ABSTRACT
The content of flavonoids in the flowers and leaves of Prunus spinosa L. was determined by spectrophotometric and RP-HPLC method. Determinations included hydrolysis of flavonoid glycosides in extracts from raw materials and then quantitative analysis of the obtained aglycones. Results were calculated for the content of glycosides and statistical analysis of the obtained data was performed.
Subject(s)
Flavonoids/analysis , Plants, Medicinal/chemistry , Prunus/chemistry , Rosaceae/chemistry , Calibration , Chromatography, High Pressure Liquid , Plant Leaves/chemistryABSTRACT
From the flowers of Prunus spinosa L. a mixture of alpha- and beta-amyrine, a mixture of ursolic and oleanolic acids, ursolic acid, a mixture of beta-sitosterol, gamma-sitosterol and stigmasterol and beta-sitosterol 3-O-beta-D-glucopyranoside were isolated. Their structure was determined by spectroscopic methods (GC/MS, IR, 1H and 13C NMR). Chromatographic methods (GC, HPLC, TLC) were employed to determine the presence of the listed compounds in the leaves. Also, the content of beta-sitosterol, ursolic and oleanolic acids in the leaves and flowers of blackthorn was determined by GC/MSD method.
Subject(s)
Phytosterols/analysis , Prunus/chemistry , Triterpenes/analysis , Phytosterols/isolation & purification , Plant Extracts/analysis , Plant Extracts/isolation & purification , Plant Leaves/chemistry , Triterpenes/isolation & purificationABSTRACT
Eight flavonoids were isolated from the flowers of Prunus spinosa: kaempferol, quercetin, kaempferol 3-O-alpha-L-arabinofuranoside, quercetin 3-O-alpha-L-arabinofuranoside, kaempferol 3-O-alpha-L-ramnopyranoside, kaempferol 7-O-alpha-L-ramnopyranoside, kaempferol 3-O-beta-D-xylopyranoside, kaempferol 3-O-(2''-E-p-coumaroyl)-alpha-L-arabinofuranoside. The last three have been found for the first time in this plant. The structure of the compounds was determined by means of chemical and spectral methods (UV, IR, LSI MS, 1H NMR, 13C NMR).
Subject(s)
Flavonoids/analysis , Kaempferols , Prunus/chemistry , Quercetin/analogs & derivatives , Chromatography, Liquid , Quercetin/chemistry , Quercetin/isolation & purificationABSTRACT
Ultrasound-induced oxidation of some flavonoids was detected by UV spectral analysis. From among twenty seven compounds investigated only flavonol aglycones and their 7-glycosides, but not 3-glycosides, flavones, catechins and flavanones were found to be oxidized with opening of the ring C. The products of quercetin oxidation were identical with those obtained during slow aerial oxidation of the compound in neutral solution. The oxidation products were deprived of the oxido-reductive properties of native flavonoids. Neither they reduced cytochrome c, nor inhibited lipid oxidation nor scavenged superoxide anions.
Subject(s)
Antioxidants/chemistry , Flavonoids/chemistry , Antioxidants/pharmacology , Cytochrome c Group/chemistry , Flavonoids/pharmacology , Indicators and Reagents , Lipids/chemistry , Mass Spectrometry , Oxidation-Reduction , Spectrophotometry, Ultraviolet , UltrasonicsABSTRACT
Thirty nine flavonoids, isolated from plants, were tested in respect of their influence on soybean lipoxygenase activity, cyclooxygenase activity and inhibition of ascorbic acid-stimulated malonaldehyde formation in liver lipids. Almost all of the tested compounds were antioxidants and stimulated cyclooxygenase when arachidonic acid was used as a substrate at a concentration of 100 microM. Eleven flavonoids were inhibitors of soybean lipoxygenase. A good correlation between the chemical structure and the tested activity was observed. The most active compounds in all tests were luteolin, 6-hydroxyluteolin, nepetin, quercetagetin, patuletin and myricetin.
