ABSTRACT
We isolated a strain of Staphylococcus nepalensis from Nasonia vitripennis and presented the draft genome sequence of this strain. This research was conducted at the Institute of Zoology, Chinese Academy of Sciences (Beijing, China). The genome spans 2,910,033 bp, distributed over 144 contigs, with a G+C content of 33.33%.
ABSTRACT
Providencia stuartii prov-sta1 is a prevalent Gram-negative bacterium and dominant in the wasp Nasonia vitripennis. In this study, we present the draft genome sequence of P. stuartii prov-sta1, and the genome size is 4,380,152 bp in 183 contigs with a G+C content of 41.34%.
ABSTRACT
Mosquito-borne diseases like dengue and malaria cause a significant global health burden. Unfortunately, current insecticides and environmental control strategies aimed at the vectors of these diseases are only moderately effective in decreasing disease burden. Understanding and manipulating the interaction between the mosquito holobiont (i.e., mosquitoes and their resident microbiota) and the pathogens transmitted by these mosquitoes to humans and animals could help in developing new disease control strategies. Different microorganisms found in the mosquito's microbiota affect traits related to mosquito survival, development, and reproduction. Here, we review the physiological effects of essential microbes on their mosquito hosts; the interactions between the mosquito holobiont and mosquito-borne pathogen (MBP) infections, including microbiota-induced host immune activation and Wolbachia-mediated pathogen blocking (PB); and the effects of environmental factors and host regulation on the composition of the microbiota. Finally, we briefly overview future directions in holobiont studies, and how these may lead to new effective control strategies against mosquitoes and their transmitted diseases.
Subject(s)
Culicidae , Malaria , Microbiota , Wolbachia , Animals , Humans , Mosquito Vectors , Microbiota/physiology , Wolbachia/physiologyABSTRACT
[This corrects the article DOI: 10.3389/fmicb.2021.758794.].
ABSTRACT
Wolf spiders (Lycosidae) are crucial component of integrated pest management programs and the characteristics of their gut microbiota are known to play important roles in improving fitness and survival of the host. However, there are only few studies of the gut microbiota among closely related species of wolf spider. Whether wolf spiders gut microbiota vary with habitats remains unknown. Here, we used shotgun metagenomic sequencing to compare the gut microbiota of two wolf spider species, Pardosa agraria and P. laura from farmland and woodland ecosystems, respectively. The results show that the gut microbiota of Pardosa spiders is similar in richness and abundance. Approximately 27.3% of the gut microbiota of P. agraria comprises Proteobacteria, and approximately 34.5% of the gut microbiota of P. laura comprises Firmicutes. We assembled microbial genomes and found that the gut microbiota of P. laura are enriched in genes for carbohydrate metabolism. In contrast, those of P. agraria showed a higher proportion of genes encoding acetyltransferase, an enzyme involved in resistance to antibiotics. We reconstructed three high-quality and species-level microbial genomes: Vulcaniibacterium thermophilum, Anoxybacillus flavithermus and an unknown bacterium belonging to the family Simkaniaceae. Our results contribute to an understanding of the diversity and function of gut microbiota in closely related spiders.
ABSTRACT
The complete mitochondrial genome sequence of Desis martensi (L. Koch, 1872) was reported. In this study, we sequenced, assembled and annotated the mitochondrial genome of Desis martensi using next-generation sequencing (NGS). The sequence was 14,662 base pairs (bp) in length and consisted of 37 mitochondrial genes (13 protein-coding genes, 22 transfer RNAs, two ribosomal RNA genes). The overall base composition of the genome showed slightly A + T bias, AT content (77.2%) higher than GC content (22.9%). The phylogenetic analyses based on 13 protein-coding genes indicated that the family Desidae belonged to the Retrolateral Tibial Apophysis (RTA) clade in Araneae.
ABSTRACT
The complete mitochondrial genome sequence of a desert wolf spider, Halocosa hatanensis (Lycosidae) was reported. The circular molecule is 14,257 base pairs (bp) in length and consisted of 77.8% AT nucleotides, containing two ribosomal RNA genes, 22 transfer RNAs, 13 protein-coding genes (PCGs), 1 control region, and 1 AT-rich region. The length of 13 PCGs is 10,782 bp in total, encoding 3638 amino acids. The phylogenetic analyses upon mitogenomes proves its relatively basal position within Lycosidae. It would provide further evolutionary research data for the wolf spider family Lycosidae.
