ABSTRACT
Endogenous hyaluronidases in the human body have physiological and pathophysiological significance due to their enzymatic activity during the cleavage of hyaluronan. The potential of therapeutic use of testicular and recombinant hyaluronidase has been increasingly acknowledged and developed in recent years. Most relevant in practice is the co-application of hyaluronidase and other active substances in order to enhance their bioavailability within the tissue.
Subject(s)
Hyaluronoglucosaminidase/pharmacology , Pharmacokinetics , Skin Absorption/drug effects , Administration, Cutaneous , Animals , Biological Availability , Humans , Hyaluronic Acid/metabolism , Hyaluronoglucosaminidase/metabolism , Hyaluronoglucosaminidase/therapeutic useSubject(s)
Adjuvants, Anesthesia/administration & dosage , Anesthetics, Local/administration & dosage , Dermatologic Surgical Procedures , Hyaluronoglucosaminidase/administration & dosage , Lidocaine/administration & dosage , Adolescent , Adult , Anesthetics, Local/pharmacokinetics , Female , Humans , Injections, Subcutaneous , Lidocaine/pharmacokinetics , Male , Young AdultABSTRACT
BACKGROUND: Infiltration anesthesia of the skin is an analgesic procedure often practiced before minor surgical interventions or punctures. The addition of hyaluronidase is a possible option to improve the effectiveness of the local anesthetic with respect to expansion of effect. OBJECTIVE: To validate the safety of intracutaneous application of hyaluronidase as a lidocaine adjuvant. MATERIALS AND METHODS: The influence of adjuvant hyaluronidase on wound healing was investigated using the suction blister method in a prospective, single-center, placebo-controlled, double-blind, intraindividual comparison study with 20 participants. The target parameters were defined as the time of normalization of transepidermal water loss, hemovascular perfusion, and complete macroscopic epithelization of the wound. RESULTS: No evidence was found that adjuvant application of hyaluronidase retards wound healing. CONCLUSION: The addition of hyaluronidase to lidocaine in intracutaneous infiltration analgesia does not lead to retardation of wound healing, and no additional relevant risks were observed.
