Sensory quality of meat from lambs fed garlic.

The objective of this study was to determine whether the inclusion of garlic in a ration would have a negative impact on the flavour of lamb. The study used meat from 31 Merino wether lambs fed diets with varying levels of garlic (0%, 1.8% and 3.6%) for 10 weeks. Cooked samples of meat from the lambs were assessed for flavour and acceptability as lamb by an untrained consumer panel. There was no difference (P>0.05) between the treatments in flavour score, but the 3.6% garlic treatment group scored significantly higher in terms of acceptability as lamb (P<0.05) and was commented on positively by the panellists more frequently than the meat from any other treatment (P<0.05). These results suggest that the inclusion of garlic into the animals' feed did not have a negative impact on the flavour of the lamb and, at the high rate (3.6%), made the meat more acceptable to the panellists.

Gait and postural stability in obese and nonobese prepubertal boys.

OBJECTIVE: To examine differences in gait and postural stability of obese and nonobese prepubertal boys. DESIGN: Percentage of gait cycle in double support was examined to determine significant differences. Postural stability was compared using temporal and frequency measures of the center of pressure in static stance. SETTING: Gait was examined using videography on a 30-meter walkway. Postural stability was examined using a measurement platform. PARTICIPANTS: Ten obese (>95th percentile in body mass index) and 10 nonobese (15th to 90th percentile in body mass index) prepubertal boys aged 8 to 10yrs. INTERVENTION: Participants were examined at three walking cadences as determined by preferred gait cadence. Full vision, darkness, and visually confused conditions were used to accentuate static postural stability differences. MAIN OUTCOME MEASURES: In the presence of dynamic stability differences (gait), static stability measures further investigated stability differences. RESULTS: Obese boys spent significantly (p < .02) greater percentage of gait cycle in dual stance. Obese boys showed significantly (p < .01) greater sway areas, energy, and variability primarily in the medial/lateral direction. CONCLUSIONS: Dual stance differences suggest diminished dynamic stability in obese boys. Greater sway areas in medial/lateral direction in obese boys and the absence of significant frequency measures suggest that the instability observed in obese boys is caused by excess weight rather than underlying postural instability.

Development of bilateral motor control in children with developmental coordination disorders.

This research examined behavioral (i.e. movement time) and neuromuscular (EMG) characteristics of unilateral and bilateral aiming movements of children with normal motor development and children with developmental coordination disorders (DCD). Two age groups of children were studied: 6 to 7, and 9 to 10 year olds. Bilateral aiming movements involved moving the two hands to targets of either (1) the same amplitude--symmetrical bilateral movements, or (2) different amplitudes--asymmetrical bilateral movements. Unilateral aiming movements involved moving one hand to either near or far targets associated with that hand. In general, unilateral and bilateral movement times were slower in younger than older children, and in children with DCD than children with normal motor development. Our neuromuscular data suggest that the faster movement times that typically accompany increasing age in children may be the result of a change in the capacity to initiate antagonist muscle contractions. The prolonged burst of agonist activity and delayed onset of antagonist activity observed in children with DCD may contribute to their inability to produce fast, accurate unilateral movements. On both symmetrical and asymmetrical bilateral aiming movements, children with DCD had more performance errors and greater temporal inconsistencies between neuromuscular (EMG) parameters and behavioral (movement time) parameters than children with normal motor development. These new neuromuscular data suggest that there are important differences in the way the motor control systems of children with and without DCD organize bilateral aiming responses.

Methanol promotes atmospheric methane oxidation by methanotrophic cultures and soils.

Two methanotrophic bacteria, Methylobacter albus BG8 and Methylosinus trichosporium OB3b, oxidized atmospheric methane during batch growth on methanol. Methane consumption was rapidly and substantially diminished (95% over 9 days) when washed cell suspensions were incubated without methanol in the presence of atmospheric methane (1.7 ppm). Methanotrophic activity was stimulated after methanol (10 mM) but not methane (1,000 ppm) addition. M. albus BG8 grown in continuous culture for 80 days with methanol retained the ability to oxidize atmospheric methane and oxidized methane in a chemostat air supply. Methane oxidation during growth on methanol was not affected by methane deprivation. Differences in the kinetics of methane uptake (apparent K(m) and V(max)) were observed between batch- and chemostat-grown cultures. The V(max) and apparent K(m) values (means +/- standard errors) for methanol-limited chemostat cultures were 133 +/- 46 nmol of methane 10 cells h and 916 +/- 235 ppm of methane (1.2 muM), respectively. These values were significantly lower than those determined with batch-grown cultures (V(max) of 648 +/- 195 nmol of methane 10 cells h and apparent K(m) of 5,025 +/- 1,234 ppm of methane [6.3 muM]). Methane consumption by soils was stimulated by the addition of methanol. These results suggest that methanol or other nonmethane substrates may promote atmospheric methane oxidation in situ.

Alteration in plasmid DNA following natural transformation to populations of marine bacteria.

This article examines alterations in a broad-host-range plasmid (pQSR50) that were observed following transfer to indigenous marine bacteria by natural transformation. Plasmid DNA from the transformants had altered restriction profiles. However, with the exception of the EcoRI site from one transformant (BS10), fragments amplified by polymerase chain reaction (PCR) and encompassing the recognition sites were cleaved by the relevant endonucleases, providing the sites were present. Analysis with DpnI and MboI indicated differences in DNA methylation between pQSR50 and the transformants. The missing EcoRI site from BS10 and smaller EcoRI fragments observed in transformants indicated that rearrangements had also occurred. Evolution of novel plasmid molecules following gene transfer may be an important mechanism by which natural genetic diversity is generated.

Spectral characteristics of postural control in elderly individuals.

OBJECTIVE: To examine the feasibility of using spectral analysis techniques to identify potential biomarkers of diminished postural control in elderly individuals. DESIGN: Data from spectral signatures (derived from postural sway) of 21 young adults and 42 elderly individuals classified as "high" or "low" risk with regard to functional balance capacity were analyzed using Risk Category (3) x Sensory Condition (3) multivariate analyses of variance. Postural control was challenged by varying the visual conditions under which individuals stood on a measurement platform. RESULTS: Results indicated that measures of central tendency and dispersion of the spectral frequency distribution from medial-lateral components of sway (but not antero-posterior sway) clearly differentiated between "high" and "low" risk elderly. Low risk elderly were not different from young adults. High risk elderly exhibited greater dispersion and lower mean frequency than other groups. CONCLUSIONS: Differences in spectral characteristics of medial-lateral components of sway were more related to risk category than to age. Elderly persons with high functional balance capacity displayed characteristics similar to those of young adults. Thus, spectral frequency analysis techniques may be a clinically useful tool for identifying individuals potentially at risk of falling.

The naturally transformable marine bacterium WJT-1C formally identified as "Vibrio" is a pseudomonad.

A marine bacterial isolate, previously identified as Vibrio WJT-1C (ATCC 55351) and used as a model for investigating the process of natural transformation in the marine environment, has been further examined to determine its taxonomic identity. API 20E test strips, phenotypic testing, and flagellar staining had previously assigned the strain to the genus Vibrio, most closely related to V. campbelli. 16S rRNA analysis indicated that WJT-1C was in the Pseudomonas subgroup of the gamma proteobacteria. Bacteriophage typing and natural transformation with chromosomal DNA indicated that it was distinct from previously described marine transforming pseudomonads including Pseudomonas stutzeri strain JM300. The importance and abundance of the Pseudomonas subgroup of the gamma proteobacteria in the environment suggest that these marine strains are well suited as model organisms for describing the process and importance of natural transformation in nature.

Natural transformation in river epilithon.

Natural transformation was demonstrated in unenclosed experiments incubated in river epilithon. Strains of Acinetobacter calcoaceticus were transformed to prototrophy by either free DNA (lysates) or live donor cells. The sources of transforming DNA and recipient culture were immobilized on filters, secured to stones, and incubated midstream in the river. The transfer frequency generally increased with temperature. No transfer was detected in the river Taff below 10 degrees C. The age of the recipient culture affected the transformation frequencies in situ but did not significantly affect the transfer frequency on laboratory media. Transformation of recipient cultures which had been incorporated into the natural epilithic biofilm and transformation of the plasmid pQM17 in situ were also demonstrated. This study provides the first direct evidence of natural transformation in situ of bacteria incorporated into an indigenous community.

Age-related differences in timing control of repetitive movement: application of the Wing-Kristofferson model.

Age differences in timing control were investigated in persons between 6 and 75 years. Subjects performed a repetitive finger-tapping task, attempting to match a 550-ms interresponse interval (IRI). Response variability was analyzed using the method of Wing-Kristofferson (1973). An autocovariance technique estimated the proportion of the overall (total) IRI variability contributed by central (clock) and peripheral implementation (motor delay) processes. Significant age and gender differences were revealed only for total and clock variability. Results indicated that (a) young children (6-7 years) had higher variances than all other age groups, (b) older children (9-10 years) were more variable than young and middle-age adults, (c) variances did not differ significantly across three adult age groups (21-75 years), and (d) males were less variable than females for all age groups. In agreement with recent investigations that have used the Wing-Kristofferson methodology, this study suggests that age-related differences in timing control are largely a function of central processes.

Timing and motor control in clumsy children.

The study examined timing control in clumsy children and employed the Wing-Kristofferson (1973) model of repetitive movements in an attempt to identify the locus of timing control difficulties in clumsy children. Two groups of children classified as normal and clumsy (ages 6-7 and 9-10) performed tapping and perception of duration and loudness tasks. Results indicated that clumsy children were significantly more variable than normal children in maintaining a set rate of tapping and in accurately judging time intervals. Evidence indicated that the source of timing control problems in clumsy children may be in a central timekeeping mechanism, possibly the cerebellum.

Effects of DDAVP on movement planning and execution processes in the healthy elderly.

Effects of DDAVP on speed and consistency of planning and executing simple and complex movements in healthy older adults were studied. A simple reaction time (SRT) task, a single-plane movement task, and two tasks involving multiplane movements of distal upper extremities were performed with and/or without a 0.6 ml intranasal dose (60 micrograms) of DDAVP or placebo. Results indicated that DDAVP had no significant effect on speed or consistency of SRT processes, or the speed with which simple or complex movements were planned or executed. There was also no effect on retention of motor responses.

Effects of DDAVP on movement planning and execution processes in healthy young adults.

This study investigated the effects of an acute dose of DDAVP on speed and consistency of planning and execution of simple and complex movements in healthy young adults. A simple reaction time task (SRT), a simple movement task (SMT), and a complex movement task (CMT) were performed with and without a 0.6 ml intranasal dose (60 micrograms) of DDAVP. Results indicated DDAVP-treated individuals planned and executed CMT and SRT tasks faster and more consistently than did placebo-treated subjects. There were nonsignificant DDAVP effects on speed and variability of both RT and MT processes involved in the SMT.

Lost in the wash.

I started to read Paul Elliot's cover article (Nursing Standard week ending June 3) on infection control entitled 'To wash or not to wash?' in keen expectation that he would answer his own question and tell us when, for how long and in what way we should wash our hands to minimise cross infection.

Duration of muscle activity during standing in normally and slowly developing children.

Research in motor control indicates that there are fixed patterns of activity which occur in certain muscle groups involved in standing in adults. The purpose of this study was to assess patterns of duration of muscle activity during quiet standing with and without superimposed arm movement in young children with normal and delayed motor development. Thirty-three children 4, 6, 8 and 10 years of age were screened for level of motor development and classified as either normally or slowly developing. Children performed two tasks: standing and standing with a superimposed 90 degree shoulder abduction movement. Using surface electrodes, EMG activity was monitored in Gastrocnemius, Tibialis Anterior and Erector Spinae muscle groups. The percentage of time activity was present in individual muscle groups was analyzed as a function of age and developmental level. Results indicated that quiet standing in children with delayed motor development was characterized by greater duration of activity in trunk than in leg muscles while in normal children, there was a more equal distribution of muscle activity between the legs and trunk. Both groups of children accommodated superimposed arm movement by increasing the percentage of time Erector Spinae activity was present. Patterns of duration of muscle activity in the legs were, however, different for the two groups of children.

Development of movement schema in young children.

To examine the development of movement schema in young school-age children, i.e., whether principles which govern fine eye-hand coordination skill learning as suggested by Schmidt's schema theory apply to the learning of gross motor skills Exp. 1 involved 48 right-handed first-grade children. On a modification of the Fitts Reciprocal Tapping task children moved a stylus (held in the hand or attached to a special shoe worn on the foot) between two metal targets separated by different distances. Children were randomly assigned to one of eight groups: two control or no-practice groups and six experimental or transfer groups. A one-way analysis of variance followed by appropriate Scheffé post hoc tests indicated that movements of the lower limbs were not organized into a movement schema, but a pattern of schema of movement for the upper limbs developed. That no movement schema developed for lower limb movements suggests development of movement schema is intricately linked to both the existing as well as the potential for developing precise movement in those limbs. Exp. 2 involved 40 first-grade children who were randomly assigned to perform a gross-motor agility task under one of three conditions: direct practice on the criterion task, constant practice on a modification of the criterion task, or variable practice on several different modifications of the criterion task. A groups X trials analysis of variance with appropriate post hoc tests indicated that there were no significant differences among direct, constant, or variable practice groups. Data suggest that the amount of practice may be as important as the type of practice in developing movement schema involved in gross motor skills in young children.

Descriptive analysis of static postural control in 4, 6, and 8 year old normal and motorically awkward children.

The purpose of the study was to analyze static postural control in 4, 6, and 8 year old normally and slowly developing children using qualitative electromyographic (EMG) techniques. Fourteen normally developing and six motorically awkward children performed seven different static balance tasks; twelve different muscle groups were monitored electromyographically. Results indicated that average amplitude of EMG activity involved in static postural task performance decreased with increasing age for normally developing children. Motorically awkward children displayed greater amounts of muscular activity in task performance than did normal children of similar chronological ages. Muscle activity profiles of motorically awkward children were clearly unlike those of normally developing children. The suggestion is made that one of the important characteristics of neuromuscular development in normal children is a gradual refinement of both localization and level of muscle activity.