ABSTRACT
The examinations covered 255 workers of two polyvinyl chloride processing plants. All workers were subject to general medical examinations including electrocardiography. In a part of workers employed in the plant located in the Katowice Voivodeship, the dermatological tests and neurological examinations including testing the velocity of conduction in motor nerves were carried out and the selected group was subject also to capillaroscopy and dermatothermometry. In the examined workers no "vinyl chloride disease" was found; capillaroscopy and neurological examinations did not reveal any evident changes. However, some dermatoses, most frequently of allergic type, were often encountered.
Subject(s)
Air Pollutants, Occupational/toxicity , Chemical Industry , Polyvinyl Chloride/toxicity , Polyvinyls/toxicity , Capillaries/physiopathology , Heart/physiopathology , Humans , Median Nerve/physiopathology , Nails/blood supply , Neural Conduction/drug effects , Occupations , Peroneal Nerve/physiopathology , Tibial Nerve/physiopathology , Ulnar Nerve/physiopathologyABSTRACT
Various steps of the ninhydrin-reaction in the octanolic milieu (Wolman 1971; Pfister and Görne 1978) were tested on the cerebellum of adult rats. The results of the fluorescence histochemical procedure was found to be in comparison with the results ascertained to spectrofluorometric method. The findings were further indicative for a GABA-specificity of the reaction.
Subject(s)
Cerebellum/analysis , gamma-Aminobutyric Acid/analysis , Animals , Cerebellum/cytology , Microscopy, Fluorescence , Ninhydrin , Octanols , RatsABSTRACT
As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelate-complex, was formed. Partial reaction steps were investigated spectrofluorometrically. The excitation and emmission spectra resulting from the reaction between ninhydrin and glutamic acid, ninhydrin and gamma-aminobutyric acid, and ninhydrin, glutamic acid and gamma-aminobutyric acid were measured. Possible consequences are discussed with respect to fluorescence histochemical procedures.
Subject(s)
gamma-Aminobutyric Acid/analysis , Copper , Glutamates/analysis , Indicators and Reagents , Ninhydrin , Octanols , Spectrometry, Fluorescence/methodsABSTRACT
As a result of the reaction of ninhydrin with gamma-aminobutyric acid and glutamic acid in the octanolic milieu, a fluorescent product, presumably a copper-II-chelat-complex, was formed. This appears analogous to the fluorescence histochemical procedure. the reaction product displays the following spectrofluorometric properties: excitation peak at lambda = 375 nm and emission plaximum at lambda = 445 nm. Spectrofluorometric estimations during performing various steps of the reaction were indicative of possibilities of the improvement of the fluorescence histochemical GABA demonstration.
Subject(s)
gamma-Aminobutyric Acid/analysis , Glutamates , Ninhydrin , Octanols , Solvents , Spectrometry, FluorescenceSubject(s)
Lipids/blood , Pre-Eclampsia/blood , Pregnancy , Dietary Fats/administration & dosage , Female , HumansABSTRACT
During a 3-year period of treatment with DPH three episodes of drug intolerance developed in a 16-year-old epileptic girl. The dose tolerated previously was 200 mg daily. The authors stress the effect of hormonal disturbances on the change in DPH tolerance.
Subject(s)
Epilepsy/drug therapy , Phenytoin/toxicity , Puberty , Adolescent , Drug Tolerance , Female , Humans , Meniere Disease/chemically induced , Nystagmus, Pathologic/chemically induced , Phenytoin/therapeutic use , Vertigo/chemically inducedABSTRACT
The influence of intraventricular injection of 50 microng of noradrenaline (NA) on behavior of female Wistar rats in various phases of the sexual cycle, and the influence of 17-beta-estradiol and progesterone in castrated female rats were studied. Changes in motor and exploratory activity were observed in the course of the sexual cycle in female rats. Sensitivity to NA injected intraventricular varied significantly in different phases of the sexual cycle. Castration depressed the action of NA on motor and exploratory activities in rats, and progesterone prevented the depressive effect of NA in castrated rats.
Subject(s)
Behavior, Animal/drug effects , Estrus/drug effects , Norepinephrine/pharmacology , Animals , Castration , Cerebral Ventricles , Diestrus/drug effects , Estradiol/pharmacology , Exploratory Behavior/drug effects , Female , Injections, Spinal , Motor Activity/drug effects , Norepinephrine/administration & dosage , Pregnancy , Proestrus/drug effects , Progesterone/pharmacology , RatsABSTRACT
In male Wistar rats PGF2alpha or PGE1 were injected intracerebroventricularly (icv) in a dose of 1 or 10 mug. Immediately or 1 hr after injection the locomotor and exploratory activity were measured. The levels of noradrenaline (NA), dopamine (DA) 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and acetylcholine (Ach) were measured in discrete brain areas. Both PGs applied ivc caused the depression of locomotor and exploratory activity in rats. PGE1 acted longer. Both substances but PGE1 more intensively affected the level of estimated biogenic amines in different brain structures. It is concluded that PGF2alpha and E1 are central nervous depressants. Both PGs affect neurons producing NA or 5-HT or Ach in discrete areas of brain in different manner. There is different susceptibility of brain structures on PGs action.
