ABSTRACT
In this study, the complete mitogenome sequence of the cryptic "lineage A" big-fin reef squid, Sepioteuthis lessoniana (Cephalopoda: Loliginidae) has been sequenced by the next-generation sequencing method. The assembled mitogenome consists of 16,605 bp, which includes 13 protein-coding genes, 22 transfer RNAs, and 2 ribosomal RNAs genes. The overall base composition of "lineage A" S. lessoniana is 37.5% for A, 17.4% for C, 9.1% for G, and 35.9% for T and shows 87% identities to "lineage C" S. lessoniana. It is also noticed by its high T + A content (73.4%), two non-coding regions with TA tandem repeats. The complete mitogenome of the cryptic "lineage A" S. lessoniana provides essential and important DNA molecular data for further phylogeography and evolutionary analysis for big-fin reef squid species complex.
Subject(s)
Decapodiformes/classification , Decapodiformes/genetics , Genome, Mitochondrial , Animals , Base Composition , Codon , Evolution, Molecular , Genes, Mitochondrial , Genome Size , Open Reading Frames , Phylogeny , Sequence Analysis, DNA , Whole Genome SequencingABSTRACT
In this study, we used the next-generation sequencing method to determine the complete mitogenome of Japanese swallow angelfish (Genicanthus semifasciatus) and Ornate angelfish (Genicanthus bellus) for the first time. The assembled mitogenome, 16,722 bp and 16,734 bp in length, shows 95% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop contains length of 1013 bp in G. semifasciatus and 1030 bp in G. bellus, and is located between tRNA-Pro and tRNA-Phe. The overall GC content is 46.4% for G. semifasciatus and 46.6% for G. bellus. The complete mitogenome of Japanese swallow angelfish and Ornate angelfish determined in this study provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for marine angelfish.
Subject(s)
Cypriniformes/genetics , Genome, Mitochondrial/genetics , Animals , Base Composition/genetics , Cypriniformes/classification , Gene Order/genetics , High-Throughput Nucleotide Sequencing , Phylogeny , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
The Leopard whipray (Himantura leoparda) and Blue-spotted stingray (Neotrygon kuhlii) are distributed in the Indian and West Pacific Ocean and considered as complex species based on morphological and molecular evidences. In this study, we used the next-generation sequencing method to decode two complete mitogenomes of H. leoparda and N. kuhlii. The assembled mitogenome, consisting lengths of 17,690 bp for H. leoparda and 17,974 bp for N. kuhlii, shows 78% identity to each other. Both mitogenomes follow the typical vertebrate arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region of D-loop. D-loop with the lengths 1931 bp (H. leoparda) and 2243 bp (N. kuhlii) is located between tRNA-Pro and tRNA-Phe. The overall GC content is 40.3% for H. leoparda and 39.8% for N. kuhlii. The complete mitogenome of H. leoparda and N. kuhlii provides essential and important DNA molecular data for further phylogenetic and evolutionary analyses for stingray species complex.
