Recent advances in direct air capture by adsorption.

Significant progress has been made in direct air capture (DAC) in recent years. Evidence suggests that the large-scale deployment of DAC by adsorption would be technically feasible for gigatons of CO2 capture annually. However, great efforts in adsorption-based DAC technologies are still required. This review provides an exhaustive description of materials development, adsorbent shaping, in situ characterization, adsorption mechanism simulation, process design, system integration, and techno-economic analysis of adsorption-based DAC over the past five years; and in terms of adsorbent development, affordable DAC adsorbents such as amine-containing porous materials with large CO2 adsorption capacities, fast kinetics, high selectivity, and long-term stability under ultra-low CO2 concentration and humid conditions. It is also critically important to develop efficient DAC adsorptive processes. Research and development in structured adsorbents that operate at low-temperature with excellent CO2 adsorption capacities and kinetics, novel gas-solid contactors with low heat and mass transfer resistances, and energy-efficient regeneration methods using heat, vacuum, and steam purge is needed to commercialize adsorption-based DAC. The synergy between DAC and carbon capture technologies for point sources can help in mitigating climate change effects in the long-term. Further investigations into DAC applications in the aviation, agriculture, energy, and chemical industries are required as well. This work benefits researchers concerned about global energy and environmental issues, and delivers perspective views for further deployment of negative-emission technologies.

Target-Activated, Light-Actuated Three-Dimensional DNA Walker Nanomachine for Amplified miRNA Detection.

Accurate analysis of microRNA (miRNA) is promising for elucidation of cancer processes and therapeutic effects. In this study, we reported a new target-activated, light-actuated three-dimensional (3D) DNA walker on gold nanoparticles for sensitive detection of miRNA using pyrene-incorporated DNAzyme analogues. In this design, the target miRNA activated the 3D DNA walker system to releases the walking arm. Then, under ultraviolet light irradiation, the pyrene DNAzyme on the walking arm would consecutively cleave the disulfide bonds of substrate strands and recover the fluorescence signal, thus achieving the amplified miRNA detection. The sophisticated design of the light-actuated 3D DNA walker was systematically investigated. Furthermore, this strategy could also be employed for miRNA analysis in serum samples with satisfactory reproducibility. Notably, the proposed light-actuated 3D DNA walker-based technique eliminated the need of enzymes, cofactors, and RNA backbones, thereby significantly improving the stability and efficiency. Overall, the light-actuated 3D DNA walker-based strategy enabled facile, sensitive, and specific detection of miRNA and provided new perspectives in diagnostics.

1.3 kV Vertical GaN-Based Trench MOSFETs on 4-Inch Free Standing GaN Wafer.

In this work, a vertical gallium nitride (GaN)-based trench MOSFET on 4-inch free-standing GaN substrate is presented with threshold voltage of 3.15 V, specific on-resistance of 1.93 mΩ·cm2, breakdown voltage of 1306 V, and figure of merit of 0.88 GW/cm2. High-quality and stable MOS interface is obtained through two-step process, including simple acid cleaning and a following (NH4)2S passivation. Based on the calibration with experiment, the simulation results of physical model are consistent well with the experiment data in transfer, output, and breakdown characteristic curves, which demonstrate the validity of the simulation data obtained by Silvaco technology computer aided design (Silvaco TCAD). The mechanisms of on-state and breakdown are thoroughly studied using Silvaco TCAD physical model. The device parameters, including n--GaN drift layer, p-GaN channel layer and gate dielectric layer, are systematically designed for optimization. This comprehensive analysis and optimization on the vertical GaN-based trench MOSFETs provide significant guide for vertical GaN-based high power applications.

Target-driven assembly of DNAzyme probes for simultaneous electrochemical detection of multiplex microRNAs.

In this work, we employed target-driven assembly of a Mg2+-dependent DNAzyme to develop an ultrasensitive electrochemical biosensor for the simultaneous detection of miRNA-21 and miRNA-141. The target miRNAs could hybridize with two partial DNAzymes, facilitating the formation of a stable and active Mg2+-dependent DNAzyme. With the help of the Mg2+ cofactor, the DNAzyme could circularly cleave the ferrocene (Fc) or methylene blue (MB) labelled hairpin probes and release Fc and MB labels from the electrode surface, which could significantly amplify the current suppression to achieve multiple detection of small amounts of miRNA-21 and miRNA-141. This electrochemical biosensor showed high sensitivity and selectivity for the simultaneous detection of miRNA-21 and miRNA-141. Furthermore, the proposed method was also successfully applied for the determination of miRNA-21 and miRNA-141 from diluted serum samples. Overall, the proposed sensor showed several considerable advantages including simple preparation, high sensitivity, and enzyme-free signal amplification. Therefore, the proposed electrochemical biosensor could be used as a highly efficient amplification strategy for simultaneous detection of various miRNA biomarkers in bioanalysis and clinical diagnostics.

Reducing PD-L1 expression with a self-assembled nanodrug: an alternative to PD-L1 antibody for enhanced chemo-immunotherapy.

The binding between the immune checkpoints, programmed cell death ligand 1 (PD-L1) and programmed cell death 1 (PD-1), compromises T-cell-mediated immune surveillance. Immune checkpoint therapy using immune checkpoint inhibitors (ICIs) to block PD-L1 on cancer cell membrane or PD-1 on activated T cell membrane can restore antitumor function of T cell. However, the intracellular expression of PD-L1 and its active redistribution to cancer cell membrane may impair the therapeutic benefits of ICIs. To address this issue, herein we develop a nanodrug (MS NPs) capable of reducing PD-L1 expression and enhancing antitumor effects. Methods: The nanodrug was self-assembled from immunoadjuvant metformin (Met, an old drug) and anticancer agent 7-ethyl-10-hydroxycamptothecin (SN38) via hydrogen bonds and electrostatic interactions. A series of experiments, including the characterization of MS NPs, the validation of MS NPs-mediated down-regulation of PD-L1 expression and in vitro therapeutic effect, the MS NPs-mediated in vivo chemo-immunotherapy and tumor metastasis inhibition were carried out. Results: Different from ICIs that conformationally block PD-L1 on cancer cell membrane, MS NPs directly reduced the PD-L1 level via metformin to achieve immunotherapy. Therefore, MS NPs showed enhanced chemo-immunotherapy effect than its counterparts. MS NPs were also effective in inhibiting tumor metastasis by remodeling the extracellular matrix and restoring immune surveillance. Additionally, no obvious toxicity was observed in major organs from MS NPs-treated mice and a high survival rate of mice was obtained after MS NPs treatment. Conclusion: We have designed nanodrug MS NPs by self-assembly of the immunoadjuvant Met and the anticancer agent SN38 for combined immunotherapy and chemotherapy. MS NPs might break the deadlock of antibody-based ICIs in immunotherapy, and repurposing old drug might provide a new perspective on the development of novel ICIs.

Evaluation of heavy metal element detection in municipal solid waste incineration fly ash based on LIBS sensor.

Heavy metal elements are the main pollutants in municipal solid waste incineration (MSWI) fly ash, the online detection of heavy metals in MSWI fly ash could benefit its subsequent solidification treatment and land-filling. In this paper, laser induced breakdown spectroscopy (LIBS) was introduced to the rapid measurement of heavy metal elements in MSWI fly ash. Considering the serious matrix effect in MSWI fly ash, the multiple linear regression model combined with internal standard method was used to establish the calibration curves of heavy metals. Validated samples were used to evaluate the performance of quantitative analysis models. The results show that linear regression coefficients (R2) of the calibration curves for Cd, Cr, Cu, Pb, Zn are 0.981, 0.988, 0.968, 0.978 and 0.993, respectively. The average relative error of the prediction results are from 6.8 to 20.3%. The detection limits of the heavy metal content are Cd (11.13 µg/g), Cr (44.87 µg/g), Cu (36.18 µg/g), Pb (10.83 µg/g), Zn (12.27 µg/g), respectively, which are far below those required in the Standard for Pollution Control on the Landfill Site of Municipal Solid Waste (GB16889-2008). All results indicate the great potential of LIBS sensor for online rapid detection of heavy metals in MSWI fly ash.