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1.
Nat Commun ; 15(1): 4811, 2024 Jun 06.
Article in English | MEDLINE | ID: mdl-38844452

ABSTRACT

Human multidrug resistance protein 5 (hMRP5) effluxes anticancer and antivirus drugs, driving multidrug resistance. To uncover the mechanism of hMRP5, we determine six distinct cryo-EM structures, revealing an autoinhibitory N-terminal peptide that must dissociate to permit subsequent substrate recruitment. Guided by these molecular insights, we design an inhibitory peptide that could block substrate entry into the transport pathway. We also identify a regulatory motif, comprising a positively charged cluster and hydrophobic patches, within the first nucleotide-binding domain that modulates hMRP5 localization by engaging with membranes. By integrating our structural, biochemical, computational, and cell biological findings, we propose a model for hMRP5 conformational cycling and localization. Overall, this work provides mechanistic understanding of hMRP5 function, while informing future selective hMRP5 inhibitor development. More broadly, this study advances our understanding of the structural dynamics and inhibition of ABC transporters.


Subject(s)
Cryoelectron Microscopy , Humans , ATP-Binding Cassette Transporters/metabolism , ATP-Binding Cassette Transporters/chemistry , Biological Transport , HEK293 Cells , Models, Molecular , Multidrug Resistance-Associated Proteins/metabolism , Multidrug Resistance-Associated Proteins/chemistry , Multidrug Resistance-Associated Proteins/antagonists & inhibitors , Multidrug Resistance-Associated Proteins/genetics , Peptides/metabolism , Peptides/chemistry , Protein Conformation
2.
Commun Biol ; 7(1): 505, 2024 Apr 27.
Article in English | MEDLINE | ID: mdl-38678117

ABSTRACT

Alpha-fetoprotein (AFP), a serum glycoprotein, is expressed during embryonic development and the pathogenesis of liver cancer. It serves as a clinical tumor marker, function as a carcinogen, immune suppressor, and transport vehicle; but the detailed AFP structural information has not yet been reported. In this study, we used single-particle cryo-electron microscopy(cryo-EM) to analyze the structure of the recombinant AFP obtained a 3.31 Å cryo-EM structure and built an atomic model of AFP. We observed and identified certain structural features of AFP, including N-glycosylation at Asn251, four natural fatty acids bound to distinct domains, and the coordination of metal ions by residues His22, His264, His268, and Asp280. Furthermore, we compared the structural similarities and differences between AFP and human serum albumin. The elucidation of AFP's structural characteristics not only contributes to a deeper understanding of its functional mechanisms, but also provides a structural basis for developing AFP-based drug vehicles.


Subject(s)
Fatty Acids , Models, Molecular , alpha-Fetoproteins , Humans , alpha-Fetoproteins/metabolism , alpha-Fetoproteins/chemistry , Binding Sites , Cryoelectron Microscopy , Fatty Acids/metabolism , Glycosylation , Metals/metabolism , Metals/chemistry , Protein Conformation , Recombinant Proteins/metabolism , Recombinant Proteins/chemistry
3.
Cell Res ; 33(7): 533-545, 2023 07.
Article in English | MEDLINE | ID: mdl-37258749

ABSTRACT

Secretory-pathway Ca2+-ATPases (SPCAs) play critical roles in maintaining Ca2+ homeostasis, but the exact mechanism of SPCAs-mediated Ca2+ transport remains unclear. Here, we determined six cryo-electron microscopy (cryo-EM) structures of human SPCA1 (hSPCA1) in a series of intermediate states, revealing a near-complete conformational cycle. With the aid of molecular dynamics simulations, these structures offer a clear structural basis for Ca2+ entry and release in hSPCA1. We found that hSPCA1 undergoes unique conformational changes during ATP binding and phosphorylation compared to other well-studied P-type II ATPases. In addition, we observed a conformational distortion of the Ca2+-binding site induced by the separation of transmembrane helices 4L and 6, unveiling a distinct Ca2+ release mechanism. Particularly, we determined a structure of the long-sought CaE2P state of P-type IIA ATPases, providing valuable insights into the Ca2+ transport cycle. Together, these findings enhance our understanding of Ca2+ transport by hSPCA1 and broaden our knowledge of P-type ATPases.


Subject(s)
Calcium-Transporting ATPases , Calcium , Humans , Calcium/metabolism , Cryoelectron Microscopy , Calcium-Transporting ATPases/metabolism , Adenosine Triphosphatases/metabolism
4.
Nat Methods ; 20(1): 123-130, 2023 01.
Article in English | MEDLINE | ID: mdl-36522503

ABSTRACT

Cryo-electron microscopy (cryo-EM) visualizes the atomic structure of macromolecules that are embedded in vitrified thin ice at their close-to-native state. However, the homogeneity of ice thickness, a key factor to ensure high image quality, is poorly controlled during specimen preparation and has become one of the main challenges for high-resolution cryo-EM. Here we found that the uniformity of thin ice relies on the surface flatness of the supporting film, and developed a method to use ultraflat graphene (UFG) as the support for cryo-EM specimen preparation to achieve better control of vitreous ice thickness. We show that the uniform thin ice on UFG improves the image quality of vitrified specimens. Using such a method we successfully determined the three-dimensional structures of hemoglobin (64 kDa), α-fetoprotein (67 kDa) with no symmetry, and streptavidin (52 kDa) at a resolution of 3.5 Å, 2.6 Å and 2.2 Å, respectively. Furthermore, our results demonstrate the potential of UFG for the fields of cryo-electron tomography and structure-based drug discovery.


Subject(s)
Graphite , Cryoelectron Microscopy/methods , Graphite/chemistry , Macromolecular Substances , Electron Microscope Tomography
5.
Biochem Biophys Res Commun ; 636(Pt 1): 121-124, 2022 12 25.
Article in English | MEDLINE | ID: mdl-36332473

ABSTRACT

IGF1R plays an important role in regulating cellular metabolism and cell growth, and has been identified as an anti-cancer and diabetes drug target. Although research have been reported many crystal and cryo-EM structures of IGF1R, the mechanism of ligand binding remains controversial, mainly because the structure differences among its cryo-EM, crystal and homologous protein insulin receptor structures. Here, we further determined one new high-resolution symmetric cryo-EM structure of ligand-bound IGF1R and be the first to prove that the receptor could bind to two IGFI molecules by single particle cryo-electron microscopy. And the structure is very different from its homologous protein insulin receptor: the two ligands just exist at the binding site 2 with saturating ligand conditions. Then, our findings resolved the major dispute about the comformational changes of IGF1R, and proposed a new theory how IGF1R binds to its ligands. Meanwhile, these findings imply more attention may be needed to study the relationship between the special conformation and their corresponding physiological functions in future.


Subject(s)
Insulin-Like Growth Factor I , Receptor, IGF Type 1 , Humans , Cryoelectron Microscopy , Hormones , Insulin-Like Growth Factor I/chemistry , Ligands , Protein Domains , Receptor, IGF Type 1/chemistry , Receptor, Insulin/chemistry
6.
Biochem Biophys Res Commun ; 624: 89-94, 2022 10 08.
Article in English | MEDLINE | ID: mdl-35940132

ABSTRACT

The human VPS10 domain-containing receptor SorCS3 belongs to the Vps10p-domain receptor family and is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the cryo-EM structure of the full-length SorCS3 receptor and further found that there were at least three distinct conformations (monomer, M-shaped dimer and N-shaped dimer) of SorCS3 in the apo state. The differences between the two dimer conformations were caused by PKD1-2 assembly. In contrast to its homologous proteins, the conserved residues GLN198, ARG678, TYR430, GLU1020 and ASP1024 may be key points for its dimerization and for protein/polypeptide binding. These results showed the structural details of apo-SorCS3, which provides a foundation for elucidating the mechanism of protein sorting.


Subject(s)
Carrier Proteins , Nerve Tissue Proteins , Carrier Proteins/metabolism , Cryoelectron Microscopy , Humans , Nerve Tissue Proteins/metabolism , Protein Binding , Protein Transport , Receptors, Cell Surface/metabolism
7.
Biochem Biophys Res Commun ; 618: 148-152, 2022 08 27.
Article in English | MEDLINE | ID: mdl-35749888

ABSTRACT

IGF1R plays an important role in regulating cellular metabolism and growth. As a single transmembrane protein, its structure is flexible. Although previous studies revealed some structures of IGF1R, the cryo-EM apo structures of the receptor have never been reported. Herein, we reported four distinct cryo-EM structures that reveal the apo states of IGF1R. These conformations were classified as "Resting states" and "Active states", according to the orientation of α-CT helices and structural symmetry. In addition, a "Ligand-pocket" was formed in the active conformations, which presented a new view of conformational changes of apo-IGF1R. These results suggest a new dynamic change model to show the details of why and how ligands can bind to IGF1R.


Subject(s)
Receptor, IGF Type 1 , Cryoelectron Microscopy/methods , Humans , Ligands , Protein Conformation , Receptor, IGF Type 1/chemistry , Receptor, IGF Type 1/ultrastructure
8.
J Mol Biol ; 434(9): 167536, 2022 05 15.
Article in English | MEDLINE | ID: mdl-35300993

ABSTRACT

Type 1 insulin-like growth factor receptor (IGF1R) plays an important role in regulating cellular metabolism and cell growth and has been identified as an anticancer drug target. Although previous studies have revealed some structures of IGF1R with different ligands, the continuous dynamic conformation change remains unclear. Here, we report 10 distinct structures (7.9-3.6 Å) of IGF1R bound to IGF1 or insulin to reveal the polymorphic conformations of ligand-bound IGF1R. These results showed that the α-CT2, disulfide bond (C670-C670'), and FnIII-2 domains had the most flexible orientations for the conformational change that occurs when ligands bind to the receptor. In addition, we found one special conformation (tentatively named the diverter-switch state) in both complexes, which may be one of the apo-IGF1R forms under ligand-treatment conditions. Hence, these results illustrated the mechanism of how different ligands could bind to human IGF1R and provided a rational template for drug design.


Subject(s)
Insulin , Receptor, IGF Type 1 , Cell Proliferation , Cryoelectron Microscopy , Humans , Insulin/metabolism , Ligands , Protein Domains , Receptor, IGF Type 1/chemistry , Receptor, IGF Type 1/metabolism
9.
Biochem Biophys Res Commun ; 600: 75-79, 2022 04 16.
Article in English | MEDLINE | ID: mdl-35196630

ABSTRACT

Sorting-related receptor with A-type repeats (SORLA) is an important receptor for regulating normal cellular functions via protein sorting. Here, we determined the structures of the full-length SORLA and identified two distinct conformations of apo-SORLA using single-particle cryogenic electron microscopy. In contrast to homologous proteins, both monomer and dimer forms of SORLA existed in a neutral solution. Only three hydrogen bonds in the vicinity of the dimer interface implied the involvement in dimerization. The orientation of residue R490 was a key point for ligand binding. These results suggest a unique mechanism of SORLA dimerization for protein trafficking.


Subject(s)
Adaptor Proteins, Vesicular Transport , LDL-Receptor Related Proteins , Adaptor Proteins, Vesicular Transport/metabolism , Carrier Proteins/metabolism , Cryoelectron Microscopy , LDL-Receptor Related Proteins/metabolism , Protein Transport
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