ABSTRACT
Jolkinolide B (JB) and 17-hydroxy-JB (HJB) are diterpenoids from plants and it has been reported that the presence of a C-17 hydroxy group in JB significantly enhances the anti-inflammatory potency of JB. In this study, two HJB derivatives HJB-1 and HJB-2 were generated by the chemical modification of a 17-hydroxy group of HJB. HJB-1 more effectively inhibited TNF-α, IL-1ß and IL-6 release in LPS-stimulated mouse peritoneal macrophages. In addition, HJB-1 reduced LPS-induced mRNA expression of TNF-α, IL-1ß, IL-6, COX-2 and iNOS in a concentration-dependent manner, but did not alter IL-10 mRNA expression. LPS-induced NF-κB activation and MAPK phosphorylation were also effectively inhibited by HJB-1. These results demonstrate that HJB-1 exerts anti-inflammatory effects on LPS-activated mouse peritoneal macrophages by inhibiting NF-κB activation and MAPK phosphorylation and modification of a 17-hydroxy group of HJB may enhance the anti-inflammatory potency of HJB derivatives.
Subject(s)
Anti-Inflammatory Agents/pharmacology , Diterpenes/pharmacology , Inflammation/chemically induced , Inflammation/drug therapy , Lipopolysaccharides/adverse effects , Macrophages, Peritoneal/drug effects , Animals , Cyclooxygenase 2/metabolism , Inflammation/metabolism , Interleukin-10/metabolism , Interleukin-1beta/metabolism , Interleukin-6/metabolism , Macrophages, Peritoneal/metabolism , Mice , Mitogen-Activated Protein Kinases/metabolism , NF-kappa B/metabolism , Nitric Oxide Synthase Type II/metabolism , Phosphorylation/drug effects , Tumor Necrosis Factor-alpha/metabolismSubject(s)
Disease Outbreaks , Enterovirus A, Human/genetics , Hand, Foot and Mouth Disease/epidemiology , RNA, Viral/analysis , Child, Preschool , China/epidemiology , Cities/epidemiology , Enterovirus A, Human/classification , Enterovirus A, Human/isolation & purification , Hand, Foot and Mouth Disease/virology , Humans , Phylogeography , Reverse Transcriptase Polymerase Chain ReactionABSTRACT
Telomerase is Ribonucleoprotein complex in eukaryocyte, which is composed of telomerase reverse transcriptase(TERT) and telomerase RNA. Telomerase is a special DNA polymerase which can extend the terminal of DNA and maintain the length of telomere. TERT have reverse transcriptase activity. Telomerase activity do not examine in most somatic cell and primary cell, but most tumor cell have strong telomerase activity. It was think that the telomerase has strong relation with tumor occurrences. In this article,the author instruct the correlation of G-strand and P53 in tumor occurrences.
