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1.
STAR Protoc ; 4(1): 102097, 2023 03 17.
Article in English | MEDLINE | ID: mdl-36853711

ABSTRACT

Dissecting mechanisms driving subclone expansion in primary cancers has been challenging. Here, we present a protocol to systematically disrupt entire gene networks and assess the functional impact of this perturbation on cancer cell fitness. By combining arrayed CRISPR libraries and high-content microscopy, we describe steps to identify classes of genes whose inactivation promotes resistance to environmental challenges faced by cancer cells during tumor growth or upon therapy. A proof-of-principle interrogation of the epigenetic regulatory network is described. For complete details on the use and execution of this protocol, please refer to Loukas et al. (2022).1.


Subject(s)
Neoplasms , Humans , Mutation/genetics , Neoplasms/genetics , Epigenomics , Gene Regulatory Networks
2.
Nat Cell Biol ; 23(4): 401-412, 2021 04.
Article in English | MEDLINE | ID: mdl-33837287

ABSTRACT

Rewiring of cellular programmes in malignant cells generates cancer-specific vulnerabilities. Here, using an unbiased screening strategy aimed at identifying non-essential genes required by tumour cells to sustain unlimited proliferative capacity, we identify the male-specific lethal (MSL) acetyltransferase complex as a vulnerability of genetically unstable cancers. We find that disruption of the MSL complex and consequent loss of the associated H4K16ac mark do not substantially alter transcriptional programmes but compromise chromosome integrity and promote chromosomal instability (CIN) that progressively exhausts the proliferative potential of cancer cells through a p53-independent mechanism. This effect is dependent on pre-existing genomic instability, and normal cells are insensitive to MSL disruption. Using cell- and patient-derived xenografts from multiple cancer types, we show that excessive CIN induced by MSL disruption inhibits tumour maintenance. Our findings suggest that targeting MSL may be a valuable means to increase CIN beyond the level tolerated by cancer cells without inducing severe adverse effects in normal tissues.


Subject(s)
Cell Proliferation/genetics , Chromosomal Instability/genetics , Multiprotein Complexes/genetics , Neoplasms/genetics , Animals , Cell Line, Tumor , Cellular Reprogramming/genetics , Chromosomal Proteins, Non-Histone/genetics , DNA-Binding Proteins/genetics , Heterografts , Histone Acetyltransferases/genetics , Humans , Mice , Neoplasms/pathology , Transcription Factors/genetics , Tumor Suppressor Protein p53/genetics , Ubiquitin-Protein Ligases/genetics
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