Quantitative UHPSFC-MS analysis of elemental sulfur in mineral oil via derivatisation with triphenylphosphine: application to corrosive sulfur-related power transformer failure.

An ultrahigh-performance supercritical fluid chromatography-mass spectrometry (UHPSFC-MS) method has been developed as a rapid and reliable analytical method for the detection and quantification of elemental sulfur in mineral transformer oil. The method described in this paper is based on the selective reaction of elemental sulfur with triphenylphosphine (TPP). The derivatisation of elemental sulfur requires minimal sample preparation and resulted in the formation of a single compound, namely triphenylphosphine sulfide (TPPS). This derivative is quantified from the complex oil composition using electrospray ionisation-mass spectrometry (ESI-MS) in selected ion monitoring (SIM) mode, and the reported UHPSFC-MS method allows detection and quantification of the derivative at ppb levels. As sulfur contamination in mineral transformer oil has been linked to costly failures of oil/paper-based power transformers due to corrosion, the analytical approach is demonstrated through its application to mineral oil samples from in service and decommisioned power transformers. The method is ideal as a routine test or to confirm the presence of elemental sulfur in samples where corrosion has occurred.

Built environment changes and active transport to school among adolescents: BEATS Natural Experiment Study protocol.

INTRODUCTION: Natural experiments are considered a priority for examining causal associations between the built environment (BE) and physical activity (PA) because the randomised controlled trial design is rarely feasible. Few natural experiments have examined the effects of walking and cycling infrastructure on PA and active transport in adults, and none have examined the effects of such changes on PA and active transport to school among adolescents. We conducted the Built Environment and Active Transport to School (BEATS) Study in Dunedin city, New Zealand, in 2014-2017. Since 2014, on-road and off-road cycling infrastructure construction has occurred in some Dunedin neighbourhoods, including the neighbourhoods of 6 out of 12 secondary schools. Pedestrian-related infrastructure changes began in 2018. As an extension of the BEATS Study, the BEATS Natural Experiment (BEATS-NE) (2019-2022) will examine the effects of BE changes on adolescents' active transport to school in Dunedin, New Zealand. METHODS AND ANALYSIS: The BEATS-NE Study will employ contemporary ecological models for active transport that account for individual, social, environmental and policy factors. The published BEATS Study methodology (surveys, accelerometers, mapping, Geographic Information Science analysis and focus groups) and novel methods (environmental scan of school neighbourhoods and participatory mapping) will be used. A core component continues to be the community-based participatory approach with the sustained involvement of key stakeholders to generate locally relevant data, and facilitate knowledge translation into evidence-based policy and planning. ETHICS AND DISSEMINATION: The BEATS-NE Study has been approved by the University of Otago Ethics Committee (reference: 17/188). The results will be disseminated through scientific publications and symposia, and reports and presentations to stakeholders. TRIAL REGISTRATION NUMBER: ACTRN12619001335189.

Dimensions of professional competences for interventions towards sustainability.

This paper investigates sustainability competences through the eyes of professional practitioners in the field of sustainability and presents empirical data that have been created using an action research approach. The design of the study consists of two workshops, in which professional practitioners in interaction with each other and the facilitators are invited to explore and reflect on the specific knowledge, skills, attitudes and behaviours necessary to conduct change processes successfully towards sustainability in a variety of business and professional contexts. The research focuses on the competences associated with these change processes to devise, propose and conduct appropriate interventions that address sustainability issues. Labelled 'intervention competence', this ability comprises an interlocking set of knowledge, skills, attitudes and behaviours that include: appreciating the importance of (trying to) reaching decisions or interventions; being able to learn from lived experience of practice and to connect such learning to one's own scientific knowledge; being able to engage in political-strategic thinking, deliberations and actions, related to different perspectives; the ability for showing goal-oriented, adequate action; adopting and communicating ethical practices during the intervention process; being able to cope with the degree of complexity, and finally being able to translate stakeholder diversity into collectively produced interventions (actions) towards sustainability. Moreover, this competence has to be practised in contexts of competing values, non-technical interests and power relations. The article concludes with recommendations for future research and practice.

Built Environment and Active Transport to School (BEATS) Study: protocol for a cross-sectional study.

INTRODUCTION: Active transport to school (ATS) is a convenient way to increase physical activity and undertake an environmentally sustainable travel practice. The Built Environment and Active Transport to School (BEATS) Study examines ATS in adolescents in Dunedin, New Zealand, using ecological models for active transport that account for individual, social, environmental and policy factors. The study objectives are to: (1) understand the reasons behind adolescents and their parents' choice of transport mode to school; (2) examine the interaction between the transport choices, built environment, physical activity and weight status in adolescents; and (3) identify policies that promote or hinder ATS in adolescents. METHODS AND ANALYSIS: The study will use a mixed-method approach incorporating both quantitative (surveys, anthropometry, accelerometers, Geographic Information System (GIS) analysis, mapping) and qualitative methods (focus groups, interviews) to gather data from students, parents, teachers and school principals. The core data will include accelerometer-measured physical activity, anthropometry, GIS measures of the built environment and the use of maps indicating route to school (students)/work (parents) and perceived safe/unsafe areas along the route. To provide comprehensive data for understanding how to change the infrastructure to support ATS, the study will also examine complementary variables such as individual, family and social factors, including student and parental perceptions of walking and cycling to school, parental perceptions of different modes of transport to school, perceptions of the neighbourhood environment, route to school (students)/work (parents), perceptions of driving, use of information communication technology, reasons for choosing a particular school and student and parental physical activity habits, screen time and weight status. The study has achieved a 100% school recruitment rate (12 secondary schools). ETHICS AND DISSEMINATION: The study has been approved by the University of Otago Ethics Committee. The results will be actively disseminated through reports and presentations to stakeholders, symposiums and scientific publications.

Nonlinear magneto-optical rotation in the presence of a radio-frequency field.

We report measurements of nonlinear magneto-optical rotation (NMOR) for the D2 line of 87Rb atoms in an antirelaxation-coated vapor cell in the presence of a radio-frequency (rf) field. The experimental NMOR signals as a function of rf field frequency for various rf field powers are compared to a theoretical model based on the density-matrix formalism. The comparison between experiment and theory enables understanding of the ground-state atomic spin polarization dynamics, illustrated using plots of the probability distribution of the atomic angular momentum.

Spatial optical memory based on coherent population oscillations.

We show that a system characterized by long-lived coherent population oscillations (CPOs), such as a two-level system that decays via a shelving state, can be used to construct a spatial optical memory. In the presence of a cw control field, a field is generated at the four-wave mixing (FWM) frequency with the same spatial profile as the initial probe. When the control field is switched off, these spatial profiles are encoded in the CPOs of the ground and storage states. When the control field is switched on again, the probe and FWM fields are retrieved simultaneously with the same spatial profiles as the initial probe.

Efficient light storage in a Lambda system due to coupling between lower levels.

We show analytically and numerically that the application, during the storage time, of an additional weak field connecting the lower levels (LLs) of a Lambda system can modify the spin coherence and the amplitude of the retrieved probe without causing distortion. The LL coupling field adds an additional phase-dependent term to the probe propagation equation. The retrieved probe is amplified when the total relative phase Phi=0 or pi and decreased when Phi=pi/2.

Determination of in vivo disulfide-bonded proteins in Arabidopsis.

Protein thiol-disulfide oxidoreduction plays an important role in redox regulation of cellular processes. Here we present a proteomic approach to visualize and map in vivo disulfide-bonded proteins in plants. A proteomic map of the disulfide-bonded proteins was achieved using 2D gel electrophoresis of Arabidopsis protein extract. Along with novel proteins identified as potentially redox regulated, we have also shown the feasibility of mapping some of the cysteines involved in the formation of disulfide bonds. This study presents an important tool for characterizing redox-regulated proteins.

Slow and stored light in an amplifying double-Lambda system.

We show analytically and numerically that a double-Lambda system, characterized by parametric amplification of cross-coupled probe and four-wave mixing pulses, is an excellent medium for producing both slow and stored light. Moreover, this system can compensate for the absorption and broadening processes that usually occur without destroying the coherent properties of the medium. The stored probe signal pulse is written by means of a simple Lambda system and is retrieved and amplified, at the same frequency, by a double-Lambda system that also produces a pulse at the four-wave mixing frequency.

Transfer-of-coherence-enhanced stimulated emission and electromagnetically induced absorption in Zeeman split Fg-->Fe=Fg-1 atomic transitions.

The probe absorption spectra in single and multiple tripod systems formed when a weak sigma polarized pump and a tunable pi polarized probe interact with a Zeeman split F(g)-->F(e)=F(g)-1 atomic transition are characterized by two interfering stimulated Raman features separated by an electromagnetically induced absorption (EIA) peak at the line center. These Raman features can appear as either sharp stimulated emission peaks or electromagnetically induced transparency windows. In the multitripod systems, the EIA and stimulated emission peaks derive from the combined effects of interference between the stimulated Raman features and transfer of coherence from the excited to ground states.

Ultranarrow extraresonant antiholes in pump-probe spectroscopy induced by inelastic collisions.

Pump-probe spectroscopy with copropagating pump and probe beams of an open two-level system where, as a result of inelastic collisions, the lower state of an optical transition relaxes faster to reservoir states than the upper state does, may yield an antihole in the probe spectral profile whose linewidth is subnatural and unaffected by Doppler broadening.

Doppleron-induced stimulated reflection.

In a gaseous system, energy can be transferred from an intense incoming beam to a counterpropagating probe beam at the same frequency. The effect may be interpreted in terms of Doppleron processes and arises because the medium undergoes interaction with beams of different effective frequencies owing to the Doppler effect.

Polarization dependence of resonance-enhanced three-photon scattering.

The polarization properties of stimulated three-photon scattering in a three-level atomic system are presented. In our investigation of the stimulated emission spectrum of Na, we find that interference between the amplitudes of the resonant contributions of the Na doublet states results in interesting polarization phenomena. Particularly striking is the behavior when the laser is tuned near the dispersion-free point. With the laser linearly polarized the stimulated three-photon scattering is polarized perpendicular to the laser polarization. For circularly polarized excitation, total extinction of three-photon scattering is observed.