Subject(s)
Blood Proteins/metabolism , Polyphosphates/metabolism , Technetium Tc 99m Medronate/analogs & derivatives , Technetium Tc 99m Medronate/metabolism , Technetium Tc 99m Pyrophosphate , Technetium/metabolism , Tin Polyphosphates/metabolism , Chromatography, Gel , Erythrocytes/metabolism , Humans , In Vitro Techniques , Protein BindingABSTRACT
The preparation of 99mTc(Sn)HMDP was investigated as a function of pH, Sn(II) and ligand concentration. HMDP could be labeled efficiently from pH 2-9. The Sn(II) and the ligand concentrations had a beneficial influence. The composition of the radiopharmaceutical under various experimental conditions was studied by means of gel chromatography on Biogel P-4. Six different complexes were found. A preparation consisted of maximally three major complexes. The presence of a particular complex was mainly determined by pH and ligand concentration. The Sn(II) concentration had little influence.
Subject(s)
Bone and Bones/diagnostic imaging , Technetium Tc 99m Medronate/analogs & derivatives , Chromatography, Gel , Humans , Hydrogen-Ion Concentration , Radionuclide Imaging , Technetium Tc 99m Medronate/chemical synthesis , Technetium Tc 99m Medronate/isolation & purificationABSTRACT
Diacylphosphatidylethanolamine (PE) was isolated from mouse and rat heart given the same standard diet. The molecular species of PE were determined after conversion of PE into diglycerides by means of hydrolysis with phospholipase C, subsequent hydrolysis with pancreatic lipase and separation of the products by argentation TLC and capillary gaschromatography. Docosahexaenoic acid (22:6n3) containing molecular species and arachidonic acid (20:4n6) containing molecular species represented the major fractions. A preference for stearic acid to combine with poly-unsaturated fatty acids was found. Despite an abundant presence of linoleic acid (18:2n6) in the diet, molecular species containing this fatty acid represented only a minor fraction. The possible physico-chemical and physiological meaning of the presence of molecular species containing many double bonds is discussed.
