1.
Anal Chem
; 70(11): 2280-7, 1998 Jun 01.
Article
in English
| MEDLINE
| ID: mdl-21644640
ABSTRACT
This paper describes the fabrication of large (up to 45 cm(2)) arrays of microwells, with volumes as small as â¼3 fL/well and densities as high as 10(7) wells/cm(2). These arrays of microwells are formed by casting an elastomer, poly(dimethylsiloxane) (PDMS), against "masters" prepared by photolithography; arrays of microwells in other polymers can be formed by using a master consisting of posts in PDMS. A straightforward technique, discontinuous dewetting, allows wells to be filled rapidly (typically on the order of 10(4) wells/s) and uniformly with a wide range of liquids. Several rudimentary strategies for addressing microwells are investigated, including electroosmotic pumping and gaseous diffusion.