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1.
J Microbiol ; 59(7): 675-680, 2021 Jul.
Article in English | MEDLINE | ID: mdl-34061338

ABSTRACT

Sphingorhabdus sp. YGSMI21, a novel microbial strain with an enantioselective epoxide hydrolase activity, was isolated from tidal samples contaminated by accidental oil spills subjected to enriched culture with polycyclic aromatic hydrocarbon. This strain was able to optically decompose (R)-styrene oxide (SO) and showed 100% optical purity. In addition, it showed a good enantioselectivity for the derivatives of (S)-SO, (S)-2-chlorostyrene oxide (CSO), (S)-3-CSO and (S)-4-CSO. For (S)-2-CSO, (S)-3-CSO and (S)-4-CSO, 99.9%ee was obtained with the yield of 26.2%, 24.8%, and 11.0%, respectively, when using 10 mg cells of Sphingorhabdus sp. YGSMI21 at pH 8.0 with 4 mM racemic substrates at pH 8.0 and 25°C. The values obtained in this study for (S)-2-CSO, particularly the yield of 26.2%, is noteworthy, considering that obtaining an enantiomerically pure form is difficult. Taken together, Sphingorhabdus sp. YGSMI21 can be regarded as a whole-cell biocatalyst in the production of various (S)-CSO with the chlorine group at a different position.


Subject(s)
Epoxide Hydrolases/metabolism , Epoxy Compounds/metabolism , Geologic Sediments/microbiology , Sphingomonadaceae/isolation & purification , Hydrolysis , Sphingomonadaceae/classification , Sphingomonadaceae/enzymology , Stereoisomerism
2.
Genome Announc ; 6(3)2018 Jan 18.
Article in English | MEDLINE | ID: mdl-29348337

ABSTRACT

Sphingorhabdus sp. YGSMI21 is a novel strain exhibiting high enantioselective hydrolysis activity for styrene oxide. Here, we present its complete genome sequence, consisting of one circular chromosome (3.86 Mb) and one plasmid (0.196 Mb).

3.
Genome Announc ; 5(45)2017 Nov 09.
Article in English | MEDLINE | ID: mdl-29122863

ABSTRACT

Celeribacter sp. strain TSPH2, a novel producer of indigo, was isolated from oil-contaminated sediment. We present here its genome sequence consisting of one circular chromosome (4 Mb) and one plasmid (0.15 Mb), with an overall G+C content of 60.9%. This strain contains oxygenase genes involved in indigo synthesis, such as flavin-containing monooxygenase.

4.
Cancer Immunol Res ; 4(10): 823-834, 2016 10.
Article in English | MEDLINE | ID: mdl-27485136

ABSTRACT

Human papillomavirus (HPV), particularly HPV16 and HPV18, can cause cancers in diverse anatomical sites, including the anogenital and oropharyngeal (throat) regions. Therefore, development of safe and clinically effective therapeutic vaccines is an important goal. Herein, we show that a recombinant fusion protein of a humanized antibody to CD40 fused to HPV16.E6/7 (αCD40-HPV16.E6/7) can evoke HPV16.E6/7-specific CD8+ and CD4+ T-cell responses in head-and-neck cancer patients in vitro and in human CD40 transgenic (hCD40Tg) mice in vivo The combination of αCD40-HPV16.E6/7 and poly(I:C) efficiently primed HPV16.E6/7-specific T cells, particularly CD8+ T cells, in hCD40Tg mice. Inclusion of montanide enhanced HPV16.E6/7-specific CD4+, but not CD8+, T-cell responses. Poly(I:C) plus αCD40-HPV16.E6/7 was sufficient to mount both preventative and therapeutic immunity against TC-1 tumors in hCD40Tg mice, significantly increasing the frequency of HPV16-specific CD8+ CTLs in the tumors, but not in peripheral blood. In line with this, tumor volume inversely correlated with the frequency of HPV16.E6/7-specific CD8+ T cells in tumors, but not in blood. These data suggest that CD40-targeting vaccines for HPV-associated malignancies can provide a highly immunogenic platform with a strong likelihood of clinical benefit. Data from this study offer strong support for the development of CD40-targeting vaccines for other cancers in the future. Cancer Immunol Res; 4(10); 823-34. ©2016 AACR.


Subject(s)
CD40 Antigens/immunology , CD8-Positive T-Lymphocytes/immunology , Cancer Vaccines/immunology , Head and Neck Neoplasms/immunology , Papillomavirus Vaccines/immunology , Animals , Antiviral Agents/immunology , CD4-Positive T-Lymphocytes/immunology , Female , Human papillomavirus 16/immunology , Humans , Immunity, Cellular , Mice, Inbred C57BL , Mice, Transgenic , Poly I-C/immunology , Recombinant Fusion Proteins/immunology , Tumor Cells, Cultured , Uterine Cervical Neoplasms/prevention & control , Uterine Cervical Neoplasms/virology , Xenograft Model Antitumor Assays
5.
J Sci Food Agric ; 95(9): 1830-7, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25142414

ABSTRACT

BACKGROUND: Brown algae have been used for their nutritional value as well as a source of bioactive compounds with antioxidant, anti-inflammatory, antimicrobial and anti-obesity effects. Obesity is an important condition implicated in various diseases, including diabetes, hypertension, dyslipidemia and coronary heart disease. However, anti-obesity effects of Eisenia bicyclis remain unknown. RESULTS: We investigated the anti-obesity effects of 6,6'-bieckol, 6,8'-bieckol, 8,8'-bieckol, dieckol and phlorofucofuroeckol A isolated from E. bicyclis. Anti-obesity activity was evaluated by examining the inhibition of differentiation of 3T3-L1 adipocytes and the expression of peroxisome proliferator-activated receptor γ (PPARγ), CCATT/enhancer-binding protein α (C/EBPα) and sterol regulatory element binding protein-1c (SREBP-1c) at the mRNA and protein level. Differentiated 3T3-L1 cells were treated with the purified phlorotannins at concentrations of 10, 25 and 50 µg mL(-1) for 8 days. The results indicated that the purified phlorotannins suppressed the differentiation of 3T3-L1 adipocytes in a dose-dependent manner, without toxic effects. Among the five compounds, 6,6'-bieckol markedly decreased lipid accumulation and expression levels of PPARγ, C/EBPα, SREBP-1c (mRNA and protein), and fatty acid synthase and acyl-coA carboxylase (mRNA). CONCLUSION: These findings suggest that E. bicyclis suppressed differentiation of 3T3-L1 adipocyte through downregulation of adipogenesis and lipogenesis.


Subject(s)
Adipogenesis/drug effects , Anti-Obesity Agents/pharmacology , Dioxins/pharmacology , Down-Regulation/drug effects , Lipid Metabolism/drug effects , 3T3-L1 Cells , Animals , Anti-Obesity Agents/adverse effects , Anti-Obesity Agents/chemistry , Anti-Obesity Agents/isolation & purification , Benzofurans/adverse effects , Benzofurans/chemistry , Benzofurans/isolation & purification , Benzofurans/pharmacology , Carbon-Carbon Ligases/antagonists & inhibitors , Carbon-Carbon Ligases/genetics , Carbon-Carbon Ligases/metabolism , Cell Survival/drug effects , Dioxins/adverse effects , Dioxins/chemistry , Dioxins/isolation & purification , Fatty Acid Synthase, Type I/antagonists & inhibitors , Fatty Acid Synthase, Type I/genetics , Fatty Acid Synthase, Type I/metabolism , Mice , Molecular Structure , PPAR gamma/antagonists & inhibitors , PPAR gamma/genetics , PPAR gamma/metabolism , Pacific Ocean , Phaeophyceae/chemistry , Republic of Korea , Seaweed/chemistry , Stereoisomerism
6.
Int J Syst Evol Microbiol ; 64(Pt 4): 1351-1358, 2014 Apr.
Article in English | MEDLINE | ID: mdl-24436065

ABSTRACT

Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026(T) and MEBiC 08903(T), were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with [Cytophaga] fermentans DSM 9555(T). 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA-DNA relatedness between the two were 31.3-31.8 %. Phylogenetic analysis revealed that the two isolates and [Cytophaga] fermentans DSM 9555(T) were affiliated with the family Marinilabiliaceae in the class Bacteroidia. The dominant fatty acids of strains MEBiC 07026(T), MEBiC 08903(T) and [Cytophaga] fermentans DSM 9555(T) were branched-type or hydroxylated C15 : 0, but [Cytophaga] fermentans DSM 9555(T) contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family Marinilabiliaceae. The major respiratory quinone of the strains was MK-7. Strains MEBiC07026(T) and MEBiC08903(T) utilized a wide range of carboxylic acids whereas [Cytophaga] fermentans DSM 9555(T) utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of [Cytophaga] fermentans DSM 9555(T) revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, Carboxylicivirga gen. nov., is proposed in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. with type strain MEBiC 07026(T) ( = KCCM 42978(T) = JCM 18290(T)) and Carboxylicivirga taeanensis sp. nov. with type strain MEBiC 08903(T) ( = KCCM 43024(T) = JCM 19490(T)). Additionally, [Cytophaga] fermentans DSM 9555(T) ( = ATCC 19072(T)) is reclassified as Saccharicrinis fermentans gen. nov., comb. nov.


Subject(s)
Bacteroidetes/classification , Cytophaga/classification , Phylogeny , Seawater/microbiology , Bacterial Typing Techniques , Bacteroidetes/genetics , Bacteroidetes/isolation & purification , Base Composition , DNA, Bacterial/genetics , Fatty Acids/chemistry , Geologic Sediments/microbiology , Molecular Sequence Data , Nucleic Acid Hybridization , Phosphatidylethanolamines/chemistry , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistry
7.
Biotechnol Lett ; 36(2): 357-62, 2014 Feb.
Article in English | MEDLINE | ID: mdl-24129951

ABSTRACT

(S)-Styrene oxide, (S)-2-chlorostyrene oxide (CSO), (S)-3-CSO and (S)-4-CSO with 99.9 %ee were obtained with a yield of 20.6, 39.3, 28.7 and 26.8 % from 4 mM corresponding racemic substrates using 10 mg cells of a newly-isolated Sphingopyxis sp. at pH 8.0 and 25 °C in 1 ml 100 mM Tris/HCl buffer after 420, 100, 120 and 55 min, respectively. For racemic 2CSO, well-known for one of the racemates that is difficult to obtained in enantiomerically pure form, (S)-2-CSO with 99.9 %ee, 39.3 % yield (theoretical yield 50 %) and enantiomeric ratio of 42.1 was obtained. The newly-isolated strain can thus be used as whole-cell biocatalyst in the production of various (S)-CSO with a chlorine group at different positions.


Subject(s)
Epoxide Hydrolases/metabolism , Epoxy Compounds/metabolism , Sphingomonadaceae/enzymology , Sphingomonadaceae/metabolism , Buffers , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Hydrogen-Ion Concentration , Hydrolysis , Molecular Sequence Data , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sphingomonadaceae/classification , Sphingomonadaceae/isolation & purification , Temperature
8.
Adv Healthc Mater ; 2(5): 736-44, 2013 May.
Article in English | MEDLINE | ID: mdl-23184611

ABSTRACT

Prostate specific membrane antigen (PSMA) is overexpressed on prostate tumor cells and the neovascular endothelia various solid tumors. A bivalent immunotoxin generated by fusing a fold-back single-chain diabody derived from the Fv fragments of an anti-PSMA monoclonal antibody with a truncated diphtheria toxin (DT) containing the activity and translocation domains [A-dmDT390-scfbDb(PSMA)] might be suitable for targeted therapy of tumors that overexpress PSMA. In this study, a PSMA-positive and a PSMA-negative prostate cancer cell lines were treated with immunotoxin A-dmDT390-scfbDb(PSMA) in order to study the tumor targeting specificity and therapeutic potential of the immunotoxin. The cellular uptake and selective toxicity of the immunotoxin were evident in monolayer cultures of PSMA-positive LNCaP prostate cancer cells but not in cultures of PSMA-negative PC-3 prostate cancer cells. Cellular accumulation of A-dmDT390-scfbDb(PSMA) increased with increasing incubation times and concentrations in LNCaP cells. The proportion of apoptotic LNCaP cells increased upon incubation with increasing doses of the fold-back immunotoxin. Optical imaging and MRI with the Alexa Fluor 680-labeled A-dmDT390-scfbDb(PSMA) confirmed the specific targeting and therapeutic efficacy of this immunotoxin towards PSMA-positive LNCaP solid tumor xenografts in athymic nude mice.


Subject(s)
Immunotoxins/immunology , Immunotoxins/therapeutic use , Microscopy, Fluorescence/methods , Prostate-Specific Antigen/antagonists & inhibitors , Prostate-Specific Antigen/immunology , Prostatic Neoplasms/drug therapy , Prostatic Neoplasms/immunology , Cell Line, Tumor , Fluorescent Dyes , Humans , Male , Prostatic Neoplasms/pathology
9.
Biotechnol Lett ; 35(4): 599-606, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23242500

ABSTRACT

A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its derivatives, such as 2,3- and 4-chlorostyrene oxides, as a sole carbon source. Gordonia sp. H37 exhibited high enantioselective hydrolysis activity for 4-chlorostyrene oxide with an enantiomeric ratio of 27. Gordonia sp. H37 preferentially hydrolyzed the (R)-enantiomer of styrene oxide derivatives resulting in the preparation of a (S)-enantiomer with enantiomeric excess greater than 99.9 %. The enantioselective EHase activity was identified and characterized in various PAH-degrading bacteria, and whole cell Gordonia sp. H37 was employed as a biocatalyst for preparing enantiopure (S)-styrene oxide derivatives.


Subject(s)
Bacteria/enzymology , Bacteria/metabolism , Epoxide Hydrolases/metabolism , Epoxy Compounds/metabolism , Bacteria/isolation & purification , Carbon/metabolism , Geologic Sediments/microbiology , Hydrolysis
10.
Anticancer Agents Med Chem ; 11(10): 983-92, 2011 Dec.
Article in English | MEDLINE | ID: mdl-22023048

ABSTRACT

Tumor growth depends upon access to host blood vessels. Many steps in tumor angiogenesis have been defined including tumor cell hypoxia, tumor cell secretion of pro-angiogenic growth factors, receptor activation on host endothelium and stroma, and establishment of new blood vessels feeding the tumor mass. Inhibitors for some of these steps have been synthesized and tested clinically. While modest improvements in response, progression-free survival and overall survival have been observed in metastatic colorectal carcinoma, non-small cell lung carcinoma, breast carcinoma, renal cell carcinoma, and glioblastoma, almost all patients ultimately relapse and die from metastatic disease. Explanations for the limited effects of anti-angiogenesis therapy include lack of activity on all the parallel angiogenic pathways, non-specific toxicities of some of the agents, induction of a pro-metastatic phenotype by the enhanced hypoxia from therapy, and lack of effect on already established tumor blood vessels. One solution is to directly attack the tumor vasculature rather than inhibit tumor vessel formation. The flavonoid ASA404 and the tubulin-binder combretastatin A-4 phosphate directly damage tumor endothelium by different mechanisms. Both compounds have shown minimal single agent disease activity and produce cardiac ischemia in clinical trials. Recently, ligand-directed vascular disrupting agents have been synthesized and tested. A promising member of this class of therapeutics targets the tumor endothelial marker-8 (TEM8). Anti-TEM8 antibody drug conjugate may facilitate selective destruction of tumor blood vessels yielding enhanced anti-cancer efficacy and reduced normal tissue toxicities. Advances in this field are described which should lead to clinical studies of TEM8 targeted cancer therapeutics.


Subject(s)
Angiogenesis Inhibitors/therapeutic use , Drug Delivery Systems/methods , Immunoconjugates/therapeutic use , Neoplasm Proteins/immunology , Neoplasms/blood supply , Neoplasms/drug therapy , Receptors, Cell Surface/immunology , Amino Acid Sequence , Angiogenesis Inhibitors/immunology , Animals , Humans , Immunoconjugates/immunology , Microfilament Proteins , Models, Molecular , Molecular Sequence Data , Neoplasm Proteins/chemistry , Neovascularization, Pathologic/drug therapy , Receptors, Cell Surface/chemistry
11.
J Biosci Bioeng ; 110(3): 295-7, 2010 Sep.
Article in English | MEDLINE | ID: mdl-20547378

ABSTRACT

Previously we reported that an epoxide hydrolase (EHase) from Novosphingobium aromaticivorans could preferentially hydrolyze (R)-styrene oxide. In this study, we demonstrate that the purified NEH could be also effective in chiral resolution of racemic epichlorohydrin (ECH). Particularly, the purified NEH showed excellent hydrolyzing activity toward ECH to complete the reaction at a short period of incubation time. Enantiopure (S)-ECH could be obtained with a high enantiopurity of more than 99.99% enantiomeric excess (ee) and yield of 20.7% (theoretical, 50%). The chiral resolution of the purified NEH toward ECH was not susceptible to substrate inhibition by 500 mM racemic ECH.


Subject(s)
Alphaproteobacteria/enzymology , Epichlorohydrin/chemical synthesis , Epoxide Hydrolases/chemistry , Epoxy Compounds/chemistry , Stereoisomerism
12.
J Biosci Bioeng ; 109(6): 539-44, 2010 Jun.
Article in English | MEDLINE | ID: mdl-20471590

ABSTRACT

As a continuous effort of developing highly enantioselective epoxide hydrolase from marine microorganisms, it was found that Maritimibacter alkaliphilus KCCM 42376 [corrected] was highly enantioselective toward racemic glycidyl phenyl ether (GPE). An open reading frame (ORF) encoding a putative epoxide hydrolase (EHase) was cloned from the genome of Maritimibacter alkaliphilus KCCM 42376 [corrected], followed by expression and purification in Escherichia coli. The purified EHase (REH) hydrolyzed (S)-GPE preferentially over (R)-GPE. Enantiopure (R)-GPE from kinetic resolution of 29.2 mM racemic GPE using the purified REH could be obtained with enantiopurity of more than 99.9% enantiomeric excess (ee) and 38.4% yield (theoretical, 50%) within 20 min (enantiomeric ratio (E-value): 38.4). The enantioselective activity of REH toward GPE was also confirmed by the analysis of the vicinal diol, 3-phenoxy-1,2-propanediol. To our knowledge, this study demonstrates the highest enantioselective resolution of racemic GPE using a purified biocatalyst among the known native EHases.


Subject(s)
Alphaproteobacteria/enzymology , Epoxide Hydrolases/metabolism , Phenyl Ethers/metabolism , Amino Acid Sequence , Biocatalysis , Epoxide Hydrolases/biosynthesis , Epoxide Hydrolases/genetics , Escherichia coli/genetics , Escherichia coli/metabolism , Genome, Bacterial , Glycerol/analogs & derivatives , Glycerol/analysis , Hydrolysis , Molecular Sequence Data , Phenyl Ethers/chemistry
13.
Appl Microbiol Biotechnol ; 82(5): 873-81, 2009 Apr.
Article in English | MEDLINE | ID: mdl-19083233

ABSTRACT

A gene encoding a putative epoxide hydrolase (EHase) was identified by analyzing an open reading frame of the genome sequence of Novosphingobium aromaticivorans, retaining the conserved catalytic residues such as the catalytic triad (Asp177, Glu328, and His355) and the oxyanion hole. The enantioselective EHase gene (neh) was cloned, and the recombinant EHase could be purified to apparent homogeneity by one step of metal affinity chromatography and further characterized. The purified N. aromaticivorans enantioselective epoxide hydrolase (NEH) showed enantioselective hydrolysis toward styrene oxide, glycidyl phenyl ether, epoxybutane, and epichlorohydrin. The optimal EHase activity toward styrene oxide occurred at pH 6.5 and 45 degrees C. The purified NEH could preferentially hydrolyze (R)-styrene oxide with enantiomeric excess of more than 99% and 11.7% yield after 20-min incubation at an optimal condition. The enantioselective hydrolysis of styrene oxide was also confirmed by the analysis of the vicinal diol, 1-phenyl-1,2-ethanediol. The hydrolyzing rates of the purified NEH toward epoxide substrates were not affected by as high as 100 mM racemic styrene oxide.


Subject(s)
Epoxide Hydrolases , Sphingomonadaceae/enzymology , Amino Acid Motifs , Chromatography, Affinity , Cloning, Molecular , Consensus Sequence , DNA, Bacterial/analysis , Epoxide Hydrolases/biosynthesis , Epoxide Hydrolases/chemistry , Epoxide Hydrolases/genetics , Escherichia coli/metabolism , Hydrogen-Ion Concentration , Industrial Microbiology , Molecular Sequence Data , Phylogeny , Recombinant Fusion Proteins/biosynthesis , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/isolation & purification , Sequence Analysis, DNA , Substrate Specificity , Temperature
14.
J Microbiol Biotechnol ; 18(8): 1445-52, 2008 Aug.
Article in English | MEDLINE | ID: mdl-18756107

ABSTRACT

An open reading frame (ORF) encoding a putative epoxide hydrolase (EHase) was identified by analyzing the genome sequence of Sphingophyxis alaskensis. The EHase gene (seh) was cloned and expressed in E. coli. To facilitate purification, the gene was fused in-frame to 6x histidine at the C-terminus. The recombinant EHase (rSEH) was highly soluble and could be purified to apparent homogeneity by one step of metal affinity chromatography. The purified SEH displayed hydrolyzing activities toward various epoxides such as styrene oxide, glycidyl phenyl ether, epoxyhexane, epoxybutane, epichlorohydrin, and epifluorohydrin. The optimum activity toward styrene oxide was observed at pH 6.5 and 35 degrees . The purified SEH showed a cold-adapted property, displaying more than 40% of activity at low temperature of 10 degrees compared with the optimum activity. Despite the catalytic efficiency, the purified SEH did not hydrolyze various epoxides enantioselectively. Km and kcat of SEH toward (R)-styrene oxide were calculated as 4+/-0.3 mM and 7.42 s(-1), respectively, whereas Km and kcat of SEH toward (S)-styrene oxide were 5.25+/-0.3 mM and 10.08 s(-1), respectively.


Subject(s)
Epoxide Hydrolases/genetics , Epoxy Compounds/metabolism , Sphingomonadaceae/enzymology , Sphingomonadaceae/genetics , Amino Acid Sequence , Cloning, Molecular , Cold Temperature , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Epoxide Hydrolases/metabolism , Escherichia coli/enzymology , Escherichia coli/genetics , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Recombinant Proteins/metabolism , Sequence Alignment , Transformation, Genetic
15.
Clin Cancer Res ; 14(14): 4372-7, 2008 Jul 15.
Article in English | MEDLINE | ID: mdl-18628450

ABSTRACT

RLIP76 is a multifunctional membrane protein that transports glutathione conjugates of electrophilic compounds and other xenobiotics including chemotherapy agents out of cells. The protein is overexpressed in lung carcinomas, ovarian carcinomas, and melanomas. The protein also binds Ral and participates in mitotic spindle function, clathrin-dependent endocytosis, and triggers GTPase-activating protein activity. It is found throughout the cell, in membrane, cytosol, and the nucleus, and is known to shift between these compartments in response to stress. Loss of RLIP76 by antibody or antisense therapy is associated with increased sensitivity to radiation and chemotherapy. Conversely, liposomally delivered RLIP may treat poisoning and wounds.


Subject(s)
ATP-Binding Cassette Transporters/metabolism , GTPase-Activating Proteins/metabolism , Neoplasms/metabolism , Signal Transduction/physiology , ATP-Binding Cassette Transporters/genetics , GTPase-Activating Proteins/genetics , Humans , Oxidative Stress/physiology
16.
Anim Biotechnol ; 19(2): 89-103, 2008.
Article in English | MEDLINE | ID: mdl-18432400

ABSTRACT

In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFalpha-NFkappaB signal pathway, is associated with HC11 cell apoptosis.


Subject(s)
Apoptosis/physiology , Cell Differentiation/physiology , Mammary Glands, Animal/physiology , Neoplasm Proteins/biosynthesis , Animals , Apoptosis/drug effects , Caspase 3/metabolism , Caspase 7/metabolism , Cell Differentiation/drug effects , Cloning, Molecular , Epidermal Growth Factor/pharmacology , Epithelial Cells/cytology , Epithelial Cells/drug effects , Epithelial Cells/metabolism , Epithelial Cells/physiology , Female , Gene Expression Regulation/drug effects , Mammary Glands, Animal/cytology , Mammary Glands, Animal/drug effects , Mammary Glands, Animal/metabolism , Mice , Microscopy, Fluorescence , NF-kappa B/metabolism , Neoplasm Proteins/genetics , Pregnancy , RNA, Messenger/biosynthesis , RNA, Messenger/genetics , Reverse Transcriptase Polymerase Chain Reaction , Tumor Necrosis Factor-alpha/pharmacology
17.
Mar Biotechnol (NY) ; 10(4): 366-73, 2008.
Article in English | MEDLINE | ID: mdl-18214609

ABSTRACT

To develop an enantioselective epoxide hydrolase (EHase) from marine microorganisms, marine samples were collected from a variety of marine environments. Strains isolated by the capability of living on styrene oxide (SO) were screened for retaining enantioselective EHase activities toward SO by combining spectrophotometric, GC, and HPLC analysis. Consequently, one strain, JCS358, was selected, and the sequence analysis of 16S rRNA gene showed that the strain belonged to Erythrobacter cluster. Twelve additional Erythrobacter strains from this study or acquired from culture collections were thereby tested for displaying EHase activities, and most of tested strains showed enantioselective hydrolysis toward SO and glycidyl phenyl ether. Kinetic resolution of racemic SO using whole cell of Erythrobacter sp. JCS358 was performed. Enantiopure (S)-SO could be obtained with an enantiomeric excess (ee) higher than 99% after 15 h incubation. The determination of 1-phenyl-1,2-ethanediol configuration derived from racemic SO confirmed the enantioselective hydrolyzing activity of Erythrobacter sp. JCS358.


Subject(s)
Epoxide Hydrolases/metabolism , Epoxy Compounds/metabolism , Genetic Testing , Sphingomonadaceae/enzymology , Environment , Kinetics , Marine Biology , Molecular Sequence Data , Phylogeny , Sphingomonadaceae/classification , Sphingomonadaceae/isolation & purification
18.
Biosci Biotechnol Biochem ; 72(1): 70-81, 2008 Jan.
Article in English | MEDLINE | ID: mdl-18175929

ABSTRACT

In this study, we examined the expression and functions of serum amyloid A (SAA) isoforms during apoptosis of HC11 mammary gland epithelial cells. Expression of SAA mRNAs and apoptosis were increased in HC11 cells by serum withdrawal and gradually decreased upon the addition of serum, or epidermal growth factor (EGF). TNFalpha treatment of HC11 cells also induced expression of SAA genes, and the effect on SAA1 and SAA2 expression was suppressed by treatment with MG132, and in cells transfected with a dominant negative mutant form of IkappaBalpha. Similar results were observed in response to interleukin-1 (IL-1), IL-6 and interferon gamma (IFNgamma). Furthermore, overexpression of the SAA1 and SAA2 isoforms suppressed growth and accelerated apoptosis of HC11 cells by increasing caspase 3/7 and caspase 8 activities, but the apoptotic effect of tumor necrosis factor alpha (TNFalpha) on HC11 cells was not enhanced. We found that expression of SAA1 and SAA2, but not SAA3, was regulated by an NFkappaB-dependent pathway, and that overexpression of SAA isoforms accelerated the apoptosis of HC11 cells.


Subject(s)
Epithelial Cells/cytology , Epithelial Cells/physiology , Mammary Glands, Animal/cytology , Mammary Glands, Animal/physiology , Serum Amyloid A Protein/genetics , Animals , Apoptosis , Cell Differentiation , Cell Division , Cells, Cultured , Female , Mice , Protein Isoforms/genetics , Protein Isoforms/metabolism , RNA, Messenger/genetics , Recombinant Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Serum Amyloid A Protein/metabolism , Transfection
19.
Int J Syst Evol Microbiol ; 57(Pt 10): 2207-2211, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17911284

ABSTRACT

A novel marine bacterium, strain JCS350(T), was isolated from marine sediment samples collected from a cold-seep area. The 16S rRNA gene sequence of the isolate showed high similarity to that of Erythrobacter luteolus SW-109(T) (95.9 % sequence similarity). Lower 16S rRNA gene sequence similarities were shown to other members of the genus Erythrobacter (94.6-95.4 %) and members of the genus Porphyrobacter (94.5-95.2 %). Phylogenetic analysis with all members of the family Erythrobacteraceae and several members of the family Sphingomonadaceae revealed that the isolate formed a phyletic line with [Erythrobacter] luteolus that was distinct from other members of the family Erythrobacteraceae. The dominant fatty acids of strain JCS350(T) were 18 : 1omega7c, 16 : 1omega7c and cyclopropane 17 : 0. The major respiratory quinone was ubiquinone 10. The DNA G+C content was 54.5 mol%. The isolate did not contain bacteriochlorophyll a. Optimal growth required the presence of 2 % (w/v) NaCl with either 0.18 % CaCl(2) or 0.59 % MgCl(2), at pH 6.5 and at 35 degrees C. On the basis of the evidence of this polyphasic taxonomic study, strain JCS350(T) should be classified in a novel genus and species in the family Erythrobacteraceae, for which the name Altererythrobacter epoxidivorans gen. nov., sp. nov. is proposed. The misclassified species [Erythrobacter] luteolus is transferred to the new genus as Altererythrobacter luteolus comb. nov. The type strain of Altererythrobacter epoxidivorans is JCS350(T) (=KCCM 42314(T) =JCM 13815(T)) and the type strain of Altererythrobacter luteolus is SW-109(T) (=KCTC 12311(T) =JCM 12599(T)).


Subject(s)
Alphaproteobacteria/classification , Alphaproteobacteria/isolation & purification , Epoxide Hydrolases/metabolism , Geologic Sediments/microbiology , Alphaproteobacteria/enzymology , Alphaproteobacteria/genetics , Bacteriochlorophyll A/analysis , Base Composition , Calcium Chloride/metabolism , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Fatty Acids/analysis , Genes, rRNA , Hydrogen-Ion Concentration , Magnesium Chloride/metabolism , Molecular Sequence Data , Phylogeny , Quinones/analysis , RNA, Bacterial/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Sodium Chloride/metabolism , Temperature
20.
Appl Microbiol Biotechnol ; 77(1): 107-15, 2007 Nov.
Article in English | MEDLINE | ID: mdl-17712554

ABSTRACT

Vibrio sp. GMD509, a marine bacterium isolated from eggs of the sea hare, exhibited lipolytic activity on tributyrin (TBN) plate, and the gene representing lipolytic activity was cloned. As a result, an open reading frame (ORF) consisting of 1,017 bp (338 aa) was found, and the deduced amino acid sequence of the ORF showed low similarity (< 20%) to alpha/beta hydrolases such as dienelactone hydrolases and esterase/lipase with G-X(1)-S-X(2)-G sequence conserved. Phylogenetic analysis suggested that the protein belonged to a new family of esterase/lipase together with various hypothetical proteins. The enzyme was overexpressed in Escherichia coli and purified to homogeneity. The purified enzyme (Vlip509) showed the best hydrolyzing activity toward p-nitrophenyl butyrate (C(4)) among various p-nitrophenyl esters (C(2) to C(18)), and optimal activity of Vlip509 occurred at 30 degrees C and pH 8.5, respectively. Kinetic parameters toward p-nitrophenyl butyrate were determined as K (m) (307 muM), k (cat) (5.72 s(-1)), and k (cat)/K (m) (18.61 s(-1) mM(-1)). Furthermore, Vlip509 preferentially hydrolyzed the S-enantiomer of racemic ofloxacin ester. Despite its sequence homology to dienelactone hydrolase, Vlip509 showed no dienelactone hydrolase activity. This study represents the identification of a novel lipolytic enzyme from marine environment.


Subject(s)
Bacterial Proteins/metabolism , Carboxylic Ester Hydrolases/metabolism , Esterases/metabolism , Vibrio/enzymology , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Carboxylic Ester Hydrolases/chemistry , Carboxylic Ester Hydrolases/genetics , Cloning, Molecular , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , Electrophoresis, Polyacrylamide Gel , Esterases/chemistry , Esterases/classification , Hydrogen-Ion Concentration , Molecular Sequence Data , Phylogeny , Recombinant Proteins/chemistry , Recombinant Proteins/isolation & purification , Recombinant Proteins/metabolism , Sequence Analysis, DNA , Sequence Homology, Amino Acid , Stereoisomerism , Substrate Specificity , Temperature , Vibrio/classification , Vibrio/genetics
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