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1.
Methods Mol Biol ; 789: 271-85, 2011.
Article in English | MEDLINE | ID: mdl-21922414

ABSTRACT

Antibody-coated microprobes have been demonstrated to be useful for detecting the release of neuropeptide transmitters from discrete sites in the central nervous system (CNS). This technique uses glass micropipettes taken through a series of chemical coatings, starting with a γ-aminopropyltriethoxysilane solution and ending with the antibody specific to the peptide transmitter of interest. The key to the reliability and repeatability of the technique is a uniform, even coating of the siloxane polymer to the glass micropipette. The microprobes, as they are called following the completion of the coating process, are inserted stereotaxically into a specific area of the CNS and the physiological intervention is performed. Tip diameters are around 5-10 µm and, depending on the length of the pipette inserted into the CNS, diameters of the pipette shaft will approach 40-50 µm. Once removed, the microprobe is then incubated with the radiolabeled peptide. Binding of the radiolabeled peptide will occur to the antibody sites not occupied by the endogenously released peptide. The images of the microprobes on sensitive autoradiographic film are analyzed for differences in the optical density along a specified length of probe. Areas of lighter density signify sites along the microprobe where endogenous peptide was biologically released during the physiological intervention. Knowing the exact location of the probe tip in vivo in the CNS permits identification of neurophysiological sites corresponding along the length of the microprobe where the peptide was released.


Subject(s)
Antibodies/metabolism , Neuropeptides/metabolism , Central Nervous System/metabolism
2.
Am J Physiol Regul Integr Comp Physiol ; 295(5): R1519-28, 2008 Nov.
Article in English | MEDLINE | ID: mdl-18753268

ABSTRACT

During myocardial ischemia, the cranial cervical spinal cord (C1-C2) modulates the central processing of the cardiac nociceptive signal. This study was done to determine 1) whether C2 SCS-induced release of an analgesic neuropeptide in the dorsal horn of the thoracic (T4) spinal cord; 2) if one of the sources of this analgesic peptide was cervical propriospinal neurons, and 3) if chemical inactivation of C2 neurons altered local T4 substance P (SP) release during concurrent C2 SCS and cardiac ischemia. Ischemia was induced by intermittent occlusion of the left anterior descending coronary artery (CoAO) in urethane-anesthetized Sprague-Dawley rats. Release of dynorphin A (1-13), (DYN) and SP was determined using antibody-coated microprobes inserted into T4. SCS alone induced DYN release from laminae I-V in T4, and this release was maintained during CoAO. C2 injection of the excitotoxin, ibotenic acid, prior to SCS, inhibited T4 DYN release during SCS and ischemia; it also reversed the inhibition of SP release from T4 dorsal laminae during C2 SCS and CoAO. Injection of the kappa-opioid antagonist, nor-binaltorphimine, into T4 also allowed an increased SP release during SCS and CoAO. CoAO increased the number of Fos-positive neurons in T4 dorsal horns but not in the intermediolateral columns (IML), while SCS (either alone or during CoAO) minimized this dorsal horn response to CoAO alone, while inducing T4 IML neuronal recruitment. These results suggest that activation of cervical propriospinal pathways induces DYN release in the thoracic spinal cord, thereby modulating nociceptive signals from the ischemic heart.


Subject(s)
Dynorphins/metabolism , Myocardial Ischemia/physiopathology , Neurons/physiology , Spinal Cord/metabolism , Spinal Cord/physiology , Animals , Brain Stem/cytology , Brain Stem/drug effects , Brain Stem/metabolism , Coronary Vessels/physiology , Electric Stimulation , Excitatory Amino Acid Agonists/pharmacology , Ibotenic Acid/pharmacology , Image Processing, Computer-Assisted , Immunohistochemistry , Male , Myocardial Ischemia/metabolism , Naltrexone/administration & dosage , Naltrexone/analogs & derivatives , Naltrexone/pharmacology , Narcotic Antagonists/administration & dosage , Narcotic Antagonists/pharmacology , Neural Pathways/drug effects , Neural Pathways/physiology , Neurons/drug effects , Neurons/metabolism , Posterior Horn Cells/drug effects , Posterior Horn Cells/metabolism , Proto-Oncogene Proteins c-fos/metabolism , Rats , Rats, Sprague-Dawley , Spinal Cord/physiopathology , Substance P/metabolism
3.
Am J Physiol Regul Integr Comp Physiol ; 294(1): R93-101, 2008 Jan.
Article in English | MEDLINE | ID: mdl-17989135

ABSTRACT

The upper cervical spinal region functions as an intraspinal controller of thoracic spinal reflexes and contributes to neuronal regulation of the ischemic myocardium. Our objective was to determine whether stimulation of the C2 cervical spinal cord (SCS) of rats modified the input signal at the thoracic spinal cord when cardiac ischemia-sensitive (sympathetic) afferents were activated by transient occlusion of the left anterior descending coronary artery (CoAO). Changes in c-Fos expression were used as an index of neuronal activation within the spinal cord and brain stem. The pattern of substance P (SP) release, a putative nociceptive transmitter, was measured using antibody-coated microprobes. Two SCS protocols were used: reactive SCS, applied concurrently with intermittent CoAO and preemptive, sustained SCS starting 15 min before and continuing during the repeated intermittent CoAO. CoAO increased SP release from laminae I and II in the T4 spinal cord above resting levels. Intermittent SCS with CoAO resulted in greater levels of SP release from deeper laminae IV-VII in T4 than CoAO alone. In contrast, SP release from laminae I and II was inhibited when CoAO was applied during preemptive, sustained SCS. Preemptive SCS likewise reduced c-Fos expression in the T4 spinal cord (laminae I-V) and nucleus tractus solitarius but increased expression in the intermediolateral cell column of T4 compared with CoAO alone. These results suggest that preemptive SCS from the high cervical region modulates sensory afferent signaling from the ischemic myocardium.


Subject(s)
Cervical Vertebrae , Myocardial Ischemia/metabolism , Neurons, Afferent/physiology , Signal Transduction/physiology , Spinal Cord/metabolism , Substance P/metabolism , Animals , Brain Stem/metabolism , Coronary Vessels/physiopathology , Electric Stimulation , Male , Proto-Oncogene Proteins c-fos/metabolism , Rats , Rats, Sprague-Dawley , Solitary Nucleus/physiology , Thoracic Vertebrae
4.
Am J Physiol Regul Integr Comp Physiol ; 287(6): R1468-77, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15297264

ABSTRACT

Electrostimulatory forms of therapy can reduce angina that arises from activation of cardiac nociceptive afferent fibers during transient ischemia. This study sought to determine the effects of electrical stimulation of left thoracic vagal afferents (C(8)-T(1) level) on the release of putative nociceptive [substance P (SP)] and analgesic [dynorphin (Dyn)] peptides in the dorsal horn at the T(4) spinal level during coronary artery occlusion in urethane-anesthetized Sprague-Dawley rats. Release of Dyn and SP was measured by using antibody-coated microprobes. While Dyn and SP had a basal release, occlusion of the left anterior descending coronary artery only affected SP release, causing an increase from lamina I-VII. Left vagal stimulation increased Dyn release, inhibited basal SP release, and blunted the coronary artery occlusion-induced release of SP. Dyn release reflected activation of descending pathways in the thoracic spinal cord, because vagal afferent stimulation still increased the release of Dyn after bilateral dorsal rhizotomy of T(2)-T(5). These results indicate that electrostimulatory therapy, using vagal afferent excitation, may induce analgesia, in part, via inhibition of the release of SP in the spinal cord, possibly through a Dyn-mediated neuronal interaction.


Subject(s)
Dynorphins/metabolism , Myocardial Ischemia/physiopathology , Spinal Cord/metabolism , Substance P/metabolism , Vagus Nerve/physiology , Afferent Pathways/physiology , Animals , Blood Pressure , Female , Heart Rate , Male , Nerve Fibers/physiology , Rats , Rats, Sprague-Dawley , Spinal Cord/physiopathology , Thoracic Vertebrae , Vagus Nerve/physiopathology
5.
Am J Physiol Heart Circ Physiol ; 287(6): H2728-38, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15284072

ABSTRACT

The purpose of this study was to identify central neuronal sites activated by stimulation of cardiac ischemia-sensitive afferent neurons and determine whether electrical stimulation of left vagal afferent fibers modified the pattern of neuronal activation. Fos-like immunoreactivity (Fos-LI) was used as an index of neuronal activation in selected levels of cervical and thoracic spinal cord and brain stem. Adult Sprague-Dawley rats were anesthetized with urethane and underwent intrapericardial infusion of an "inflammatory exudate solution" (IES) containing algogenic substances that are released during ischemia (10 mM adenosine, bradykinin, prostaglandin E2, and 5-hydroxytryptamine) or occlusion of the left anterior descending coronary artery (CoAO) to activate cardiac ischemia-sensitive (nociceptive) afferent fibers. IES and CoAO increased Fos-LI above resting levels in dorsal horns in laminae I-V at C2 and T4 and in the caudal nucleus tractus solitarius. Dorsal rhizotomy virtually eliminated Fos-LI in the spinal cord as well as the brain stem. Neuromodulation of the ischemic signal by electrical stimulation of the central end of the left thoracic vagus excited neurons at the cervical and brain stem level but inhibited neurons at the thoracic spinal cord during IES or CoAO. These results suggest that stimulation of the left thoracic vagus excites descending inhibitory pathways. Inhibition at the thoracic spinal level that suppresses the ischemic (nociceptive) input signal may occur by a short-loop descending pathway via signals from cervical propriospinal circuits and/or a longer-loop descending pathway via signals from the nucleus tractus solitarius.


Subject(s)
Heart/innervation , Myocardial Ischemia/physiopathology , Proto-Oncogene Proteins c-fos/metabolism , Solitary Nucleus/physiology , Spinal Cord/physiology , Animals , Cervical Vertebrae , Electric Stimulation , Female , Male , Neurons, Afferent/physiology , Pain/physiopathology , Rats , Rats, Sprague-Dawley , Solitary Nucleus/cytology , Spinal Cord/cytology , Thoracic Vertebrae , Vagus Nerve/cytology , Vagus Nerve/physiology
6.
Am J Physiol Heart Circ Physiol ; 286(5): H1654-64, 2004 May.
Article in English | MEDLINE | ID: mdl-14684370

ABSTRACT

Antibody-coated microprobes were inserted into the thoracic (T3-4) spinal cord in urethane-anesthetized Sprague-Dawley rats to detect the differences in the release of immunoreactive substance P-like (irSP) substances in response to differential activation of cardiac nociceptive sensory neurons (CNAN). CNAN were stimulated either by intrapericardial infusion of an inflammatory ischemic exudate solution (IES) containing algogenic substances (i.e., 10 mM each of adenosine, bradykinin, prostaglandin E2, and 5-hydroxytryptamine), or by transient occlusion of the left anterior descending coronary artery (CoAO). There was widespread basal release of irSP from the thoracic spinal cord. Stimulation of the CNAN by IES did not alter the pattern of release of irSP. Conversely, CoAO augmented the release of irSP from T3-4 spinal segments from laminae I-VII. This CoAO-induced irSP release was eliminated after thoracic dorsal rhizotomy. These results indicate that heterogeneous activation of cardiac afferents, as with focal coronary artery occlusion, represents an optimum input for activation of the cardiac neuronal hierarchy and for the resultant perception of angina. Excessive stimulation of cardiac nociceptive afferent neurons elicited during regional coronary artery occlusion involves the release of SP in the thoracic spinal cord and suggests that local spinal cord release of SP may be involved in the neural signaling of angina.


Subject(s)
Heart/innervation , Myocardial Ischemia/metabolism , Neurons, Afferent/metabolism , Spinal Cord/metabolism , Substance P/metabolism , Animals , Coronary Disease/physiopathology , Exudates and Transudates/metabolism , Female , Male , Myocardial Ischemia/physiopathology , Nociceptors/physiopathology , Rats , Rats, Sprague-Dawley , Thoracic Vertebrae
7.
Brain Res ; 963(1-2): 26-42, 2003 Feb 14.
Article in English | MEDLINE | ID: mdl-12560109

ABSTRACT

Substance P (SP) is released from the feline nucleus tractus solitarius (NTS) in response to activation of skeletal muscle afferent input. However, there are differing results about SP release from the rostral NTS in response to baroreceptor afferent input. An anti-sense oligonucleotide to feline SP (SP-asODN) was injected directly into the rostral NTS of chloralose-anesthetized cats to determine whether blood pressure or heart rate responses to ergoreceptor activation (muscle contraction) or baroreceptor unloading (carotid artery occlusion) were sensitive to SP knockdown. Control injections included either buffer alone or a scrambled-sequenced oligonucleotide (SP-sODN). Both muscle contractions and carotid occlusions were performed 3, 6 and 12 h after the completion of the oligonucleotide injections. The cardiovascular responses to contractions were significantly attenuated 3 and 6 h after SP-asODN, but not by the injection of the SP-sODN. The cardiovascular responses to contractions returned to control levels 12 h post anti-sense injection. No detectable release of SP (using antibody-coated microprobes) was measured 3 and 6 h after SP-asODN injections and the expression of SP-immunoreactivity (SP-IR) in the NTS was significantly attenuated, as determined by immunohistochemistry procedures. In contrast, neither the injection of SP-asODN nor the s-ODN attenuated the cardiovascular responses to carotid occlusions, or altered the pattern of release of SP from the brainstem. Injection of the SP-sODN did not affect the expression of SP-IR. These results suggest that the SP involved with mediating the peripheral somatomotor signal input to the rostral NTS comes from SP-containing neurons within the NTS. Our results also suggest that SP in the rostral NTS does not play a direct role in mediating the cardiovascular responses to unloading the carotid baroreceptors. We suggest that the SP released during isometric contractions excites an inhibitory pathway modulating baroreceptor input, thus contributing to the increase in mean blood pressure.


Subject(s)
Hemodynamics/physiology , Mechanoreceptors/drug effects , Oligonucleotides, Antisense/pharmacology , Pressoreceptors/drug effects , Solitary Nucleus/physiology , Substance P/physiology , Afferent Pathways/drug effects , Animals , Baroreflex/drug effects , Baroreflex/physiology , Base Sequence , Blood Pressure/drug effects , Cats , Heart Rate/drug effects , Hemodynamics/drug effects , Image Processing, Computer-Assisted , Immunohistochemistry , Microinjections , Molecular Sequence Data , Protein Precursors/biosynthesis , Protein Precursors/metabolism , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Signal Transduction/physiology , Solitary Nucleus/drug effects , Stereotaxic Techniques , Substance P/biosynthesis , Tachykinins/antagonists & inhibitors , Tachykinins/biosynthesis , Tachykinins/metabolism
8.
Neurosci Lett ; 334(1): 53-7, 2002 Dec 06.
Article in English | MEDLINE | ID: mdl-12431774

ABSTRACT

Antibody coated microprobes, inserted into the spinal cord at the L4-5 level, were used to detect whether endomorphin-2 (Endo2) was released from spinal dorsal horns in anesthetized rats in response to formalin injected into the hindpaw footpads. Saline injections were used as a control and substance P (SP) was measured to verify activation of nociceptive afferent fibers. SP but not Endo2 was released during pre-stimulation periods. Saline injections did not cause the release of either Endo2 or SP from the spinal cord. Formalin injections caused an increase in Fos expression as well as a release of SP, but not Endo2 from the ipsilateral side dorsal horn in L4-5. We conclude that Endo2 does not play a role in mediating the in vivo responses to acute inflammatory nociceptive signals at the spinal level in the anesthetized rat model.


Subject(s)
Oligopeptides/metabolism , Pain/physiopathology , Posterior Horn Cells/physiology , Animals , Female , Formaldehyde/pharmacology , Genes, fos/physiology , Hindlimb/innervation , Male , Posterior Horn Cells/drug effects , Rats , Rats, Sprague-Dawley , Substance P/metabolism
9.
Brain Res ; 944(1-2): 19-31, 2002 Jul 19.
Article in English | MEDLINE | ID: mdl-12106662

ABSTRACT

Substance P (SP) is associated with metabo- and mechanoreceptor afferent fibers ('ergoreceptors') in skeletal muscle as well as the afferent fibers from carotid sinus baroreceptors. Afferent activity from each of these are at least partially integrated in the nucleus tractus solitarius (NTS). The purpose of this study was to determine whether SP was released from the NTS during acute reflex-induced changes in blood pressure caused by stimulating these receptors. Both the muscle pressor response and the baroreflex were studied in adult cats anaesthetized with alpha-chloralose. SP antibody-coated microprobes were used to measure the possible release of SP from the NTS. The muscle pressor response caused a release of immunoreactive SP-like substances (irSP) from the rostral medial NTS, as well as the dorsal motor nucleus (DMV) and lateral tegmental field (FTL). This release was not dependent on intact afferent input from the carotid sinus nerve, but was a function of activation of muscle ergoreceptors, since no irSP was released in response to stimulation of the motor nerves after the muscle was paralyzed. There was no detectable release of irSP from the mNTS during carotid artery occlusions (baroreceptor unloading). Baroreceptor activation, induced by the i.v. injection of the vasoconstrictor, phenylephrine, did not cause the release of irSP from the mNTS above resting baseline levels. These data suggest that SP is involved with the mediation of the afferent signal from muscle ergoreceptor fibers in the medial NTS. SP is not involved with the mediation of baroreceptor afferent signaling in the medial NTS. The release of SP in response to ergoreceptors activation may function to excite an inhibitory pathway which inhibits baroreflex signals that would tend to reduce the blood pressure and heart rate during the muscle pressor response.


Subject(s)
Afferent Pathways/metabolism , Mechanoreceptors/metabolism , Muscle, Skeletal/innervation , Neurons/metabolism , Physalaemin/analogs & derivatives , Pressoreceptors/metabolism , Solitary Nucleus/metabolism , Substance P/metabolism , Afferent Pathways/drug effects , Animals , Baroreflex/drug effects , Baroreflex/physiology , Cardiovascular Physiological Phenomena/drug effects , Cats , Mechanoreceptors/drug effects , Movement/drug effects , Movement/physiology , Muscle Contraction/drug effects , Muscle Contraction/physiology , Muscle Fatigue/drug effects , Muscle Fatigue/physiology , Muscle, Skeletal/physiology , Neural Inhibition/drug effects , Neural Inhibition/physiology , Neurokinin-1 Receptor Antagonists , Neurons/cytology , Neurons/drug effects , Physalaemin/pharmacology , Pressoreceptors/drug effects , Receptors, Neurokinin-1/metabolism , Solitary Nucleus/cytology , Solitary Nucleus/drug effects , Sympathetic Nervous System/cytology , Sympathetic Nervous System/drug effects , Sympathetic Nervous System/metabolism
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