ABSTRACT
The present study was designed to isolate and characterize the analgesic compounds of Artemisa sacrorum Ledeb. The EtOAc crude extracts from the aerial parts of Artemisa sacrorum Ledeb were separated by chromatography and the structures of new compounds were elucidated based on spectral analyses. Analgesic activities of the isolated compounds were assessed in rats with hot plate test and paw pressure assay. Two new flavone C-glycosides, named as Sacroroside A and B (Compounds 1 and 2) were isolated from the EtOAc crude extract of the aerial parts ofArtemisa sacrorum Ledeb. They showed significant analgesic effects. In conclusion, Compounds 1 and 2 are new natural products, which show significant analgesic effects in a dose-dependent manner.
Subject(s)
Analgesics/therapeutic use , Artemisia/chemistry , Disaccharides/therapeutic use , Flavanones/therapeutic use , Flavones/therapeutic use , Pain/drug therapy , Phytotherapy , Plant Extracts/therapeutic use , Analgesics/chemistry , Analgesics/isolation & purification , Analgesics/pharmacology , Animals , Disaccharides/chemistry , Disaccharides/isolation & purification , Disaccharides/pharmacology , Flavanones/chemistry , Flavanones/isolation & purification , Flavanones/pharmacology , Flavones/chemistry , Flavones/isolation & purification , Flavones/pharmacology , Hot Temperature , Male , Molecular Structure , Plant Components, Aerial , Plant Extracts/chemistry , Plant Extracts/pharmacology , Pressure , Rats, WistarABSTRACT
OBJECTIVE: To study the chemical constituents of the ethyl acetate extract from Panzeria alaschanica. METHODS: The chemical constituents of ethyl acetate extract from Panzeria alaschanica were isolated and purified by silica gel. Their structures were i- dentified by means of spectra. RESULTS: Nine compounds were obtained and identified as 7-Methoxy coumarin (1), Isorhamnetin (2), Caf- feic acid (3), 5-Hydroxy-7,3',4'-trimethoxyflavone (4), 5-Hydroxy-7,4'-dimethoxyflavone (5),Kaempferol (6), Isorhamnetin-3-O-ß-D- glucoside (7) Kaempferol-3-O-ß-D-glucoside (8), and Isorhamnetin-7-O-ß-D-glucuronyl-(1-->6)-O-α-L-rhamnoside (9). CONCLUSION: Compounds 1-4,6,7 and 9 are isolated from this plant for the first time.