Investigation of Magnetic Electrodes in Conducting Polymeric Materials: Electrochemical Properties of a Fullerene[C60 ]-Pd Polymer and Iron Oxide Magnetic Nanocomposite.

A composite of iron oxide magnetic nanoparticles and coordination fullerene polymer (C60 Pd3 )n is formed by chemical deposition of spherical polymer nanoparticles on iron oxide magnetic nanoparticles in benzene containing C60 and Pd(0) complex. The composition of the composite can be controlled by the amount of magnetite and concentration of polymerization precursors as well as the time of polymerization. The magnetic composite material Fe3 O4 -γFe2 O3 /(C60 Pd3 )n is used as a model system to investigate its deposition on a magnetic electrode and its electrochemical properties. The iron oxide magnetic nanoparticles ensure both the magnetic activity of the composite and its nanostructured morphology. Both of these factors are responsible for the enhancement of the electrochemical activity of the polymer phase forming the composite in comparison to the pure polymer material deposited on the same magnetic electrode. In the magnetic field of the electrode, the composite undergoes permanent and strong bonding with the surface of the electrode. The nanostructured morphology of the Fe3 O4 -γFe2 O3 /(C60 Pd3 )n composite also provides very good capacitive properties.

Development of an SPR imaging biosensor for determination of cathepsin G in saliva and white blood cells.

Cathepsin G (CatG) is an endopeptidase that is associated with the early immune response. The synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of CatG via a new surface plasmon resonance imaging assay. CGI-I was immobilized on the gold surface of an SPR sensor that was first modified with 1-octadecanethiol. A concentration of CGI-I equal to 4.0 µg·mL-1 and a pH of 8.0 were found to give the best results. The dynamic response of the sensor ranges from 0.25 to 1.5 ng·mL-1, and the detection limit is 0.12 ng·mL-1. The sensor was applied to detect CatG in human saliva and white blood cells.FigureThe synthetic compound cathepsin G inhibitor I (CGI-I) was tested for its ability to inhibit the activity of cathepsin G via a newly developed surface plasmon resonance imaging assay. The sensor was applied to detect cathepsin G in human saliva and white blood cells.