The seasonality of nutrients and sediment in residential stormwater runoff: Implications for nutrient-sensitive waters.

The discharge of excess nutrients to surface waters causes eutrophication, resulting in algal blooms, hypoxia, degraded water quality, reduced and contaminated fisheries, threats to potable water supplies, and decreases in tourism, cultural activities, and coastal economies. An understanding of the contribution of urban runoff to eutrophication is needed to inform management strategies. More broadly, the seasonality in nutrient concentrations and loads in urban runoff needs further analysis since algal blooms and hypoxia are seasonal in nature. This study quantifies the variation of nutrients and sediment in stormwater runoff across seasons from four urban residential sewersheds located in Columbus, Ohio, USA. An average of 62 runoff events at each sewershed were sampled using automated samplers during stormflow and analyzed for nutrients and total suspended solids (TSS). Spring total nitrogen concentrations had a significantly (p < 0.05) higher median concentration (2.19 mg/L) than fall (1.55 mg/L) and summer (1.50 mg/L). Total phosphorus concentrations were significantly higher in spring (0.22 mg/L) and fall (0.23 mg/L) than summer (0.15 mg/L). TSS concentrations were significantly higher in the spring (74.5 mg/L) and summer (56.5 mg/L) than the fall (34.0 mg/L). In contrast, seasonal loading differences for nutrients or sediment were rare because runoff volume varied in such a way as to offset significant concentration differences and significant seasonality in rainfall intensity. Annual pollutant loadings were similar in magnitude to other residential and even some agricultural runoff studies. Although nutrient loads are the key indicator for determining algal biomass, nutrient concentrations are important for real-time algal growth. Future research efforts should be focused not only on understanding how seasonal urban concentrations and loads impact coastal eutrophication, but also developing improved watershed management focused on critical periods. Improved designs for stormwater control measures need to account for seasonality in pollutant discharge.

Residential urban stormwater runoff: A comprehensive profile of microbiome and antibiotic resistance.

Non-point stormwater runoff is a major contamination source of receiving waterbodies. Heightened incidence of waterborne disease outbreaks related to recreational use and source water contamination is associated with extreme rainfall events. Such extreme events are predicted to increase in some regions due to climate change. Consequently, municipal separate storm sewer systems (MS4s) conveying pathogens to receiving waters are a growing public health concern. In addition, the spread of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria in various environmental matrices, including urban runoff, is an emerging threat. The resistome and microbiota profile of MS4 discharges has yet to be fully characterized. To address this knowledge gap, we first analyzed the relationship between rainfall depth and intensity and E. coli densities (fecal indicator) in stormwater from four MS4 outflows in Columbus, Ohio, USA during the spring and summer of 2017. Microbial source tracking (MST) was conducted to examine major fecal contamination sources in the study sewersheds. A subset of samples was analyzed for microbial and resistome profiles using a metagenomic approach. The results showed a significant positive relationship between outflow E. coli density and rainfall intensity. MST results indicate prevalent fecal contamination from ruminant populations in the study sites (91% positive among the samples tested). Protobacteria and Actinobacteria were two dominant bacteria at a phylum level. A diverse array of ARGs and potentially pathogenic bacteria (e.g. Salmonella enterica Typhimurium), fungi (e.g. Scedosporium apiospermum), and protists (e.g. Acanthamoeba palestinensis) were found in urban stormwater outflows that discharge into adjacent streams. The most prevalent ARGs among samples were ß-lactam resistance genes and the most predominant virulence genes within bacterial community were related with Staphylococcus aureus. A comprehensive contamination profile indicates a need for sustainable strategies to manage urban stormwater runoff amid increasingly intense rainfall events to protect public and environmental health.