A Comprehensive Analysis of Transcriptomics and Metabolomics Revealed Key Pathways Involved in Saccharum spontaneum Defense against Sporisorium scitamineum.

Sugarcane smut, caused by Sporisorium scitamineum, poses a severe threat to sugarcane production. The genetic basis of sugarcane resistance to S. scitamineum remains elusive. A comparative transcriptomic and metabolomic study was conducted on two wild Saccharum species of S. spontaneum with contrast smut resistance. Following infection, the resistant line exhibited greater down-regulation of genes and metabolites compared to the susceptible line, indicating distinct biological processes. Lignan and lignin biosynthesis and SA signal transduction were activated in the resistant line, while flavonoid biosynthesis and auxin signal transduction were enhanced in the susceptible line. TGA2.2 and ARF14 were identified as playing positive and negative roles, respectively, in plant defense. Exogenous auxin application significantly increased the susceptibility of S. spontaneum to S. scitaminum. This study established the significant switching of defense signaling pathways in contrast-resistant S. spontaneum following S. scitamineum infection, offering a hypothetical model and candidate genes for further research into sugarcane smut disease.

Haplotype variations of sucrose phosphate synthase B gene among sugarcane accessions with different sucrose content.

BACKGROUND: Sucrose phosphate synthase B (SPSB) gene encoding an important rate-limiting enzyme for sucrose synthesis in sugarcane is mainly expressed on leaves, where its alleles control sucrose synthesis. In this study, genetic variation of SPSB gene represented by different haplotypes in sugarcane was investigated in hybrid clones with high and low sugar content and various accessory species. RESULTS: A total of 39 haplotypes of SPSB gene with 2, 824 bp in size were identified from 18 sugarcane accessions. These haplotypes mainly distributed on Chr3B, Chr3C, and Chr3D according to the AP85-441 reference genome. Single nucleotide polymorphisms (SNPs) and insertion/deletion (InDels) were very dense (42 bp/sequence variation) including 44 transitional and 23 transversional SNPs among the 39 haplotypes. The sequence diversity related Hd, Eta, and Pi values were all lower in clones of high sucrose content (HS) than those in clones of low sucrose content (LS). The evolutionary network analysis showed that about half SPSB haplotypes (19 out of 39) were clustered into one group, including 6 (HAP4, HAP6, HAP7, HAP9, HAP17 and HAP20) haplotypes under positive selection in comparison to HAP26 identified in Badila (S. officinarum), an ancestry noble cane species and under purification selection (except HAP19 under neutral selection) in comparison to HAP18 identified from India1 (S. spontaneum), an ancestry species with low sugar content but high stress tolerance. The average number of haplotypes under positive selection in HS clones was twice as that in LS. Most of the SNPs and InDels sequence variation sites were positively correlated with sucrose and fiber content and negatively correlated with reducing sugar. CONCLUSIONS: A total of 39 haplotypes of SPSB gene were identified in this study. Haplotypes potentially associated with high sucrose synthesis efficiency were identified. The mutations of SPSB haplotypes in HS were favorable and tended to be selected and fixed. The results of this study are informative and beneficial to the molecular assisted breeding of sucrose synthesis in sugarcane in the future.

Polyaniline as a Nitrogen Source and Lignosulfonate as a Sulphur Source for the Preparation of the Porous Carbon Adsorption of Dyes and Heavy Metal Ions.

Using agricultural and forestry wastes as raw materials, adsorbent materials were prepared for dye adsorption in wastewater, which can minimize the environmental load and fully realize sustainability by treating waste with waste. Taking lignosulfonate as a raw material, due to its molecular structure having more reactive groups, it is easy to form composite materials via a chemical oxidation reaction with an aniline monomer. After that, using a sodium lignosulfonate/polyaniline composite as the precursor, the activated high-temperature pyrolysis process is used to prepare porous carbon materials with controllable morphology, structure, oxygen, sulfur, and nitrogen content, which opens up a new way for the preparation of functional carbon materials. When the prepared O-N-S co-doped activated carbon materials (SNC) were used as adsorbents, the adsorption study of cationic dye methylene blue was carried out, and the removal rate of SNC could reach up to 99.53% in a methylene blue solution with an initial concentration of 100 mg/L, which was much higher than that of undoped lignocellulosic carbon materials, and the kinetic model conformed to the pseudo-second-order kinetic model. The adsorption equilibrium amount of NC (lignosulfonate-free) and SNC reached 478.30 mg/g and 509.00 mg/g, respectively, at an initial concentration of 500 mg/L, which was consistent with the Langmuir adsorption isothermal model, and the adsorption of methylene blue on the surface of the carbon material was a monomolecular layer. The adsorption of methylene blue dye on the carbon-based adsorbent was confirmed to be a spontaneous and feasible adsorption process by thermodynamic parameters. Finally, the adsorption of SNC on methylene blue, rhodamine B, Congo red, and methyl orange dyes were compared, and it was found that the material adsorbed cationic dyes better. Furthermore, we also studied the adsorption of SNC on different kinds of heavy metal ions and found that its adsorption selectivity is better for Cr3+ and Pb2+ ions.

Comprehensive Evaluation of Polyaniline-Doped Lignosulfonate in Adsorbing Dye and Heavy Metal Ions.

Lignosulfonate/polyaniline (LS/PANI) nanocomposite adsorbent materials were prepared by the chemical polymerization of lignosulfonate with an aniline monomer as a dopant and structure-directing agent, and the adsorption behavior of dyes as well as heavy metal ions was investigated. LS/PANI composites were used as dye adsorbents for the removal of different cationic dyes (malachite green, methylene blue, and crystal violet). The adsorption behavior of LS/PANI composites as dye adsorbents for malachite green was investigated by examining the effects of the adsorbent dosage, solution pH, initial concentration of dye, adsorption time, and temperature on the adsorption properties of this dye. The following conclusions were obtained. The optimum adsorption conditions for the removal of malachite green dye when LS/PANI composites were used as malachite green dye adsorbents were as follows: an adsorbent dosage of 20 mg, an initial concentration of the dye of 250 mg/L, an adsorption time of 300 min, and a temperature of 358 K. The LS/PANI composite adsorbed malachite green dye in accordance with the Langmuir adsorption model and pseudo-second-order kinetic model, which belongs to chemisorption-based monomolecular adsorption, and the equilibrium adsorption amount was 245.75 mg/g. In particular, the adsorption of heavy metal ion Pb2+ was investigated, and the removal performance was also favorable for Pb2+.

Bio-Based Carbon Materials for High-Performance Supercapacitors.

Lignin, one of the components of natural plant biomass, is a rich source of carbon and has excellent potential as a valuable, sustainable source of carbon material. Low-cost lignosulfonate (LS) doped with polyaniline (PANI) has been used as a precursor to produce porous carbon. LS has a highly dispersed and sparse microstructure and can be accidentally doped with S atoms. N and S double-doped carbon can be directly synthesized with abundant mesopores and high surface area in a lamellar network using PANI as another doping source. This study explored the optimal conditions of LS/PANI material with different amounts of lignosulfonate and different carbonization temperatures. When the amount of lignosulfonate was 4 g and the carbonization temperature was 700 °C, graded porous carbon was obtained, and the electrochemical performance was the best. At 0.5 A/g, the specific capacitance reached 333.50 F/g (three-electrode system) and 242.20 F/g (two-electrode system). After 5000 charge/discharge cycles at 5 A/g, the material maintained good cycling stability and achieved a capacitance retention rate of 95.14% (three-electrode system) and 97.04% (two-electrode system). The energy and power densities of the SNC700 samples were 8.33 Wh/kg and 62.5 W/kg at 0.25 A/g, respectively, values that meet the requirements of today's commercially available supercapacitor electrode materials, further demonstrating their good practicality. This paper provides an efficient double-doping method to prepare layered structures. Porous carbon is used for electrochemical energy storage devices.

Lignin-based composites for high-performance supercapacitor electrode materials.

With the rapid development of the global economy, the depletion of fossil fuels and the intensification of environmental pollution, there is an increasingly urgent need for new and green electrochemical energy storage technologies in society. In this thesis, ligninsulfonate/polyaniline nanocomposites were synthesized by in situ chemical oxidation using aniline as the monomer, lignin as the template and dopant, and ammonium persulfate as the oxidant. The results showed that the average diameter of the ligninsulfonate/polyaniline nanocomposite was 85 nm, and the composite electrode exhibited good electron conduction ability and excellent capacitive performance by ligninsulfonate doping. The electrode material showed the best electrochemical performance when the ligninsulfonate addition was 0.1 g. The specific capacitance can reach 553.7 F g-1 under the current density of charge/discharge 1 A g-1, which is higher than that of the pure PANI electrode. The composite electrode material has good multiplicative performance and cycling stability, and the capacitance retention rate can be maintained at 68.01% after 5000 cycles at a charge/discharge current density of 10 A g-1 (three-electrode system), and the capacitance retention rate can be maintained at 54.84% after 5000 cycles at a charge/discharge current density of 5 A g-1 (two-electrode system).

[The performance and applications of lignin based hydrogels: a review].

Lignin is the only natural aromatic polymer and the second most abundant lignocellulosic resource in nature after cellulose, and its derived high-value products can be used in a variety of fields. An efficient, highly value-added and high-quality production of lignin is critical for lignocellulose biorefinery, but the complex and variable structure of lignin macromolecules, poor activity of reactions, and redundant functional groups make it difficult to prepare polymeric materials with stable properties. With the increasing research on lignin modification, the application of lignin composite hydrogels has also received great attention. In this paper, the preparation of lignin composite hydrogels based on the basic structural composition and reaction properties of lignin was briefly outlined. The current applications of lignin composite hydrogels, including biosensors, controlled release materials, environmentally responsive materials, adsorbent materials, electrode materials, and other materials, were summarized. Moreover, future perspectives of lignin-based composite hydrogels were prospected.

Review on the preparation of fuels and chemicals based on lignin.

Lignin is by far the most abundant natural renewable aromatic polymer in nature, and its reserves are second only to cellulose. In addition to the rich carbon content, the structure of lignin contains functional groups such as benzene rings, methoxyl groups, and phenolic hydroxyl groups. Lignin degradation has become one of the high value, high quality and high efficiency methods to convert lignin, which is of great significance to alleviating the current energy shortage and environmental crisis. This article introduces the hydrolysis methods of lignin in acidic, alkaline, ionic liquids and supercritical fluids, reviews the heating rate, the source of lignin species and the effects of heating rate on the pyrolysis of lignin, and briefly describes the metal catalysis, oxidation methods such as electrochemical degradation and photocatalytic oxidation, and degradation reduction methods using hydrogen and hydrogen supply reagents. The lignin degradation methods for the preparation of fuels and chemicals are systematically summarized. The advantages and disadvantages of different methods, the selectivity under different conditions and the degradation efficiency of different catalytic combination systems are compared. In this paper, a new approach to improve the degradation efficiency is envisioned in order to contribute to the efficient utilization and high value conversion of lignin.

Review on the preparation and application of lignin-based carbon aerogels.

Carbon aerogels (CAs) are excellent carrier materials with a large surface area and high porosity. In addition to the above-mentioned wonderful characteristics, aerogel with lignin as a precursor is also a material with high bioactivity and degradability. Lignin carbon aerogels (LCAs) have a wide range of applications, and can be used in supercapacitors, adsorbents and catalysts, etc., but their preparation process is more complex. In this paper, we review the preparation and influencing factors of LCAs, analyze their properties and structural characterization, and aim to provide references for the optimal preparation, effective characterization, and expansion of applications of LCAs.

Correction: Genetic analysis of agronomic traits in elite sugarcane (Saccharum spp.) germplasm.

[This corrects the article DOI: 10.1371/journal.pone.0233752.].

Genetic analysis of agronomic traits in elite sugarcane (Saccharum spp.) germplasm.

Sugarcane (Saccharum spp.) is an important economic crop, supplying up to 80% of the table sugar and ~60% of bio-ethanol worldwide. Due to population growth and dwindling fossil-fuel reserves, the demand for sugar and bio-ethanol requires significant improvement in sugarcane production. Breeding sugarcane cultivars with high-performance agronomic traits is undoubtedly the most efficient way to achieve this goal. Therefore, evaluating agronomic traits and dissecting underlying loci are critically important for this aim steps in providing genetic resources and molecular markers for selection. In this study, we assembled a diversity panel of 236 elite sugarcane germplasms originally collected from 12 countries. We evaluated 28 agronomic traits in the diversity panel with three replicates. The diversity panel was genotyped using amplified fragment length polymorphism markers, and a total of 1,359 markers were generated. Through the genome-wide association study, we identified three markers significantly associated with three traits evaluated at a stringent threshold (P < 0.05 after Bonferroni correction). The genotypes of the three associated markers grouped respective trait values into two distinct groups, supporting the reliability of these markers for breeding selection. Our study provides putative molecular markers linked to agronomic traits for breeding robust sugarcane cultivars. Additionally, this study emphasized the importance of sugarcane germplasm introduced from other countries and suggested that the use of these germplasms in breeding programs depends on local industrial needs.

Two Decades' Variation of Trace Elements in Bones of the Endangered East Asian Finless Porpoise (Neophocaena asaeorientalis sunameri) from the East China Sea, China.

There has been increasing concern about trace element accumulation in finless porpoises over the past decades, while the long-term variation of its concentration has been little known. Since most soft tissues of porpoises in the past were not preserved until now, the bone tissue is the only ideal material available. Here, 27 East Asian finless porpoise bone samples collected from Nantong, the East China Sea during two periods (1984-1992 and 2009) were used to explore the temporal variation of nine typical elements' concentration (Fe, Mn, Cu, Ni, Hg, Cr, Pb, As, and Cd, dry weight). An upward trend for total trace element accumulation from 329.04 mg/kg in 1984-1992 to 1535.81 mg/kg in 2009 was found; this could have resulted from the increasing industrial development in the Nantong region. The Mn, Fe, and As levels increased significantly while the concentrations of Cd, Cu, and Pb significantly decreased. Generally, our results suggested that most trace elements posed low threats to East Asian finless porpoises' health during both periods, while elements Mn (2009), Ni (both periods), Fe (2009), and As (2009) posed a potentially higher health risk to porpoises. Thus, the trace element levels warrant concern and continuous monitoring.