Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Plant Cell Rep ; 25(6): 582-91, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16456645

ABSTRACT

Production of transgenic organisms is a well-established, versatile course of action in molecular biology. Genetic engineering often requires heterologous, dominant antibiotic resistance genes that have been used as selectable markers in many species. However, as heterologous 5' and 3' flanking sequences often result in very low expression rates, endogenous flanking sequences, especially promoters, are mostly required and are easily obtained in model organisms, but it is much more complicated and time-consuming to get appropriate sequences from less common organisms. In this paper, we show that aminoglycoside 3'-phosphotransferase gene (aphVIII) based constructs with 3' and 5' untranslated flanking sequences (including promoters) from the multicellular green alga Volvox work in the unicellular green alga Chlamydomonas and flanking sequences from Chlamydomonas work in Volvox, at least if a low expression rate is compensated by an enforced high gene dosage. This strategy might be useful for all investigators that intend to transform species in which genomic sequences are not available, but sequences from related organisms exist.


Subject(s)
Chlamydomonas reinhardtii/genetics , Kanamycin Kinase/metabolism , Transformation, Genetic , Volvox/genetics , 3' Flanking Region , 5' Flanking Region , Animals , Chlamydomonas reinhardtii/enzymology , Cloning, Molecular , Gene Dosage , Genetic Markers , Kanamycin Kinase/genetics , Promoter Regions, Genetic , Volvox/enzymology
SELECTION OF CITATIONS
SEARCH DETAIL
...