Pre-Incisional and Multiple Intradermal Injection of N-Acetylcysteine Slightly Improves Incisional Wound Healing in an Animal Model.

The objective of this study was to investigate if delivering multiple doses of N-acetylcysteine (NAC) post-surgery in addition to pre-incisional administration significantly impacts the wound healing process in a rat model. Full-thickness skin incisions were carried out on the dorsum of 24 Sprague-Dawley rats in six locations. Fifteen minutes prior to the incision, half of the sites were treated with a control solution, with the wounds on the contralateral side treated with solutions containing 0.015%, 0.03% and 0.045% of NAC. In the case of the NAC treated group, further injections were given every 8 h for three days. On days 3, 7, 14 and 60 post-op, rats were sacrificed to gather material for the histological analysis, which included histomorphometry, collagen fiber organization analysis, immunohistochemistry and Abramov scale scoring. It was determined that scars treated with 0.015% NAC had significantly lower reepithelization than the control at day 60 post-op (p = 0.0018). Scars treated with 0.045% NAC had a significantly lower collagen fiber variance compared to 0.015% NAC at day 14 post-op (p = 0.02 and p = 0.04) and a lower mean scar width than the control at day 60 post-op (p = 0.0354 and p = 0.0224). No significant differences in the recruitment of immune cells and histological parameters were found. The results point to a limited efficacy of multiple NAC injections post-surgery in wound healing.

The impact of pesticides used at the agricultural land of the Puck commune on the environment of the Puck Bay.

BACKGROUND: The Puck commune is one of the largest agricultural regions in the Pomeranian Voivodship that due to the pollution of the coastal zone negatively affects the functioning of the Puck Bay, including health of its inhabitants, and causes decrease in tourism as well as in overall economic value of the region. The objective of the undertaken study was to assess the extent of risk to the environment posed by the pesticides used in agricultural production in the coastal area of the Puck commune. METHODS: The study focused on organochlorine insecticides (DDT and its metabolites: α, ß, ϒ, δ-HCH, aldrin, dieldrin, endrin, isodrine), glyphosate and its metabolite AMPA, and 309 active substances used as pesticides. Analyses were carried out using GC-MS, GC-MS/MS and LC-MS/MS techniques. The undertaken novel approach included "tracking" of a large number of substances in multiple environmental matrices (surface water, groundwater, seawater, soil, sediment and fish), along with examination of their transport routes from the pesticides application locality to the Puck Bay. RESULTS: Glyphosate and its metabolite AMPA, anthraquinone, boscalid, chlorpyrifos-ethyl, dimethachlor, diflufenican, difenoconazole, epoxiconazole, fluopicolide and metazachlor were found in soil samples and surface water samples collected from drainage ditches surrounding the studied agricultural plots. In the samples of seawater and fish taken from the Puck Bay no studied pesticides were found.