Subject(s)
Glycolysis/drug effects , Insulin Antagonists , Insulin/pharmacology , Mammary Neoplasms, Experimental/metabolism , Testolactone/pharmacology , Testosterone/pharmacology , Animals , Female , Fructose/metabolism , Glucose/metabolism , In Vitro Techniques , Mice , Mice, Inbred C3H , Time FactorsSubject(s)
Carcinoma, Hepatocellular/metabolism , Glycolysis , Liver Neoplasms/metabolism , Adenocarcinoma/metabolism , Adenoma, Bile Duct/metabolism , Anaerobiosis , Animals , Bile Duct Neoplasms/metabolism , Female , Genotype , Glucosamine/pharmacology , Glucose/metabolism , Glucose/pharmacology , In Vitro Techniques , Liver/metabolism , Mammary Neoplasms, Experimental/metabolism , Manometry , Mice , Mice, Inbred C3H , Neoplasm Transplantation , Neoplasms, Experimental/metabolism , Neoplasms, Multiple Primary , Organ Size , Testolactone/pharmacology , Transplantation, HomologousABSTRACT
Pulsed nuclear magnetic resonance has been used to differentiate in vivo between normal mouse tail tissue and a malignant transplanted melanoma, S91, located on the tail. The tumor displayed a nuclear (proton) spin-lattice relaxation time of approximately 0.7 second contrasted with the simultaneously measured normal tail tissue relaxation time of approximately 0.3 second.
Subject(s)
Magnetic Resonance Spectroscopy , Neoplasms/diagnosis , Animals , Melanoma/diagnosis , Mice , Neoplasms, Experimental/diagnosis , TailSubject(s)
Cell Transformation, Neoplastic , Neoplasms, Experimental , Acid Phosphatase/analysis , Alkaline Phosphatase/analysis , Animals , Arginase/analysis , Blood , Cell Transformation, Neoplastic/drug effects , Chromosomes , Culture Media , Culture Techniques , Embryo, Mammalian , Germ-Free Life , Glycolysis , Mice , Microscopy, Electron , Neoplasms, Experimental/enzymology , Neoplasms, Experimental/genetics , Neoplasms, Experimental/metabolism , Neoplasms, Experimental/pathology , Ribosomes , Time FactorsSubject(s)
Carcinoma, Ehrlich Tumor/metabolism , Carcinoma, Krebs 2/metabolism , Fluorouracil/pharmacology , Glycolysis/drug effects , Pyrimidines/pharmacology , Adenine/pharmacology , Animals , Arsenic/pharmacology , Deoxyuridine/pharmacology , Fructose/antagonists & inhibitors , Fructose/pharmacology , Glucose/antagonists & inhibitors , Glucose/metabolism , Glutamine/pharmacology , In Vitro Techniques , Manometry , Mice , Nucleosides/pharmacology , Phenols/pharmacology , Phosphates/metabolism , Pyruvates/pharmacology , Respiration , Succinates/pharmacology , Uracil/pharmacology , Uridine/pharmacologyABSTRACT
When four thiazine dyes were added to ascites cell suspensions to yield 10(-4) M final concentration, the order of increasing effectiveness, for both stimulation of aerobic glycolysis in the dark and inhibition of aerobic glycolysis in the presence of added light, was thionine, methylene blue, thionine blue, and new methylene blue. After illumination, inhibition of anaerobic glycolysis occurred in the same respective dye order. Studies in depth of photodynamic effects of methylene blue on Ehrlich ascites cells revealed marked metabolic effects of light and 10(-4) M dye or lower. Diphosphopyridine nucleotide (DPN) with 5 X 10(-6) M methylene blue and light almost completely abolished the Pasteur effect after only 10 minutes' illumination of the dye-cell-coenzyme system. The ability of light, methylene blue, and exogenous DPN to specifically inhibit the Pasteur effect is consistent with the view that DPN can be important to proper functioning of the Pasteur effect mechanism. It is suggested that perfusion with some of the less toxic photosensitive dyes in combination with conventional or lasered light may result in significant selective destruction of tumor cells in vivo.
Subject(s)
Carcinoma, Ehrlich Tumor/drug therapy , Glycolysis/drug effects , Methylene Blue/pharmacology , NAD/pharmacology , Photochemotherapy , Animals , Carcinoma, Ehrlich Tumor/metabolism , Cell Respiration/drug effects , Enzyme Inhibitors/pharmacology , Methylene Blue/chemistry , Phenothiazines/pharmacology , Time Factors , Tumor Cells, Cultured , Zinc Compounds/pharmacologyABSTRACT
Certain responses of mouse and hamster cells to polyoma virus were examined with respect to their specificity as "indicators" of neoplastic conversion in vitro. These responses included the development of transplantation antigens and changes in morphologic growth pattern, cytology, karyology, rates of proliferation, and glycolytic activities. Under limited conditions, i.e., in short-term, slow-growing cultures, the morphologic change in growth pattern and increases in glycolytic activity and proliferation rate induced by polyoma virus appeared to correlate with neoplastic conversion. However, in long-term or rapidly growing short-term cultures, similar morphologic patterns occurred in cells that subsequently tested as non-neoplastic. Also, such patterns could be induced by polyoma virus in cells already neoplastic. Cells that had undergone "spontaneous" neoplastic conversion frequently showed none of these morphologic features of virus-transformed cells. Prolonged culture of cells without added virus resulted in increased glycolytic activities and proliferation rates equivalent to those of virus-transformed cells. These changes occurred in at least one cell line long before evidence of neoplastic conversion. The cytologic changes in the virus-treated neoplastic cells were similar to those usually associated with neoplastic cells in vivo and may possibly serve as sensitive indicators of in vitro neoplastic conversion. From the observations of this study, the change in morphologic growth pattern is interpreted not as loss of "contact inhibition," but as a proliferative response accompanied by decreased adhesion of cells to the glass substrate.
