ABSTRACT
Small active RNA (saRNA)-induced gene activation (RNAa) is a novel strategy to treat cancer. Our previous work proved that the p21-saRNA-322 successfully hindered colorectal cancer growth by activating p21 gene. However, the barrier for successful saRNA therapy is lack of efficient drug delivery. In the present study, a rectal delivery system entitled p21-saRNA-322 encapsulated tumor-selective lipopolyplex (TSLPP-p21-saRNA-322) which consist of PEI/p21-saRNA-322 polyplex core and hyaluronan (HA) modulated lipid shell was developed to treat colorectal cancer. Our results showed that this system maintained at the rectum for more than 6 h and preferentially accumulated at tumor site. CD44 knock down experiment instructed that the superb cellular uptake of TSLPP-p21-saRNA-322 attributed to HA-CD44 recognition. An orthotopic model of bio-luminescence human colorectal cancer in mice was developed using microsurgery and TSLPP-p21-saRNA-322 demonstrated a superior antitumor efficacy in vitro and in vivo. Our results provide preclinical proof-of-concept for a novel method to treat colorectal cancer by rectal administration of TSLPP formulated p21-saRNA-322.
Subject(s)
Colorectal Neoplasms/therapy , Cyclin-Dependent Kinase Inhibitor p21/genetics , Gene Transfer Techniques , Genetic Therapy/methods , RNA/therapeutic use , Transcriptional Activation , Animals , Cell Cycle , Colon/metabolism , Colon/pathology , Colorectal Neoplasms/genetics , Colorectal Neoplasms/pathology , Drug Delivery Systems/methods , HT29 Cells , Humans , Hyaluronan Receptors/genetics , Hyaluronic Acid/analogs & derivatives , Lipids/chemistry , Male , Mice , Mice, Inbred NOD , Mice, SCID , RNA/administration & dosage , RNA/genetics , Rectum/metabolism , Rectum/pathologyABSTRACT
Seven metabolites of 2',3',5'-tri-O-acetyl-N6-(3-hydroxyphenyl) adenosine (WS070117) were synthesized by deacetylation, hydrolysis, cyclization, sulfonylation and glycosylation reactions, respectively. All these compounds, which could be useful as material standards for metabolic research, were characterized by NMR and HPLC-MS (ESI) analyses.
