ABSTRACT
Poor wastewater management remains a critical health and environmental challenge in most developing countries in Sub-Saharan Africa due to the lack of adequate infrastructure for collection and treatment. This study evaluated the performance and methane production of a full-scale upflow anaerobic sludge blanket (UASB) reactor of capacity 18000 m3/d, with post-treatment unit: trickling filters followed by final settling tanks for municipal wastewater treatment in Ghana. Data was collected on operational conditions and physicochemical parameters of wastewater (influent and effluent) over a period of 35 weeks in 2021 (from January to August). The influent biochemical oxygen demand to chemical oxygen demand (BOD:COD) ratio was 0.58 ± 0.16, indicating the presence of highly biodegradable compounds in the sewage. Operational conditions for the UASB reactors were observed to be within the optimal range for anaerobic systems, with an applied organic loading rate of 1.30 ± 0.79 kgCOD/m3/d. Generally, Plant performance was satisfactory with carbon removal at 93% for COD and 98% for BOD. Biogas yield was 0.2 m3/kgCOD removed, culminating in an average biogas production rate of 831.6 ± 292.7 m3/d. Average methane composition was 64.7 ± 11.9% of the biogas output, whilst an estimated 35% of the methane generated remained dissolved in the UASB effluent. The UASB reactor presents an efficient technology that can be implemented in developing countries for effective and sustainable wastewater management.
ABSTRACT
Graphene's adhesive and charge delocalization properties offer the opportunity for the direct study of biological molecule in the nanoscale regime. The inherent charge on DNA base pairs and the associated phosphate backbone can be probed by non-covalent interactions with graphene, which is a useful platform for the creation of anisotropic nanopatterned biological assemblies. Here, we report the graphene nanoribbon (GNR) supported anisotropic supramolecular self-assembly of single stranded adenine (A), cytosine (C), guanine (G), thymine (T), AT, and GC 20mer oligonucleotides, as well as the unique ordering of double stranded plasmid (circular) and Herring sperm (linear) DNA. The GNRs serve as a double sided adhesive platform for attachment to the SiO2 substrate, as well as DNA oligomers and polymers. The self-assembly is attributed to donor-acceptor interactions between DNA and graphene. These findings demonstrate that the DNA-GNR assembly yields a prospective route to novel bio-relevant nanostructures.
