Anastomosis Groups of Rhizoctonia solani and Binucleate Rhizoctonia Associated with Potatoes in Idaho.

A survey of the relative incidence of anastomosis groups (AGs) of Rhizoctonia spp. associated with potato disease was conducted in Idaho, the leading potato producing state in the U.S.A. In total, 169 isolates of Rhizoctonia solani and seven binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants. The AG of each isolate was determined through real-time PCR assays for AG 3-PT and phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. AG 3-PT was the predominant AG, accounting for 85% of isolates recovered, followed by AG 2-1 (5.7%) and AG 4 HG-II (4.5%). Two different subsets of AG 2-1 isolates were recovered (subset 2 and 3). Three isolates each of AG A and AG K were recovered, as well as one isolate each of AG 5 and AG W. An experiment carried out under greenhouse conditions with representative isolates of the different AGs recovered from Idaho potatoes showed differences in aggressiveness between AGs to potato stems, with AG 3-PT being the most aggressive followed by an isolate of AG 2-1 (subset 3). The three BNR isolates representative of AG A, AG K, and AG W appeared to be less aggressive to potato stems than the R. solani isolates except for the AG 2-1 (subset 2) isolate. This is the first comprehensive study of the relative incidences of Rhizoctonia species associated with Idaho potatoes and the first study to report the presence of BNR AG W outside of China.

Diaporthe diversity and pathogenicity revealed from a broad survey of grapevine diseases in Europe.

Species of Diaporthe are considered important plant pathogens, saprobes, and endophytes on a wide range of plant hosts. Several species are well-known on grapevines, either as agents of pre- or post-harvest infections, including Phomopsis cane and leaf spot, cane bleaching, swelling arm and trunk cankers. In this study we explore the occurrence, diversity and pathogenicity of Diaporthe spp. associated with Vitis vinifera in major grape production areas of Europe and Israel, focusing on nurseries and vineyards. Surveys were conducted in Croatia, Czech Republic, France, Hungary, Israel, Italy, Spain and the UK. A total of 175 Diaporthe strains were isolated from asymptomatic and symptomatic shoots, branches and trunks. A multi-locus phylogeny was established based on five genomic loci (ITS, tef1, cal, his3 and tub2), and the morphological characters of the isolates were determined. Preliminary pathogenicity tests were performed on green grapevine shoots with representative isolates. The most commonly isolated species were D. eres and D. ampelina. Four new Diaporthe species described here as D. bohemiae, D. celeris, D. hispaniae and D. hungariae were found associated with affected vines. Pathogenicity tests revealed D. baccae, D. celeris, D. hispaniae and D. hungariae as pathogens of grapevines. No symptoms were caused by D. bohemiae. This study represents the first report of D. ambigua and D. baccae on grapevines in Europe. The present study improves our understanding of the species associated with several disease symptoms on V. vinifera plants, and provides useful information for effective disease management.

Variation in Fungicide Sensitivity Among Rhizoctonia Isolates Recovered from Potatoes in South Africa.

Rhizoctonia is a major pathogen of potato causing substantial yield losses worldwide. Control of Rhizoctonia diseases is based predominantly on the application of fungicides. However, little is known about the fungicide response variability of different Rhizoctonia anastomosis groups associated with potato diseases in South Africa. A total of 131 Rhizoctonia isolates were obtained from potato growing regions of South Africa from 2012 to 2014 and evaluated for sensitivity to fungicides in vitro and in vivo. The fungicides comprised six chemical formulations and one bio-fungicide representing seven Fungicide Resistance Action Committee groups. All Rhizoctonia anastomosis groups were sensitive to tolclofos-methyl (EC50: 0.001 to 0.098 µg a.i. ml-1) and fludioxonil (EC50: 0.06 to 0.09 µg a.i. ml-1) and showed variation in sensitivity to pencycuron, iprodione, benomyl, and Bacillus subtilis QST 713. However, for azoxystrobin, Rhizoctonia isolates exhibited variable sensitivity ranging from sensitivity (EC50: <0.09 µg a.i. ml-1) to insensitivity with EC50 values exceeding 5 µg a.i. ml-1. In greenhouse and field trials, tolclofos-methyl and fludioxonil exhibited significantly greater control of stem and black scurf whereas azoxystrobin was the least effective. This work demonstrated variable sensitivity within and among anastomosis groups of R. solani and binucleate Rhizoctonia to different fungicides. Information on fungicide sensitivity of Rhizoctonia isolates is crucial in the development of effective Rhizoctonia control strategies and facilitates monitoring of fungicide insensitive isolates in the pathogen population.

Fourier transform infra-red spectroscopy using an attenuated total reflection probe to distinguish between Japanese larch, pine and citrus plants in healthy and diseased states.

FTIR spectroscopy coupled with an Attenuated Total Reflection (ATR) sampling probe has been demonstrated as a technique for detecting disease in plants. Spectral differences were detected in Japanese Larch (Larix kaempferi) infected with Phytophthora ramorum at 3403cm(-1) and 1730cm(-1), from pine (Pinus spp.) infected with Bursaphelenchus xylophilus at 1070cm(-1), 1425cm(-)1, 1621cm(-1) and 3403cm(-1) and from citrus (Citrus spp.) infected with 'Candidatus liberibacter' at 960cm(-1), 1087cm(-1), 1109cm(-1), 1154cm(-1), 1225cm(-1), 1385cm(-1), 1462cm(-1), 1707cm(-1), 2882cm(-1), 2982cm(-1) and 3650cm(-1). A spectral marker in healthy citrus has been identified as Pentanone but is absent from the diseased sample spectra. This agrees with recent work by Aksenov, 2014. Additionally, the spectral signature of Cutin was identified in the spectra of Pinus spp. and Citrus spp. and is consistent with work by Dubis, 1999 and Heredia-Guerrero, 2014.

Anastomosis Groups and Pathogenicity of Rhizoctonia solani and Binucleate Rhizoctonia from Potato in South Africa.

A survey of anastomosis groups (AG) of Rhizoctonia spp. associated with potato diseases was conducted in South Africa. In total, 112 Rhizoctonia solani and 19 binucleate Rhizoctonia (BNR) isolates were recovered from diseased potato plants, characterized for AG and pathogenicity. The AG identity of the isolates was confirmed using phylogenetic analysis of the internal transcribed spacer region of ribosomal DNA. R. solani isolates recovered belonged to AG 3-PT, AG 2-2IIIB, AG 4HG-I, AG 4HG-III, and AG 5, while BNR isolates belonged to AG A and AG R, with frequencies of 74, 6.1, 2.3, 2.3, 0.8, 12.2, and 2.3%, respectively. R. solani AG 3-PT was the most predominant AG and occurred in all the potato-growing regions sampled, whereas the other AG occurred in distinct locations. Different AG grouped into distinct clades, with high maximum parsimony and maximum-likelihood bootstrap support for both R. solani and BNR. An experiment under greenhouse conditions with representative isolates from different AG showed differences in aggressiveness between and within AG. Isolates of AG 2-2IIIB, AG 4HG-III, and AG R were the most aggressive in causing stem canker while AG 3-PT, AG 5, and AG R caused black scurf. This is the first comprehensive survey of R. solani and BNR on potato in South Africa using a molecular-based approach. This is the first report of R. solani AG 2-2IIIB and AG 4 HG-I causing stem and stolon canker and BNR AG A and AG R causing stem canker and black scurf on potato in South Africa.

First Report of Rhizoctonia solani AG 4HG-III Causing Potato Stem Canker in South Africa.

Black scurf and stem canker caused by Rhizoctonia solani Kühn (teleomorph: Thanathephorus cucumeris Frank Donk) are potato diseases of worldwide economic importance (4). R. solani consists of 13 anastomosis groups (AGs) of which AG 3-PT is considered the dominant causal agent of potato diseases globally (1,4). However, other AGs such as AG 2-1, 5, and 8 have been reported to cause potato diseases (1,4). In February 2013, potato stem samples (cv. Mondial) displaying dark brown lesions resembling those caused by Rhizoctonia stem canker were obtained from a commercial field in Limpopo Province, South Africa. Symptomatic tissue was disinfected with 1% NaOCl for 1 min, rinsed in sterile water, and 4-mm stem pieces excised from the margins of symptomatic tissues and plated on 2% water agar supplemented with 20 mg/l of chloramphenicol. Single hyphal tips taken from fungal isolates identified as R. solani based on morphological traits (3) were transferred to potato dextrose agar. DNA was isolated from the resulting cultures and ITS region of rDNA was sequenced as previously described (2). The resulting sequences of three of the isolates, Rh 81, Rh 82, and Rh 83 (KF712285, KF712286, and KF712287), were 99% similar to those of AG 4 HG-III found in GenBank (DQ102449 and AF354077). Therefore, based on molecular methods, these three isolates were identified as R. solani AG4 HG-III. To determine pathogenicity of the AG4 HG-III isolates, certified disease free mini-tubers (Generation 0, cv. Mondial, produced in tunnels) were used in pot trials. PDA plugs of each isolate were added to 10 g of barley grains, which had been sterilized by autoclaving for two consecutive days at 121°C for 30 min, and were incubated for 14 days until fully colonized. Ten colonized barley grains were placed 10 mm above each mini-tuber planted in 5l pots containing sterile potting mixture of sand:clay:pinebark (1:1:1). Ten tubers were inoculated with each isolate. Uninoculated, sterile barley grains were applied to the control treatment. Mini-tubers were grown in a greenhouse maintained at 22°C with light for a 12 h day. After 7 weeks, five plants for each isolate were destructively sampled and assessed for stem canker symptoms. At 120 days after sowing, the remaining five plants per treatment were assessed for blemishes on progeny tubers. The stem canker incidences of plants inoculated with Rh 81, Rh 82, and Rh 83 were 25, 25, and 50%, respectively, whereas no symptoms were observed in control plants. Sclerotia formation and blemishes were not observed on any of the progeny tubers, which might indicate that these strains are only able to infect stems, or that environmental conditions were not suitable for tuber blemish or black scurf development. R. solani AG4 HG-III was consistently re-isolated from symptomatic stems displaying brown lesions, and the identity of the re-isolates were confirmed by molecular tests as previously described, thereby fulfilling Koch's postulates. To our knowledge, this is the first report of R. solani AG4 HG-III causing stem canker on potato in South Africa and worldwide. Knowledge of which AGs are present in crop production systems is important when considering disease management strategies such as crop rotation and fungicide treatments (3). References: (1) C. Campion et al. Eur. J. Plant. Pathol. 109:983, 2003. (2) N. Muzhinji et al. Plant Dis. 98:570, 2014. (3) L. Tsror. J. Phytopathol. 158:649, 2010. (4) J. W. Woodhall et al. Plant. Pathol. 56:286, 2007.

Elephant Hide and Growth Cracking on Potato Tubers Caused by Rhizoctonia solani AG3-PT in South Africa.

Rhizoctonia solani consists of 13 anastomosis groups (AGs) designated AG1 to 13. AG3-PT is considered the predominant AG in potatoes (4) and is associated with quantitative and qualitative yield losses. Qualitative losses are typically associated with the tuber blemish disease, black scurf. However, atypical tuber blemishes such as elephant hide consisting of corky lesions on the tuber surface (2) have also been attributed to Rhizoctonia. Such atypical blemishes are not considered specific to Rhizoctonia, making direct-cause effect estimates difficult (1). Koch's postulates for the elephant hide symptom and R. solani AG3-PT have not been completed. Recently, growth cracking and scab lesions were observed on potato tubers in South Africa and attributed to a new Streptomyces species (3). These lesions and cracks were similar to elephant hide symptoms attributed to R. solani AG3-PT. Therefore, the cause of the elephant hide symptom in South Africa was investigated further. Symptoms of elephant hide and cracking have been observed on tubers from the Eastern Free State, KwaZulu-Natal, Limpopo, Mpumalanga, North-Eastern Cape, Northern Cape, North West, Sandveld, and Western Free State growing regions. In 2012, three samples of potato tubers (cv. BP1) with elephant hide and cracking were selected for analysis. These samples were collected from Clanwilliam in the Sandveld potato growing region. Tubers were surface sterilized with 1% NaOCl; sections of affected tissue were excised and plated onto potato dextrose agar (PDA). Rhizoctonia-like colonies were identified and after further sub-culturing on PDA, three representative isolates (Rh3, Rh4, and Rh6) of R. solani from each sample were obtained. For each isolate, genomic DNA was extracted and the rDNA ITS region sequenced using ITS1-F and ITS4 (2). The resulting sequences (KF234142, KF234143, and KF234144) were at least 98% identical to other AG3-PT sequences on GenBank (JX27814 and KC157664). To confirm Koch's postulates, pathogenicity tests were conducted with the three isolates. PDA plugs of each isolate were added to 10 g of barley grains which were incubated for 14 days until fully colonized. The barley grains were then used to inoculate disease-free mini-tubers (cv. BP1) in 5l pots containing a sand-clay-pine bark mixture (1:1:1 ratio). Potato plants inoculated with sterile barley grains served as controls. Plants were held for 120 days in a greenhouse at 22°C with light for 12 h a day. Incidence of the elephant hide symptom for isolates Rh3, Rh4, and Rh6 was 58%, 33%, and 37.5%, respectively. Growth cracking and black scurf were also observed with each isolate. R. solani AG3-PT was successfully re-isolated from symptomatic tubers, confirming Koch's postulates. This is the first report of R. solani AG3-PT causing elephant hide in potato tubers in South Africa. Elephant hide caused by R. solani AG3-PT has been reported in tubers from France (2) and the United Kingdom (3), but Koch's postulates were not proven. In this study, Koch's postulates were proven for R. solani AG3-PT causing scab or elephant hide symptom and cracking in potato tubers. R. solani AG3-PT should thus be considered in addition to Streptomyces as a cause of this symptom and control strategies should also consider R. solani AG3-PT. References: (1) G. J. Banville et al. Pages 321-330 in: Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Disease Control, B. Sneh et al., eds. Kluwer Academic Publishers, Dordrecht, The Netherlands, 1996. (2) M. Fiers et al. Eur. J. Plant. Pathol. 128:353, 2010. (3) R. Gouws and A. McLeod. Plant Dis. 96:1223, 2012. (4) J. W. Woodhall et al. Eur. J. Plant. Pathol. 136:273, 2013.

Rhizoctonia solani Anastomosis Group 3 is Predominant in Potato Tubers in Poland.

Rhizoctonia solani is a species complex of 13 related but genetically distinct anastomosis groups (AGs). In potato, R. solani can infect the stems, stolons, and roots, resulting in quantitative losses. It can also cause qualitative losses through blemishes occurring on progeny tubers, such as black scurf and elephant hide (corky cracking). Knowledge of the AG in local populations is important because they differ in host range, fungicide sensitivity, and disease severity (2). To determine the AGs present in Poland, 54 tuber samples displaying typical R. solani symptoms were taken from six different fields in 2011. The fields were representative of five different administrative regions of Poland and from at least 10 different varieties. Rhizoctonia was isolated from tubers by placing symptomatic material on to tap water agar amended with streptomycin and penicillin and after 2 to 3 days Rhizoctonia colonies were identified and hyphal tips of these transferred to potato dextrose agar. Rhizoctonia was successfully isolated from 48 tubers displaying black scurf and two tubers displaying elephant hide symptoms. DNA was extracted from Rhizoctonia cultures using a Wizard Food kit (Promega) and the AG was determined using specific real-time PCR assays (1). All Rhizoctonia isolates were determined to be AG3 and this was confirmed for 10 selected isolates by observing hyphal fusion with a known AG3 tester isolate (Rs08) as described previously (3). Pairings were also conducted amongst the 10 Polish isolates, C2 reactions were typically observed indicating numerous vegetative compatible groups are present. This study shows that AG3 is likely to be the predominant AG in potato tubers in Poland. This is similar to other studies in Europe, which have all determined that AG3 accounts for at least 92% of isolates from potato (2,3). AG2-1, 4, and 5 have also been found in tubers worldwide and climate and certain crop rotations can influence the presence of these other AGs in potato tubers (2). However, climate and crop rotations in Poland are similar to other parts of Europe so the predominance of AG3 is expected. AG3 was also isolated from elephant hide symptoms; however, it was more frequently isolated from sclerotia. The ability of AG3 to prolifically produce sclerotia and thereby survive on seed tubers may explain its predominance in potato crops (4). Therefore, studies focusing on the management of Rhizoctonia potato disease in Poland should consider AG3 in the first instance. References: (1) G. E. Budge et al. Plant Pathol. 58:1071, 2009. (2) L. Tsror. J. Phytopathol. 158:649, 2010. (3) J. W. Woodhall et al. Plant Pathol. 56:286, 2007. (4) J. W. Woodhall et al. Plant Pathol. 57:5, 2008.

First Report of a Binucleate Rhizoctonia (AG-A) from Potato Stems Infecting Potatoes and Sugarbeet in the Pacific Northwest.

Rhizoctonia solani causes economically important diseases on potatoes and sugarbeet throughout the world (2). R. solani is a species complex of 13 anastomosis groups (AGs) of which R. solani AG3-PT is most commonly associated with potato and AG2-2 and AG4 with sugarbeet. However, several AGs, including AG2-2 and AG4, have been recorded causing potato diseases (2,3). In summer 2012, plants in potato fields in Idaho were sampled for R. solani. Isolations were attempted from symptomatic plants. DNA extracted from the resulting pure Rhizoctonia cultures was screened using a real-time PCR assay for AG3-PT (3). For the isolates that tested negative for AG3-PT, AG was determined by amplifying and sequencing the rDNA internal transcribed spacer (ITS) region using the primers ITS5 (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS4 (5'-TCCTCCGCTTATTGATATGC-3'). The resulting sequences of two isolates (isolates 204 and 206, GenBank Accession No. KC782951) shared 99% identity with other AG-A isolates (AY927358 and AY927356). Koch's postulates were confirmed for isolate 206 by placing five 10-mm plugs, from 10-day-old potato dextrose agar (PDA) cultures, onto the surface of a soil-less potting mix (composed of peat moss, perlite, and sand) of 1-liter pots, where non-inoculated PDA plugs served as a control. Each pot contained a 'Rosara' seed tuber or three ungerminated (BETASEED - BTS 27RR10) sugarbeet seeds (n = 5). Pots were incubated in a glasshouse between 18 and 22°C for 1 month and then assessed for disease. For potatoes, a pigmented necrosis was observed at the soil interface in 88% of the stems and plants were stunted relative to the non-inoculated controls. A significant reduction in root growth was observed in 60% of the germinated sugarbeet plants. Control plants of both potatoes and beets were asymptomatic. For reisolation, 1-cm sections were taken from each potato stem and germinated beet plant, surface sterilized, and placed on alkaline water agar. The reisolated fungi were identified using morphology and a subset was confirmed by sequencing. Isolate 206 was successfully recovered from 84% of the potato stems and from 20% of the sugarbeet seedlings. In a similar experiment, 2-month-old potato and sugarbeet plants were inoculated using 50 g of autoclaved barley grains (inoculated with isolate 206) per 1-liter pot. Between 40 and 60% of inoculated plants appeared stunted in both cases. Pigmented necrosis was observed at the soil interface on 45% of the potato stems and reduced root growth was observed in the 50% of the sugarbeet plants. Control plants were asymptomatic. To our knowledge, this is the first report of the binucleate AG-A causing disease in Idaho on potato stems. BNR species have previously been isolated from potato (4) and sugarbeet plants (1). The binucleate Rhizoctonia AG-A caused disease on potato stems and sugarbeet roots and was readily reisolated. Since sugarbeet is commonly grown in rotation with potato in Idaho, such a rotation could increase the risk of soilborne infection to either crop by AG-A. It is known that AGs can differ in fungicide sensitivity (2), and thus a knowledge of which AGs may be present is important when considering disease management strategies. References: (1) C. A. Strausbaugh et al. Can. J. Plant Pathol. 33:210, 2011. (2) L. Tsror. Biology, Epidemiology and Management of Rhizoctonia solani on Potato 158:649, 2010. (3) J. Woodhall et al. Eur. J. Plant Pathol. 136:273, 2013. (4) Y. G. Yang and X. H. Wu. Plant Dis. 97:1246, 2013.

First Report of Rhizoctonia solani AG2-2IIIB Infecting Potato Stems and Stolons in the United States.

Rhizoctonia solani is an important pathogen of potato (Solanum tuberosum) causing qualitative and quantitative losses. It has been associated with black scurf and stem canker. Isolates of the fungus are assigned to one of 13 known anastomosis groups (AGs), of which AG3 is most commonly associated with potato disease (2,4). In August 2011, diseased potato plants originating from Rupert, ID (cv. Western Russet) and Three Rivers, MI (cv. Russet Norkotah) were received for diagnosis. Both samples displayed stem and stolon lesions typically associated with Rhizoctonia stem canker. The presence of R. solani was confirmed through isolation as previously described (4) and the Idaho and Michigan isolates were designated J11 and J8, respectively. AG was determined by sequencing the rDNA internal transcribed spacer (ITS) region using primers ITS5 and ITS4 (3). The resulting sequences of the rDNA ITS region of isolates J8 and J11 (GenBank Accession Nos. HE608839 and HE608840, respectively) were between 97 and 100% identical to that of other AG2-2IIIB isolates present in sequence databases (GenBank Accession Nos. FJ492075 and FJ492170, respectively). Koch's postulates were confirmed for each isolate by carrying out the following protocol. Each isolate was cultured on potato dextrose agar for 14 days. Five 10-mm agar plugs were then placed on top of seed tubers (cv. Maris Piper) in 1-liter pots containing John Innes Number 3 compost (John Innes Manufacturers Association, Reading, UK). Pots were held in a controlled environment room at 18°C with 50% relative humidity and watered as required. After 21 days, plants were removed and assessed for disease. Typical Rhizoctonia stem lesions were observed and R. solani was successfully reisolated from symptomatic material. To our knowledge, this is the first report of AG2-2IIIB causing disease on potatoes in the United States. In the United States, AGs 2-1, 3, 4, 5, and 9 have all been previously implicated in Rhizoctonia potato disease (2). AG2-2IIIB should now also be considered a potato pathogen in the United States. Knowledge of which AG is present is invaluable when considering a disease management strategy. AG2-2IIIB is a causal agent of sugar beet (Beta vulgaris) root rot in Idaho (1). Sugar beet is commonly grown in crop rotation with potato and such a rotation could increase the risk of soilborne infection to either crop by AG2-2IIIB. References: (1) C. A. Strausbaugh et al. Can. J. Plant Pathol. 33:210, 2011. (2) L. Tsror. J. Phytopatol. 158:649, 2010. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, Inc., New York, 1990. (4) J. W.Woodhall et al. Plant Pathol. 56:286, 2007.

First Report of Rhizoctonia solani AG4 HG-II Infecting Potato Stems in Idaho.

The fungus Rhizoctonia solani is the causal agent of stem canker and black scurf of potato (Solanum tuberosum). R. solani is a species complex consisting of 13 anastomosis groups (AGs) designated AG1 to 13 (2, 3). Stems of potato (cv. Russet Norkotah) with brown lesions were recovered from one field in Kimberley, Idaho, in August 2011. Using previously described methods (3), R. solani was recovered from the symptomatic stems and one representative isolate (J15) was selected for further characterization. Sequencing of the rDNA ITS region of isolate J15 was undertaken as previously described (3) and the resulting rDNA ITS sequence (HE667745) was 99% identical to sequences of other AG4 HG-II isolates in GenBank (AF354072 and AF354074). Pathogenicity of the isolate was determined by conducting the following experiment. Mini-tubers of cv. Santé were planted individually in 1-liter pots containing John Innes Number 3 compost (John Innes Manufacturers Association, Reading, UK). Pots were either inoculated with J15, an isolate of AG3-PT (Rs08), or were not inoculated. Each treatment was replicated four times. Inoculum consisted of five 10-mm-diameter potato dextrose agar plugs, fully colonized by the appropriate isolate, placed in the compost approximately 40 mm above each seed tuber. Pots were held in a controlled environment room at 21°C with 50% relative humidity and watered as required. After 21 days, plants were assessed for disease. No symptoms of the disease were present in non-inoculated plants. In the Rs08 (AG3-PT) inoculated plants, all stems displayed large brown lesions and 20% of the stems had been killed. No stem death was observed in J15 (AG4 HG-II) inoculated plants. However, brown lesions were observed in three of the four J15 (AG4 HG-II) inoculated plants. These lesions were less severe than in plants inoculated with the Rs08(AG3-PT) inoculated plants and were present in 40% of the main stems. In the J15 (AG4 HG-II) inoculated pots, R. solani AG4 HG-II was reisolated from the five symptomatic stems, thereby satisfying Koch's postulates. To our knowledge, this is the first report of AG4 HG-II causing disease on potatoes in Idaho. AG4 has been isolated from potato previously from North Dakota, although the subgroup was not identified (1). The only previous report where AG4 HG-II was specifically determined to cause disease on potato was in Finland, but the isolate could not be maintained and Koch's postulates were not completed (3). The present study shows that AG4 HG-II can cause stem disease in potatoes, although disease does not develop as severely or as consistently as for AG3-PT. However, as demonstrated with isolates of AG2-1 and AG5, even mild stem infection can reduce tuber yield by as much as 12% (4). AG4 HG-II is a pathogen of sugar beet in Idaho, which was grown previously in this field. This history may have contributed to high levels of soilborne inoculum required to produce disease on potato. References: (1) N. C. Gudmestad et al. Page 247 in: J. Vos et al. eds. Effects of Crop Rotation on Potato Production in the Temperate Zones. Kluwer, Dordrecht, Netherlands, 1989. (2) M. J. Lehtonen et al. Agric. Food Sci. 18:223, 2009. (3) J. W. Woodhall et al. Plant Pathol. 56:286, 2007. (4) J. W. Woodhall et al. Plant Pathol. 57:897, 2008.