Autotetraploid rice: challenges and opportunities.

Autotetraploid rice is a type of germplasm developed from the whole genome duplication of diploid rice, leading to large grains, high nutrient content, and resistance. However, its low fertility has reduced yield and hampered commercialization. To address this issue, a new type of high fertility tetraploid rice was developed, which may serve as a useful germplasm for polyploid rice breeding. In this review, we summarize the progress made in understanding the cellular and molecular genetic mechanisms underlying the low fertility of autotetraploid rice and its F1 hybrid, as well as the main types of new tetraploid rice with high fertility. Lastly, the idea of utilizing the multi-generation heterosis of neo-tetraploid rice in the future is proposed as a reference for polyploid rice breeding.

[Identification and characteristics of multiple agrometeorological disaster of maize in Liaoning Province, China]. / 辽宁省玉米复合农业气象灾害判识及特征.

Multiple agrometeorological disaster (MAD) occurs simultaneously in maize production. In order to ascertain the occurrence regularity and characteristics of MAD of maize in Liaoning Pro-vince, we defined and classified MAD, identified MAD of 50 meteorological stations in the maize growing season of Liaoning Province from 1961 to 2017, and examined the effects of MAD on maize yield in typical years. The results showed that the occurrence range of MAD was higher in most years from 1961 to 2017 than that of single agrometeorological disaster (SAD), and that the occurrence frequency of MAD was higher in most areas than that of SAD. The major MAD in Liaoning Province was drought in multiple periods (M1-D), followed by the combination of drought and delayed cold damage (M2-DC). The occurrence range of M1-D showed an upward trend from 1961 to 2017, whereas other MAD types showed a downward trend. After analyzing the occurrence of MAD in the typical years of maize yield reduction, we found that the occurrence frequency of M1-D and M2-DC was higher.

[Effect of climatic mean value change on the evaluation result of rice delayed cold damage in Liaoning Province, Northeast China].

The anomaly of mean temperature summation from May to September (ΔT5-9) was commonly used to assess delayed cold damage of rice in Northeast China, but whether the change of statistics years for climatic mean value (ΣT5-9) would affect the, evaluation results of Liaoning rice under cold damage needed to be further studied. By using the meteorological industry standard of the People's Republic of China "technical standard on rice cold damage evaluation" (QX/T 182- 2013) and the supplemental indices (ΔT5-9), the index (ΣT5-9) was calculated in four periods 1961-1990 (S1), 1971-2000 (S2), 1981-2010 (S3) and 1961-2010 (S4), and the spatial and temporal changes of cold damage in Liaoning Province were analyzed based on the ratio between cold damage stations and total stations (IOC) and the occurrence frequency. The results showed that the heat condition (Σ T5-9) in rice growing season increased obviously and the spatial and temporal changes were significant from 1961 to 2010. The original meteorological index of rice cold damage was improved by using quadratic polynomial model. The identification results were similar between S2 and S4. The variation coefficient of IOC in S3 was lower than that of the other three. Compared with the typical rice yield reduction years, the evaluation results accorded better with the actual situation in evaluating the rice delayed cold damage in Liaoning during study period by using the S3 climate mean value. The results could provide evidence for accurately evaluating the variation of rice cold damage in spatial and temporal distribution in Liaoning Province under the background of global climate change.

[Spatiotemporal dynamics of maize water suitability and assessment of agricultural drought in Liaoning Province, China from 1981 to 2010].

Maize water suitability (MWS) model was developed at growth stage scale. Frequency and severity of drought were evaluated by combining MWS estimates and agricultural meteorological drought indexes. The MWS at each growth stage was calculated by using maize observational data and conventional meteorological data at 52 sites in Liaoning during the period from 1981 to 2010. Based on the climatic trend and abrupt change analysis, spatiotemporal dynamics of MWS were investigated. Meanwhile, occurrence of agricultural drought and its severity were also estimated. The results showed that the variation of MWS largely differed at different growth stages. Climatic abrupt change happened in 1994, 1996 and 1999 at the stages of emergence to seven leaves (II), jointing to tasseling (IV) and physiological maturity to maturity (VI). During the past 30 years, MWS showed an obvious increasing trend at the stages of sowing to emergence(I) , seven leaves to jointing(III), IV and tasseling to physiological maturity(V), while it showed a decreasing trend at the stages of II and VI, and that at VI stage was statistically significant. In addition, the climatic trend of MWS showed apparently spatial variability. The frequencies of drought at different severities varied with maize growth stages. Areas of high variability of MWS were located in the Northwest and South of Liaoning at the stages of I , II , III and VI, where were also the regions of high frequency of mid- and severe-drought. At the stages of IV and V, the frequency of drought was low and only light- and mid-drought occurred in few areas. In conclusion, the regional mean MWS could be capable to reasonably assess the agricultural drought in Liaoning at the regional scale.

Analysis on genetic diversification and heterosis in autotetraploid rice.

Polyploidization has played an important role in plant evolution and is a pathway for plants to increase genetic diversification and to get higher heterosis comparing with that of diploid does. This study was undertaken to assess the genetic variation and relationships among 40 autotetraploid rice genotypes and their counterpart diploid cultivars with 99 SSR markers screened from published rice genome. The 99 SSR markers detected polymorphism among autotetraploid genotypes and revealed a total of 291 alleles with an average of 2.949 alleles per locus. Autotetraploid lines showed higher genetic diversity and significant variation in agronomic traits than diploid cultivars. Phylogenetic analysis revealed that most of autotetraploid lines were genetically different from their diploid parents, and inter-subspecific hybrids were prepared on the basis of genetic distance between parents. Inter-subspecific autotetraploid hybrids showed a higher and positive heterobeltiosis and competitive heterosis than diploid hybrids, especially for grain yield. Genetic distance appeared not to predict heterosis in diploid rice for all traits; however, it showed a significant correlation with grain yield, grain length and grain length to width ratio in autotetraploid rice. This extensive research on autotetraploid heterosis and genetic diversity will be useful for the development of autotetraploid rice hybrids.

[Urban heat island effect based on urban heat island source and sink indices in Shenyang, Northeast China].

Based on the remote images in 2001 and 2010, the source and sink areas of urban heat island (UHI) in Shenyang City, Northeast China were determined by GIS technique. The effect of urban regional landscape pattern on UHI effect was assessed with land surface temperature (LST), area rate index (CI) of the source and sink areas and intensity index (LI) of heat island. The results indicated that the land use type changed significantly from 2001 to 2010, which significantly changed the source and sink areas of UHI, especially in the second and third circle regions. The source and sink areas were 94.3% and 5.7% in the first circle region, 64.0% and 36.0% in the third circle region in 2001, while they were 93.4% and 6.6%, 70.2% and 29.8% in 2010, respectively. It suggested that the land use pattern extended by a round shape in Shenyang led to the corresponding UHI pattern. The LST in the study area tended to decrease from the first circle region to the third. The UHI intensity was characterized with a single center in 2001 and with several centers in 2010, and the grade of UHI intensity was in a decreasing trend from 2001 to 2010. The absolute value of CI increased from the first circle region to the third, and the L1 was close to 1, suggesting the change in land use pattern had no significant influence on UHI in Shenyang.

[Spectral characteristics of Pinus tabulaeformis canopy with different damaged rates of needle leaf in western Liaoning Province, Northeast China].

Through the measurement of the spectral reflectance of large areas Chinese pine (Pinus tabulaeformis Carr. ) canopy in western Liaoning Province, this paper analyzed the difference of the spectral reflectance of the canopies with different damaged rates of needle leaf. In visible band, the characteristics of the spectral reflectance of P. tabulaeformis canopies with healthy and damaged needle leaf were in accordance with the spectral characteristics of green plants, but the position of red valley was not obvious when the damaged rate of needle leaf was higher than 60%. In near-infrared band, with the decrease of the damaged rate of needle leaf, the canopy spectral reflectance increased at 780-1350 nm, but decreased at 1450-1800 and 1950-2350 nm. With the increase of the damaged rate, the position of red-edge inflection moved to the short-wave direction. There were significant correlations between the damaged rate of needle leaf and the red edge feature variables and some vegetation indices. The model based on DVI (1470, 860) could be more reliable for predicting the damaged rate of needle leaf in P. tabulaeformis canopy in western Liaoning Province.

[Hyperspectral characteristics of spring maize from jointing to silking stage under drought stress].

By monitoring hyperspectral characteristics of spring maize from jointing to silking stage under different drought stress in Jinzhou of Liaoning, China, its spectral characteristics of visible light, red edge region and near infrared were researched, and the correlation of spectral reflectivity of different wavelength with soil moisture was analyzed. The results show that during the jointing to siking stage, the spectral reflectivity of 350-710 nm has significant negative correlation to soil moisture of 0 and 20 cm depth, but the spectral reflectivity of 710-1 300 nm has positive correlation to soil moisture of 0, 20, 40 and 60 cm, and the significant correlation is at 40 cm depth. The spectral reflectivity of red edge region (680-760 nm) which is relatively sensitive and stable, can better reflect the growth status of the plant, and the reflectivity variation per unit wavelength in this region increased at first and then decreased. The trend of polynomial regression curve of red edge parameters and soil moisture of different depth (0-60 cm) is similar, which shows that soil moisture of 0 and 20 cm increased at first and then decreased, but that of 40 cm and 60cm was opposite.