Enhancing the Thermotolerance of Isochrysis zhangjiangensis Through Co-culturing With Algoriphagus marincola.

Isochrysis zhangjiangensis is an important microalgal species used as bait in aquaculture. However, its optimal cultivation temperature is around 25 °C, limiting its use in summer when temperature is higher. To overcome this limitation, we aimed to develop a consortia of I. zhangjiangensis and bacteria that are more resistant to heat stress. Here, six thermotolerance-promoting bacterial strains were isolated from the culture of a heat-tolerant mutant strain of I. zhangjiangensis (IM), and identified as Algoriphagus marincola, Nocardioides sp., Pseudidiomarina sp., Labrenzia alba, Nitratireductor sp., and Staphylococcus haemolyticus. Further, co-culturing I. zhangjiangensis with A. marincola under high temperature conditions increased cell density, chlorophyll a, PSII maximum photochemical efficiency (Fv/Fm), and soluble protein content of microalgae. The presence of A. marincola positively influenced the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and total antioxidant capacity (T-AOC) in I. zhangjiangensis cells, while concurrently reducing the levels of reactive oxygen species (ROS). Additionally, gene expression studies confirmed that co-culturing with A. marincola upregulated the expression of antioxidant-related genes (sod and pod) and stress tolerance genes (heat shock protein genes). Our findings indicate that A. marincola effectively helps I. zhangjiangensis withstand high temperature stress, leading to improved yield of microalgae during high temperature conditions. The thermotolerance-promoting bacteria can be exploited as potential inoculants for enhancing the productivity and sustainability of bait microalgae in aquaculture.

Three Experimental Common High-Risk Procedures: Emission Characteristics Identification and Source Intensity Estimation in Biosafety Laboratory.

Biosafety laboratory is an important place to study high-risk microbes. In biosafety laboratories, with the outbreak of infectious diseases such as COVID-19, experimental activities have become increasingly frequent, and the risk of exposure to bioaerosols has increased. To explore the exposure risk of biosafety laboratories, the intensity and emission characteristics of laboratory risk factors were investigated. In this study, high-risk microbe samples were substituted with Serratia marcescens as the model bacteria. The resulting concentration and particle size segregation of the bioaerosol produced by three experimental procedures (spill, injection, and sample drop) were monitored, and the emission sources' intensity were quantitatively analyzed. The results showed that the aerosol concentration produced by injection and sample drop was 103 CFU/m3, and that by sample spill was 102 CFU/m3. The particle size of bioaerosol is mainly segregated in the range of 3.3-4.7 µm. There are significant differences in the influence of risk factors on source intensity. The intensity of sample spill, injection, and sample drop source is 3.6 CFU/s, 78.2 CFU/s, and 664 CFU/s. This study could provide suggestions for risk assessment of experimental operation procedures and experimental personnel protection.

Modeling the infection risk and emergency evacuation from bioaerosol leakage around an urban vaccine factory.

Mounting interest in modeling outdoor diffusion and transmission of bioaerosols due to the prevalence of COVID-19 in the urban environment has led to better knowledge of the issues concerning exposure risk and evacuation planning. In this study, the dispersion and deposition dynamics of bioaerosols around a vaccine factory were numerically investigated under various thermal conditions and leakage rates. To assess infection risk at the pedestrian level, the improved Wells-Riley equation was used. To predict the evacuation path, Dijkstra's algorithm, a derived greedy algorithm based on the improved Wells-Riley equation, was applied. The results show that, driven by buoyancy force, the deposition of bioaerosols can reach 80 m on the windward sidewall of high-rise buildings. Compared with stable thermal stratification, the infection risk of unstable thermal stratification in the upstream portion of the study area can increase by 5.53% and 9.92% under a low and high leakage rate, respectively. A greater leakage rate leads to higher infection risk but a similar distribution of high-risk regions. The present work provides a promising approach for infection risk assessment and evacuation planning for the emergency response to urban bioaerosol leakage.

Bioaerosol distribution characteristics and potential SARS-CoV-2 infection risk in a multi-compartment dental clinic.

Dental clinics have a potential risk of infection, particularly during the COVID-19 pandemic. Multi-compartment dental clinics are widely used in general hospitals and independent clinics. This study utilised computational fluid dynamics to investigate the bioaerosol distribution characteristics in a multi-compartment dental clinic through spatiotemporal distribution, working area time-varying concentrations, and key surface deposition. The infection probability of SARS-CoV-2 for the dental staff and patients was calculated using the Wells-Riley model. In addition, the accuracy of the numerical model was verified by field measurements of aerosol concentrations performed during a clinical ultrasonic scaling procedure. The results showed that bioaerosols were mainly distributed in the compartments where the patients were treated. The average infection probability was 3.8% for dental staff. The average deposition number per unit area of the treatment chair and table are 28729 pcs/m2 and 7945 pcs/m2, respectively, which creates a possible contact transmission risk. Moreover, there was a certain cross-infection risk in adjacent compartments, and the average infection probability for patients was 0.84%. The bioaerosol concentrations of the working area in each compartment 30 min post-treatment were reduced to 0.07% of those during treatment, and the infection probability was <0.05%. The results will contribute to an in-depth understanding of the infection risk in multi-compartment dental clinics, forming feasible suggestions for management to efficiently support epidemic prevention and control in dental clinics.

Droplet aerosols transportation and deposition for three respiratory behaviors in a typical negative pressure isolation ward.

Negative pressure isolation wards could provide safety for health care workers (HCWs) and patients infected with SARS-CoV-2. However, respiratory behavior releases aerosols containing pathogens, resulting in a potential risk of infection for HCWs. In this study, the spatiotemporal distribution of droplet aerosols in a typical negative pressure isolation ward was investigated using a full-scale experiment. In this experiment, artificial saliva was used to simulate the breathing behavior, which can reflect the effect of evaporation on droplet aerosols. Moreover, numerical simulations were used to compare the transport of droplet aerosols released by the three respiratory behaviors (breathing, speaking, and coughing). The results showed that droplet aerosols generated by coughing and speaking can be removed and deposited more quickly. Because reduction in the suspension proportion per unit time was much higher than that in the case of breathing. Under the air supply inlets, there was significant aerosol deposition on the floor, while the breathing area possessed higher aerosol concentrations. The risk of aerosol resuspension and potential infection increased significantly when HCWs moved frequently to these areas. Finally, more than 20% of the droplet aerosols escaped from the ward when the number of suspended aerosols in the aerosol space was 1%.

Seasonal dynamics of airborne culturable fungi and its year-round diversity monitoring in Dahuting Han Dynasty Tomb of China.

Dahuting Han Dynasty Tomb, famous for its magnificent and realistic murals in China, was selected for a year-long study as the research target in relation to microbiological degradation of cultural heritage. This dissertation has investigated the predominant genera of the airborne fungal microbial community in Dahuting and analyzed the seasonal distribution characteristics and temporal-spatial particle size distribution of the fungi in a tomb environment. The combination of culture-dependent and high-throughput sequencing methods was utilized for counting the collected fungi and identifying the strains. Results showed that seasonal dynamics significantly affect the fungal concentration, with higher-level concentrations observed in spring and autumn. However, seasonal variation has little effect on the fungal particle size distribution characteristic trend, and the higher concentration invariably appeared in stage IV to VI (0.65-3.3 µm) of the Andersen six-stage sieve impactor. The ITS (Internal Transcribed Spacer) rRNA gene-based sequences disclosed a high airborne culturable fungal abundance, dominated by Talaromyces spp. (20%-38.4%), followed by Aureobasidium spp. (19.4%-25.6%), Penicillium spp. (10.8%-23.9%) and Aspergillus spp. (8.2%-23.1%). Our research provides valuable information for reasonable protection measures and scientific prevention work of the murals in Dahuting Han Dynasty Tomb.

RNA-seq Insights Into the Impact of Alteromonas macleodii on Isochrysis galbana.

Phycospheric bacteria may be the key biological factors affecting the growth of algae. However, the studies about interaction between Isochrysis galbana and its phycospheric bacteria are limited. Here, we show that a marine heterotrophic bacterium, Alteromonas macleodii, enhanced the growth of I. galbana, and inhibited non-photochemical quenching (NPQ) and superoxide dismutase (SOD) activities of this microalgae. Further, we explored this phenomenon via examining how the entire transcriptomes of I. galbana changed when it was co-cultured with A. macleodii. Notable increase was observed in transcripts related to photosynthesis, carbon fixation, oxidative phosphorylation, ribosomal proteins, biosynthetic enzymes, and transport processes of I. galbana in the presence of A. macleodii, suggesting the introduction of the bacterium might have introduced increased production and transport of carbon compounds and other types of biomolecules. Besides, the transcriptome changed largely corresponded to reduced stress conditions for I. galbana, as inferred from the depletion of transcripts encoding DNA repair enzymes, superoxide dismutase (SOD) and other stress-response proteins. Taken together, the presence of A. macleodii mainly enhanced photosynthesis and biosynthesis of I. galbana and protected it from stress, especially oxidative stress. Transfer of fixed organic carbon, but perhaps other types of biomolecules, between the autotroph and the heterotroph might happen in I. galbana-A. macleodii co-culture. The present work provides novel insights into the transcriptional consequences of I. galbana of mutualism with its heterotrophic bacterial partner, and mutually beneficial associations existing in I. galbana-A. macleodii might be explored to improve productivity and sustainability of aquaculture algal rearing systems.

Green technological approach to synthesis hydrophobic stable crystalline calcite particles with one-pot synthesis for oil-water separation during oil spill cleanup.

The process of separating oil and water from oil/water mixtures is an attractive strategy to answer the menace caused by industrial oil spills and oily wastewater. In addition, water coproduced during hydrocarbon exploitation, which can be an economic burden and risk for freshwater resources, can become an important freshwater source after suitable water-oil separation. For oil-water separation purposes, considerable attention has been paid to the preparation of hydrophobic-oleophilic materials with modified surface roughness. However, due to issues of thermodynamic instability, costly and complex methods as well as lack of ecofriendly compounds, most of hydrophobic surface modified particles are of limited practical application. The study presents a facile procedure, to synthesize crystalline particles of calcite, which is the most stable polymorph of CaCO3 from industrial CaCO3 using oleic acid as an additive in a one-pot synthesis method. The XRD results show that the synthesized particles were a well-crystallized form of calcite. The FTIR results reflect the appearance of the alkyl groups from the oleic acid in synthesized particles which promotes the production of calcite with 'rice shape' (1.64 µm) (aggregated by spherical nanoparticle of 19.56 nm) morphology with concomitant changes in its surface wettability from hydrophilic to hydrophobic. The synthesized particles exhibited near to super hydrophobicity with ∼99% active ratio and a contact angle of 143.8°. The synthesized hydrophobic calcite particles had an oleophilic nature where waste diesel oil adsorption capacity of synthesized calcium carbonate (HCF) showed a very high (>99%) and fast (7 s) oil removal from oil-water mixture. The functional group of long alkyl chain including of CO bounds may play critical roles for adsorption of diesel oils. Moreover, the thermodynamically stable crystalline polymorph calcite (compared to vaterite) exhibited excellent recyclability. The isothermal study reflects the comparatively high value of correlation coefficient (R2 = 0.94) for the Langmuir isotherm compared to those of the Freundlich isotherm (R2 = 0.82) showed that the adsorption of diesel oil onto the hydrophobic CaCO3 adsorbent was much better described by the Langmuir isotherm. The kinetics study of second-order rate expression (R2 = 0.99) more fitted with the experimental data compare to first-order model (R2 = 0.92). The synthesized calcite exhibited a significant dual oleophilic and hydrophobic nature that can be applicable for oil adsorption/or removal purpose in oil contaminated areas in environment and/or industrial oily wastewater for green, simple, and inexpensive environmental cleanup.