Substance exposure and helmet use in all-terrain vehicle accidents: Nine years of experience at a level 1 trauma center.

INTRODUCTION: The surging popularity of all-terrain vehicles (ATV) in the United States has caused an "epidemic of injuries and mortality." The U.S. Consumer Product Safety Commission reported 99,600 injuries and 426 fatalities from ATV accidents in 2013. The aim of this study was to examine the relationship between helmet use and positive toxicology screenings on outcomes in ATV accident victims. METHODS: This is a retrospective study of patients admitted to a Level 1 Trauma Center in southwestern West Virginia following an ATV accident between 2005 and 2013. Data were obtained from the institution's Trauma Registry. RESULTS: A total of 1,857 patients were admitted during the study period with 39 (1.9%) reported deaths. Positive serum alcohol and/or urine drug screens were obtained in 66.4% of the patients tested (n=1,293). Those with positive screenings were 9.5% less likely to utilize a helmet (13.2% vs. 22.7%, p<0.001); and the lack of helmet use was associated with an increase in traumatic brain injury (57.1% vs. 41.7%, p<0.001). Positivity for substances or the lack of helmet use was significantly associated with higher morbidity. Lack of helmet use resulted in a 3.94-fold increase in the risk of discharge in a vegetative state or death. CONCLUSIONS: Drugs and alcohol use may predispose riders to be less likely to wear helmets and significantly increase the risk of a poor clinical outcome following an ATV accident. Rigorous efforts should be made to enhance safety measures through educational endeavors and amendment of current regulations to promote safe and responsible use of ATVs. PRACTICAL APPLICATIONS: Modification of regulatory requirements should be considered in order to mandate the wearing of helmets during ATV operation. In addition, expansion of safety programs should be considered in an effort to improve availability, affordability and awareness of safe ATV practices.

Streamlined expressed protein ligation using split inteins.

Chemically modified proteins are invaluable tools for studying the molecular details of biological processes, and they also hold great potential as new therapeutic agents. Several methods have been developed for the site-specific modification of proteins, one of the most widely used being expressed protein ligation (EPL) in which a recombinant α-thioester is ligated to an N-terminal Cys-containing peptide. Despite the widespread use of EPL, the generation and isolation of the required recombinant protein α-thioesters remain challenging. We describe here a new method for the preparation and purification of recombinant protein α-thioesters using engineered versions of naturally split DnaE inteins. This family of autoprocessing enzymes is closely related to the inteins currently used for protein α-thioester generation, but they feature faster kinetics and are split into two inactive polypeptides that need to associate to become active. Taking advantage of the strong affinity between the two split intein fragments, we devised a streamlined procedure for the purification and generation of protein α-thioesters from cell lysates and applied this strategy for the semisynthesis of a variety of proteins including an acetylated histone and a site-specifically modified monoclonal antibody.

Arthroscopic fixation of avulsion fractures of the tibial eminence: technique and outcome.

PURPOSE: The purpose of this study was to review an arthroscopic technique using screw or suture fixation for repair of types II and III fractures of the tibial eminence and review patient outcomes. TYPE OF STUDY: Retrospective review. METHODS: We conducted a review of 17 patients with Meyers and McKeever type II or III fractures of the tibial eminence treated with arthroscopic suture or screw fixation. We reviewed records and administered a questionnaire that included the International Knee Documentation Committee (IKDC) form, Tegner Activity scale, and Lysholm Knee Score. RESULTS: Five men and 12 women comprised the study group. Average age was 26.6 years (range, 7.5 to 60.1 years). Mean follow-up time was 32.6 months (range, 14 to 51 months). The study included 8 type II and 9 type III fractures. At follow-up evaluation, the mean Tegner score was 6.35 and mean Lysholm score was 94.2. In general, the best outcomes were seen in younger patients. For continuous age, significant differences were found for the IKDC functional scores, symptom scores, and IKDC final scores. For categorical age, younger patients had significantly better scores for the IKDC function and final scores. No significant differences were seen in outcomes with regard to fixation type. In 10 cases the intermeniscal ligament was interposed between the avulsed fracture and the tibia and was retracted or resected to allow fracture reduction. No significant differences were seen in the outcomes of these patients. CONCLUSIONS: We found that displaced tibial eminence fractures could be successfully treated in both younger and older patients using arthroscopic suture or screw fixation, with most patients returning to their previous activity levels. The interposed intermeniscal ligament must be retracted or resected to allow for anatomic fracture reduction. LEVEL OF EVIDENCE: Level III, Case Series.