Responses of the grass Paspalum distichum L. to Hg stress: A proteomic study.

Paspalum distichum L. was tested to evaluate its ability to phytoremediate mercury (Hg) contaminated soil over a 60-d period by analysis of the total Hg concentrations in roots and leaves. Hg concentration in Hg-contamination soil decreased by 70.0 µg g-1 after 60 day of grass cultivation and Hg was readily taken up by the roots (4.51 ±â€¯1.90 µg g-1) rather than the leaves (0.35 ±â€¯0.02 µg g-1). In addition, a comparative proteomic study was performed to unravel the protein expression involved in the Hg stress response in P. distichum L. A total of 49 proteins were classified as differentially proteins in the roots by the 'top three' proteomic analysis, of which 32 were up-regulated and 17 down-regulated in response to Hg stress. These changed proteins were classified by gene ontology analysis into five complex molecular functions involving photosynthesis and energy metabolism (31%), oxidative stress (14%), protein folding (16%), sulfur compound metabolism (10%), metal binding, and ion transport (29%). Moreover, the protein expression patterns were consistent with the metabolism pathway results. Overall, the results contribute to our understanding of the molecular mechanisms of the Hg response in P. distichum and we propose a theoretical basis for the phytoremediation of Hg-contaminated soils.

Mercury methylation by Geobacter metallireducens GS-15 in the presence of Skeletonema costatum.

In this study, bacterial mercury (Hg) methylation was investigated under the influence of red-tide algae of Skeletonema costatum (S. costatum). The distribution and speciation of total mercury (THg) and methylmercury (MeHg) were profiled by employing Geobacter metallireducens (G. metallireducens GS-15) as the methylating bacteria. G. metallireducens GS-15 showed different capabilities in methylating different inorganic forms of Hg(II) (HgCl2) and Hg(II)-Algae (HgCl2 captured by S. costatum) to MeHg. In the absence of S. costatum, a maximum methylation efficiency of 4.31 ±â€¯0.47% was achieved with Hg(II) of 1-100 µg L-1, while accelerated MeHg formation rate was detected at a higher initial Hg(II) concentration. In the presence of S. costatum, there were distinct changes in the distribution of THg and MeHg by altering the bioavailability of Hg(II) and Hg(II)-Algae. A larger proportion of THg tended to be retained by a higher algal biomass, resulting in decreased methylation efficiencies. The methylation efficiency of Hg(II) decreased from 3.01 ±â€¯0.10% to 1.01 ±â€¯0.01% with 10-mL and 250-mL algal media, and that of Hg(II)-Algae decreased from 0.83 ±â€¯0.13% to 0.22 ±â€¯0.01% with 10-mL and 250-mL Hg(II)-Algae media. Fourier transform infrared spectrometry, surface charge properties and elemental compositions of S. costatum were used to infer that amine, carboxyl and sulfonate functional groups were most likely to interact with Hg(II) through complexation and/or electrostatic attraction. These results suggest that red-tide algae may be an influencing factor on bacterial Hg methylation in eutrophic water bodies.

Inhibitory effects of Skeletonema costatum on mercury methylation by Geobacter sulfurreducens PCA.

Algae and mercury (Hg) are ubiquitous in marine environments. In this study, we investigated the effects of a typical marine algae of diatom Skeletonema costatum on Hg methylation by an iron-reducing bacterium of Geobacter sulfurreducens (G. sulfurreducens) PCA. In the absence of Skeletonema costatum, the bacterial MeHg production rate maximized at 104.06 ±â€¯11.7 ng L-1 h-1 with a high Hg level, while the highest methylation efficiency was achieved at a low Hg concentration. The existence of Skeletonema costatum greatly inhibited the capability of G. sulfurreducens PCA to methylate Hg. With the increase in algal biomass, there was a significant mitigation of MeHg formation and Hg0 release, leaving a considerable proportion of immobilized Hg2+ species (up to 47%) associated with algal cell materials. These results suggest that marine algae are crucial in determining the bioavailability of Hg contaminants and the methylating potential of G. sulfurreducens PCA.

The effects of aquaculture on mercury distribution, changing speciation, and bioaccumulation in a reservoir ecosystem.

A survey was conducted in a freshwater reservoir located in Guizhou province, south China, to investigate the influence of fish aquaculture activities on mercury (Hg) distribution, speciation change, and bioaccumulation. Water, sediment, and aquaculture fish samples were collected from aquaculture sites (AS) and the corresponding reference sites (RS). The results showed that total mercury (THg) concentration in overlying water in Wujiangdu reservoir reached 6.87 ± 14.9 ng L-1. THg concentration in a different layer follows the sequence: surface layer > bottom layer > three intermediate layers with significant difference. In addition, the total methylmercury (TMeHg) concentration in overlying water was 0.113 ± 0.211 ng L-1. The highest TMeHg was observed in the bottom layer. Both dissolved Hg (DHg) and dissolved methylmercury (DMeHg) accounted for more than 50% of THg and TMeHg in the top four layers of overlying water, respectively. In contrast, particulate Hg (PHg) and particulate methylmercury were the major portion of THg and TMeHg in bottom layer and reached to 67 and 58.7% of THg and TMeHg, respectively. Aquaculture activities were estimated to contribute an annual loading of approximately 69.8 ng g-1of THg yearly in the top 1 cm of sediment underneath the rafts due to unconsumed fish feed. The extra loading of THg in sediment may have potential to stimulate the release of Hg to the overlying water. Both DMeHg and DHg in sediment pore water and organic matter (OM) contents in the top 4 cm of AS were noticeably higher than RS, which indicated that the accumulation of OM due to aquaculture activities promoted MeHg production in the top surface of sediment pore water. No significant difference was noted between THg in RS (296 ± 104 ng g-1) and AS (274 ± 132 ng g-1) in the solid phase. In addition, the sediments were net sources of both MeHg and inorganic Hg. For Hg in fish tissues, THg and inorganic Hg (IHg) in the liver were significantly higher (THg: p < 0.001, F = 9.98; IHg: p < 0.001, F = 13.1) than those in the other organs. In contrast, MeHg concentration in the muscle was significantly higher than (p < 0.01, F = 4.83) that in the other organs.

A pilot study on health risk assessment based on body loadings of PCBs of lactating mothers at Taizhou, China, the world's major site for recycling transformers.

Our early study reported an extraordinarily high Estimated Daily Intake (EDI) of PCBs of lactating mothers from Taizhou, Zhejiang Province, China (based on a food consumption survey and food basket analysis). The EDI well exceeded the intake limit stipulated by FAO/WHO 70 pg TEQ/kg body weight (bw)/month. The present pilot study provided further information on PCBs body burden in lactating mothers of Taizhou. The total PCBs detected in human milk, placenta and hair samples of these lactating mothers were 363 ng/g lipid, 224 ng/g lipid, and 386 ng/g dry wt. Respectively, three times higher than those samples collected from the reference site (Lin'an). Compared with the previous reported values in the 3rd WHO coordinated study, Taizhou topped the list of 32 countries/regions with regards to WHO-PCB-TEQ values of milk samples, which could be attributed to the relatively higher level of PCB-126 derived from electronic waste. In addition, the corresponding EDI of PCBs of Taizhou mothers (12.9 pg WHO-PCB-TEQ/kg bw/day) and infants (438 pg WHO-PCB-TEQ/kg) were derived from individual congener levels in human milk. The results were also higher than the tolerable daily intakes recommended by WHO (1-4 pg WHO-TEQ/kg bw/day) by 3 and 110 times, for mothers and infants, respectively. A more intensive epidemiological study on the potential health effects of e-waste recycling activities affecting both workers and residents seems to be of top priority, based on findings of this pilot study.

Mycorrhizal colonization status of lowland rice (Oryza sativa L.) in the southeastern region of China.

The accumulation, distribution, and speciation of contaminants, such as arsenic, in rice can be affected by soil microorganisms such as arbuscular mycorrhizal fungi (AMF). As a potential measure to control contaminant acquisition in rice, the status and performance of AMF in the field need to be investigated. Root samples of rice plants were collected in seven different cities in Guangdong, Jiangxi, Hubei, and Jiangsu Provinces in China in order to investigate the colonization rate of AMF. The total DNA of the roots was extracted, followed by PCR and sequencing, and further confirmed the existence of AMF. The highest colonization rates (19.5 ± 7.2%) were observed in samples from Huizhou City, Guangdong Province. Sequences of ribosomal DNA derived from Pingtan (PT) and Shuikou (SK) in Huizhou shared a similarity of 73 and 86% to Glomus cf. clarum Att894-7 (FM865542) and "uncultured fungus" (EF434122.1), respectively. The moisture tolerance of the AMF from different sources was tested by subjecting to different levels of water content in the soil. Only AMF from PT, SK, and LJ colonized rice under a condition of 100% of the soil water holding capacity (WHC), but not those isolated from upland plants. The AM colonization rate could be governed by the lighting conditions and temperature. AMF isolated in paddy fields has been shown to have more tolerance to moisture than other upland species. Radial oxygen loss (species and stress dependent) could be an essential factor influencing the colonization rate and requires more investigation.

Role of mariculture in the loading and speciation of mercury at the coast of the East China Sea.

The effects of mariculture on mercury (Hg) contamination and speciation in water, sediment and cultured fish in a typical mariculture zone located in Xiangshan bay, Zhejiang province, east China, were studied. Water, sediment and fish samples were collected from mariculture sites (MS) and from corresponding reference sites (RS) 2500 m away from the MS. The THg concentration in overlying water in Xiangshan bay reached as high as 16.6 ± 19.5 ng L-1, indicating that anthropogenic sources in this bay may contribution on Hg contamination in overlying water. Mariculture activities resulted in an increase in THg concentration in water from surface and bottom layers, which may be attributed to the discharge of domestic sewage and the accumulation of unconsumed fish feed and fish excreta in the benthic environment. Methylmercury (MeHg) concentrations in the bottom layer of overlying water and top surface layer of porewater underneath MS were higher than at RS, implying that mariculture activities promote Hg methylation in the interface between sediments and water. In addition, the concentrations of MeHg in sediment and porewater were significantly higher in summer than winter. It was observed that THg and MeHg contents in the muscle of blackhead seabream (Acanthopagrus schlegelii) (fed by the trash fish) were significantly higher (p < 0.001) than those in red snapper (Lutjanus campechanus) or perch (Perca fluviatilis) (fed by pellet fish feed). The THg and MeHg concentrations in the fish meat were closely related to the feeding mode, which indicate that fish feed rather than environmental media is the major pathway for Hg accumulation in fish muscle.

The effects of mariculture on heavy metal distribution in sediments and cultured fish around the Pearl River Delta region, south China.

Thirty-six sediment samples were collected from six mariculture sites and corresponding reference sites (approximately 200-300 m away from each mariculture site) to study the effects of mariculture on heavy metal: copper (Cu), zinc (Zn), chromium (Cr) and lead (Pb) distribution in sediments and cultured fish around the Pearl River Delta region, south China. The mean concentrations of Cu, Zn, Cr and Pb in all mariculture sediment samples were 109, 273, 99 and 33 mg/kg, compared with 63, 209, 56 and 23 mg/kg for reference sediment samples, respectively. The Pollution Load Index of sediment for each site was over 1, implying substantial heavy metal pollution. The results of principal component analysis and hierarchical clustering analysis indicate that marine aquaculture activities have enriched the surface sediments underneath mariculture rafts with Cu, Zn and Pb, possibly due to the accumulation of unconsumed fish feeds, fish excreta and antifouling paints (except Cr). Two kinds of fish feeds, feed pellets and trash fish commonly used in Hong Kong were analyzed. The concentrations of Zn and Cu contained in feed pellets were significantly higher (p < 0.05) than in trash fish due to addition of these elements as growth promoters during pellet production. However, the Pb content in trash fish (due to contamination) was significantly higher (p < 0.05) than in feed pellets. Three cultured fish species, namely red snapper (Lutjanus campechanus), orange-spotted grouper (Epinephelus coioides) and snubnose pompano (Trachinotus blochii), were collected from each mariculture site for metal analysis. Lead concentrations in 21 fish samples exceeded the Chinese safety guideline (0.5 mg/kg, GB18406.4-2001), indicating that Pb contamination in cultured fish would be a public health concern.

Human exposure to mercury in a compact fluorescent lamp manufacturing area: By food (rice and fish) consumption and occupational exposure.

To investigate human Hg exposure by food consumption and occupation exposure in a compact fluorescent lamp (CFL) manufacturing area, human hair and rice samples were collected from Gaohong town, Zhejiang Province, China. The mean values of total mercury (THg) and methylmercury (MeHg) concentrations in local cultivated rice samples were significantly higher than in commercial rice samples which indicated that CFL manufacturing activities resulted in Hg accumulation in local rice samples. For all of the study participants, significantly higher THg concentrations in human hair were observed in CFL workers compared with other residents. In comparison, MeHg concentrations in human hair of residents whose diet consisted of local cultivated rice were significantly higher than those who consumed commercial rice. These results demonstrated that CFL manufacturing activities resulted in THg accumulation in the hair of CFL workers. However, MeHg in hair were mainly affected by the sources of rice of the residents.

Human health risk assessment of soil dioxin/furans contamination and dioxin-like activity determined by ethoxyresorufin-O-deethylase bioassay.

The major objective of this study was to evaluate the human health risks of agricultural land use conversion to other purposes in Hong Kong, based on the levels of polychlorinated dibenzo-p-dioxin and polychlorinated dibenzofuran (PCDD/Fs) and determined dioxin-like activity in soil using ethoxyresorufin-O-deethylase (EROD) bioassay. Hazard quotient showed soils of open burning site (OBS) and electronic waste open burning site (EW (OBS)) exert a relatively higher non-cancer risk on adults (50.9 and 8.00) and children (407 and 64.0) via the pathway of accidental ingestion of soil particles than other types of land use. In addition, the levels of 17 PCDD/Fs congeners in OBS and EW (OBS) soils indicated high and moderate (1654 and 260 in one million people) cancer risks through the above pathway. Furthermore, the biologically derived TCDD concentrations (TEQbio) were also significantly correlated to the chemically derived toxic equivalent concentrations of dioxin-like chemicals (TEQcal (sum of chemically derived 2,3,7,8-TeCDD toxic equivalent concentrations (TEQPCDD/F) and chemically derived dioxin-like PAHs toxic equivalent concentrations (TEQPAH)) (r = 0.770, p <0.05). PCDD/Fs (95.4 to 99.9%) were the major stressor to the TEQcal in the soil samples, indicating higher concentrations of PCDD/Fs derived from chemical analyses may reflect a higher potency of inducing EROD activity.

Shark fin, a symbol of wealth and good fortune may pose health risks: the case of mercury.

There is a lack of information concerning human health risks due to exposure to mercury contained in shark fins, through dietary intake. Health risk assessment of shark fins, collected from 5 cities in China: Hong Kong (HK), Beijing, Shanghai (SH), Haikou (HN) and Wenzhou (WZ), was conducted, based on total mercury and methylmercury, and analyzed by a cold vapor atomic fluorescence spectrometry equipped with high-performance liquid chromatography, respectively. The results showed that 16.8 % samples from HK, 8.3 % from SH, 33.3 % from HN, and 16.8 % from WZ were regarded as unsafe for human consumption. Extremely high consumption rates of shark fins for an adult and a child (0.150 and 0.0807 kg/day, respectively), at 95th centile, samples from WZ demonstrated high non-cancer risks (adverse health effects) (Hazard Quotient = 16.0) on adults (aged 25-65), and samples from SH, HN and WZ also showed high non-cancer risks (Hazard Quotient = 12.9, 21.0, and 34.4, respectively) on children (aged 1-7). Consumption of shark fins may be detrimental to human health.

DDTs in mothers' milk, placenta and hair, and health risk assessment for infants at two coastal and inland cities in China.

This study is a one of the very few investigating the dichloro-diphenyl-trichloroethanes (DDTs) (summation of o,p'-DDE, p,p'-DDE, o,p'-DDD, p,p'-DDD, o,p'-DDT, and p,p'-DDT) in multiple human matrices in mothers' milk, placenta and hair collected from residents from two coastal cities: Guiyu (GY) and Taizhou (TZ) and one inland city: Lin'an (LA). TZ (milk: 360±319ng/g lipid wt.) showed significantly higher concentrations of DDTs than those from LA (milk: 190±131ng/g lipid wt.), whereas, concentrations of DDTs in GY (milk: 305±109ng/g lipid wt.) were in between TZ and LA. In addition, levels of DDTs in the human tissues from TZ (placenta: 122±109ng/g lipid wt.; hair: 79.9±215ng/g dry wt.) were significantly higher than those from Lin'an (placenta: 49.2±30.2ng/g lipid wt.; hair: 10.8±7.09ng/g dry wt.). The above concentrations of DDTs in milk exceeded the Codex Maximum Residue Limits/Extraneous Maximum Residue Limits for milk (20ng/g lipid wt. whole milk), indicating that the human milk samples were grossly polluted. The present study revealed that human specimens collected from the coastal city (TZ) were more contaminated with inland one (LA), based on the levels of DDTs contained in samples which may be due to the higher dietary exposure to DDTs via consumption of contaminated seafood. The estimated daily intakes of DDTs by GY, TZ and LA infants were 1.69±1.86, 1.48±0.79, and 0.95±0.73µg/kg body wt./day, respectively which did not exceed 10µg/kg body wt./day, the provisional tolerable daily intake proposed by the Food and Agriculture Organization/World Health Organization.

Dietary exposure to DDTs in two coastal cities and an inland city in China.

Dietary intakes of dichloro-diphenyl-trichloroethanes (DDTs) of residents from two coastal cities: Guiyu (GY) and Taizhou (TZ) and one inland city: Lin'an (HZ) were investigated by collecting 73 food items (divided into 9 food groups). The oriental weatherfish and white crab (both from TZ) contained higher DDTs (112±1.81 and 70.1±1.81 ng/g wet wt, respectively) than the maximum admissible concentration (50 ng/g wet wt) set by the European Union for human consumption. Furthermore, 40% of TZ seafood, 56% of GY and 30% of HZ freshwater fish exceeded the guideline for subsistence fish eaters for DDTs (14.4 ng/g wet wt) defined by the United States Environmental Protection Agency (US EPA). The estimated daily intakes of DDTs for TZ (52.1 ng/kg bw/day) and GZ (31.5 ng/kg bw/day) were significantly higher than for HZ (13.0 ng/g wet wt, p<0.05), these values were below the US EPA oral reference dose (500 ng/kg bw/day) and the Food and Agriculture Organization of the United Nations and the World Health Organization provisional tolerable daily intake (10,000 ng/kg bw/day).

Plasma mercury levels in Hong Kong residents: in relation to fish consumption.

Mercury exposure is of particular concern since mercury is a neurotoxin and the developing fetus is most sensitive to its adverse effect. Human blood is routinely used as an indicator for the evaluation of human exposure to Hg. To investigate Hg species in human plasma for Hong Kong residents and the relationship between fish consumption and Hg species in plasma, 151 plasma samples were analyzed for Hg species. The mean values of total Hg (THg) and methyl-mercury (MeHg) concentration in plasma were 0.62 and 0.28 µg/L, respectively. No significant differences were observed between females and males as well as among age groups. Fish consumption rate was significantly positively correlated with MeHg concentrations in plasma, which demonstrated that plasma could be a biomarker for human MeHg exposure. Two methods were used to estimate human MeHg exposure. One was based on fish MeHg content and fish consumption rate (EDI(Fish)), another was employed by converting MeHg concentration in blood to MeHg exposure amount (EDI(Blood)). A significant positive correlation was observed between EDI(Blood) and EDI(Fish), and no significant difference was found between EDI(Blood) and EDI(Fish). These results demonstrated that fish consumption was the major source of MeHg for humans.

Effects of salinity and humic acid on the sorption of Hg on Fe and Mn hydroxides.

The objective of this study was to investigate the influence of humic acid (HA) and salinity on adsorption of Hg on the amorphous and crystalline of iron and manganese hydroxides. The results show that the adsorption of Hg(2+) on Fe and Mn hydroxides was inhibited in marine system due to the formation of stable, nonsorbing aqueous HgCl(2) complexes in solution. Moreover, Cl(-) inhibited the Hg(2+) adsorption more severely on amorphous than crystalline hydroxides. The addition of HA inhibited Hg(2+) adsorption on Fe and Mn hydroxides in freshwater system might be attributed to the competition between Hg(2+) and HA on adsorption to Fe and Mn hydroxides. In contrast, the addition of HA promoted Hg(2+) adsorption on Fe and Mn hydroxides in the marine system, which might be due to the addition of humic acid resulted in the reaction between Cl(-) and HA, and therefore the reducing of Cl(-) promoted more Hg(2+) on Fe and Mn hydroxides. In addition, the influence of HA on Hg(2+) adsorption on Fe and Mn hydroxides are more visible for crystalline than amorphous hydroxides.

Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans via fish consumption and dioxin-like activity in fish determined by H4IIE-luc bioassay.

Dietary exposure to polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) via fish consumption in two major electronic (e) waste sites: Guiyu (GY), Guangdong Province and Taizhou (TZ), Zhejiang Province, and dioxin-like activity in fish determined by H4IIE-luc bioassay. In the present study, all fish were below EU's maximum allowable concentration in muscle of fish (4 pg WHO-TEQ/g wet wt), except crucian (4.28 pg WHO-TEQ/g wet wt) and silver carps (7.49 pg WHO-TEQ/g wet wt) collected from GY rivers. Moreover, the residual concentration in bighead carp collected from GY (2.15 pg WHO-TEQ/g wet wt) was close to the EU's action level (3 pg WHO-TEQ/g wet wt) which gives "early warning" to the competent authorities and operators to take measures to eliminate contamination. In addition, results indicated that the maximum human intake of PCDD/Fs via freshwater fish consumption in GY was 4.31 pg WHO-TEQ/kg bw/day, which exceeds the higher end of the tolerable daily intake recommended by the WHO, EC-SCF and JECFA (1-4, 2 and 2.3 pg WHO-TEQ/kg bw/day respectively). Furthermore, H4IIE-luc cell bioassay provides a very sensitive and cost-efficient screening tool for assessing the overall dioxin-like toxicity in the study, and is therefore valuable for high-throughput environmental monitoring studies.

Arsenic contamination in the freshwater fish ponds of Pearl River Delta: bioaccumulation and health risk assessment.

This study investigated the extent of arsenic (As) contamination in five common species of freshwater fish (northern snakehead [Channa argus], mandrarin fish [Siniperca chuatsi], largemouth bass [Lepomis macrochirous], bighead carp [Aristichthys nobilis] and grass carp [Ctenopharyngodon idellus]) and their associated fish pond sediments collected from 18 freshwater fish ponds around the Pearl River Delta (PRD). The total As concentrations detected in fish muscle and sediment in freshwater ponds around the PRD were 0.05-3.01 mg kg(-1) wet weight (w. wt) and 8.41-22.76 mg kg(-1) dry weight (d. wt), respectively. In addition, the As content was positively correlated (p < 0.05) to total organic carbon (TOC) contents in sediments. Biota sediment accumulation factor (BSAF) showed that omnivorous fish and zooplankton accumulated higher concentrations of heavy metals from the sediment than carnivorous fish. In addition, feeding habits of fish also influence As accumulation in different fish species. In this study, two typical food chains of the aquaculture ponds were selected for investigation: (1) omnivorous food chain (zooplankton, grass carp and bighead carp) and (2) predatory food chain (zooplankton, mud carp and mandarin fish). Significant linear relationships were obtained between log As and δ (15)N. The slope of the regression (-0.066 and -0.078) of the log transformed As concentrations and δ (15)N values, as biomagnifications power, indicated there was no magnification or diminution of As from lower trophic levels (zooplankton) to fish in the aquaculture ponds. Consumption of largemouth bass, northern snakehead and bighead carp might impose health risks of Hong Kong residents consuming these fish to the local population, due to the fact that its cancer risk (CR) value exceeded the upper limit of the acceptable risk levels (10(-4)) stipulated by the USEPA.

Urbanization increased metal levels in lake surface sediment and catchment topsoil of waterscape parks.

Lake surface sediment is mainly derived from topsoil in its catchment. We hypothesized that distribution of anthropogenic metals would be homogenous in lake surface sediment and the lake's catchment topsoil. Anthropogenic metal distributions (cadmium (Cd), copper (Cu), lead (Pb), and zinc (Zn)) in fourteen waterscape parks were investigated in surface sediments and catchment topsoils and possible source homogeneity was tested using stable Pb isotopic ratio analysis. The parks were located along an urbanization gradient consisting of suburban (SU), developing urban (DIU), developed urban (DDU), and central urban core (CUC) areas in Shanghai, China. Results indicated that surface lake sediments and catchment topsoils in the CUC parks were highly contaminated by the investigated anthropogenic metals. Total metal contents in surface sediment and topsoil gradually increased along the urbanization gradient from the SU to CUC areas. Generally, the surface sediments had greater total metal contents than their catchment topsoils. These results suggest that urbanization drives the anthropogenic metal enrichment in both surface sediment and its catchment topsoil in the waterscape parks. Soil fine particles (<63 µm) and surface sediments had similar enrichment ratios of metals, suggesting that surface runoff might act as a carrier for metals transporting from catchment to lake. Stable Pb isotope ratio analysis revealed that the major anthropogenic Pb source in surface sediment was coal combustion as in the catchment topsoil. Urbanization also correlated with chemical fractionation of metals in both surface sediment and catchment topsoil. From the SU to the CUC parks, amounts of labile metal fractions increased while the residual fraction of those metals remained rather constant. In short, urbanization in Shanghai drives anthropogenic metal distribution in environmental matrices and the sources were homogenous.

The effects of mariculture activities on the adsorption/desorption and chemical fractionations of mercury on sediments.

The aims of the present study were to investigate the effects of mariculture activities on inorganic mercury (Hg(2+)) adsorption/desorption on sediments and the distributions of newly adsorbed Hg(2+) on different chemical fractionations. The adsorption amount and binding energy of Hg(2+) on mariculture sediment (MS) were significantly higher (p<0.05) than reference sediment (RS). This may be explained by the strong complexation role that exists between Hg(2+) and organic matter (OM), which derived from unconsumed fish feed and fish metabolites. The reducible Hg(2+) in MS was significantly lower (p<0.01) than RS, which may have been caused by the decreasing amount of iron and manganese hydroxide in MS, lead to the decrease of Hg(2+) bound to them. On the contrary, the residual Hg(2+) was significantly higher (p<0.01) in MS than RS, which suggests that newly adsorbed Hg(2+) was more stable in MS than RS.

Health risk assessment of organochlorine pesticides with emphasis on DDTs and HCHs in abandoned agricultural soils.

The aim of this study was to evaluate the consequence of changing land use from agriculture land to other use purposes with respect to OCPs non-cancer and cancer risk on human health, based on concentrations of DDTs and HCHs in soils collected from 55 locations representing 12 different land use types. There were no non-cancer risks of DDTs and γ-HCHs on adults and children, and there were very low cancer risks of DDD, o,p'-DDE, DDT, α-HCH, ß-HCH, γ-HCH based on their total concentrations in all samples. Nonetheless, there were significant correlations of DDT to its metabolites (DDE and DDD) (r = 0.506 and r = 0.648) and DDE to DDD (r = 0.438) both at p < 0.01. OCP levels should be routinely monitored in different environmental media and food in order to verify whether there is fresh input. Their potential risks on human health should also be assessed.