Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Tissue Eng Part C Methods ; 17(3): 367-74, 2011 Mar.
Article in English | MEDLINE | ID: mdl-20950252

ABSTRACT

Systematic advancements in the field of musculoskeletal tissue engineering require clear communication about the mechanical environments that promote functional tissue growth. To support the rapid discovery of effective mechanostimulation protocols, this study developed and validated a mechanoactive transduction and evaluation bioreactor (MATE). The MATE provides independent and consistent mechanical loading of six specimens with minimal hardware. The six individual chambers accurately applied static and dynamic loads (1 and 10 Hz) in unconfined compression from 0.1 to 10 N. The material properties of poly(ethylene glycol) diacrylate hydrogels and bovine cartilage were measured by the bioreactor, and these values were within 10% of the values obtained from a standard single-chamber material testing system. The bioreactor was able to detect a 1-day 12% reduction (2 kPa) in equilibrium modulus after collagenase was added to six collagenase sensitive poly(ethylene glycol) diacrylate hydrogels (p = 0.03). By integrating dynamic stimulation and mechanical evaluation into a single batch-testing research platform, the MATE can efficiently map the biomechanical development of tissue-engineered constructs during long-term culture.


Subject(s)
Bioreactors , Materials Testing/methods , Mechanical Phenomena , Tissue Engineering/instrumentation , Tissue Engineering/methods , Tissue Scaffolds/chemistry , Collagenases/metabolism , Hydrogels/chemistry , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL
...