ABSTRACT
To study the chemical composition of the skin of Bufo bufo gargarizans, various chromatographic methods were used in the isolation procedures and the structures of isolated compounds were determined based on NMR and MS analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as N-[2-hydroxy-2-(4-hydroxyphenyl)ethyl]-N-methylurea (1) and 19-oxocinobufotalin 3-adipoylarginine ester (2), together with 11 known compounds. Isolated bufadienolides showed significant inhibition effect against human hepatocellular carcinoma cell line SMMC-7721 in vitro.
Subject(s)
Antineoplastic Agents/isolation & purification , Antineoplastic Agents/pharmacology , Arginine/analogs & derivatives , Bufanolides/isolation & purification , Bufanolides/pharmacology , Bufonidae , Methylurea Compounds/isolation & purification , Methylurea Compounds/pharmacology , Skin/chemistry , Animals , Antineoplastic Agents/chemistry , Arginine/chemistry , Arginine/isolation & purification , Arginine/pharmacology , Bufanolides/chemistry , China , Drug Screening Assays, Antitumor , Humans , Methylurea Compounds/chemistry , Molecular Structure , Nuclear Magnetic Resonance, BiomolecularABSTRACT
To study the chemical composition of the skin of Bufo bufo gargarizans, many kinds of chromatography methods were used in the isolation procedures, while the structures of isolated compounds were determined on the basis of their NMR and MS spectral analysis. As a result, two new compounds were isolated from its ethanolic extract and characterized as cinobufotalin 3-nonanedioylarginine ester (8) and bufotalin 3-pimeloylarginine ester (14). Furthermore, 13 known compounds were obtained. Isolated bufadienolides showed significant inhibition effect against SMMC-7721 cell lines in vitro.
