ABSTRACT
Long-term minirhizotron observations of absorptive fine roots provide insights into seasonal patterns of belowground root production and carbon dynamics. Our objective was to compare root dynamics over time across mature individuals of 11 temperate trees species: five evergreen and six deciduous. We analyzed the timing and growth on 1st-and 2nd-order roots in minirhizotron images down to a vertical depth of 35 cm, as well as monthly and total annual length production. Production patterns were related to total annual precipitation of the actual and previous year of root production over 6 years. The main or largest peak of annual fine-root production occurred between June and September for almost all species and years. In most years, when peaks occurred, the timing of peak root production was synchronized across all species. A linear mixed model revealed significant differences in monthly fine-root length production across species in certain years (species x year, P < 0.0001), which was strongly influenced by three tree species. Total annual root production was much higher in 2000-2002, when there was above-average rainfall in the previous year, compared with production in 2005-2007, which followed years of lower-than-average rainfall (2003-2006). Compared to the wetter period all species experienced a decline of at least 75% in annual production in the drier years. Total annual root length production was more strongly associated with previous year's (P < 0.001) compared with the actual year's precipitation (P = 0.003). Remarkably similar timing of monthly absorptive fine-root growth can occur across multiple species of diverse phylogeny and leaf habit in a given year, suggesting a strong influence of extrinsic factors on absorptive fine-root growth. The influence of previous year precipitation on annual absorptive fine-root growth underscores the importance of legacy effects in biological responses and suggests that a growth response of temperate trees to extreme precipitation or drought events can be exacerbated across years.
ABSTRACT
⢠A wide variety of transparent materials are currently used for minirhizotron tubes. We tested the null hypothesis that minirhizotron composition does not influence root morphology and dynamics. ⢠Minirhizotron data were compared for glass, acrylic and butyrate tubes in apple (Malus domestica) and acrylic and butyrate tubes in a study with six forest tree species. ⢠Root phenology and morphology were generally similar among tubes. Apple root production was greatest against glass; these roots became pigmented later and lived longer than roots near acrylic or butyrate. Roots generally became pigmented faster next to butyrate than next to acrylic. Root survivorship was shorter near butyrate tubes in three of the four hardwood species; however, survivorship was shorter near acrylic tubes for the three conifer species. Comparison of minirhizotron standing crop data with root standing crop from cores showed that the acrylic data matched more closely than the butyrate data. ⢠This study reveals that the transparent material used often has little effect on root production but can substantially influence root survivorship in some plants.
