ABSTRACT
Drosophila melanogaster 125I-5S RNA was annealed to salivary gland preparations of 6 species in the virilis group of Drosophila. Two patterns of annealing were found. D. virilis, D. montana and D. borealis showed three 5S gene clusters on chromosome 5; Sd-f and Wc-j were strongly labeled, but Xa-e was weakly labeled. D. montana and D. borealis have a greater percentage of their total 5S cistroms at S d-f than does D. virilis. D. americana americana, D. americana texana and D. novamexicana showed 2 sites labeled; no label was seen at Sd-f while Wc-j was weakly labeled and Xa-e was strongly labeled, the reciprocal of the previous pattern in the W-X region. Hybrids between D. a americana and D. virilis showed no difference in chromosome banding at the sites of the 5S clusters despite their pattern difference. D. a texana X D. virilis, on the other hand, did show a difference in staining the Xa-e region. These patterns fall squarely into the biosystematic groupings deduced by many previous workers.
Subject(s)
Drosophila/ultrastructure , Genes , Nucleic Acid Hybridization , RNA , Animals , Chromosome Mapping , Female , Male , Ribosomes , Salivary Glands/ultrastructureSubject(s)
DNA/analysis , Genes , Nucleic Acid Hybridization , Animals , Autoradiography , Base Sequence , Cell Nucleolus/analysis , Chromosome Mapping , Chromosomes/analysis , DNA-Directed RNA Polymerases , Drosophila melanogaster , Escherichia coli/enzymology , Hemoglobins , Histones , Iodine Radioisotopes , Mutation , RNA , RNA, Bacterial , Ribosomes/analysis , Sea Urchins , Tritium , XenopusSubject(s)
Chromosomes , Nucleic Acid Hybridization , Animals , Autoradiography , Base Sequence , DNA/metabolism , Drosophila melanogaster/metabolism , Genes , Haploidy , Kinetics , Methods , Nucleic Acid Denaturation , RNA/metabolism , RNA, Ribosomal/biosynthesis , RNA, Transfer/biosynthesis , Salivary Glands/cytology , Silver , Time Factors , Transcription, Genetic , TritiumSubject(s)
Chromosomes/analysis , Drosophila melanogaster/cytology , Nucleic Acid Hybridization , RNA, Transfer/analysis , Animals , Autoradiography , Chromosome Mapping , DNA/analysis , Larva , Methods , RNA/isolation & purification , RNA, Transfer/isolation & purification , Salivary Glands/cytology , Sex Chromosomes/analysis , Statistics as Topic , Tritium , Uridine/metabolismABSTRACT
[(3)H]RNA with a high specific activity was prepared from larvae of Drosophila melanogaster grown 4 days in contact with [(3)H]uridine. Purified tritiated 5S RNA was annealed to the DNA of polytene chromosomes, which had been denatured in formamide. The 5S RNA genes are placed within the region 56E-F of the right arm of chromosome 2. This localization was determined from autoradiographs, where the radioactivity from hybrids of [(3)H]RNA and DNA was confined to the 56E-F segment.
