ABSTRACT
Multiple signals are involved in the regulation of Ig production by human B lymphocytes. Leukotrienes, especially LTB4, have been shown to inhibit Ig production by increasing the number and function of suppressor lymphocytes. Production of leukotrienes has been demonstrated by mast cells, basophils, eosinophils, polymorphonuclear leukocytes, monocytes, and macrophages. In this paper we demonstrate that a human T-T hybridoma grown at 5 x 10(5) cells/ml constitutively produces 5 ng/ml of LTC4. Furthermore, we demonstrate that either the supernatant from this hybridoma containing 0.5 to 10 ng/ml LTC4 or purified LTC4 in the range of 0.5 to 5 ng/ml can suppress 50 to 70% of Ig production by unfractionated human mononuclear cells, by normal human cells stimulated with Staphylococcus aureus Cowan I and B cell differentiation factors, and by the EBV-transformed B cell line SKW.6 in the presence of B cell differentiation factors. Thus, LTC4 can have direct effects on B cells and may have a role in normal B cell regulation.
Subject(s)
Antibody-Producing Cells/metabolism , Hybridomas/metabolism , Immunoglobulins/biosynthesis , Immunosuppressive Agents/physiology , SRS-A/biosynthesis , T-Lymphocytes/metabolism , Antigens, Differentiation, T-Lymphocyte/analysis , Cell-Free System , Flow Cytometry , Humans , Hybridomas/analysis , Hybridomas/immunology , Immunosuppressive Agents/analysis , Immunosuppressive Agents/biosynthesis , SRS-A/isolation & purification , SRS-A/physiology , T-Lymphocytes/analysis , T-Lymphocytes/immunologyABSTRACT
To investigate possible etiologic factors for Reye's syndrome (RS), five survivors and their unaffected family members were studied. This study showed low salicylate levels among the patients with RS compared with siblings and parents when challenged with three doses of aspirin. Thus, the patients with RS, three to ten years after having had RS, exhibited normal or increased ability to metabolize aspirin. We found significantly higher antibody levels to influenza A and varicella among the patients with RS, further supporting the importance of these viral infections in the etiology of the syndrome.
Subject(s)
Antibodies, Viral/analysis , Reye Syndrome , Salicylates/metabolism , Adolescent , Adult , Child , Female , Follow-Up Studies , HLA Antigens/analysis , Herpesvirus 3, Human/immunology , Humans , Influenza A virus/immunology , Male , Psychological Tests , Reye Syndrome/complications , Reye Syndrome/genetics , Reye Syndrome/immunology , Reye Syndrome/metabolism , Salicylates/blood , Salicylic AcidABSTRACT
Antibody responses to JCV viral and T antigens were determined in owl monkeys following infection with JCV. Antibody to JCV virion antigen was present in all inoculated monkeys, arising within one months. These antibodies gradually diminished in all nontumor-bearing monkeys and in 10/13 tumor-bearing monkeys over the ensuing 12 months. Antibody to T antigen became evident later in most inoculated monkeys but had a marked variable course and did not correlate well with tumor production. The antibody patterns suggest that JCV is basically nonpermissive in owl monkeys and that the viral genome is retained in a prolonged persistent stage before progressing rapidly into a tumor.
