744

ABSTRACT

OBJECTIVE: Failure after ileal pouch-anal anastomosis (IPAA) is reported with a frequency of 10-20%. The failed IPAA can be excised or defunctioned. Indications for excision and further management of an indefinitely diverted pouch are poorly described. The aim of the present investigation was to investigate pouch-related problems and the histopathological pattern of the pouch mucosa in this group of patients. METHOD: In a cohort of 620 patients having IPAA with a median follow-up of 14 years, 56 patients with failure were identified. The patients with defunctioned pouches were assessed with regard to pouch-related problems and endoscopy with biopsies was performed. Biopsies were stained with haematoxylin-eosin, PAS for neutral mucins and Alcian blue/high iron diamine for sialomucins/sulphomucins. Morphological changes were grouped into three types modified according to Veress and assessed for dysplasia. RESULTS: Twenty-two patients with an indefinitely diverted pouch were found. The follow-up time after surgery for failure was 10 years. Thirteen patients completed the follow-up. Except for two patients with pelvic/perineal pain, there were no clinical problems. The majority of patients displayed mild to moderate macroscopic signs of inflammation. Morphologically, findings ranged from a preserved mucosal pattern to intense inflammatory reaction. No case of dysplasia or carcinoma was found. CONCLUSION: Most patients with an indefinitely diverted pouch had no complaints regarding the pouch. There was no case of dysplasia. Indefinite diversion may be preferable to pouch excision, especially given the associated morbidity.


Subject(s)
Colonic Pouches , Adolescent , Adult , Anastomosis, Surgical , Colonic Pouches/pathology , Female , Humans , Male , Middle Aged , Peyer's Patches/pathology , Treatment Failure
4.
Int J Gynecol Cancer ; 16(3): 1297-302, 2006.
Article in English | MEDLINE | ID: mdl-16803520

ABSTRACT

The incidence of uterine cervical cancer has increased slightly in Western countries, with an increase in relatively young women. Overexpression of matrix metalloproteinases (MMPs)-2 and -9 has turned out as a prognostic factor in many cancers. We compared the expression of the proteins MMP-2 and MMP-9 in cervical primary tumors with clinical outcome and risk factors of cervical cancer. One hundred sixty-one patients with cervical cancer treated in Umeå University Hospital or Sahlgrenska University Hospital, Sweden, between 1991 and 1995 were included in the study. Paraffin-embedded tissue samples obtained prior to treatment were examined immunohistochemically by specific antibodies for MMP-2 and MMP-9. Forty-two percent of the tumors were intensively positive for MMP-2 and 31% for MMP-9. Nineteen percent of the samples were intensively positive for both proteinases and 47% negative or weak for both. Overexpression of MMP-2 seemed to predict unfavorable survival under Kaplan-Meier analysis and in the multivariate analysis. Early sexual activity and low parity seemed to correlate to overexpression of MMP-2. MMP-9 was not associated with survival or sexual behavior. Intensive MMP-9 was noted in grade 1 tumors. We conclude that MMP-2 and MMP-9 have different roles in uterine cervical cancer. MMP-2 could be associated with aggressive behavior, but MMP-9 expression diminishes in high-grade tumors.


Subject(s)
Carcinoma/enzymology , Matrix Metalloproteinase 2/physiology , Matrix Metalloproteinase 9/physiology , Uterine Cervical Neoplasms/enzymology , Adult , Aged , Aged, 80 and over , Carcinoma/pathology , Contraceptive Agents, Female/pharmacology , Disease Progression , Female , Humans , Middle Aged , Parity , Pregnancy , Retrospective Studies , Sexual Behavior/statistics & numerical data , Survival Rate , Uterine Cervical Neoplasms/pathology
6.
Clin Exp Immunol ; 141(1): 37-46, 2005 Jul.
Article in English | MEDLINE | ID: mdl-15958068

ABSTRACT

Mice deficient for the inhibitory G protein subunit alpha2 (Galphai2(-/-)) spontaneously develop a progressive inflammatory bowel disease resembling ulcerative colitis, and have a T helper 1 (Th1)-dominated immune response prior to onset of colitis, which is further augmented after the onset of disease. The present study was performed to investigate whether the Galphai2(-/-) mice were able to down-regulate the Th1-dominated inflammatory mucosal immune response and/or induce an anti-inflammatory Th2/T regulatory response and thereby diminish the severity of colitis following treatment with acellular Bordetella pertussis vaccine. The acellular vaccine against B. pertussis, the causative agent of whooping cough, has been demonstrated to induce a Th2-mediated response in both man and mice. We therefore treated Galphai2(-/-) mice intraperitoneally with a three-component acellular B. pertussis vaccine. The treated Galphai2(-/-) mice showed significantly increased interleukin (IL)-10 production in intestinal tissue, associated with significantly reduced colitis and decreased mortality, compared to untreated Galphai2(-/-) mice. The attenuation of colitis in Galphai2(-/-) mice was due, at least partly, to the B. pertussis surface antigen filamentous haemagglutinin (FHA), which almost completely inhibited proliferation of CD4(+) T cells and stimulated apoptosis of activated CD4(+) T helper 1 cells. In conclusion, the three-component acellular B. pertussis vaccine containing filamentous haemagglutinin increases the production of IL-10 in the intestinal mucosa, induces apoptosis of activated Th1 cells and attenuates colitis in Galphai2(-/-) mice.


Subject(s)
Colitis, Ulcerative/therapy , GTP-Binding Protein alpha Subunits/deficiency , Interleukin-10/biosynthesis , Pertussis Vaccine/immunology , Th1 Cells/immunology , Adhesins, Bacterial/immunology , Animals , Antigens, Bacterial/immunology , Apoptosis/immunology , Colitis, Ulcerative/immunology , Colitis, Ulcerative/pathology , Disease Models, Animal , Hemagglutinins/immunology , Immunity, Mucosal , Immunoglobulin G/blood , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Pertussis Vaccine/therapeutic use , Survival Rate , Virulence Factors, Bordetella/immunology
7.
In Vivo ; 19(2): 383-5, 2005.
Article in English | MEDLINE | ID: mdl-15796201

ABSTRACT

A case of congenital bronchogenic cyst in the gastric mucosa is presented. The cyst was lined by pseudostratified epithelium and covered with ciliated cells. Congenital bronchogenic cysts should be differentiated from acquired gastric cysts lined with ciliated metaplastic cells that evolve as a result of environmental factors.


Subject(s)
Bronchogenic Cyst/congenital , Gastric Mucosa/pathology , Adult , Bronchogenic Cyst/pathology , Humans , Male
9.
Colorectal Dis ; 6(6): 494-8, 2004 Nov.
Article in English | MEDLINE | ID: mdl-15521942

ABSTRACT

AIM: Some of the rare complications reported in patients with an ileopouch anal anastomosis (IPAA) after coloectomy for chronic ulcerative colitis are dysplasia and carcinoma. The supposed pathway is for the ileal pouch mucosa to go through adaptational changes then is to progress through the phases of chronic pouchitis, dysplasia and subsequently to adenocarcinoma. In many of these studies however, the dysplasia-cancer sequence is inconclusive since the carcinoma might have developed from the ileal mucosa itself or from residual viable rectal mucosa left behind. The purpose of this study was therefore to study the long-term ileal mucosal adaptation patterns and the incidence and grading of dysplasia in the ileal pouch mucosa in patients previously operated on for ulcerative proctocolitis. PATIENTS AND METHODS: Forty-five patients who had been operated on with an IPAA (25 males/20 females), with a median age of 54 years (range 34-76), were invited for clinical examination and pouch endoscopy including mucosal biopsies. The duration of their colitis until surgery was median 6 years (range 1-28) and the time median interval from start of disease until time of follow up 24.8 years (range 17-46). Three independent pathologists from two different centres reviewed sequential mucosal biopsies taken from separate sites of the pouch for dysplasia and mucosal adaptation patterns. RESULTS: The type C pattern with a severe inflammation in lamina propria together with severe atrophy of villi, sometimes with ulceration and granulation tissue, was observed by the two pathologists from one centre in 15 of 45 (33.3%) patients and in 11 (24.4%) of 45 by the third pathologist, respectively. As regards dysplasia one pathologist group evaluated 2/45 (4.4%) cases as low-grade dysplasia while the third pathologist considered one of these cases as indefinite for dysplasia and one as reactive. There was in this respect full agreement between the two centres in 43 (95.6%) of 45 cases. Neither high-grade dysplasia nor invasive carcinoma was diagnosed. CONCLUSION: Dysplastic transformation within the ileal pouch mucosa in patients operated for ulcerative proctocolitis is rare even after a long follow-up. These results are reassuring for both patients and surgeons. There seem to be no solid grounds to support routine surveillance for dysplasia in the ileal pouch mucosa in these patients. The surveillance for neoplastic changes in the remaining muscular/epithelial cuff is a separate issue however.


Subject(s)
Cell Transformation, Neoplastic/pathology , Colitis, Ulcerative/surgery , Colonic Pouches/pathology , Intestinal Mucosa/pathology , Neoplasms/pathology , Proctocolectomy, Restorative/adverse effects , Adult , Aged , Biopsy, Needle , Cohort Studies , Colitis, Ulcerative/diagnosis , Female , Follow-Up Studies , Humans , Immunohistochemistry , Male , Middle Aged , Pouchitis/pathology , Proctocolectomy, Restorative/methods , Risk Assessment
10.
Int J Gynecol Cancer ; 12(4): 362-71, 2002.
Article in English | MEDLINE | ID: mdl-12144684

ABSTRACT

Mutations in the TP53 tumor suppressor gene have been shown to significantly correlate with poor prognosis in endometrial cancer. In the present study we have evaluated a luminometric immunoassay (LIA) for quantitative estimation of TP53 protein expression in 65 cytosol preparations from endometrial cancer, previously analyzed for mutations in TP53 exons 4-10. LIA showed high (> or = 0.6 ng/mg protein) expression of TP53 protein in all eight tumors with missense mutation, but high protein levels were also detected in 15 tumors with normal TP53 sequence. All four tumors with nonsense or frameshift mutations had low or no TP53 protein expression. LIA was further evaluated in a retrospective study of 201 cytosol samples from endometrial cancer. TP53 overexpression (> = 0.6 ng/mg protein) was observed in 22% of the tumors and correlated with nonendometrioid histology types (P = 0.005), poorly differentiated tumors (P = 0.001), higher FIGO grade (P = 0.001), DNA nondiploidy (P = 0.002), and high S-phase fraction (P = 0.03). After a median follow-up time of 6.8 years (range 0.7-9.9 years), 22 (13%) progressions were observed in the 175 patients with early stage (I-II) disease. TP53 overexpression (P = 0.04), FIGO grade 3 vs. 1 + 2 (P = 0.01), higher age (P = 0.02), and DNA nondiploidy (P < 0.001) showed significant correlation to shorter progression-free survival in these patients. We conclude that TP53 protein analysis by LIA provides an incomplete correlation to mutation status and cannot substitute for mutation analysis in assessment of prognosis in endometrial carcinoma. In comparison to TP53 overexpression and higher FIGO grades, DNA nonploidy status seems to be a better prognostic indicator to define a subset of early stage endometrial cancer patients who may benefit by adjuvant chemotherapy/radiotherapy.


Subject(s)
Endometrial Neoplasms/mortality , Gene Expression Regulation, Neoplastic , Tumor Suppressor Protein p53/genetics , Tumor Suppressor Protein p53/metabolism , Aged , Disease-Free Survival , Endometrial Neoplasms/genetics , Endometrial Neoplasms/immunology , Endometrial Neoplasms/metabolism , Female , Humans , Immunoassay , Luminescent Proteins , Mutation , Neoplasm Staging , Prognosis , Survival Analysis , Sweden
11.
Rhinology ; 40(2): 100-3, 2002 Jun.
Article in English | MEDLINE | ID: mdl-12091992

ABSTRACT

Nasal gliomas are uncommon tumours of neurogenic origin that occur sporadically. They are diagnosed with MRI and a preoperative biopsy, and surgery is the treatment of choice. Most of the gliomas emerge from the nasal cavity, but only a few cases of nasopharyngeal gliomas have been reported. We present one case of a nasopharyngeal glioma and two cases of nasal gliomas.


Subject(s)
Glioma , Nasopharyngeal Neoplasms , Nose Neoplasms , Female , Glioma/epidemiology , Glioma/pathology , Humans , Infant , Infant, Newborn , Nasal Cavity , Nasopharyngeal Neoplasms/epidemiology , Nasopharyngeal Neoplasms/pathology , Nose Neoplasms/epidemiology , Nose Neoplasms/pathology , Sweden/epidemiology
12.
Dis Colon Rectum ; 45(4): 448-52, 2002 Apr.
Article in English | MEDLINE | ID: mdl-12006923

ABSTRACT

PURPOSE: Sporadic reports of epithelial dysplasia and the occasional development of adenocarcinoma in the ileal pouch mucosa have recently appeared in the literature, pointing toward yet another long-term complication of the continent ileostomy and the pelvic pouch. The incidence of dysplasia and the risk for developing cancer has not been critically evaluated, however, and the reports are contradictory, with most having short observation times. The purpose of this study was to report long-term mucosal adaptation patterns and the incidence of dysplasia in Kock pouches after a mean follow-up of 30 years for patients previously operated on for ulcerative proctocolitis. METHODS: Two sets of two pathologists each (in Gothenburg, Sweden, and Manchester, United Kingdom) examined sequential, small-intestinal biopsy specimens from 40 patients with Kock pouch to observe long-term epithelial changes, with particular reference to the presence of dysplasia. RESULTS: There was full agreement between the two groups regarding the absence of high-grade dysplasia and invasive carcinoma (Categories 4 and 5 of the Vienna classification). There was, however, significant disagreement in reporting the frequency of low-grade and indefinite categories of dysplasia (Categories 2 and 3, of the Vienna classification). No attempt was made to report the differences within each set of pathologists. CONCLUSION: Because no case of high-grade dysplasia or invasive carcinoma was found in this study after a mean follow-up of 30 years, we conclude that it is very unlikely for invasive carcinoma to be a complication in ileal pouch mucosa.


Subject(s)
Adenocarcinoma/etiology , Adenocarcinoma/pathology , Colitis, Ulcerative/surgery , Ileal Diseases/etiology , Ileal Diseases/pathology , Ileal Neoplasms/etiology , Ileal Neoplasms/pathology , Intestinal Mucosa/pathology , Proctocolectomy, Restorative/adverse effects , Adult , Aged , Aged, 80 and over , Female , Follow-Up Studies , Humans , Ileostomy/adverse effects , Male , Middle Aged , Time Factors
13.
Colorectal Dis ; 4(6): 420-9, 2002 Nov.
Article in English | MEDLINE | ID: mdl-12790913

ABSTRACT

Formation of an ileo-anal pouch is an accepted technique following colectomy in the surgical management of ulcerative colitis (UC) and familial adenomatous polyposis (FAP). The configuration of pouches and anastomotic techniques has varied over the last two decades. The increased use of stapling devices in formation of the pouch-anal anastomosis avoids the need for endoanal mucosal stripping and may contribute to improved functional results, but leaves a 'columnar cuff' of residual rectal mucosa in situ. Concerns regarding the long-term safety of the ileo-anal pouch have been raised by reports of the occurrence of dysplasia in the pouch mucosa and 15 cases of adenocarcinoma. In UC, persistence of underlying disease in the residual rectal mucosa, anal transition zone and columnar cuff provides the site for development of dysplasia and malignancy. Pouchitis is unlikely to be a major cause of dysplasia or malignancy, as long-term follow-up of patients with Koch pouches has demonstrated. In FAP, any persistent rectal mucosa and mucosa of the small intestine is at risk of adenomatous dysplasia due to the genetic alterations causing the disease. Long-term surveillance should focus on all FAP pouch patients, and in UC patients should be directed towards the diagnosis of residual rectal mucosa in the area distal to the pouch anastomosis. Specialist histopathological opinion is essential in the diagnosis of dysplasia in the ileo-anal pouch.

14.
Eur J Cancer ; 37(18): 2339-48, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11720826

ABSTRACT

Components of the urokinase plasminogen activator (u-PA) system are involved in the metastatic process, and have accordingly been associated with clinical outcome in a variety of malignant tumours. We investigated the prognostic importance of u-PA and plasminogen activator inhibitor type 1 (PAI-1) in endometrial cancer, analysed with luminometric immunoassay (LIA) and enzyme-linked immunosorbent assay (ELISA), respectively. Two different cut-off levels were used: the median and the 80th percentile-the latter because of the low progression rate for patients with early stage (I-II) endometrial cancer. After a median follow-up time of 6.8 years, univariate analysis of patients with stage I-II disease (n=188) showed that high u-PA and high PAI-1 content was associated with a shorter progression-free survival (PFS), but at different cut-off levels, uPA at the median (P=0.003), and PAI-1 at the 80th percentile (P<0.001). Among the other factors, DNA ploidy status was most strongly correlated to PFS, followed by age (continuous), International Federation of Gynaecology and Obstetrics (FIGO) grade of differentiation, S-phase fraction and progesterone receptor (PgR) status. Bivariate analyses, including ploidy and one of the factors u-PA or PAI-1, showed that both add significant prognostic information. We conclude that u-PA and PAI-1 are promising prognostic factors in early stage endometrial cancer.


Subject(s)
Endometrial Neoplasms/chemistry , Neoplasm Proteins/analysis , Plasminogen Activator Inhibitor 1/analysis , Urokinase-Type Plasminogen Activator/analysis , Adult , Aged , Aged, 80 and over , Analysis of Variance , Disease Progression , Disease-Free Survival , Enzyme-Linked Immunosorbent Assay/methods , Female , Flow Cytometry , Follow-Up Studies , Humans , Male , Middle Aged , Neoplasm Staging/methods , Ploidies , Proportional Hazards Models , Time Factors
16.
Acta Oncol ; 40(4): 491-6, 2001.
Article in English | MEDLINE | ID: mdl-11504309

ABSTRACT

The prognostic value of histopathological response to preoperative radiotherapy (50 Gy) in radically resected oral carcinomas was studied in 39 consecutive patients. Microvessel density (MVD) was evaluated for relation to radioresponse and outcome. Resected tumour tissue was examined histopathologically and response to radiotherapy was scored according to induced morphological changes. Pretreatment biopsies were stained with antibodies to von Willebrand factor to evaluate MVD in hot-spot regions, in stromal tissue and in tumour epithelial tissue. Histopathological response to radiotherapy was highly prognostic of local failures and survival (p = 0.002), though microscopic surgical radicality was obtained. In good responders to preoperative radiotherapy, the 5-year survival rate was 68% compared with 24% in poor responders. In 12 patients with local recurrence after radical surgery, 11 had poor histopathological radiotherapy responses. In univariate analysis, a high MVD score in tumour epithelium was associated with poor clinical outcome but MVD did not correlate with histopathological radiotherapy response.


Subject(s)
Carcinoma, Squamous Cell/radiotherapy , Mouth Mucosa/radiation effects , Mouth Neoplasms/radiotherapy , Neovascularization, Pathologic/pathology , Radiotherapy, High-Energy , Adult , Aged , Carcinoma, Squamous Cell/blood supply , Carcinoma, Squamous Cell/drug therapy , Carcinoma, Squamous Cell/pathology , Carcinoma, Squamous Cell/surgery , Cell Hypoxia , Chemotherapy, Adjuvant , Cobalt Radioisotopes/therapeutic use , Combined Modality Therapy , Disease-Free Survival , Dose Fractionation, Radiation , Epithelial Cells/radiation effects , Female , Humans , Life Tables , Lymphatic Metastasis , Male , Middle Aged , Mouth Neoplasms/blood supply , Mouth Neoplasms/drug therapy , Mouth Neoplasms/pathology , Mouth Neoplasms/surgery , Neoadjuvant Therapy , Neoplasm Recurrence, Local , Prognosis , Radiation Tolerance , Radioisotope Teletherapy , Radiotherapy Planning, Computer-Assisted , Radiotherapy, Adjuvant , Stromal Cells/radiation effects , Survival Analysis , Survival Rate , Treatment Outcome
17.
Scand J Gastroenterol ; 36(9): 959-62, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11521987

ABSTRACT

BACKGROUND: The etiology and pathogenesis of microscopic colitis is unknown. Whether genetic predisposition is of importance, as in many other gastrointestinal diseases, is unknown. Familial occurrence of collagenous colitis has earlier been reported only in two families. METHODS: Familial occurrence of microscopic colitis was searched for in a Swedish national microscopic colitis register. RESULTS: Familial occurrence of microscopic colitis was identified in five families. In all families a sister-sister relationship was found. Two sisters with collagenous colitis had been living apart in different Nordic countries for many years before developing the disease. In one pair, the smoking sister had collagenous colitis and the never smoking sister had lymphocytic colitis. CONCLUSIONS: Considering the relative rarity of microscopic colitis, these findings indicate that a genetic predisposition may be of importance.


Subject(s)
Colitis/genetics , Adult , Aged , Female , Genetic Predisposition to Disease , Humans , Middle Aged , Registries/statistics & numerical data , Smoking/epidemiology , Sweden
18.
J Med Microbiol ; 50(5): 430-435, 2001 May.
Article in English | MEDLINE | ID: mdl-11339250

ABSTRACT

The attachment of Helicobacter pylori to the human gastric mucosa is a complex process involving several specific structures recognised by the cell surface receptors. Sialylated multivalent high mol. wt glycoproteins have been shown to inhibit H. pylori sialic acid-specific haemagglutination. This study explored whether sialylated glycoconjugates from bovine milk could inhibit an experimental H. pylori infection in a mouse model. BALB/cA mice (6-8 weeks old) were inoculated with a mouse-passaged H. pylori strain 317p. Four weeks after infection the mice were given lactoferrin (iron-free LF or 20% iron-saturated LF) or bovine milk fat globule membrane fractions (MFGM or defatted MFGM) orally (400 mg/kg body weight) once daily for 10 days and then killed to examine for bacterial colonisation and gastritis. Mice treated with iron-free LF, 20% iron-saturated LF, MFGM or defatted MFGM showed 30%, 10%, 20% or 20% healing rates, respectively, when compared with the H. pylori-infected control. Gastric colonisation by H. pylori was remarkably decreased in all mice treated with bovine milk glycoconjugates and the inflammation score was also significantly lower in treated mice than in infected control animals. The fact that there was no significant difference between iron-free LF and iron-saturated LF or MFGM and defatted MFGM suggested that iron is not crucial for inhibition of H. pylori by lactoferrin and that the lipid part of MFGM is not important for anti-H. pylori activity. In conclusion, bovine milk glycoconjugates showed potencies to inhibit H. pylori infection in this mouse model and, therefore, could be considered as candidates for non-antibiotic strategies against H. pylori infection in man.


Subject(s)
Glycoconjugates/pharmacology , Helicobacter Infections/prevention & control , Helicobacter pylori/drug effects , Animals , Cattle , Disease Models, Animal , Gastritis/microbiology , Gastritis/pathology , Gastritis/prevention & control , Helicobacter Infections/microbiology , Lactoferrin/pharmacology , Mice , Mice, Inbred BALB C , Milk/chemistry , Stomach/drug effects , Stomach/microbiology , Stomach/pathology
19.
FEMS Immunol Med Microbiol ; 30(2): 167-72, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11267851

ABSTRACT

Eighteen Dunkin-Hartley guinea pigs and 50 NMRI mice were inoculated with Helicobacter pylori and the infection followed by culture, histopathology, antibody response, and plasma levels of the acute-phase proteins albumin, C3, and transferrin for up to 7 weeks. The immune response to H. pylori surface proteins was studied by an enzyme immunoassay (EIA) and Western immunoblot and the plasma levels of albumin, C3, and transferrin were analyzed by single radial immunodiffusion. Guinea pigs had a more severe gastritis and a higher EIA immune response than NMRI mice. Serum C3 levels were elevated in infected guinea pigs after 3 and 7 weeks indicating a systemic inflammatory response and a possible link between H. pylori infection and extragastric manifestations such as vasculitis associated with atherosclerosis. Serum cholesterol levels were analyzed in guinea pigs at 7 weeks and indicated a higher level in H. pylori-infected than in control animals, but this difference was not statistically significant.


Subject(s)
Complement C3/metabolism , Helicobacter Infections/metabolism , Helicobacter pylori/pathogenicity , Acute-Phase Reaction , Animals , Cholesterol/blood , Disease Models, Animal , Guinea Pigs , Helicobacter Infections/blood , Helicobacter Infections/immunology , Male , Mice
20.
Comp Med ; 51(5): 418-23, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11924801

ABSTRACT

PURPOSE: Helicobacter pylori is a human gastroduodenal pathogen associated with type-B gastritis and gastric cancer. Low gastric tissue antioxidant levels are believed to increase the risk of developing gastric cancer. We investigated whether dietary antioxidant levels protect against infection and type-B gastritis in H. pylori-infected guinea pigs. METHODS: Dunkin-Hartley guinea pigs infected for 6 weeks with H. pylori were fed diets with various antioxidant levels. Stomach specimens were cultured, and gastritis was graded from 0 to 3. RESULTS: Supplementation with vitamins A, C, and E and with selenium yielded H. pylori recovery from 17% of challenged animals, compared with 43% of those fed a control diet. Gastritis was scored at 0.33 and 0.93, respectively. Supplementation with only vitamin C or astaxanthin had less effect on gastritis and recovery rate. In a second experiment, gastritis score in a group given vitamins A, C, E, and selenium and beta-carotene was 2.25 and in a control group, it was 2.57. The H. pylori recovery rate was 75 and 100%, respectively, with fewer colonies from animals given antioxidant supplementation (P < 0.05). CONCLUSIONS: A combination of antioxidants can protect against H. pylori infection in guinea pigs. In animal studies, antioxidant intake should be low to optimize development of H. pylori-associated disease. Furthermore we established that H. pylori causes severe gastritis in guinea pigs.


Subject(s)
Helicobacter Infections/prevention & control , Helicobacter pylori , Selenium/administration & dosage , Vitamins/administration & dosage , beta Carotene/administration & dosage , Animals , Antioxidants/administration & dosage , Ascorbic Acid/administration & dosage , Diet , Gastritis/etiology , Gastritis/microbiology , Gastritis/pathology , Gastritis/prevention & control , Guinea Pigs , Helicobacter Infections/etiology , Helicobacter Infections/microbiology , Helicobacter Infections/pathology , Helicobacter pylori/growth & development , Helicobacter pylori/isolation & purification , Helicobacter pylori/pathogenicity , Humans , Male , Vitamin A/administration & dosage , Vitamin E/administration & dosage
SELECTION OF CITATIONS
SEARCH DETAIL
...