[Technology of advanced glycation endproducts fluorescence measurement and precorrection].

The necessity to measure advanced glycation endproducts (AGE) was analyzed in the present paper, and the apparatus for measuring skin autofluorescence was also designed making use of the special excitation spectrum and emission spectrum. A portion of tissue at the volar side of the arm of individuals (11 diabetes and 19 control subjects) was illuminated with excitation light, i.e., monochromatic light around 370 nm, and the emission spectrum was detected on the skin of control subjects and diabetic patients respectively. All measurements were performed at room temperature in a semi-dark environment. It can be seen that different sites of an individual could lead to different results, and the color can also affect the results. The technology of fluorescence precorrection was applied in order to get rid of the influence of noise, different site of skin, the color of skin etc. The result indicates that the technology of precorrection is of avail and the repetitiveness is well.

[Design of advanced glycation endproducts fluorescence spectrum detecting system].

The method of making use of the technique of fluorescence spectrum to detect advanced glycation endproducts is discussed in the present pape. The emphasis is on the principle and structure of the fluorescence spectrum detecting system. In the end, the authors made use of the system to detect the excitation spectrum at the wavelengths of 365nm, 370nm, 375nm, 380nm and 385nm, respectively, and the authors found that 375nm is the best excitation wavelength. At the same time, the emission spectrum was also detected on the skin of nondiabetic people and patients with diabetes respectively. The result of the experiment indicates that there is a difference distinctly at about 450nm between them, and has proved the feasibility of the system. The detecting system does not need collecting blood sample, is a noninvasive detection technology, and avoids pain and infection to the patients. The process of detection is very rapid and convenient, and the repetitiveness is well. The patient can benefit from it to forecast and diagnose the state of illness such as diabetes, decrepitude and oxidative stress etc conveniently.