Determining the initial impact of rear-end collisions by trace evidence left on the vehicle from tires: A case report.

If an automobile happens to crash into the back of another vehicle while travelling at high speeds, both vehicles will be seriously damaged. Consequently, it is not easy to reconstruct the initial collision state between the two vehicles or determine whether or not the risk perception of the driver is normal. The entire picture of the accident cannot be fully understood and thus clarifying the relevant legal responsibility is difficult. The trace evidence of tires, such as pattern, direction, and impression examination as well as other characteristics, can be carefully observed and used as evidence in accident reconstruction. A case report of a fatal collision involving a bus crashing into the frame of a full trailer on a freeway is examined in this study. The police agency used the characteristics of the trace evidence of the bus tires to reconstruct the initial collision state of the two vehicles to clarify the cause of the accident, and these determination guidelines can be used by police while handling similar cases in the future. This case uses new information regarding the initial collision state of road traffic accidents for reconstruction and provides knowledge and interest for the forensic community.

Unstable pelvic fractures associated with femoral shaft fractures: a retrospective analysis.

BACKGROUND: Both pelvic fractures and femoral shaft fractures are caused by high-energy injuries. When unstable pelvic fractures and femoral shaft fractures occur concomitantly, the optimal treatment method is controversial. The aim of this study was to establish a reasonable principle for treating such complicated injuries. METHODS: Forty patients sustaining unstable pelvic fractures and concomitant femoral shaft fractures were treated in a 7-year period. The initial management of the fractures was started at the emergency service according to the Advanced Trauma Life Support protocol. Unstable pelvic fractures were wrapped by cloth sheets and femoral shaft fractures were immobilized with a splint. Angiography was performed on patients with unstable hemodynamic status. The definitive treatment for combined fractures was performed after stabilizing the hemodynamics. Closed nailing was used for femoral shaft fractures, and pelvic fractures were treated with various techniques. RESULTS: The mortality rate was 12.5% (5/40) during admission. Thirty-three patients were followed up for an average of 32 months (range, 12-76 months). There were 33 cases of unstable pelvic fractures and 36 instances of femoral shaft fractures. The union rate for pelvic fractures was 100% (33/33), while femoral shaft fractures had a 94.4% (34/36) union rate. The average healing time was 3.3 months (range, 1.6-8.1 months) and 4.1 months (range, 2.5-18.2 months) for pelvic and femoral shaft fractures, respectively. After fracture, 34 hips (94%) achieved a satisfactory result in the Harris hip score and 30 knees (83%) achieved a satisfactory result in the Mize knee score. CONCLUSIONS: Stabilization of the hemodynamics in patients with combined fractures should be the first aim. Angiography to stop arterial bleeding in the pelvis is often life-saving. The definitive treatment for combined fractures, such as pelvic fractures and femoral shaft fractures, should wait until hemodynamics is stabilized.

Molecular cloning and characterization of a cytosolic manganese superoxide dismutase (cytMnSOD) and mitochondrial manganese superoxide dismutase (mtMnSOD) from the kuruma shrimp Marsupenaeus japonicus.

A cytosolic manganese superoxide dismutase (cytMnSOD) gene and a mitochondrial manganese superoxide dismutase (mtMnSOD) gene were cloned from the kuruma shrimp Marsupenaeus japonicus using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of cytMnSOD is 861 bp and encodes a 287 amino acids (aa) protein with a 61 aa leader sequence, whereas the ORF of mtMnSOD is 663 bp and encodes a 221 aa protein with a 21 aa mitochondrial-targeting sequence in the N-terminus. The calculated molecular mass of translated protein of cytMnSOD and mtMnSOD is 31.4 kDa and 24.3 kDa with an estimated pI of 5.62 and 7.27, respectively. The deduced amino acid sequence of cytMnSOD has similarity of 50.2% to that of mtMnSOD. Both cytMnSOD and mtMnSOD contain a manganese superoxide dismutase domain (DVWEHAYY), and four conserved amino acids responsible for binding manganese. Both cytMnSOD and mtMnSOD of M. japonicus were expressed in haemocytes, eyestalk, muscle, intestine, gill, and hepatopancreas. Both cytMnSOD and mtMnSOD transcripts in haemocytes of M. japonicus significantly increased 6 h after injection of Vibrio alginolyticus, and 12 h after injection of beta-glucan, indicating induction of SOD system response in a short time.

Community-based randomized intervention trial for periodontal disease after 18-month follow-up [Keelung Community-based Integrated Screening (KCIS) No. 4].

OBJECTIVES: As periodontal disease preponderates in adults, early detection and interventional regime is urgently needed. However, there is lack of evidence-based data on evaluation of population-based intervention programme related to periodontal disease. The aim of this study was to assess the efficacy of intervention regime on early periodontal disease identified from a community-based periodontal survey. METHOD: By randomization, 60 subjects were allocated to the intervention group and 49 to the control group respectively. Status on periodontal disease of participants was evaluated at entry and re-evaluated after intervention at 1-month and 18-month follow-up respectively. Primary outcome evaluated was based on Community Periodontal Index and Loss of Attachment on sextant level. RESULTS: The efficacy of intervention was significant in Community Periodontal Index (P<0.001) but not in Loss of Attachment (P=0.53) at 1-month and 18-month follow-up. CONCLUSION: The improvement of periodontal disease owing to intervention regime was seen in Community Periodontal Index but not Loss of Attachment as yet at 18-month follow-up after intervention.