Improving nitrogen retention of cattle slurry with oxidized biochar: An incubation study with three different soils.

The application of livestock slurry in soils can lead to nitrogen (N) losses through ammonia (NH3 ) emission or nitrate (NO3 - ) leaching. Oxidized biochar has great potential to mitigate N losses due to its strong adsorption capacity; however, the effects of oxidized biochar in different soils treated with slurry are currently unclear. Here, we investigated the effect of untreated and oxidized biochar (applied at a rate of 50 kg C m-3 slurry) on reducing N losses in a laboratory experiment with three different soils (loamy sand, sandy loam, loam) amended with cattle slurry at an application rate of 73 kg N ha-1 . Oxidized biochar reduced NH3 emissions by 64-75% in all soils, whereas untreated biochar reduced NH3 emissions by 61% only in the loamy sand. Oxidized biochar significantly reduced the NO3 - content in the soil solution of the loamy sand in the early phase of the incubation and led to a significantly higher NO3 - concentration in the same soil compared with the slurry-only treatment at the end of the experiment, indicating a significant increase in NO3 - retention in this organic C-poor soil. We conclude that oxidized biochar can reduce N losses, both in the form of NH3 emission and NO3 - leaching, from cattle slurry applied to soil, particularly in soil with soil organic carbon content <1% and pH <5 (i.e., oxidized biochar can serve as a means for improving the quality of marginal and acidic soils).

Hydroclimatic record from an Altiplano cushion peatland (24°S) indicates large-scale reorganisation of atmospheric circulation for the late Holocene.

The hydroclimate of South America is characterized by the South American summer monsoon (SASM), a tropical atmospheric circulation that induces a summer precipitation regime, and the Southern Hemisphere Westerlies (SHW), an extratropical atmospheric circulation that induces a winter precipitation regime. Stretched between these two systems is a NW-SE-oriented region dominated by descending air masses, resulting in the South American subtropical dry zone (SASDZ), also known as the arid diagonal. We investigated the Cerro Tuzgle cushion peatland (CTP) located on the Argentine Altiplano, north of the present-day SASDZ. Previous work revealed that the CTP was consistently in the SASM regime during the last 2900 cal yr BP. Here, we extend the CTP record to the middle Holocene covering the last 7200 cal yr BP to gain further knowledge of the Holocene development of the SASM and potential modulations of the SASDZ. The prominent feature of the entire record is a distinct and lasting transition centred around 3100 cal yr BP characterized by declining minerogenic content, increasing organic carbon content, rising stable carbon isotope values of organic matter and cellulose, and increasing stable oxygen isotope values of cellulose. We interpret this specific proxy pattern as a hydroclimatic transition towards less arid conditions at the CTP after 3100 cal yr BP. The transition corresponds with the end of the continuous Holocene strengthening of the SASM between 3500 cal yr BP and 3000 cal yr BP indicated by proxy records from north and east of the CTP. The CTP does not reflect this strengthening of the SASM and rather exhibits a threshold response indicating the effective establishment of the SASM summer precipitation regime at 24°S. This suggests that moisture supply during a more arid middle Holocene was provided by isotopically depleted precipitation, while moisture supply after the transition originated from isotopically enriched SASM summer precipitation. Concurrent hydroclimatic changes in the SHW winter precipitation regime south of the SASDZ are documented in a distinct lake level rise of Laguna Aculeo (33°50´S) around 3200 cal yr BP. These coinciding hydrological changes of the SASM and the SHW precipitation regimes indicate larger scale reorganisations of atmospheric circulation components, potentially connected to major modulations of the SASDZ. Thus, our CTP record sheds light on the middle to late Holocene development of the SASM at its southern limit and corroborates connections between the tropical and extratropical hydroclimate of South America.

Challenges in measuring nitrogen isotope signatures in inorganic nitrogen forms: An interlaboratory comparison of three common measurement approaches.

RATIONALE: Stable isotope approaches are increasingly applied to better understand the cycling of inorganic nitrogen (Ni ) forms, key limiting nutrients in terrestrial and aquatic ecosystems. A systematic comparison of the accuracy and precision of the most commonly used methods to analyze δ15 N in NO3 - and NH4 + and interlaboratory comparison tests to evaluate the comparability of isotope results between laboratories are, however, still lacking. METHODS: Here, we conducted an interlaboratory comparison involving 10 European laboratories to compare different methods and laboratory performance to measure δ15 N in NO3 - and NH4 + . The approaches tested were (a) microdiffusion (MD), (b) chemical conversion (CM), which transforms Ni to either N2 O (CM-N2 O) or N2 (CM-N2 ), and (c) the denitrifier (DN) methods. RESULTS: The study showed that standards in their single forms were reasonably replicated by the different methods and laboratories, with laboratories applying CM-N2 O performing superior for both NO3 - and NH4 + , followed by DN. Laboratories using MD significantly underestimated the "true" values due to incomplete recovery and also those using CM-N2 showed issues with isotope fractionation. Most methods and laboratories underestimated the at%15 N of Ni of labeled standards in their single forms, but relative errors were within maximal 6% deviation from the real value and therefore acceptable. The results showed further that MD is strongly biased by nonspecificity. The results of the environmental samples were generally highly variable, with standard deviations (SD) of up to ± 8.4‰ for NO3 - and ± 32.9‰ for NH4 + ; SDs within laboratories were found to be considerably lower (on average 3.1‰). The variability could not be connected to any single factor but next to errors due to blank contamination, isotope normalization, and fractionation, and also matrix effects and analytical errors have to be considered. CONCLUSIONS: The inconsistency among all methods and laboratories raises concern about reported δ15 N values particularly from environmental samples.

Low yield and abiotic origin of N2O formed by the complete nitrifier Nitrospira inopinata.

Nitrous oxide (N2O) and nitric oxide (NO) are atmospheric trace gases that contribute to climate change and affect stratospheric and ground-level ozone concentrations. Ammonia oxidizing bacteria (AOB) and archaea (AOA) are key players in the nitrogen cycle and major producers of N2O and NO globally. However, nothing is known about N2O and NO production by the recently discovered and widely distributed complete ammonia oxidizers (comammox). Here, we show that the comammox bacterium Nitrospira inopinata is sensitive to inhibition by an NO scavenger, cannot denitrify to N2O, and emits N2O at levels that are comparable to AOA but much lower than AOB. Furthermore, we demonstrate that N2O formed by N. inopinata formed under varying oxygen regimes originates from abiotic conversion of hydroxylamine. Our findings indicate that comammox microbes may produce less N2O during nitrification than AOB.

Potential of Wheat Straw, Spruce Sawdust, and Lignin as High Organic Carbon Soil Amendments to Improve Agricultural Nitrogen Retention Capacity: An Incubation Study.

Plants like winter wheat are known for their insufficient N uptake between sowing and the following growing season. Especially after N-rich crops like oilseed rape or field bean, nitrogen retention of the available soil N can be poor, and the risk of contamination of the hydrosphere with nitrate (NO3-) and the atmosphere with nitrous oxide (N2O) is high. Therefore, novel strategies are needed to preserve these unused N resources for subsequent agricultural production. High organic carbon soil amendments (HCA) like wheat straw promote microbial N immobilization by stimulating microbes to take up N from soil. In order to test the suitability of different HCA for immobilization of excess N, we conducted a laboratory incubation experiment with soil columns, each containing 8 kg of sandy loam of an agricultural Ap horizon. We created a scenario with high soil mineral N content by adding 150 kg NH4+-N ha-1 to soil that received either wheat straw, spruce sawdust or lignin at a rate of 4.5 t C ha-1, or no HCA as control. Wheat straw turned out to be suitable for fast immobilization of excess N in the form of microbial biomass N (up to 42 kg N ha-1), followed by sawdust. However, under the experimental conditions this effect weakened over a few weeks, finally ranging between 8 and 15 kg N ha-1 immobilized in microbial biomass in the spruce sawdust and wheat straw treatment, respectively. Pure lignin did not stimulate microbial N immobilization. We also revealed that N immobilization by the remaining straw and sawdust HCA material in the soil had a greater importance for storage of excess N (on average 24 kg N ha-1) than microbial N immobilization over the 4 months. N fertilization and HCA influenced the abundance of ammonia oxidizing bacteria and archaea as the key players for nitrification, as well as the abundance of denitrifiers. Soil with spruce sawdust emitted more N2O compared to soil with wheat straw, which in relation released more CO2, resulting in a comparable overall global warming potential. However, this was counterbalanced by advantages like N immobilization and mitigation of potential NO3- losses.

Interlaboratory assessment of nitrous oxide isotopomer analysis by isotope ratio mass spectrometry and laser spectroscopy: current status and perspectives.

RATIONALE: In recent years, research and applications of the N2O site-specific nitrogen isotope composition have advanced, reflecting awareness of the contribution of N2O to the anthropogenic greenhouse effect, and leading to significant progress in instrument development. Further dissemination of N2O isotopomer analysis, however, is hampered by a lack of internationally agreed gaseous N2O reference materials and an uncertain compatibility of different laboratories and analytical techniques. METHODS: In a first comparison approach, eleven laboratories were each provided with N2O at tropospheric mole fractions (target gas T) and two reference gases (REF1 and REF2). The laboratories analysed all gases, applying their specific analytical routines. Compatibility of laboratories was assessed based on N2O isotopocule data for T, REF1 and REF2. Results for T were then standardised using REF1 and REF2 to evaluate the potential of N2O reference materials for improving compatibility between laboratories. RESULTS: Compatibility between laboratories depended on the analytical technique: isotope ratio mass spectrometry (IRMS) results showed better compatibility for δ(15)N values, while the performance of laser spectroscopy was superior with respect to N2O site preference. This comparison, however, is restricted by the small number of participating laboratories applying laser spectroscopy. Offset and two-point calibration correction of the N2O isotopomer data significantly improved the consistency of position-dependent nitrogen isotope data while the effect on δ(15)N values was only minor. CONCLUSIONS: The study reveals that for future research on N2O isotopocules, standardisation against N2O reference material is essential to improve interlaboratory compatibility. For atmospheric monitoring activities, we suggest N2O in whole air as a unifying scale anchor.

Stable carbon isotope ratio analysis of anhydrosugars in biomass burning aerosol particles from source samples.

A new method for stable carbon isotope ratio analysis of anhydrosugars from biomass burning aerosol particle source filter samples was developed by employing Thermal Desorption--2 Dimensional Gas Chromatography--Isotope Ratio Mass Spectrometry (TD-2DGC-IRMS). Compound specific isotopic measurements of levoglucosan, mannosan, and galactosan performed by TD-2DGC-IRMS in a standard mixture show good agreement with isotopic measurements of the bulk anhydrosugars, carried out by Elemental Analyzer--Isotope Ratio Mass Spectrometry (EA-IRMS). The established method was applied to determine the isotope ratios of levoglucosan, mannosan, and galactosan from source samples collected during combustion of hard wood, softwood, and crop residues. δ(13)C values of levoglucosan were found to vary between -25.6 and -22.2‰, being higher in the case of softwood. Mannosan and galactosan were detected only in the softwood samples showing isotope ratios of -23.5‰ (mannosan) and -25.7‰ (galactosan). The isotopic composition of holocellulose in the plant material used for combustion experiments was determined with δ(13)C values between -28.5 and -23.7‰. The difference in δ(13)C of levoglucosan in biomass burning aerosol particles compared to the parent fuel holocellulose was found to be -1.89 (±0.37)‰ for the investigated biomass fuels. Compound specific δ(13)C measurements of anhydrosugars should contribute to an improved source apportionment.