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1.
Article in Chinese | MEDLINE | ID: mdl-18230301

ABSTRACT

OBJECTIVE: To explore association genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation. METHODS: 182 workers exposed to radiation for at least one year with chromosomal damage were selected as cases based on a general health examination for all workers exposed to radiation in Tangshan city. The control group without chromosomal damage was matched to case by age (within 5 years), sex, work unit, type of exposed to radiation, cumulate serve length (within 1 year) according to 1:1. The micro whole blood cultivation was used for the chromosome analysis. The chromosome aberration type and rate were observed and counted. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to examine the genotype of three XPD loci (751, 312 and 156). RESULTS: The frequency of XPD 751 AA in cases was higher than that in controls (P < 0.05). The frequency of 751 allele in case group was statistically higher than that in the control groups (P < 0.05). No statistical difference was found in the frequencies of XPD 312 genotype and allele between the case and control group (P > 0.05). 156 mutant gene type in case group was higher than that in the control groups. The frequency of 156 A allele in case group were higher than that of the control groups (P < 0.05). The frequency of genotype with both 751AA and 156CA or 751AA and 156AA was higher in cases than that of controls (P < 0.05). CONCLUSION: XPD 751AA genotype is a possible risk factor for radiation-induced chromosomal damage. XPD 156 mutant gene type is a possible risk factor for radiation-induced chromosomal damage. Individuals with both XPD 751AA and 156 (CA+AA) genotypes are susceptible to radiation-induced chromosomal damage. No association of XPD 312 polymorphism with radiation-induced chromosomal damage is found.


Subject(s)
Occupational Exposure/adverse effects , Polymorphism, Restriction Fragment Length , Radiation , Xeroderma Pigmentosum Group D Protein/genetics , Adult , Case-Control Studies , Chromosome Aberrations/radiation effects , Female , Genetic Predisposition to Disease , Humans , Male , Middle Aged , Young Adult
2.
Article in Chinese | MEDLINE | ID: mdl-16978516

ABSTRACT

OBJECTIVE: To explore the relationship between polymorphisms of DNA repair gene XRCC1 and susceptibility to radiation injury. METHODS: In 1:1 case-control study, 113 abnormal chromosome workers exposed to ionizing radiation were selected as cases and 113 normal chromosome as controls who matched with case for sex, age (+/- 5 years), nation, type of work, the same or more but in 2 years work length and the same similar levels of the cumulative exposure radiation dose. Genotypes were analysed using PCR based restriction fragment length polymorphism techniques. RESULTS: The frequency of XRCC1 26304TT allele in case group (18.58%) was significantly higher than that in control group (7.08%), with OR for radiation damage being 3.47 (95% CI 1.43 - 8.44, P < 0.05). No association was observed between XRCC1 G27466A and G28152A and susceptibility to radiation injury. CONCLUSION: The mutation of XRCC1 C26304T is related with the susceptibility to radiation injury. The polymorphisms of XRCC1 G27466A and G28152A are not found to have association with abnormal chromosomes.


Subject(s)
DNA Repair , DNA-Binding Proteins/genetics , Genetic Predisposition to Disease , Radiation Injuries/genetics , Adult , Case-Control Studies , Chromosome Aberrations , Female , Gene Frequency , Genotype , Humans , Male , Middle Aged , Polymerase Chain Reaction , Polymorphism, Restriction Fragment Length , X-ray Repair Cross Complementing Protein 1
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