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1.
Rhinology ; 59(4): 402-404, 2021 Aug 01.
Article in English | MEDLINE | ID: mdl-34085061

ABSTRACT

To the Editor: Monitoring of olfactory function and diagnosis of olfactory disorders using the pen-based "Sniffin' Sticks test" is problematic during the SARS-CoV-2 pandemic due to hygienic concerns. The aim of this study was to find out whether the results of olfactory testing obtained by presenting odours on a single-use filter paper is identical to performing the test by presenting the odour pens according to the manufacturer's manual.


Subject(s)
COVID-19 , Olfaction Disorders , Humans , Odorants , SARS-CoV-2 , Sensory Thresholds , Smell
2.
Rhinology ; 58(2): 184-191, 2020 Apr 01.
Article in English | MEDLINE | ID: mdl-31693018

ABSTRACT

BACKGROUND: The prevalence of olfactory impairment increases with age and is known to be an early sign of different neurodegenerative diseases. Only few population-based studies examined the prevalence of olfactory impairment and comparisons across studies are scarce. Aim of this analysis was to compare the prevalence and determinants of normosmia across five population based studies in Germany. METHODOLOGY: Data from five population-based, cross-sectional studies were included. They were independently conducted and used the same test system (Sniffin' Sticks Screening 12) to measure olfactory function. This system consists of 12 odor-dispensing felt-tip pens; the task is a forced-choice selection among four alternative odors per pen. Sociodemographic information and comorbidities were assessed in face-to-face interviews. Univariate, descriptive statistics and multivariable logistic regression models stratified by study, were performed to determine risks, i.e. prevalence odds ratios, associated with olfactory function. RESULTS: The prevalence of normosmic participants varied considerably across studies. Olfactory function was lower in men, decreased with age, and increased with higher education. Several individual comorbidities and a comorbidity index were associated with olfactory dysfunction. Recognition performance for three of the 12 pens was especially low in all studies. CONCLUSION: Four factors, well known to describe population composition, contribute to explain differences in the prevalence of olfactory function between studies when the same test system is used. Our results indicate that comorbidities and educational level should always be considered when test systems based on smell recognition are used in population-based studies.


Subject(s)
Olfaction Disorders , Smell , Cross-Sectional Studies , Germany , Humans , Male , Odorants , Prevalence , Risk Factors
3.
Br J Pharmacol ; 151(2): 226-36, 2007 May.
Article in English | MEDLINE | ID: mdl-17351651

ABSTRACT

BACKGROUND AND PURPOSE: The aim of the present study was to investigate whether the endogenous metabotropic P2Y receptors modulate ionotropic P2X(3) receptor-channels. EXPERIMENTAL APPROACH: Whole-cell patch-clamp experiments were carried out on HEK293 cells permanently transfected with human P2X(3) receptors (HEK293-hP2X(3) cells) and rat dorsal root ganglion (DRG) neurons. KEY RESULTS: In both cell types, the P2Y(1,12,13) receptor agonist, ADP-beta-S, inhibited P2X(3) currents evoked by the selective agonist, alpha,beta-methylene ATP (alpha,beta-meATP). This inhibition could be markedly counteracted by replacing in the pipette solution the usual GTP with GDP-beta-S, a procedure known to block all G protein heterotrimers. P2X(3) currents evoked by ATP, activating both P2Y and P2X receptors, caused a smaller peak amplitude and desensitized faster than those currents evoked by the selective P2X(3) receptor agonist alpha,beta-meATP. In the presence of intracellular GDP-beta-S, ATP- and alpha,beta-meATP-induced currents were identical. Recovery from P2X(3) receptor desensitization induced by repetitive ATP application was slower than the recovery from alpha,beta-meATP-induced desensitization. When G proteins were blocked by intracellular GDP-beta-S, the recovery from the ATP- and alpha,beta-meATP-induced desensitization were of comparable speed. CONCLUSIONS AND IMPLICATIONS: Our results suggest that the activation of P2Y receptors G protein-dependently facilitates the desensitization of P2X(3) receptors and suppresses the recovery from the desensitized state. Hence, the concomitant stimulation of P2X(3) and P2Y receptors of DRG neurons by ATP may result both in an algesic effect and a partly counterbalancing analgesic activity.


Subject(s)
GTP-Binding Proteins/physiology , Receptors, Purinergic P2/physiology , Adenosine Diphosphate/analogs & derivatives , Adenosine Diphosphate/pharmacology , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/pharmacology , Animals , Animals, Newborn , Cell Line , Cells, Cultured , Dose-Response Relationship, Drug , Female , Ganglia, Spinal/cytology , Ganglia, Spinal/physiology , Guanosine Diphosphate/analogs & derivatives , Guanosine Diphosphate/pharmacology , Guanosine Triphosphate/pharmacology , Humans , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Neurons/drug effects , Neurons/physiology , Patch-Clamp Techniques , Purinergic P2 Receptor Agonists , Rats , Rats, Wistar , Receptors, Purinergic P2/genetics , Receptors, Purinergic P2X3 , Thionucleotides/pharmacology , Time Factors , Transfection
4.
J Neurochem ; 79(5): 997-1003, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11739611

ABSTRACT

The present study investigated the pharmacological properties of excitatory P2X receptors and P2X(2) and P2X(5) receptor subunit expression in rat-cultured thoracolumbar sympathetic neurons. In patch-clamp recordings, ATP (3-1000 microM; applied for 1 s) induced inward currents in a concentration-dependent manner. Pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS; 30 microM) counteracted the ATP response. In contrast to ATP, alpha,beta-meATP (30 microM; for 1 s) was virtually ineffective. Prolonged application of ATP (100 microM; 10 s) induced receptor desensitization in a significant proportion of sympathetic neurons in a manner typical for P2X(2-2) splice variant-mediated responses. Using single-cell RT-PCR, P2X(2), P2X(2-2) and P2X(5) mRNA expression was detectable in individual tyrosine hydroxylase-positive neurons; coexpression of both P2X(2) isoforms was not observed. Laser scanning microscopy revealed both P2X(2) and P2X(5) immunoreactivity in virtually every TH-positive neuron. P2X(2) immunoreactivity was largely distributed over the cell body, whereas P2X(5) immunoreactivity was most distinctly located close to the nucleus. In summary, the present study demonstrates the expression of P2X(2), P2X(2-2) and P2X(5) receptor subunits in rat thoracolumbar neurons. The functional data in conjunction with a preferential membranous localization of P2X(2)/P2X(2-2) compared with P2X(5) suggest that the excitatory P2X responses are mediated by P2X(2) and P2X(2-2) receptors. Apparently there exist two types of P2X(2) receptor-bearing sympathetic neurons: one major population expressing the unspliced isoform and another minor population expressing the P2X(2-2) splice variant.


Subject(s)
Neurons/metabolism , Receptors, Purinergic P2/metabolism , Spinal Cord/metabolism , Sympathetic Nervous System/metabolism , Animals , Electrophysiology , Ganglia, Spinal/metabolism , Immunohistochemistry , Male , Microscopy, Confocal , Nerve Tissue Proteins/biosynthesis , Patch-Clamp Techniques , RNA, Messenger/biosynthesis , Rats , Rats, Wistar , Receptors, Purinergic P2/biosynthesis , Receptors, Purinergic P2X2 , Receptors, Purinergic P2X5 , Reverse Transcriptase Polymerase Chain Reaction , Spinal Cord/cytology , Sympathetic Nervous System/cytology , Tyrosine 3-Monooxygenase/metabolism
5.
Neurochem Res ; 26(8-9): 925-32, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11699944

ABSTRACT

Glutamate is the major excitatory neurotransmitter in the brain. It acts at ligand-gated cationic channels (NMDA, AMPA and kainate receptors) and at G protein-coupled metabotropic glutamate receptors as well. The glutamatergic transmission is suggested to be involved in development, learning and memory. Its dysfunction can be detected in epilepsy, stroke, neurodegenerative disorders and drug abuse. This paper summarizes the present knowledge on the modulation of glutamate-gated ion channels in the central nervous system by phosphorylation. An inhibitory interaction between adenosine A2A receptors and NMDA receptors in the neostriatum is described as an example. mediated by the phospholipase C/inositol trisphosphate/calmodulin and calmodulin kinase II pathway.


Subject(s)
Brain/metabolism , Ion Channels/physiology , Receptors, Glutamate/physiology , Animals , Ion Channels/metabolism , Phosphorylation , Receptors, Glutamate/metabolism
6.
Alcohol Clin Exp Res ; 25(5 Suppl ISBRA): 182S-188S, 2001 May.
Article in English | MEDLINE | ID: mdl-11391069

ABSTRACT

This article represents the proceedings of a symposium at the 2000 ISBRA Meeting in Yokohama, Japan. The chairs were Toshio Narahashi and Kinya Kuriyama. The presentations were (1) Modulation of neuroreceptors and ion channels by alcohol, by T. Narahashi; (2) Inhibition by ethanol of NMDA and AMPA receptor-channels, by P. Illes, K. Wirkner, W. Fischer, K. Mühlberg, P. Scheibler, and C. Allgaier; (3) Effects of ethanol on metabotropic glutamate receptors, by K. Minami; (4) Acute alcohol actions on the 5-HT3 ligand-gated ion channel, by D. Lovinger; (5) Inhibition of NMDA receptors by MK801 attenuates ethanol-induced taurine release from the hippocampus, by F. Lallemand, R.J. Ward, and P. DeWitte; and (6) Effect of ethanol on voltage-operated Ca2+ channels in hepatic stellate cells, by T. Itatsu, Y. Takei, H. Oide, M. Hirose, X. E. Wang, S. Watanabe, M. Tateyama, R. Ochi, and N. Sato.


Subject(s)
Calcium Channels/drug effects , Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Receptors, AMPA/drug effects , Receptors, Metabotropic Glutamate/drug effects , Receptors, N-Methyl-D-Aspartate/drug effects , Animals , Calcium Channels/physiology , Hippocampus/drug effects , Hippocampus/physiology , Humans , Ion Channels/drug effects , Ion Channels/physiology , Receptors, AMPA/physiology , Receptors, Metabotropic Glutamate/physiology , Receptors, N-Methyl-D-Aspartate/physiology , Receptors, Serotonin/drug effects , Receptors, Serotonin/physiology , Receptors, Serotonin, 5-HT3 , Sensory Receptor Cells/drug effects , Sensory Receptor Cells/metabolism
7.
Neuroscience ; 102(3): 593-602, 2001.
Article in English | MEDLINE | ID: mdl-11226696

ABSTRACT

Intracellular recordings were made in a mid-pontine slice preparation of the rat brain containing the nucleus locus coeruleus. Focal electrical stimulation evoked biphasic synaptic potentials consisting of early depolarizing (d.p.s.p.) and late hyperpolarizing (i.p.s.p.) components. The alpha(2)-adrenoceptor antagonist idazoxan inhibited the i.p.s.p. without altering the d.p.s.p. All of the following experiments were carried out in the presence of kynurenic acid and picrotoxin to block the glutamatergic and GABAergic fractions of the d.p.s.p., respectively. Guanethidine, which is known to inhibit noradrenaline and ATP release from nerve terminals of postganglionic sympathetic nerves, depressed both the d.p.s.p. and the i.p.s.p. in a concentration-dependent manner. Damage of catecholaminergic nerve terminals by 6-hydroxydopamine also decreased both the d.p.s.p. and the i.p.s.p. The P2 receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid (PPADS) depressed the d.p.s.p., whereas the i.p.s.p. remained unaffected. The further application of PPADS did not increase the depression of the d.p.s.p. by guanethidine. Superfusion with the mixed alpha-adrenoceptor agonist noradrenaline or the selective P2 receptor agonist adenosine 5'-O-(2-thiodiphosphate) inhibited both the d.p.s.p. and the i.p.s.p. The inhibitory effects of these agonists were prevented by the respective antagonists idazoxan or suramin. In the presence of suramin noradrenaline failed to inhibit the residual d.p.s.p. Superfused noradrenaline potentiated rather than inhibited responses to pressure-applied alpha,beta-methylene-ATP; superfused adenosine 5'-O-(2-thiodiphosphate) did not interact with pressure-applied noradrenaline. In conclusion, we present electrophysiological evidence for the co-release of ATP and catecholamines in the CNS. At the cell somata of neurons in the locus coeruleus, noradrenaline and ATP activate inhibitory alpha(2)-adrenoceptors and excitatory P2 receptors, respectively. In addition, inhibitory presynaptic autoreceptors of the alpha(2) and P2 types appear to regulate release of the two co-transmitters.


Subject(s)
Adenosine Diphosphate/analogs & derivatives , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/physiology , Catecholamines/physiology , Evoked Potentials/physiology , Excitatory Postsynaptic Potentials/physiology , Neurons/physiology , Pons/physiology , Pyridoxal Phosphate/analogs & derivatives , Synaptic Transmission/physiology , Adenosine Diphosphate/pharmacology , Adenosine Triphosphate/pharmacology , Animals , Electric Stimulation , Evoked Potentials/drug effects , Excitatory Postsynaptic Potentials/drug effects , Guanethidine/pharmacology , Idazoxan/pharmacology , In Vitro Techniques , Kynurenic Acid/pharmacology , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Neurons/drug effects , Norepinephrine/pharmacology , Oxidopamine/pharmacology , Picrotoxin/pharmacology , Pyridoxal Phosphate/pharmacology , Rats , Rats, Wistar , Synaptic Transmission/drug effects , Thionucleotides/pharmacology
8.
Naunyn Schmiedebergs Arch Pharmacol ; 363(1): 120-3, 2001 Jan.
Article in English | MEDLINE | ID: mdl-11191830

ABSTRACT

Whole-cell patch-clamp recordings were performed on 12- to 15-day-old rat locus coeruleus neurones in a midpontine slice preparation. Application of noradrenaline (100 microM) and N-methyl-D-aspartate (NMDA; 100 microM) induced a small outward current and a distinct inward current, respectively. Single-cell reverse transcriptase-polymerase chain reaction (scRT-PCR), used to analyse the expression pattern of NMDA receptor subunits 2A, 2B, and 2C (NR2A-C) subsequent to electrophysiological characterization, demonstrated differences in the capacity of individual locus coeruleus neurones to express NR2A-C mRNA. NR2C mRNA expression predominated over those of NR2A and NR2B mRNA in most neurones. In addition, in neurones containing NR2C mRNA NMDA induced significantly larger currents than in cells lacking expression of this gene. RT-PCR studies performed on tissue preparations of adult rats also revealed a distinct expression of NR2C mRNA. In conclusion, the present data demonstrate differences in the mRNA expression pattern of NR2A-C of individual locus coeruleus neurones with a predominant NR2C mRNA expression in the majority of the cells.


Subject(s)
Locus Coeruleus/metabolism , Neurons/metabolism , Receptors, N-Methyl-D-Aspartate/genetics , Animals , Excitatory Amino Acid Agonists/pharmacology , Gene Expression , Locus Coeruleus/cytology , Locus Coeruleus/physiology , Membrane Potentials/drug effects , N-Methylaspartate/pharmacology , Neurons/drug effects , Neurons/physiology , Norepinephrine/pharmacology , Patch-Clamp Techniques , Protein Subunits , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate/physiology , Reverse Transcriptase Polymerase Chain Reaction
9.
Eur J Pharmacol ; 409(3): R3-5, 2000 Dec 15.
Article in English | MEDLINE | ID: mdl-11108830

ABSTRACT

Membrane currents in response to the application of alpha, beta-methylene ATP (alpha,beta-meATP) were recorded by the whole-cell patch-clamp technique in human embryonic kidney 293 cells transfected with the human P2X3 receptor (HEK 293-hP2X3 cells). Trichloroethanol, the biologically active metabolite of chloral hydrate, but not ethanol itself concentration-dependently and reversibly inhibited the current responses. It was concluded that the reported analgesic effect of chloral hydrate may be due to the interruption of pain transmission in dorsal root ganglia expressing P2X3 receptors.


Subject(s)
Adenosine Triphosphate/analogs & derivatives , Ethylene Chlorohydrin/analogs & derivatives , Ion Channels/drug effects , Receptors, Purinergic P2/drug effects , Adenosine Triphosphate/pharmacology , Cell Line , Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Ethylene Chlorohydrin/pharmacology , Humans , Ion Channels/physiology , Membrane Potentials/drug effects , Membrane Potentials/physiology , Receptors, Purinergic P2/physiology , Receptors, Purinergic P2X3
10.
Br J Pharmacol ; 130(2): 259-69, 2000 May.
Article in English | MEDLINE | ID: mdl-10807662

ABSTRACT

Whole-cell patch clamp experiments were used to investigate the transduction mechanism of adenosine A(2A) receptors in modulating N-methyl-D-aspartate (NMDA)-induced currents in rat striatal brain slices. The A(2A) receptor agonist 2-p-(2-carboxyethyl)phenethylamino-5'-N-ethylcarboxamidoadenosine (CGS 21680) inhibited the NMDA, but not the (S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) current in a subset of striatal neurons. Lucifer yellow-filled pipettes in combination with immunostaining of A(2A) receptors were used to identify CGS 21680-sensitive cells as typical medium spiny striatal neurons. Dibutyryl cyclic AMP and the protein kinase A activator Sp-cyclic AMPs, but not the protein kinase A inhibitors Rp-cyclic AMPS or PKI(14 - 24)amide abolished the inhibitory effect of CGS 21680. The phospholipase C inhibitor U-73122, but not the inactive structural analogue U-73343 also interfered with CGS 21680. The activation of protein kinase C by phorbol 12-myristate 13-acetate or the blockade of this enzyme by staurosporine did not alter the effect of CGS 21680. Heparin, an antagonist of inositol 1, 4,5-trisphosphate (InsP(3)) and a more efficient buffering of intracellular Ca(2+) by BAPTA instead of EGTA in the pipette solution, abolished the CGS 21680-induced inhibition. The calmodulin antagonist W-7 and cytochalasin B which enhances actin depolymerization also prevented the effect of CGS 21680; the calmodulin kinase II inhibitors CaM kinase II(281 - 309) and KN-93 but not the inactive structural analogue KN-92 were also effective. The calcineurin inhibitor deltamethrin did not interfere with CGS 21680. It is suggested that the transduction mechanism of A(2A) receptors to inhibit NMDA receptor channels is the phospholipase C/InsP(3)/calmodulin and calmodulin kinase II pathway. The adenylate cyclase/protein kinase A and phospholipase C/protein kinase C pathways do not appear to be involved.


Subject(s)
Brain/drug effects , N-Methylaspartate/pharmacology , Neurons/drug effects , Purinergic P1 Receptor Antagonists , alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology , Adenylyl Cyclases/metabolism , Animals , Brain/metabolism , Calcium-Calmodulin-Dependent Protein Kinases/metabolism , Calmodulin/metabolism , Cyclic AMP-Dependent Protein Kinases/metabolism , In Vitro Techniques , Inositol 1,4,5-Trisphosphate/metabolism , Neurons/metabolism , Protein Kinase C/metabolism , Rats , Rats, Wistar , Receptor, Adenosine A2A , Receptors, Purinergic P1/metabolism , Signal Transduction , Type C Phospholipases/metabolism , Visual Cortex/drug effects , Visual Cortex/metabolism
11.
Naunyn Schmiedebergs Arch Pharmacol ; 362(6): 568-76, 2000 Dec.
Article in English | MEDLINE | ID: mdl-11138850

ABSTRACT

The effect and mode of action of ethanol on N-methyl-D-aspartate (NMDA) and alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors of rat cortical neurons in primary culture were compared by means of the patch-clamp technique. The maxima of the concentration-response curves for both NMDA and AMPA were markedly depressed by ethanol without an apparent shift of the curves to the right. Ethanol inhibited the effects of NMDA and AMPA concentration-dependently and equally well. Excitatory amino acid (EAA) currents were depressed to the largest extent when ethanol was continuously superfused during and between agonist applications; a smaller inhibitory effect was observed when ethanol was intermittently superfused during agonist applications only. There was no inhibition by ethanol, when its superfusion was between two agonist applications. According to expectations, the analysis of the ratios of plateau to peak currents failed to suggest a use-dependent blockade by ethanol. In addition, comparison of the voltage-current curves of NMDA and AMPA in the absence and presence of ethanol indicated a voltage-independent effect and no change in the reversal potential of the two agonists. Finally, the measurement of activation and deactivation time constants for the ethanol-induced inhibition of NMDA and AMPA responses confirms the failure of ethanol to cause an open-channel block. In conclusion, these findings as a whole indicate that ethanol inhibits NMDA and AMPA currents by a noncompetitive mechanism. The mode of action appears to be similar for both NMDA and AMPA; under the conditions of the present study, a selective interference with structural motifs of the NMDA receptor is unlikely.


Subject(s)
Central Nervous System Depressants/pharmacology , Ethanol/pharmacology , Receptors, AMPA/antagonists & inhibitors , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Animals , Cells, Cultured , Cerebral Cortex/drug effects , Drug Interactions , Electric Stimulation , Excitatory Amino Acid Agonists/pharmacology , Membrane Potentials/drug effects , N-Methylaspartate/pharmacology , Neurons/drug effects , Patch-Clamp Techniques , Rats , alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid/pharmacology
12.
Neurochem Int ; 35(2): 153-62, 1999 Aug.
Article in English | MEDLINE | ID: mdl-10405999

ABSTRACT

Ethanol is a potent inhibitor of the N-methyl-D-aspartate (NMDA)-receptor subtype of glutamate receptor in a number of brain areas. The mechanism of ethanol action has been investigated by means of patch-clamp recording of ionic currents and fura-2 measurement of intracellular Ca2+ concentration in cell culture systems; the subunit composition of NMDA receptors and their influence on the effect of ethanol was determined by molecular biology methods. Ethanol does not appear to interact with NMDA either at the glutamate recognition site of the receptor, or at any of the hitherto known multiple modulatory sites, such as the glycine or polyamine site. Moreover, ethanol does not cause an open channel block by itself and fails to interact with Mg2+ at the site where it causes open channel block. The ability of ethanol to inhibit responses to NMDA is dependent on the subunit combination of NMDA receptors. The NR1/NR2A and NR1/NR2B combinations are preferentially sensitive to ethanol inhibition. Chronic treatment with ethanol leads to an increase of the NMDA receptor number at the transcriptional and posttranscriptional level; the receptor function is also facilitated. This causes withdrawal-type seizures after termination of chronic treatment with ethanol. The inhibition of NMDA receptors by ethanol leads to the depression of excitatory synaptic potentials mediated by this type of excitatory amino acid receptor. Ethanol-induced disturbances in certain regions of the brain, i.e. hippocampus, nucleus accumbens or locus coeruleus may lead to cognitive disorders or drug dependence. Brain slices containing the locus coeruleus may be used as an in vitro test system to investigate the addictive properties of ethanol.


Subject(s)
Ethanol/pharmacology , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Animals , Humans , Substance-Related Disorders
13.
Naunyn Schmiedebergs Arch Pharmacol ; 357(2): 186-9, 1998 Feb.
Article in English | MEDLINE | ID: mdl-9521493

ABSTRACT

Whole-cell patch clamp recordings were made in a pontine slice preparation of the rat brain containing the nucleus locus coeruleus (LC). In a first series of experiments, it was demonstrated that tyrosine hydroxylase-positive LC neurons of young (10-14 days of age) rats are multipolar with numerous dendrites. When pipettes filled with the marker molecule biocytin were used for recording, all cells exhibiting outward current responses to noradrenaline (100 microM) showed the morphology typical for LC neurons. At a holding potential of -80 mV, noradrenaline (100 microM) produced a comparably small outward current both in LC neurons of young (8-14 days) and older (18-23 days) rats. In contrast, 2-methylthio ATP (2-MeSATP; 100 microM) caused a relatively small inward current in the young animals, while inward current responses were much larger in most older animals (8 out of a total of 11). It is suggested that after birth there are probably no functional P2 purinoceptors present at LC neurons. Thereafter, P2 purinoceptor-function increases with age, reaching maturity only in animals older than 18 days.


Subject(s)
Aging/metabolism , Locus Coeruleus/metabolism , Neurons/metabolism , Receptors, Adrenergic, alpha-2/metabolism , Receptors, Purinergic P2/metabolism , Adenosine Triphosphate/analogs & derivatives , Adenosine Triphosphate/pharmacology , Adrenergic alpha-2 Receptor Agonists , Adrenergic alpha-Agonists/pharmacology , Animals , Female , Histocytochemistry , In Vitro Techniques , Injections, Intraventricular , Locus Coeruleus/cytology , Locus Coeruleus/drug effects , Male , Membrane Potentials/drug effects , Neurons/drug effects , Norepinephrine/pharmacology , Patch-Clamp Techniques , Purinergic P2 Receptor Agonists , Rats , Rats, Wistar , Thionucleotides/pharmacology
14.
Amino Acids ; 14(1-3): 33-9, 1998.
Article in English | MEDLINE | ID: mdl-9871438

ABSTRACT

Whole-cell patch clamp experiments were carried out in rat striatal brain slices. In a subset of striatal neurons (70-80%), NMDA-induced inward currents were inhibited by the adenosine A2A receptor selective agonist CGS 21680. The non-selective adenosine receptor antagonist 8-(p-sulphophenyl)-theophylline and the A2A receptor selective antagonist 8-(3-chlorostyryl)caffeine abolished the inhibitory action of CGS 21680. Intracellular GDP-beta-S, which is known to prevent G protein-mediated reactions, also eliminated the effect of CGS 21680. Extracellular dibutyryl cAMP, a membrane permeable analogue of cAMP, and intracellular Sp-cAMPS, an activator of cAMP-dependent protein kinases (PKA), both abolished the CGS 21680-induced inhibition. By contrast, Rp-cAMPS and PKI 14-24 amide, two inhibitors of PKA had no effect. Intracellular U-73122 (a phospholipase C inhibitor) and heparin (an inositoltriphosphate antagonist) prevented the effect of CGS 21680. Finally, a more efficient buffering of intracellular Ca2+ by a substitution of EGTA (11 mM) by BAPTA (5.5 mM) acted like U-73122 or heparin. Hence, A2A receptors appear to negatively modulate NMDA receptor channel conductance via the phospholipase C/inositoltriphosphate/Ca2+ pathway rather than the adenylate cyclase/PKA pathway.


Subject(s)
Neostriatum/physiology , Neurons/physiology , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors , Receptors, Purinergic P1/physiology , Adenosine/analogs & derivatives , Adenosine/antagonists & inhibitors , Adenylyl Cyclases/metabolism , Animals , Caffeine/analogs & derivatives , Caffeine/pharmacology , Cyclic AMP-Dependent Protein Kinases/metabolism , Enzyme Inhibitors/pharmacology , In Vitro Techniques , Neostriatum/drug effects , Neostriatum/enzymology , Neurons/drug effects , Neurons/enzymology , Patch-Clamp Techniques , Phenethylamines/antagonists & inhibitors , Purinergic P1 Receptor Agonists , Rats , Signal Transduction , Theophylline/analogs & derivatives , Theophylline/pharmacology
15.
Alcohol ; 14(5): 445-54, 1997.
Article in English | MEDLINE | ID: mdl-9305459

ABSTRACT

Chronic ethanol treatment of Wistar rats to 10% (v/v) ethanol over a period of 4, 12, and 36 weeks produced distinct alterations of the glial fibrillary acidic protein immunoreactivity (GFAP-IR) of dorsal hippocampal astrocytes. Ethanol consumption over a period of 4 weeks caused an increase in the total GFAP-IR of the astrocytes. Down-regulation of the total GFAP-IR was measured in all examined brain regions after 36 weeks of ethanol treatment. Prolonged ethanol treatment induced a significant loss of the total number of hippocampal pyramidal and dentate gyrus granule cells. Regional differences in the vulnerability to the neurotoxic effects of chronic ethanol intake over 36 weeks were found: CA3 > CA1 + CA2 > > CA4 > GD. In agreement with the degree of neuronal cell loss, ethanol-induced behavioral impairments were found. The acquisition of maze performance using a complex elevated labyrinth was deteriorated after 36 weeks of ethanol treatment, suggesting a deficit in learning and memory. These findings illustrate the importance of time-response analysis when determining the structural and functional changes produced by chronic ethanol treatment.


Subject(s)
Astrocytes/drug effects , Behavior, Animal/drug effects , Ethanol/pharmacology , Hippocampus/drug effects , Neurons/drug effects , Animals , Astrocytes/physiology , Behavior, Animal/physiology , Energy Intake , Ethanol/administration & dosage , Ethanol/blood , Glial Fibrillary Acidic Protein/analysis , Hippocampus/chemistry , Hippocampus/cytology , Immunohistochemistry , Male , Neurons/physiology , Rats , Rats, Wistar , Tissue Distribution
16.
Br J Pharmacol ; 122(1): 71-80, 1997 Sep.
Article in English | MEDLINE | ID: mdl-9298530

ABSTRACT

1. In order to investigate the modulatory effects of adenosine on excitatory amino acid projections onto striatal medium spiny neurons, whole-cell patch clamp experiments were carried out in rat brain slices. The effects of various agonists for P1 (adenosine) and P2 (ATP) purinoceptors and their antagonists were investigated. The A2A receptor agonist 2-p-(2-carboxyethyl)phenythylamino-5'-N-ethylcarboxamidoadenosine (CGS 21680; 0.1 microM), the A1 receptor agonist 2-chloro-N6-cyclcopentyladenosine (CCPA; 10 microM) and the non-selective P1 purinoceptor antagonist 8-(p sulphophenyl)-theophylline (8-SPT; 100 microM) did not alter the resting membrane potential, the threshold current necessary to elicit an action potential, the amplitude of spikes, their rise time, the amplitude of the afterhyperpolarization (AHP) and the time to peak of the AHP. 2. N-methyl-D-aspartate (NMDA; 1-1000 microM) caused a concentration-dependent inward current which was larger in the absence than in the presence of Mg2+ (1.3 mM). In a subset of striatal neurones, the current response to NMDA (10 microM) and the accompanying increase in conductance were both inhibited by CGS 21680 (0.01-1 microM). The effect of CGS 21680 (0.1 microM) persisted in the presence of tetrodotoxin (0.5 microM) or in a Ca(2+)-free medium, under conditions when synaptically mediated influences may be negligible. 3. The A3 receptor agonist N6-2-(4-aminophenyl)ethyladenosine (APNEA; 0.1-10 microM) also diminished the effect of NMDA (10 microM), while the A1 receptor agonists CCPA (0.1-10 microM) and (2S)-N6-[2-endonorbornyl] adenosine [S(-)-ENBA; 10 microM] as well as the endogenous, non-selective P1 purinoceptor agonist adenosine (100 microM) were inactive. The endogenous non-selective P2 purinoceptor agonist ATP (1000 microM) also failed to alter the current response to NMDA (10 microM). Adenosine (100 microM), but not ATP (1000 microM) became inhibitory after blockade of nucleoside uptake by S[4-nitrobenzyl)-6-thioguanosine (NBTG; 30 microM). 4. 8-(p-Sulphophenyl)-theophylline (8-SPT; 100 microM), as well as the A2A receptor antagonist 8-(3-chlorostyryl) caffeine (CSC; 1 microM) and the A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) at 0.03, but not 0.003 microM abolished the inhibitory action of CGS 21,680 (0.1 microM). None of these compounds altered the effect of NMDA (10 microM) by itself. DPCPX (0.03 microM) prevented the inhibition of APNEA (10 microM). 5. There was no effect of CGS 21,680 (0.1 microM), when guanosine 5'-O-(3-thiodiphosphate (GDP-beta-S; 300 microM) was included in the pipette solution in order to block G protein-mediated reactions. 6. In conclusion, adenosine receptors, probably of the A2A-subtype, inhibit the conductance of NMDA receptor channels in a subset of medium spiny neurones of the rat striatum by a transduction mechanism which involves a G protein.


Subject(s)
Adenosine/analogs & derivatives , Adenosine/pharmacology , Ion Channels/physiology , Neostriatum/drug effects , Neural Conduction/drug effects , Neurons/drug effects , Receptors, N-Methyl-D-Aspartate/physiology , Adenosine Triphosphate/pharmacology , Animals , Antihypertensive Agents/pharmacology , Carrier Proteins/antagonists & inhibitors , Carrier Proteins/metabolism , Cell Membrane/drug effects , Cell Membrane/physiology , Ion Channels/drug effects , Membrane Potentials/drug effects , Membrane Potentials/physiology , Membrane Proteins/antagonists & inhibitors , Membrane Proteins/metabolism , N-Methylaspartate/metabolism , N-Methylaspartate/pharmacology , Neostriatum/cytology , Neostriatum/physiology , Neurons/classification , Neurons/physiology , Nucleoside Transport Proteins , Phenethylamines/pharmacology , Purinergic P1 Receptor Agonists , Purinergic P1 Receptor Antagonists , Purinergic P2 Receptor Agonists , Rats , Rats, Wistar , Receptors, N-Methyl-D-Aspartate/drug effects , Receptors, N-Methyl-D-Aspartate/metabolism , Signal Transduction/drug effects , Signal Transduction/physiology
17.
Toxicol Appl Pharmacol ; 143(1): 83-8, 1997 Mar.
Article in English | MEDLINE | ID: mdl-9073595

ABSTRACT

The present study investigates the influence of long-term ethanol (ETOH) treatment of rats [10% (v/v) for 4, 12, and 36 weeks] on the metabolism of DCM after its oral and inhalative uptake to CO. Biotransformation of DCM to CO as measured by carboxyhemoglobin (COHb) formation was stimulated after long-term ETOH treatment in rats. A single oral dose of DCM (6.2 mmol/kg body mass) caused a significant increase of COHb, the maximum of about 9% occurring approximately 6 hr after DCM administration. In comparison to this control, in the blood of rats pretreated with ETOH (10% v/v) for 4, 12, and 36 weeks COHb values of 18, 17, and 13%, respectively, were measured. Long-term ETOH treatment followed by inhalation of 100, 500, and 2500 ppm DCM for 4 hr stimulated the formation of COHb, compared to controls. The elevation of COHb level was accompanied by decreased concentrations of DCM in the blood. The reason for the elevated biotransformation of DCM was ascertained by means of the determination of p-nitrophenol and aniline hydroxylation in liver microsomes of rats after long-term ETOH treatment to be an increase in cytochrome P450-dependent enzyme activities.


Subject(s)
Carbon Monoxide/pharmacokinetics , Central Nervous System Depressants/pharmacology , Ethanol/administration & dosage , Methylene Chloride/pharmacokinetics , Aniline Compounds/metabolism , Animals , Biotransformation/drug effects , Body Weight/drug effects , Carboxyhemoglobin/drug effects , Carcinogens/metabolism , Cytochrome P-450 CYP2E1/drug effects , Cytochrome P-450 CYP2E1/metabolism , Drug Administration Schedule , Energy Intake/drug effects , Liver/drug effects , Liver/pathology , Male , Methylene Chloride/toxicity , Microsomes, Liver/drug effects , Microsomes, Liver/metabolism , Nitrophenols/metabolism , Organ Size/drug effects , Rats , Rats, Wistar , Transferases/drug effects , Transferases/metabolism
18.
Alcohol ; 13(1): 69-74, 1996.
Article in English | MEDLINE | ID: mdl-8837938

ABSTRACT

The present study investigates the influence of long-term ethanol (EtOH) treatment of rats [10% (v/v) for 1, 4, 12, and 36 weeks] on hepatic microsomal cytochrome P450 (P450) content and liver aniline and p-nitrophenol hydroxylation. Total P450 per liver was stimulated after EtOH treatment for 1, 4, and 12 weeks. In the case of longer EtOH treatment no additional stimulation in P450 content was observed. Aniline and p-nitrophenol hydroxylase activity increased in direct relation with the duration of EtOH consumption. The stimulation of both enzymatic activities was different. In comparison to controls, in rats treated with 10% (v/v) EtOH for 1, 4, 12, and 36 weeks, an increase in nitrocatechol formation (1.1-, 1.2-, 2.2-, and 2.8-fold, respectively) was found. In contrast, no effect was observed on the metabolism of aniline after 1 and 4 weeks of EtOH consumption. Aniline hydroxylation increased after 12 and 36 weeks of EtOH treatment only. Addition of EtOH in vitro had an inhibitory effect on both aniline and p-nitrophenol hydroxylation. With liver microsomes from controls as well as EtOH-treated rats the inhibition of p-nitrophenol hydroxylation was competitive in nature (Ki = 5.6 mM and Ki = 5.9 mM). In contrast, there was a competitive inhibition of aniline hydroxylation with liver microsomes from controls only. With microsomes from EtOH-treated rats a mixed inhibition was found.


Subject(s)
Aniline Compounds/metabolism , Ethanol/pharmacology , Liver/drug effects , Nitrophenols/metabolism , Aniline Hydroxylase/metabolism , Animals , Cytochrome P-450 CYP2E1/metabolism , Cytochrome P-450 Enzyme System/metabolism , Ethanol/administration & dosage , Hydroxylation , Liver/metabolism , Male , Microsomes, Liver/drug effects , Microsomes, Liver/enzymology , Rats , Rats, Wistar
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