ABSTRACT
We present the numerical analysis of the effect of the temporarily partially coherent illumination on the phase measurement accuracy in digital holography microscopy (DHM) and optical diffraction tomography (ODT), as reconstruction algorithms tend to assume purely monochromatic conditions. In the regime of reduced temporal coherence, we simulate the hologram formation in two different optical setups, representing classical off-axis two-beam and grating common-path configurations. We consider two ODT variants: with sample rotation and angle-scanning of illumination. Besides the coherence degree of illumination, our simulation considers the influence of the sample normal dispersion, shape of the light spectrum, and optical parameters of the imaging setup. As reconstruction algorithms we employ Fourier hologram method and first-order Rytov approximation with direct inversion and nonnegativity constraints. Quantitative evaluation of the measurement results deviations introduced by the mentioned error sources is comprehensively analyzed, for the first time to the best of our knowledge. Obtained outcomes indicate low final DHM/ODT reconstruction errors for the grating-assisted common-path configuration. Nevertheless, dispersion and asymmetric spectrum introduce non-negligible overestimated refractive index values and noise, and should be thus carefully considered within experimental frameworks.
ABSTRACT
Building on Gabor seminal principle, digital in-line holographic microscopy provides efficient means for space-time investigations of large volumes of interest. Thus, it has a pivotal impact on particle tracking that is crucial in advancing various branches of science and technology, e.g., microfluidics and biophysical processes examination (cell motility, migration, interplay etc.). Well-established algorithms often rely on heavily regularized inverse problem modelling and encounter limitations in terms of tracking accuracy, hologram signal-to-noise ratio, accessible object volume, particle concentration and computational burden. This work demonstrates the DarkTrack algorithm-a new approach to versatile, fast, precise, and robust 4D holographic tracking based on deterministic computationally rendered high-contrast dark fields. Its unique capabilities are quantitatively corroborated employing a novel numerical engine for simulating Gabor holographic recording of time-variant volumes filled with predefined dynamic particles. Our solution accounts for multiple scattering and thus it is poised to secure an important gap in holographic particle tracking technology and allow for ground-truth-driven benchmarking and quantitative assessment of tracking algorithms. Proof-of-concept experimental evaluation of DarkTrack is presented via analyzing live spermatozoa. Software supporting both novel numerical holographic engine and DarkTrack algorithm is made open access, which opens new possibilities and sets the stage for democratization of robust holographic 4D particle examination.
Subject(s)
Holography , Microscopy , Algorithms , Signal-To-Noise Ratio , SoftwareABSTRACT
We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations to decide the in-focus position. The classical autofocusing algorithms apply a uniform search strategy, i.e., they probe multiple, uniformly distributed axial locations, which leads to heavy computational overhead. Our method substantially reduces the computational load, without sacrificing the accuracy, by skillfully selecting the next location to investigate, which results in a decreased total number of probed propagation distances. This is achieved by applying the golden selection search with parabolic interpolation, which is the gold standard for tackling single-variable optimization problems. The proposed approach is successfully applied to three diverse autofocusing cases, providing up to 136-fold speed-up.
ABSTRACT
In this work we propose an open-top like common-path intrinsically achromatic optical diffraction tomography system. It operates as a total-shear interferometer and employs Ronchi-type amplitude diffraction grating, positioned in between the camera and the tube lens without an additional 4f system, generating three-beam interferograms with achromatic second harmonic. Such configuration makes the proposed system low cost, compact and immune to vibrations. We present the results of the measurements of 3D-printed cell phantom using laser diode (coherent) and superluminescent diode (partially coherent) light sources. Broadband light sources can be naturally employed without the need for any cumbersome compensation because of the intrinsic achromaticity of the interferometric recording (holograms generated by -1st and +1st conjugated diffraction orders are not affected by the illumination wavelength). The results show that the decreased coherence offers much reduced coherent noise and higher fidelity tomographic reconstruction especially when applied nonnegativity constraint regularization procedure.
ABSTRACT
We develop a time-efficient computation scheme for a holographic tomography reconstruction technique that accounts for multiple scattering by applying the forward model based on the wave propagation method (WPM). The computational efficiency is achieved by employing adjoint equations for calculation of the gradient of the data fidelity term in the gradient descent optimization procedure. In the paper we provide a general computation scheme that is suitable for various forward models that can be represented in the form of an iterative equation. Next, we provide the complete solution for the time-efficient reconstruction utilizing WPM. In the considered reconstruction case, the proposed algorithm enables the 114-fold speed-up of computations with respect to the original tomographic method.
