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PLoS One ; 6(6): e21004, 2011.
Article in English | MEDLINE | ID: mdl-21698210

ABSTRACT

An immunomodulatory protein (IPAF) was purified and cloned from Anoectochilus formosanus, an Orchidaceae herbal plant in Asia. The major targeting immune cells of IPAF and its modulating effects toward B lymphocytes were investigated. Rapid amplification of cDNA ends (RACE) was conducted to clone the IPAF gene, and the obtained sequence was BLAST compared on the NCBI database. MACS-purified mouse T and B lymphocytes were stimulated with IPAF and the cell proliferation, activation, and Igs production were examined. IPAF comprised a 25 amino acids signal peptide and a 138 amino acids protein which was homologous to the lectins from Orchidaceae plant. IPAF selectively induced the cell proliferation in mouse splenic B lymphocytes but not T lymphocytes. The IPAF-induced B cells exhibited increased CD69 and MHC class II expression, and a dose- and time-dependent enhancement in IgM production. These results suggested potential benefits of IPAF to strengthen the humoral immunity.


Subject(s)
B-Lymphocytes/immunology , Immunoglobulin M/metabolism , Orchidaceae/metabolism , Plant Proteins/genetics , T-Lymphocytes/immunology , Amino Acid Sequence , Animals , Base Sequence , Blotting, Western , Cloning, Molecular , DNA, Complementary , Electrophoresis, Capillary , Electrophoresis, Polyacrylamide Gel , Lymphocyte Activation , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Molecular Sequence Data , Plant Proteins/physiology , Sequence Homology, Amino Acid
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