ABSTRACT
A 24GHz Doppler radar system for accurate contactless monitoring of heart and respiratory rates is demonstrated here. High accuracy predictions are achieved by employing a CNN+LSTM neural network architecture for regression analysis. Detection accuracies of 99% and 98% have been attained for heart rate and respiration rate, respectively.Clinical Relevance- This work establishes a non-contact radar system with 99% detection accuracy for a heart rate variability warning system. This system can enable convenient and fast monitoring for daily care at home.
Subject(s)
Algorithms , Neural Networks, Computer , Heart Rate , Respiration , Respiratory RateSubject(s)
Amino Acids, Diamino/chemistry , Disulfides/chemical synthesis , Peptides/chemical synthesis , Antimicrobial Cationic Peptides/chemistry , Antimicrobial Cationic Peptides/pharmacology , Bacteria/drug effects , Chromatography, High Pressure Liquid , Circular Dichroism , Cyclization , DNA-Binding Proteins/chemistry , DNA-Binding Proteins/pharmacology , Disulfides/chemistry , Disulfides/pharmacology , Microbial Sensitivity Tests , Molecular Structure , Peptides/chemistry , Peptides/pharmacology , Peptides, Cyclic/chemistry , Peptides, Cyclic/pharmacology , Solid-Phase Synthesis TechniquesABSTRACT
This study evaluated the clinical efficacy of autologous cytokine-induced killer (CIK) cell transfusion in combination with transcatheter arterial chemoembolization (TACE) and radiofrequency ablation (RFA), compared to sequential therapy with TACE and RFA, for the treatment of hepatocellular carcinoma (HCC). We retrospectively studied 2 groups of HCC patients: 85 patients in the TACE+RFA+CIK group were treated with adoptive autologous CIK cell transfusion in combination with minimally invasive therapy, 89 patients in the TACE+RFA group were treated with minimally invasive therapy alone. The overall response rate was 76.5% in the TACE+RFA+CIK group and 79.8% in the TACE+RFA group. The disease control rate was higher in the TACE+RFA+CIK group than that in the TACE+RFA group (95.3% vs. 88.8%), but the difference was not significant (P=0.113). Kaplan-Meier analysis showed that the patients in the TACE+RFA+CIK group had significantly longer overall survival (56 vs. 31 mo, P=0.001) and progression-free survival (17 vs. 10 mo, P=0.001) than those in the TACE+RFA group. No severe side effects occurred in the CIK cell transfusion patients. In conclusion, CIK cell immunotherapy may be a valuable therapeutic strategy to prevent recurrence and metastasis in HCC patients after TACE and RFA, and to improve patient prognosis and quality of life. Combined CIK immunotherapy and minimally invasive therapies represent a safe, potential treatment modality for HCC. However, because patient assignment to the 2 treatments was not randomized, any conclusions concerning improvements in survival must be interpreted with great caution.
Subject(s)
Carcinoma, Hepatocellular/therapy , Cytokine-Induced Killer Cells/transplantation , Immunotherapy, Adoptive/methods , Liver Neoplasms/therapy , Adult , Aged , Aged, 80 and over , Carcinoma, Hepatocellular/mortality , Carcinoma, Hepatocellular/pathology , Catheter Ablation , Catheterization, Peripheral , Chemoembolization, Therapeutic , Combined Modality Therapy , Cytokine-Induced Killer Cells/pathology , Disease-Free Survival , Female , Follow-Up Studies , Humans , Liver Neoplasms/mortality , Liver Neoplasms/pathology , Male , Middle Aged , Retrospective Studies , Young AdultABSTRACT
This work experimentally demonstrates the efficacy of a radio-frequency phase shifter using a distributed feedback laser in a microwave transport system. Phase shifts of about 101 degrees are obtained at 8.75 GHz. The proposed phase shifter can amplify microwave signals and thereby improve transmission performance. Additionally, a similar single sideband modulation can be generated by the phase shifter. Experimental results indicate that the proposed phase shifter can be used in future long-distance microwave transport systems and all optical inverters.
Subject(s)
Lasers , Telecommunications/instrumentation , Computer-Aided Design , Equipment Design , Equipment Failure Analysis , Feedback , Microwaves , Reproducibility of Results , Sensitivity and SpecificityABSTRACT
The gpr81 was amplified by polymerase chain reaction (PCR) using human fetus kidney cDNA and whole blood genome DNA as template, respectively. The expression profile of gpr81 in human fetus was analyzed by RT-PCR and the result indicated GPR81 mRNA was most abundant in fetus liver and heart. In addition, the deduced amino acid of GPR81 was compared with other related molecules by Clustal w/x software, and a molecular phylogenetic tree was constructed with Treeview software. It was showed that GPR81 had the highest homology with nicotinic acid receptor in amino acids. After sequence identification, gpr81 was inserted into the plasmid pcDNA3. 1 (-)/his-mycA and then transfected into Chinese hamster ovary cell (CHO-K1). With the selection of G418, an engineered cell line which could stably express gpr81 was obtained by the indication of RT-PCR and Western-blot detection. The establishment of the cell line will serve as means for further study of GPR81.
Subject(s)
Fetus/metabolism , Receptors, G-Protein-Coupled/genetics , Amino Acid Sequence , Animals , CHO Cells , Cloning, Molecular , Cricetinae , Cricetulus , DNA, Complementary/genetics , Gene Expression Profiling , Humans , Molecular Sequence Data , Phylogeny , Receptors, G-Protein-Coupled/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Transcription, Genetic , TransfectionABSTRACT
As a member of orphan G protein-coupled receptors (oGPCRs), hGPCRc was cloned from human colon tissue and analyzed by bioinformatic softwares. It was showed that the corresponding amino acids of hGPCRc formed seven-transmembrane domains as the key characteristic of GPCRs. Then, the recombinant GFP-hGPCRc was constructed by fussing hGPCRc into pEGFP-N1 carrying green fluorescent protein (GFP) gene, and CHO-K1 cells were subsequently transfected with the GFP-hGPCRc or pEGFP-N1. The green fluorescence protein expression in the two different transfected cells was observed under the laser scanning confocal microscopy (LSCM). It was showed that green fluorescence protein was distributed in the whole bodies of the cells transfected with pEGFP-N1, but mainly distributed on the plasma membrane and cytoplasm membrane transfected with GFP-hGPCRc. Thus, the localization on the membrane of hGPCRc was accorded with the predication by bioinformatic analysis. The expression analysis of hGPCRc by RT-PCR indicated that hGPCRc was abundantly expressed in heart, kidney, cerebel and colon etc., but absent in liver, cerebra, small intestine and muscle etc. The expressing profile of hGPCRc could provide some useful clues to understanding its effects on embryonic development and physiological functions.
